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Zero Nowhere
Beer Drinker



Registered: 01/29/18 
Posts: 1,471
Loc: Standing on the moon
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Re: Making shoeboxes [Re: Failboat]
#25043661 - 03/06/18 06:04 PM (5 years, 10 months ago) |
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Quote:
Quirkmeister92 said:
Quote:
Zero Nowhere said: Is there a benefit to using 1:4, as opposed to a 1:1?
It depends on the grain type and bulk sub, but why 1:4, why not just 1:2? Example: If you use grass seed then you have more noc points compared to large grain, but relativelt low water content and so using more coir makes sense as to supply more water.
I was going to use a 1:2 ratio but one of my jars had a spot that wasn't colonized yet, and I had to spawn tonight. So I did 1:4.
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Failboat
Fuck Up

Registered: 02/01/18
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Ah its 1:4 cause you rossed the uncolonized bit? If so then no worries
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Zero Nowhere
Beer Drinker



Registered: 01/29/18 
Posts: 1,471
Loc: Standing on the moon
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Re: Making shoeboxes [Re: Failboat]
#25043716 - 03/06/18 06:43 PM (5 years, 10 months ago) |
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I didn't toss it. I put that jar away to finish colonizing. I'll spawn it next week
Edited by Zero Nowhere (03/06/18 06:44 PM)
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Failboat
Fuck Up

Registered: 02/01/18
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Gotcha
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Failboat
Fuck Up

Registered: 02/01/18
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Re: Making shoeboxes [Re: Failboat]
#25043731 - 03/06/18 06:49 PM (5 years, 10 months ago) |
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Are these colonized enough to spawn? I am thinking 2 jars per shoebox at 1:1


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Shrooms4life
TrippyShaman



Registered: 06/08/17
Posts: 1,375
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Re: Making shoeboxes [Re: Failboat]
#25043780 - 03/06/18 07:17 PM (5 years, 10 months ago) |
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Quote:
Quirkmeister92 said: Are these colonized enough to spawn? I am thinking 2 jars per shoebox at 1:1



Look good to me man spawn away.
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Steevo
Just a cog in the death wheel



Registered: 03/02/17
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Re: Making shoeboxes [Re: Failboat]
#25043802 - 03/06/18 07:25 PM (5 years, 10 months ago) |
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Colonized enough? Either fully colonized or nothing imo. Why would you open a jar or bag of spawn that wasn't 100%
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Failboat
Fuck Up

Registered: 02/01/18
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Re: Making shoeboxes [Re: Steevo]
#25043975 - 03/06/18 08:36 PM (5 years, 10 months ago) |
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Quote:
Steevo said: Colonized enough? Either fully colonized or nothing imo. Why would you open a jar or bag of spawn that wasn't 100%

Duh, I was asking for your opinion as to if you consider that 100% or not. So if you would like to share...
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Humble Newcomer
Diddler de niรฑos



Registered: 03/12/17
Posts: 1,483
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Re: Making shoeboxes [Re: Failboat]
#25044189 - 03/06/18 10:40 PM (5 years, 10 months ago) |
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You'd have to isolate it wouldn't you?
Each clone you take is going to be the same several pairs of dikaryotic mycelia that are co habitating and co fruiting.
But if u took a clone to agar and let it grow out, notice the sectoring, and take each sector to an individual plate.
The best tubs that have thick "canopies" I'm pretty sure that's what they do. Your clone that u take may have 10 separate isolates that will need to all be fruited to see what each one is like (pf Tek makes a nice comparison method).
Once u have a good fruiting isolate u make all your quarts from that isolate and it will be extremely predictable and uniform.
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Humble Newcomer
Diddler de niรฑos



Registered: 03/12/17
Posts: 1,483
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Oh man there was more pages wtf.
That was in response to quirkmasters tub. To achieve uniformity.
Mushboy was talking about senescence, If u keep cloning a clone it will eventually get old and die. More likely it won't die but will fruit poorly and be contam prone.
When u have a good grow and want to clone that mushroom think of it as chopping off the arm of your favorite mush guy, and then your mushroom regrows its arm but the arm also regrows it's whole body. That's the stupid way I think of it. You don't grow a kid from an arm, u regrow the donor body.
You can do this over and over. And each one will be an exact duplicate because it is in fact the same organism.
But after a grow u take a mush and clone it on agar, now u put wedges to grains, grains to coir, wait, fruit, clone, back to agar back to grains... But all with the same old tired specimen. It will eventually stop performing well. Basically the same old guy doing grow after grow. And the organism is aging in real time a month or two each grow. Eventually he will produce less and less.
At that point u would have to take a spore print from your best performer.
That will be a parent to a new generation of kids (spores) and if the parent was a desired specimen like it was big well all the kids have a chance of being big too.
But smart human parents have stupid kids sometimes and red hair skips generations doesn't it, same with shrooms, so a big fat fruit will have millions of spores but not all will grow to be big. That's why we want to clone and maintain a culture when it's good and proven, to keep it.
So what's best is to have a master slants or similar to maintain your culture without it aging. You can keep it for life because it only comes out of stasis long enough for u to get a wedge out and put on a dish. Put master back up in fridge. It slows time way down, a year in the fridge may the same as two weeks at room temp or whatever. Now Let grow plate grow out and u get 30+ wedges from it for 30+ grain masters which makes 300+ quarts.
All from one wedge of the master which never aged bc it stays locked up in stasis
Edit - it seems many active growers don't bother with it, and I can see why, but in gourmet I have 3 or 4 different kinds of oysters and 2 shiitake already and I just started. Each oyster is different color and requires diff fruiting temps so they're seasonal and beautiful and $20/lb retail at farmers market and basically maintaining cultures becomes key. It's impossible to keep redoing each culture looking for good performers
Edited by Humble Newcomer (03/06/18 11:00 PM)
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Humble Newcomer
Diddler de niรฑos



Registered: 03/12/17
Posts: 1,483
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Also to quirkmasters, full colonization plus one week before spawning.
I don't think anyone could tell you by eye, full colonization is 100% colonized and then wait the extra week to ensure the middle is colonized but in reality it's more about letting the mycelium get a strong hold on the sub and also digest and pull energy from the sub to be at full defenses before u open jar and play around in open air. I think of it like a snake the myc completely covers the grain spawn as fast as it can bc it thinks other organisms will get it otherwise. It's a loose hold I think. And it can't absorb full nutrition just by touching it, it needs time to absorb like a rat in a snakes mouth
Open air is filthy u want your myc to have it's heels dug in and ready before u up the difficulty from babied in a jar with no competition all the way to your wife's yelling the baby's crying the bills are stacking up and you just lost your job.
Earlier may work but risk goes up is what I'm sayin
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Failboat
Fuck Up

Registered: 02/01/18
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Word, I thought it seemed a bit early. I'll wait till it's thicker.
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Steevo
Just a cog in the death wheel



Registered: 03/02/17
Posts: 2,702
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Re: Making shoeboxes [Re: Failboat]
#25044597 - 03/07/18 06:01 AM (5 years, 10 months ago) |
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Quote:
Quirkmeister92 said:
Quote:
Steevo said: Colonized enough? Either fully colonized or nothing imo. Why would you open a jar or bag of spawn that wasn't 100%

Duh, I was asking for your opinion as to if you consider that 100% or not. So if you would like to share...
Wet grains pushed up against the glass that never fully colonize are a bad sign. Iโd spawn all the jars that look like that to separate shoeboxes and cross my fingers they give at least 1 flush before crapping out. Its hard to get a good look at whatโs going on without really clear pics but thatโs just my opinion and wtf do I know anyway?
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Shrooms4life
TrippyShaman



Registered: 06/08/17
Posts: 1,375
Loc: Under the Viel
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Re: Making shoeboxes [Re: Failboat]
#25044598 - 03/07/18 06:04 AM (5 years, 10 months ago) |
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Here's the pesa clone shoe box this morning, I did like 6 hours of open air fruiting yesterday after work and then flipped the lid for overnight.
 It was spawned 2:2 with a pseudo casing at spawn. Tub was spawned on the 16th of last month.
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JHOVA
Post whore



Registered: 02/17/17
Posts: 4,727
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Shits dry and matted. Not much hope for that
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Humble Newcomer
Diddler de niรฑos



Registered: 03/12/17
Posts: 1,483
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Re: Making shoeboxes [Re: JHOVA]
#25044629 - 03/07/18 06:46 AM (5 years, 10 months ago) |
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Yeah doesn't look great.
Also like mushboy said I wouldn't worry about cloning any of those, much less getting an isolate. It's important to know how all that works but if it were me I'd be excited to go back to MS.
When u see a cluster of fat fruits that are growing tightly togrther you'll get excited and when you're excited and watch a certain section the whole grow, that's when you clone and consider isolation.
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Shrooms4life
TrippyShaman



Registered: 06/08/17
Posts: 1,375
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Re: Making shoeboxes [Re: JHOVA]
#25044639 - 03/07/18 06:57 AM (5 years, 10 months ago) |
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Quote:
JHOVA said: Shits dry and matted. Not much hope for that
Maybe the pic is just shit on here but it's had moisture beads on the surface just like the surface condition thread shows the entire grow.
Trust I'm not trying to make excuses here it's obvious to you guys and myself that I can do better but everytime I checked on this bitch it had plenty of moisture on the surface and sides of the container and my cvg prep was a little over feild cap so I didn't want to over saturate the tub.
But I appreciate the feedback nonetheless, what should I have done if you guys had this situation? Looked misted all the time but I guess is dry and matted?
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Shrooms4life
TrippyShaman



Registered: 06/08/17
Posts: 1,375
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Quote:
Humble Newcomer said: Yeah doesn't look great.
Also like mushboy said I wouldn't worry about cloning any of those, much less getting an isolate. It's important to know how all that works but if it were me I'd be excited to go back to MS.
When u see a cluster of fat fruits that are growing tightly togrther you'll get excited and when you're excited and watch a certain section the whole grow, that's when you clone and consider isolation.
You mean kinda like this? Cause it's the same clone different fc.
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FishLevelMidnight
Aquaman



Registered: 09/01/17
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I think this Redboy is going to explode! Check out that cluster in the middle. Spawned 2-24-18; 1 mycoquart rye spawn to 1.5 quart bulk (1:1:1 coir:verm:black kow with ~7% gypsum).
Look out for a giveaway in a little over a week for any non-purple prints.

I finally stopped trying to be the best and just let it go, only checking a day or so ago. I gave a very light misting to get all of the myc glistening today, but otherwise itโs been cranking with the lid snapped on since spawning.
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Germs
Space Force


Registered: 06/26/11
Posts: 4,607
Loc: Texas
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Quote:
fishermansjc said: I think this Redboy is going to explode! Check out that cluster in the middle. Spawned 2-24-18; 1 mycoquart rye spawn to 1.5 quart bulk (1:1:1 coir:verm:black kow with ~7% gypsum).
Look out for a giveaway in a little over a week for any non-purple prints.

I finally stopped trying to be the best and just let it go, only checking a day or so ago. I gave a very light misting to get all of the myc glistening today, but otherwise itโs been cranking with the lid snapped on since spawning.
Looks great! Redboy pinned heavily for me like that too. Looking forward to seeing the fruits
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