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OfflineSundar
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Registered: 12/15/16
Posts: 12
Loc: Quito - Ecuador
Last seen: 10 months, 21 days
Contamination in petri dishes
    #23975836 - 01/02/17 11:33 AM (7 years, 1 month ago)

Hi people,

Just came back from holidays and a week earlier I did some transfers to petri dishes. I posted in an earlier post that since I'm from Ecuador I don't have spore syringes available for purchase so I have to start growing from samples taken from the outdoors. The growing medium was made by mixing brown sugar, water from boiled potatoes and gelatin since I have no agar.

:smile: all of the dishes are contaminated but nonetheless I managed to get some good pictures. So let me share you the pictures and discuss about the possible rescue methods


Penicillium


I have no idea who this guy is


black mold growing on top of Penicillium


Rhizopus growing along a white mushroom (could be the intended Psilocybe)


In this plate something interesting happened. This plate was inyected with the spores from a psilocybe. The interesting thing is that penicillium and the other contaminants did not dissolve the gelatin medium, but there is something that did. Could it be the psilocybe? Bacteria? In the case that is the psilocybe that means its liquid culture and The next transfer must be done from this liquid?


Another plate inoculated with spores. Same thing, but with black clusters of something (I would like to know this one since is the one taking over my bathroom)



Same liquid



This plate was inoculated with blue Oyster mushroom. It also dissolved the gelatin and this strange film appeared (could it be the Oyster?)


And this is the only one that has neither black or green contamination. Aside from the yellow spot this plate is fairly clean. This plate is actually the second transfer from a previous contaminated plate.

Now then. I have some questions I'd be very grateful if someone has any information about them.

-Why and who dissolved the gelatin?
-What are those black clusters in the plate I mentioned above? Rhizopus? It is very aggressive

Since I must cultivate from these I hope the psilocybe is living in the digested-gelatin-liquid to transfer it. I just found a place where they sell agar-agar (It's a noodle like presentation in a envelope with Chinese characters) and I will make my next plates with this. Any advice is greatly appreciated.

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OfflineMattisfat
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Registered: 01/20/16
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Re: Contamination in petri dishes [Re: Sundar]
    #23975912 - 01/02/17 11:58 AM (7 years, 1 month ago)

i say dont bother with the liquid just take as many samples as possible from the one that looks like mycelium, take the samples from the edge furthest away from any contams


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OfflineTiamo
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Re: Contamination in petri dishes [Re: Mattisfat]
    #23978409 - 01/03/17 10:38 AM (7 years, 1 month ago)

Gelatin is not a substitute for agar agar. It will break down fairly quickly, contaminate and give you all kinds of issues. You can easily order agar agar from eBay on the cheap. Here you go, it's 50 grams of agar agar for under 4 USD, free WW shipping.


--------------------


If you have used a Miraculix Psilocybin QTest, could you please share your results?

Shipping free Ps. natalensis spore prints to any address in The Netherlands, just :pm:

:mushroom2: Mush love :mushroom2:

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OfflineSundar
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Registered: 12/15/16
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Loc: Quito - Ecuador
Last seen: 10 months, 21 days
Re: Contamination in petri dishes [Re: Tiamo]
    #23981081 - 01/04/17 10:58 AM (7 years, 1 month ago)

I'm ordering it right now!!!

thank you

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OfflineRolledUhhp
Amateur Cultivator

Registered: 08/10/16
Posts: 246
Last seen: 3 years, 7 months
Re: Contamination in petri dishes [Re: Sundar]
    #23982346 - 01/04/17 07:32 PM (7 years, 1 month ago)

I'm going to have to get a few ounces of agar at that price!

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OfflinePsilocyBen17
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Re: Contamination in petri dishes [Re: RolledUhhp]
    #23986037 - 01/06/17 08:58 AM (7 years, 1 month ago)

Hey OP, just wanted to say kudos for being here. I admire that you are cloning from wild fruits for your first grow. Good for you for jumping right in. I've given you a rating. PM me if you need some help in the future.


--------------------
PsilocyBen's Trade List!

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Offlinehombre
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Registered: 07/07/16
Posts: 127
Last seen: 4 years, 4 months
Re: Contamination in petri dishes [Re: PsilocyBen17]
    #23986079 - 01/06/17 09:21 AM (7 years, 1 month ago)

I don’t want to offend you, but your plates look pretty horrible. Do you use a still air box ? Anyways, don’t let me discourage you and keep up the work.

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OfflinePsilocyBen17
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Registered: 10/20/13
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Re: Contamination in petri dishes [Re: hombre]
    #23986093 - 01/06/17 09:32 AM (7 years, 1 month ago)

Did you read the OP? He used gelatin, which explains why his plates don't look the greatest. Im confident things will clear up when he gets the proper supplies.


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OfflineSundar
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Re: Contamination in petri dishes [Re: Sundar]
    #24035243 - 01/24/17 06:38 AM (7 years, 1 month ago)

Hi again, thank you people for your kind support. I'm getting very exited with this process.

I found agar-agar, built a still-air box and now the plates look much better, I already have a plate growing pure white, contamination free, nice and thready Psilocybe Caerulescens mycelium. Thank you so much for the information.

But since this post is about contamination, let me continue with some recent pictures:

In order to preserve a blue oyster mushroom log that already is quite old I putted some pins in petri dishes.

This one amazes me. The pin matured and opened to drop spores (I assume, since those gooey white lines are in the pattern of the gills above). So, thats the first question. Are those lines germinated spores? they look gooey and not like mycelium but I don't know if they would eventually develop to mycelium. Nevertheless I transfered those spores to other plates in order to protect them from contamination around in the same plate. What do you think? If they are spores that would mean I'm actually growing a new generation, non-senescence oyster mushroom?







This next one is quite strange. It's a transfer from a Psilocybe Caerulescens.

The growth is radial but those bumps baffle me. Is that contamination o sclerotia? I think it must be contamination since I have other plates with pure mycelium, but the question still remains. I would like to know how to recognize a sclerotia.







This plate is quite decent in that the transfer is growing pretty pure. the only contaminant is that goo at the edge of the plate. It might have landed there while I was covering it with micropore tape (I have no parafilm). Do you recognize that goo? Is it bacteria?








This transfer is from a shitake bought from the supermarket. It just looks odd. the agar near the growth is becoming brown. I'm letting this one to continue to grow to see more clearly.







And to finish with, just a horrible contaminated pinhead.


Thank you very much again for your support

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OfflineTiamo
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Re: Contamination in petri dishes [Re: Sundar]
    #24035250 - 01/24/17 06:46 AM (7 years, 1 month ago)

Those are some good photos. I'm hesitant to identify anything going on; try posting in mushroom cultivation in stead of here, you'll get more answers and it would apply better there imo.


--------------------


If you have used a Miraculix Psilocybin QTest, could you please share your results?

Shipping free Ps. natalensis spore prints to any address in The Netherlands, just :pm:

:mushroom2: Mush love :mushroom2:

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OfflineRolledUhhp
Amateur Cultivator

Registered: 08/10/16
Posts: 246
Last seen: 3 years, 7 months
Re: Contamination in petri dishes [Re: Tiamo]
    #24036053 - 01/24/17 01:31 PM (7 years, 1 month ago)

I don't think it would be sclerotia, perhaps its primordia?

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