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InvisibleMunchauzen
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Registered: 06/22/11
Posts: 14,342
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Re: Making shoeboxes [Re: Hopeless_Hound]
    #25018197 - 02/24/18 10:35 AM (5 years, 10 months ago)

Quote:

Hopeless_Hound said:
Mush my point is I’m using grain like agar. Agar isn’t necessary because YOU CAN CLEAN CULTURES WITH GRAIN!!!!! It’s actually much less susceptible to mold spore growth than agar is. Please tell me what isn’t possible with my method, I can make transfers, hell I could isolate, clone. Etc. agar is unnecessary and for the work we are doing it. Tell me why do we use agar if you can do the same thing with grain?



This is fundamentally wrong. You cannot clean cultures with grain. You are flat out wrong. Grains contains endospores. Endospores are not killed with a 2 hour 15psi PC cycle. It would require something like a 24 hour 15 psi PC cycle to completely kill endospores. When we PC grains, we are simply damaging the endospores. This is why PC'd jars of grains have a set shelf life and will contaminate if let sit for too long, generally around 2 weeks is when the endospores revive and start to germinate.

Anything that has grains in it has endospores in it. This is why Grain Liquid Culture teks, aka GLC, is among the lowest on the totem pole of inoculation teks. Sure, it will work, sometimes. But there are way better methods out there.

Quote:

actually much less susceptible to mold spore growth than agar



You completely misunderstand the reason we even use agar. We want it to inhibit growth. Nothing hides on agar, thats how we know the culture is clean, because anything and everything will readily grow on it. So a visual inspection of an agar plate is the best indicator you have in mycology that a culture is clean or not. That is simply not possible with grains because the presence of damaged dormant endospores that will remain in them permanently. And as others may know, bacteria writes open invitations for molds.

Check your attitude, it doesn't coincide with your expertise or research in amateur mycology.


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InvisibleHumble Newcomer
Diddler de niños
I'm a teapot


Registered: 03/12/17
Posts: 1,483
Re: Making shoeboxes [Re: Mycolorado]
    #25018206 - 02/24/18 10:39 AM (5 years, 10 months ago)

Way easier to see becauseof growth on a two dimensional plane vs 3dimensional. You can't avoid that, and a possible faster colonization on the plate, which may help cut away from something and ultimately save a culture.

I think everyone here appreciates seeing new approaches, make your own thread but I'd ask you don't state it as fact as better than agar because so many first timers will do anything someone says.

I also think you should eat a slice of humble pie bro, lol, you think you cured cancer and you're challenging everyone to point out things wrong with it and I can see one or two already and I'm a noob.

As shroommancy said, bacteria would concern me. And 2d is huge to me.

Few of us here are scientists. Most of us would like to help another tho. Until people start acting a fool


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How I made my mush GH


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InvisibleShroomymancer
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Registered: 10/20/17
Posts: 485
Re: Making shoeboxes [Re: Shroomymancer]
    #25018210 - 02/24/18 10:41 AM (5 years, 10 months ago)

Back on topic post here. For the past week I said fuck it and threw every lid and dub in a pile. The results were very positive. Increased growth height, speed, and over all yield. So now I am going to attempt an entire run from pins open air.




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beep boop


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OfflineSofaking420
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Registered: 02/09/18
Posts: 1,126
Loc: Myco mountain
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Re: Making shoeboxes [Re: Sofaking420]
    #25018220 - 02/24/18 10:46 AM (5 years, 10 months ago)

Quote:

Sofaking420 said:
So here's some updates pictures of project shoebox. I notice lots of top spider leg looking things. If I understand what I've read this can be too moist or lack of fae. Could any one confirm this? I would appreciate any input. Also included is a pic of fruiting area, again if anyone would be so nice as to share some pointers , links.













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InvisiblemushboyMDiscord
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Re: Making shoeboxes [Re: Sofaking420]
    #25018224 - 02/24/18 10:48 AM (5 years, 10 months ago)

looks decent. fruiting area is ok:shrug: i keep mine in an open closet. doesnt have to be fancy or anything.


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InvisibleHumble Newcomer
Diddler de niños
I'm a teapot


Registered: 03/12/17
Posts: 1,483
Re: Making shoeboxes [Re: Shroomymancer]
    #25018232 - 02/24/18 10:52 AM (5 years, 10 months ago)

Quote:

Shroomymancer said:
For the past week I said fuck it and threw every lid and dub in a pile. The results were very positive.




I wish when I said fuck it and threw stuff in a pile they landed uniform and beautiful like that.


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How I made my mush GH


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InvisibleShroomymancer
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Registered: 10/20/17
Posts: 485
Re: Making shoeboxes [Re: Humble Newcomer]
    #25018236 - 02/24/18 10:56 AM (5 years, 10 months ago)

Quote:

Humble Newcomer said:
Quote:

Shroomymancer said:
For the past week I said fuck it and threw every lid and dub in a pile. The results were very positive.




I wish when I said fuck it and threw stuff in a pile they landed uniform and beautiful like that.




Lol, I meant all the lids and dub tops.


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beep boop


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OfflineSteevo
Just a cog in the death wheel
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Registered: 03/02/17
Posts: 2,702
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Re: Making shoeboxes [Re: Shroomymancer]
    #25018323 - 02/24/18 11:48 AM (5 years, 10 months ago)

I noticed some green in this atl#7 shoebox when I opened the lid yesterday so I went ahead and harvested the stones. It was only in one tiny spot on the casing and everything smelled normal. I'm curious as to how the stones differ with different substrates. The ones I pulled from the wbs spawn before making this shoebox looked and felt different than these which look like small potatoes.
EDIT- My bad, the sub was 50/50+ with sand added



Edited by Steevo (02/24/18 11:53 AM)


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Offlinewushroow
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Registered: 02/03/17
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Re: Making shoeboxes [Re: Shroomymancer]
    #25018324 - 02/24/18 11:48 AM (5 years, 10 months ago)

What are "proper surface conditions" for shoeboxes?


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:tripmolecule:  :thataintright:  :tripmolecule:


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InvisibleJust_A_Noob
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Registered: 12/30/16
Posts: 6,809
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Re: Making shoeboxes [Re: wushroow]
    #25018328 - 02/24/18 11:52 AM (5 years, 10 months ago)

Evaporating water droplets


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Wearing a mask is bad for my physical, emotional, and spiritual health.
Complying = Consent
Wide Mouth 1/2 Pint No-Pour TEK
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InvisibleGerms
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Registered: 06/26/11
Posts: 4,607
Loc: Texas
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Re: Making shoeboxes [Re: Just_A_Noob]
    #25018332 - 02/24/18 11:54 AM (5 years, 10 months ago)

Quote:

Just_A_Noob said:
Evaporating water droplets



Hey noob what’s the cubes in your right sig pic. I love those caps, alot


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OfflineSteevo
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Re: Making shoeboxes [Re: Germs]
    #25018338 - 02/24/18 11:56 AM (5 years, 10 months ago)

Cubes :lol:


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InvisibleGerms
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Re: Making shoeboxes [Re: Steevo]
    #25018342 - 02/24/18 11:56 AM (5 years, 10 months ago)

I walked right into that one... :facepalm: i need sum weed


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InvisibleHumble Newcomer
Diddler de niños
I'm a teapot


Registered: 03/12/17
Posts: 1,483
Re: Making shoeboxes [Re: Germs]
    #25018346 - 02/24/18 11:57 AM (5 years, 10 months ago)

What am I missing about the sclerotia or stones?

A few kinds of mush produce them and fruits, but aren't the fruits more potent?

Stones are sought-after because they're rare and neat and look crazy?


--------------------
How I made my mush GH


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InvisiblemushboyMDiscord
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Re: Making shoeboxes [Re: Humble Newcomer]
    #25018356 - 02/24/18 12:00 PM (5 years, 10 months ago)

some guy i knew(dealer type) was convinced only truffles could be made into chocolates because mushrooms rot in chocolate and ladies like chocolate.:wutermelon:

i saw this guy turn down wild picked shrooms because he wanted to make chocolates with them. what a noob(i later ate those wild shrooms)
the fruits are more potent than cubes. and have a slightly different edge to them. and they are just another species to cultivate.


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InvisibleHumble Newcomer
Diddler de niños
I'm a teapot


Registered: 03/12/17
Posts: 1,483
Re: Making shoeboxes [Re: mushboy]
    #25018366 - 02/24/18 12:02 PM (5 years, 10 months ago)

Some grow both? Think I read PE6 and ATL7, both of those can grow both stones or mushrooms, and the potency is different between the stones and mush?


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How I made my mush GH


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Invisiblethelanzii

Registered: 11/13/12
Posts: 5,434
Re: Making shoeboxes [Re: Humble Newcomer]
    #25018371 - 02/24/18 12:04 PM (5 years, 10 months ago)

vice has a documentary on active sclerotia

stones are sought after from a cultivation pov cause they only need a jar to grow in


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OfflineSteevo
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Re: Making shoeboxes [Re: mushboy]
    #25018372 - 02/24/18 12:05 PM (5 years, 10 months ago)

Stones are dead simple. Set and forget. Mexicana strain produce sclerotia. I can't say whether the stones are more or less potent that the fruits. I haven't had the opportunity to compare yet


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InvisibleHumble Newcomer
Diddler de niños
I'm a teapot


Registered: 03/12/17
Posts: 1,483
Re: Making shoeboxes [Re: Steevo]
    #25018382 - 02/24/18 12:09 PM (5 years, 10 months ago)

So much to grow


--------------------
How I made my mush GH


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InvisibleJust_A_Noob
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Registered: 12/30/16
Posts: 6,809
Loc: PNW
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Re: Making shoeboxes [Re: Germs]
    #25018388 - 02/24/18 12:11 PM (5 years, 10 months ago)

Quote:

Germs said:
Quote:

Just_A_Noob said:
Evaporating water droplets



Hey noob what’s the cubes in your right sig pic. I love those caps, alot




GT





--------------------

Wearing a mask is bad for my physical, emotional, and spiritual health.
Complying = Consent
Wide Mouth 1/2 Pint No-Pour TEK
TC Teks & Links


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Jump to top Pages: < First | < Back | 183 | 184 | 185 | 186 | 187 | 188 | 189 | 190 | 191 | 192 | 193 | 194 | 195 | 196 | 197 | 198 | 199 | 200 | 201 | 202 | 203 | Next > | Last >

Shop: Kraken Kratom Red Vein Kratom   Original Sensible Seeds Autoflowering Cannabis Seeds   North Spore Bulk Substrate   Mushroom-Hut Substrate Bags   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Maeng Da Thai Kratom Leaf Powder


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