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OfflineRoostorf
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Syringe driven recirculating inoculation system
    #24033485 - 01/23/17 03:09 PM (7 years, 7 days ago)

This idea just popped into my head after thinking about this place in Asia that I heard inoculates liquid culture directly to bulk.  I'm not sure where I read it or if its true, but the system I have imagined would allow jar of liquid culture to screw or plug into the loop of check valves and be recirculated via a large plunger or syringe.  I would assume sterilization could be archived by running steam or bleach or alcohol through the system and I would think colonization would occur rapidly.  I don't know, I'm really new to this, maybe once I get more set up to to even the basics I can play around with something like this?

I drew this like an idiot on my finger on a small tablet and its sideways as well.  Grain or bulk in the main chamber and I would guess it could be done in large pp5 Tupperwares or even something larger like a 55 gallon drum. 

Btw if you take a simple syringe and hooked it to a motor it will make a water pump, that could come in handy....



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Learning something new everyday

Would love to trade medicinal and edible vultures and spores


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OfflineRoostorf
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Re: Syringe driven recirculating inoculation system [Re: Roostorf]
    #24033501 - 01/23/17 03:13 PM (7 years, 7 days ago)



This is what I meant by the water pump, good thing I didn't have to draw again


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Learning something new everyday

Would love to trade medicinal and edible vultures and spores


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OfflineDactylium
Don't touch me I'm sterile


Registered: 08/12/16
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Re: Syringe driven recirculating inoculation system [Re: Roostorf]
    #24033504 - 01/23/17 03:13 PM (7 years, 7 days ago)

:joneswtf:
Having a hard time wrapping my head around this one


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Invisiblewildernessjunkie
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Re: Syringe driven recirculating inoculation system [Re: Roostorf]
    #24033510 - 01/23/17 03:16 PM (7 years, 7 days ago)

https://www.mycosupply.com/cgi-bin/shopper.cgi?preadd=action&key=SSIG01

MycoSupply has Inoculation guns. Sounds like what you are thinking of.


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OfflineDactylium
Don't touch me I'm sterile


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Re: Syringe driven recirculating inoculation system [Re: wildernessjunkie]
    #24033519 - 01/23/17 03:18 PM (7 years, 7 days ago)

Whoa that's pretty rad


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InvisiblebodhisattaMDiscordReddit
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Re: Syringe driven recirculating inoculation system [Re: Dactylium]
    #24033533 - 01/23/17 03:24 PM (7 years, 7 days ago)

I think understanding how fungi works will shed a lot of light onto why this idea makes no fucking sense

you could make a bioreactor and just feed the LC more nutrients and seperate off the colonized portion regularly.

we have 0 need for more spores after the first spores start to grow, there needs to be no syringe in the setup at all

also bleach and alcohol are sanitizers they don't sterilize anything


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OfflineRoostorf
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Re: Syringe driven recirculating inoculation system [Re: bodhisatta]
    #24033585 - 01/23/17 03:48 PM (7 years, 7 days ago)

I wasn't saying anywhere to add more spores, the syringe is just the drives the system and can be made into an water pump that can also be pressure sterilized.  I was trying to figure out a way to basically submerge the substrate or grain with recirculating liquid culture for a day in a sterile system, without introducing contaminants.  Just for a few hours or maybe a day, then it could be unplugged from the loop and colonize super quick from everywhere at once. 

Anyways I was smoking a bunch of weed when I thought of it, so I may not have explained it well and it might not make any fucking sense either


--------------------
Learning something new everyday

Would love to trade medicinal and edible vultures and spores


Edited by Roostorf (01/23/17 03:52 PM)


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InvisiblebodhisattaMDiscordReddit
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Re: Syringe driven recirculating inoculation system [Re: Roostorf]
    #24033594 - 01/23/17 03:53 PM (7 years, 7 days ago)

You want a carlsberg flask.


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