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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Re: Making shoeboxes [Re: HighKing]
#24907554 - 01/12/18 09:56 AM (6 years, 18 days ago) |
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Multiple transfers on agar doesn't mean it is clean, IF the eye doesn't catch it contaminations. Some stuff is hard to see on agar and, being new to it, your eye is still untrained.
-------------------- JOIN THE POW WOW
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Shroomymancer
Copy Cat



Registered: 10/20/17
Posts: 485
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Just to add to the contam conversation. I have done nothing but bucket tek since I started in November without issue. I did discover something interesting however. I have a wooden spoon with a long handle that worked really well for stirring up the sub in the bucket. I left this spoon in one of my buckets for two weeks ( I have two so don't always need the one). When I opened the lid the spoon was turning green! Trich was colonizing the wood spoon. However there was nothing in the left over coir/verm/gypsum in the bottom of the bucket itself. That wooden spoon has been in my kitchen for years around all the moldy breads and cheeses etc etc. In my mind, the spoon itself was the vector for contamination and now I am smart enough to shake and rotate my buckets with a lid on (TheMadHatter420's smart idea). Interesting to note that the coir was not growing anything in that bucket tho. I've had 1 shoe box get trich after first flush in 3 months. Sometimes you have to look harder for the contamination problem
-------------------- beep boop
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Xclozure
Stoned as shiiitakes



Registered: 09/09/12
Posts: 421
Last seen: 1 year, 6 months
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mad do you use bucket tek with success?? still a lil iffy, been doing shoeboxes but was gonna do a mono or two. although now that I look at , his shoeboxes are mad bacterial lol just want some confirms with success
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HighKing
Stranger


Registered: 09/14/17
Posts: 38
Last seen: 8 months, 19 days
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Re: Making shoeboxes [Re: Xclozure]
#24907634 - 01/12/18 10:34 AM (6 years, 18 days ago) |
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One thing I would like to ask, so when I was dealing with trich, I was reading a book by Paul Stamets and he said to use hydrogen peroxide diluted with water to combat it....i have still been spraying these tubs with the same solution. Would this contribute to the bacteria?
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Making shoeboxes [Re: HighKing] 1
#24907637 - 01/12/18 10:37 AM (6 years, 18 days ago) |
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Stop doing that right now. I dont know if p-stam said that about trich or cobweb. Either way, you toss contams not treat.
If he indeed said that about trich ill shit myself and laugh at anyone who takes him seriously after that.
Do not ever spray with peroxide. In fact? Throw peroxide out. Its garbage.
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Re: Making shoeboxes [Re: Xclozure]
#24907639 - 01/12/18 10:38 AM (6 years, 18 days ago) |
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Yup I bucket tek.
-------------------- JOIN THE POW WOW
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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http://www.mycomasters.com/Advantages-FAQs.html#FAQs
is this?
Quote:
Peroxide kills mushroom spores, so you can grow agar cultures in the same building you use to fruit your mushrooms, even if the mushrooms produce a high spore load.

What does that even mean??
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Xclozure
Stoned as shiiitakes



Registered: 09/09/12
Posts: 421
Last seen: 1 year, 6 months
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Quote:
TheMadHatter420 said: Yup I bucket tek.
thanks man gonna give it a shot
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Making shoeboxes [Re: mushboy]
#24907660 - 01/12/18 10:45 AM (6 years, 18 days ago) |
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That's a terrible link you posted definitely 
I never realized how much good info is in this thread maybe I'll pop in here more often
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Making shoeboxes [Re: Xclozure]
#24907662 - 01/12/18 10:48 AM (6 years, 18 days ago) |
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Quote:
The spawn can then be grown on a bookshelf in your home, rather than in a sterile laboratory.
Paul reads like someone i know here...
Ok ill stop. I should of posted that link in mushcult gen discussion. Way to derail my own thread
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Hopeless_Hound
Stranger
Registered: 08/21/17
Posts: 343
Last seen: 11 months, 20 days
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Re: Making shoeboxes [Re: mushboy]
#24907681 - 01/12/18 10:58 AM (6 years, 18 days ago) |
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I never understood the whole focuse of 100% lab sterility outside of inoculation. I mean these mushrooms grow from shit... definitely not a sterile environment, why would a lab environment be more productive than a bookshelf? A bookshelf is arguably closer to a field than a laboratory...
-------------------- MS Brazillian shoebox
  AA+ MS shoebox
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HighKing
Stranger


Registered: 09/14/17
Posts: 38
Last seen: 8 months, 19 days
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Re: Making shoeboxes [Re: mushboy]
#24907682 - 01/12/18 10:58 AM (6 years, 18 days ago) |
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Okie dokie then...glad I asked about it
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Making shoeboxes [Re: HighKing]
#24907695 - 01/12/18 11:01 AM (6 years, 18 days ago) |
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Me two.
Dont let questions freak you out. You asked a question that led to me searching for something that turned into comic gold.
I thank you!
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HighKing
Stranger


Registered: 09/14/17
Posts: 38
Last seen: 8 months, 19 days
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Re: Making shoeboxes [Re: mushboy]
#24907797 - 01/12/18 11:42 AM (6 years, 18 days ago) |
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Also went back to the book I was reading and Paul was using the hydrogen peroxide to sterilize the woodchip sub before hand. musta been hella high when I read that, thinking he meant it was for topical use...so my bad
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kanemush
Grumpy

Registered: 12/07/17
Posts: 1,502
Last seen: 8 months, 30 days
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Re: Making shoeboxes [Re: HighKing] 1
#24907871 - 01/12/18 12:12 PM (6 years, 18 days ago) |
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Well this is my 1st try at bulk or coir in a shoebox. I had some brf cakes setting around until my agar is ready, so just seeing what happens.
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PreparationH
apply daily

Registered: 03/28/05
Posts: 18,306
Loc: Amsterdam
Last seen: 9 hours, 48 minutes
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Quote:
Hopeless_Hound said: I never understood the whole focuse of 100% lab sterility outside of inoculation. I mean these mushrooms grow from shit... definitely not a sterile environment, why would a lab environment be more productive than a bookshelf? A bookshelf is arguably closer to a field than a laboratory...
I think it has to do with contaminates taking hold. Outdoors there's constant FAE so contaminates are held at bay. This is why bread grows mold in your home but throw it outside and it never turns green.
At least that's what RR told me I believe.
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ChickenFarmer
Lost Child


Registered: 11/30/17
Posts: 230
Last seen: 5 years, 10 months
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So why couldn't we supply constant FAE?
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Hopeless_Hound
Stranger
Registered: 08/21/17
Posts: 343
Last seen: 11 months, 20 days
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Ahhhh well I pretty much always open air fruit which really seems to make the cubes immune response work better ime.
-------------------- MS Brazillian shoebox
  AA+ MS shoebox
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Quote:
ChickenFarmer said: So why couldn't we supply constant FAE?
Think of it more like air exposure. I hate calling it air exchange 
Anyway, your substrate will dry out too fast. Unless you make a near soaking wet substrate with uber clean spawn and open air it the entire time. I still had to mist and it wasnt worth the hassle fyi.
You can do partial open air(constant fae as you put it) by leaving the lids off while you are home to babysit the conditions, returning the lid while you are away.
Or just leave the lid flipped the entire time.
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OriginalGinger
Lackadaisical



Registered: 07/01/17
Posts: 279
Loc: Cape Town, South Africa
Last seen: 2 years, 2 months
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Re: Making shoeboxes [Re: mushboy]
#24908549 - 01/12/18 05:57 PM (6 years, 18 days ago) |
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Quote:
OriginalGinger said: Never had trich but I've cleaned out cobweb mold containers with just bleach/Handy Andy, then just bleach again, then water. then alcohol rubdown before using again.
I spoke too soon, got my first ever trichoderma tonight, first it looked like huge hyphal knots but as soon as I saw a green tint appearing I tossed it, washed it and took a shower.
Prior to tossing it I wiped my grow area down with bleach with the little air filter running because I had to open my other tubs to mist them since they are fruiting.
Does trich spread spores in very early stages?
-------------------- "On a desperate mission to find a dung lover print!"
Edited by OriginalGinger (01/12/18 05:58 PM)
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