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OfflineJFlint

Registered: 11/12/17
Posts: 115
Last seen: 5 years, 7 months
Re: Making shoeboxes [Re: Shroomymancer]
    #24904911 - 01/11/18 07:21 AM (6 years, 19 days ago)

Quote:

Shroomymancer said:
I'm going to take a guess and say you are over misting which is a common thing to do. I ended up with a couple sb's that had amazing pin sets and nearly wall to wall fruits that were all 3 inches tall. Dense little buggers but hobbits.  I think I was over watering them. In other SB's I held back and tried my best to follow the "set and forget". I keep reading from TC's that if you have made your substrate correctly you shouldn't have to do anything for the entire first flush. I have had larger fruits appearing in these sb's. Also second flush has shown better results on those initial ones as well.  Most of mine are dubbed btw.



Agh. I fear you’re correct. I told myself I was gonna be less zealous about misting this time around. Ive only been misting lightly when it seems to dry up a bit. Maybe three times a day. Four tops. Maybe I’ll leave the ones that are still developing alone more. I hope it isn’t too late. I’ll post some pics later this evening.


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InvisibleMateja
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Registered: 07/14/16
Posts: 7,948
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Re: Making shoeboxes [Re: JFlint]
    #24904916 - 01/11/18 07:24 AM (6 years, 19 days ago)

Wauw that´s a lot of misting. I have practically between zero and 30% RH in the grow room and I get away with misting every third day or every other day :shrug:


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OfflineJFlint

Registered: 11/12/17
Posts: 115
Last seen: 5 years, 7 months
Re: Making shoeboxes [Re: Mateja]
    #24904942 - 01/11/18 07:43 AM (6 years, 19 days ago)

Damn. See I wanted to do that. I left one of my unmodified monos alone like that and it dried the fuck out and totally killed my pinset everywhere except the center of the tub. I’ll keep an eye on the SBs and try to leave them alone.


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InvisibleTheMadHatter420
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Registered: 10/12/16
Posts: 12,941
Re: Making shoeboxes [Re: JFlint] * 1
    #24904952 - 01/11/18 07:52 AM (6 years, 19 days ago)

My opinion is keep a close eye on it from knotting to full pin set. That doesn't mean mist every time, but check on it a couple times a day. I feel this is a critical stage that can make or break the grow. You don't want unnecessary misting, but it will only take a short time of dryness, at this stage, to kill off a portion of the smaller pins. Once the pin set is in, and starting to grow, you don't need to mist, this is when they should really have FAE pushed. Well this is what seem to be working for me. I to still need to slap my own hand on some tubs, and not mist, and yet I have a couple others that could have used an extra misting or 2.


--------------------
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OfflineManifoldPrime
Per Ardua Ad Astra
I'm a teapot


Registered: 03/16/17
Posts: 1,313
Loc: South Africa Flag
Last seen: 1 year, 1 month
Re: Making shoeboxes [Re: TheMadHatter420]
    #24904980 - 01/11/18 08:23 AM (6 years, 19 days ago)

Did 4 boxes at the close of last year and 3 have triched out. That, combined with a recent batch of petris that 50% have trich spots, and I'm getting real tired of this shit.

I'm gonna assume it wasnt the spawn since I shake test usually at 20-40% and at 90-100%. The spawn looked fine, I carefully check it out.

The trich came in at the top first. I did a 1-2cm thich casing of CVG, so maybe this is the problem. My CVG was bucket prepped then pressure cooked for 90min. I'm guessing that maybe sterilising the CVG is not the way to go. So the next batch I do is going to be done 50% PC'd 50% not. other than that, I dont know whats causing this other than higher spore load from having a good few trich breakouts in the past few months. I'm gonna do a scrub down of my labtable and totes anyway.

On a related note, I wanna try salvage the boxes (about 4-6) themselves , I'm gonna scrub with soapy water till theres no visible matter, then bleach bomb the lot (20% bleach and water) for 24 hours, then rescrub, run through the dish and spray with iso after drying. Should be fine afterwards? should I do a dry run with them with just 1 half pint of good spawn and a bit of coir spread out amongst all of them to see if they trich out due to remenant trich spores? stupid idea?

Any help would be appreciated since this hobby is causing more frustration than anything nowadays.


Edited by ManifoldPrime (01/11/18 08:24 AM)


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InvisiblemushboyMDiscord
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Re: Making shoeboxes [Re: ManifoldPrime]
    #24905010 - 01/11/18 08:34 AM (6 years, 19 days ago)

Just a blanket diagnoses here but if your petris are dirty how can you say your spawn 'checks out'?

Maybe if you review/go over your sterile prep ppl can help you out!

If a coco shoebox trichs out your spawn is dirty.  No arguing that fact:shrug:


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OfflineManifoldPrime
Per Ardua Ad Astra
I'm a teapot


Registered: 03/16/17
Posts: 1,313
Loc: South Africa Flag
Last seen: 1 year, 1 month
Re: Making shoeboxes [Re: mushboy]
    #24905021 - 01/11/18 08:44 AM (6 years, 19 days ago)

>If your petris are dirty how can you say your spawn 'checks out'?

The batch of petries in question was made a few days AFTER I bulked the spawn. The spawn was made from EZLC that gets made from clean petries, then tested for 3 generations.

>Maybe if you review/go over your sterile prep ppl can help you out!

I'll record my next sesh a bit.

>If a coco shoebox trichs out your spawn is dirty.  No arguing that fact:shrug:

While I know this is based on yours and other vet's experience, since it started on the very surface, and I did a casing layer, I dont necessarily accept this. If the spawn wasnt even in contact with the infection site, how could this be?

Although I am insecure about my grain prep. I'm getting a better PC since mine is a POS and I only recently figured its not getting right up to 15 PSI.


If there was trich in the spawn, wouldnt the shake test confirm that? why would the spawn only trich out after bulk subbing? When bulk subbing, how much should the spawn be shaken up?


--------------------
My Magnum Opus:Thai Variety Comparison

Please correct me if I say something wrong!

| Current Trade Print Availability:Some PESA and RW available for South African posters. Snail Mail only. |


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OfflineJFlint

Registered: 11/12/17
Posts: 115
Last seen: 5 years, 7 months
Re: Making shoeboxes [Re: TheMadHatter420]
    #24905043 - 01/11/18 08:54 AM (6 years, 19 days ago)

Quote:

TheMadHatter420 said:
My opinion is keep a close eye on it from knotting to full pin set. That doesn't mean mist every time, but check on it a couple times a day. I feel this is a critical stage that can make or break the grow. You don't want unnecessary misting, but it will only take a short time of dryness, at this stage, to kill off a portion of the smaller pins. Once the pin set is in, and starting to grow, you don't need to mist, this is when they should really have FAE pushed. Well this is what seem to be working for me. I to still need to slap my own hand on some tubs, and not mist, and yet I have a couple others that could have used an extra misting or 2.



Awesome to hear your input Madhatter. I always enjoy the amount of detail you add to your posts! I thought I’d heard that misting is not as necessary once pinset is in from other cultivators.


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InvisiblemushboyMDiscord
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Re: Making shoeboxes [Re: JFlint]
    #24905112 - 01/11/18 09:36 AM (6 years, 19 days ago)

Quote:

While I know this is based on yours and other vet's experience, since it started on the very surface, and I did a casing layer, I dont necessarily accept this. If the spawn wasnt even in contact with the infection site, how could this be?




because when you mix your bulk up you are rolling dirty(or clean) grains all over the substrate. take a colonized
grain and just roll it around on agar and remove the grain. that media will show growth all over the place.

get that grain prep down tight. even bacterial cultures put to properly prepped grains shouldnt mold out right away.
id say your main issue is potentially questionable cultures+slightly wonky grains=bad time. a combination of BS:thumbdown:
so tackle each one separately until you move onto the next. as in, get those grains DRY and sterilized 100%

also if you can, make up some simple LC. inoculate with a 'clean' culture by your definition. LCs are unforgiving with
hidden contams. you can use a variety of tools to test your LC on other agar plates. i bought extra long inoc loops for this
purpose but ive also used a sterilized(pced for 15min) metal kabob skewers. anyway the point is, if your culture had any
hidden nasties, it will grow out just like the myc in the LC. test that lc on agar. if its dirty then you can tell yourself
your cultures cleanliness is not up to par. im in the process of sorting that kinda shit out myself.

cultures id used to think were 'good enough to use' are turning out to be dirty.

because my definition of 'clean' is not were it needs to be. back to the books for both of us homie:thumbup:

i also sterilized ALL my coco for about 4months. all that really accomplished was me allowing myself to be lazy
with my sterile technique cause .. 'fuck it the coco is sterilized what am i worried about??' <--noob attitude. guilty as charged.

was that casing coco?


Edited by mushboy (01/11/18 09:42 AM)


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OfflineManifoldPrime
Per Ardua Ad Astra
I'm a teapot


Registered: 03/16/17
Posts: 1,313
Loc: South Africa Flag
Last seen: 1 year, 1 month
Re: Making shoeboxes [Re: mushboy]
    #24905159 - 01/11/18 10:04 AM (6 years, 19 days ago)

Yes, the casing layer was just more sterilised CVG.

Ima take down these notes and get everything prepped for this weekend. clean up, strip down everything, and do more generations of agar before I do more grain work.


--------------------
My Magnum Opus:Thai Variety Comparison

Please correct me if I say something wrong!

| Current Trade Print Availability:Some PESA and RW available for South African posters. Snail Mail only. |


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InvisiblemushboyMDiscord
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Re: Making shoeboxes [Re: ManifoldPrime]
    #24905168 - 01/11/18 10:06 AM (6 years, 19 days ago)

post pics of your cultures too. doing a zillion transfers is pointless if you keep letting bacteria or whatever ride shotgun.

i transfered white mold once for like 13 transfers thinking it would clear up:facepalm:


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OfflineManifoldPrime
Per Ardua Ad Astra
I'm a teapot


Registered: 03/16/17
Posts: 1,313
Loc: South Africa Flag
Last seen: 1 year, 1 month
Re: Making shoeboxes [Re: mushboy]
    #24905175 - 01/11/18 10:09 AM (6 years, 19 days ago)

FYI I got a microscope to check out my cultures and so far I'm satisfied with what I see. I'll post pics this weekend.


--------------------
My Magnum Opus:Thai Variety Comparison

Please correct me if I say something wrong!

| Current Trade Print Availability:Some PESA and RW available for South African posters. Snail Mail only. |


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InvisiblemushboyMDiscord
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Re: Making shoeboxes [Re: ManifoldPrime]
    #24905178 - 01/11/18 10:10 AM (6 years, 19 days ago)

:cool:


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InvisibleJosex
#cheat_code
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Registered: 11/13/15
Posts: 8,995
Loc: Flag
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Re: Making shoeboxes [Re: mushboy]
    #24905181 - 01/11/18 10:10 AM (6 years, 19 days ago)

Quote:

mushboy said:
Quote:

While I know this is based on yours and other vet's experience, since it started on the very surface, and I did a casing layer, I dont necessarily accept this. If the spawn wasnt even in contact with the infection site, how could this be?




because when you mix your bulk up you are rolling dirty(or clean) grains all over the substrate. take a colonized
grain and just roll it around on agar and remove the grain. that media will show growth all over the place.

get that grain prep down tight. even bacterial cultures put to properly prepped grains shouldnt mold out right away.
id say your main issue is potentially questionable cultures+slightly wonky grains=bad time. a combination of BS:thumbdown:
so tackle each one separately until you move onto the next. as in, get those grains DRY and sterilized 100%

also if you can, make up some simple LC. inoculate with a 'clean' culture by your definition. LCs are unforgiving with
hidden contams. you can use a variety of tools to test your LC on other agar plates. i bought extra long inoc loops for this
purpose but ive also used a sterilized(pced for 15min) metal kabob skewers. anyway the point is, if your culture had any
hidden nasties, it will grow out just like the myc in the LC. test that lc on agar. if its dirty then you can tell yourself
your cultures cleanliness is not up to par. im in the process of sorting that kinda shit out myself.

cultures id used to think were 'good enough to use' are turning out to be dirty.

because my definition of 'clean' is not were it needs to be. back to the books for both of us homie:thumbup:

i also sterilized ALL my coco for about 4months. all that really accomplished was me allowing myself to be lazy
with my sterile technique cause .. 'fuck it the coco is sterilized what am i worried about??' <--noob attitude. guilty as charged.

was that casing coco?



:kaneclap:


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Invisiblestareatclouds
star eat clouds?
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Registered: 09/29/14
Posts: 9,887
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Re: Making shoeboxes [Re: Mateja]
    #24905194 - 01/11/18 10:15 AM (6 years, 19 days ago)

Quote:

Mateah said:
and once again seems I´ll get fruits from bacterial spawn :shrug:




:facepalm:


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InvisibleJHOVA
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Registered: 02/17/17
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Re: Making shoeboxes [Re: stareatclouds]
    #24905209 - 01/11/18 10:21 AM (6 years, 19 days ago)

Clean spawn is a journey. It’s not one thing and I see it taking many people a while to get. I don’t see any shame in admitting it and I don’t think he’s proud of dirty spawn probably quite the opposite.


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Invisiblevan hattonFacebookDiscord
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Registered: 11/23/14
Posts: 5,617
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Re: Making shoeboxes [Re: JHOVA]
    #24905215 - 01/11/18 10:23 AM (6 years, 19 days ago)

Fuck I'm proud of bacterial spawn as long as it doesn't mold before first.


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


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InvisiblemushboyMDiscord
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Re: Making shoeboxes [Re: JHOVA]
    #24905216 - 01/11/18 10:24 AM (6 years, 19 days ago)

Quote:

JHOVA said:
Clean spawn is a journey.




its like making music. just keep at it. the same parts. over and over and over again. keep practicing. you are always practicing.

anyone who says they dont have time/dont need to improve is either a fool or a lair. or a TC with a M tag:tongue:


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Invisiblevan hattonFacebookDiscord
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Registered: 11/23/14
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Re: Making shoeboxes [Re: mushboy]
    #24905226 - 01/11/18 10:26 AM (6 years, 19 days ago)

:cookiemonster:


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


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OfflineJFlint

Registered: 11/12/17
Posts: 115
Last seen: 5 years, 7 months
Re: Making shoeboxes [Re: van hatton] * 1
    #24905279 - 01/11/18 10:43 AM (6 years, 19 days ago)

Here’s the best tub I got going so far. On my lunch break today I went home, opened the dubtub a bit ajar, and used all my will (maybe exaggerating a bit), not to mist it. I think these will spring up at least a little bit more before opening up. Fingers crossed.

Also, if I’m refraining from misting as much as possible, do I still want moisture beads, or a glistening surface while the fruits mature?



Think I may clone that pointy one in the back left. I like that a lot for some reason.


Edited by JFlint (01/11/18 10:45 AM)


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