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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Quote:
bodhisatta said: https://www.shroomery.org/forums/showflat.php/Number/21922023
Scroll down to cloning and strain isolation
included that whole workman quote you just posted, as a link, and the link to where he said it.
https://www.shroomery.org/forums/showflat.php/Number/10242476#10242476
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Tookitooki
Mycological Fabricator



Registered: 07/28/16
Posts: 1,157
Loc: Nowhere
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My eyes failed me. I scrolled right past it. My apologies bod.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Jk, haha. No problem.
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KauaiOrca
Waterman

Registered: 08/12/08
Posts: 3,131
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Thanks for posting that link and the post. That's the one I was referring to. Lots in there to digest. A lot of it over my rather casual hobbiest head but it confirms two things I've learned from simple trial and error.
1) Multispore grows that come from a strong clone culture tend to perform very well, at least in the grows I do. 2) The multispore grows from generation to generation tend to perform more and more consistently from generation to generation and, at least for the first 2-3 generations, get better and better probably because the spores are coming from good fruits that seem to like those growing conditions.
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,378
Loc: The Inexpressible...
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As already stated, cloning is a short-cut to grab the desired genetic traits u are looking for. Very similar to cloning plants by taking a branch and growing roots out of it. It keeps the genetic traits of the "mother" and its specific DNA info.
The more broad, longer-term method is isolating genetics, which as stated, is (for example) taking one spore print from one desired fruit, growing from that one spore print, taking another fruit for that grow and repeating that process till u get the desired results u want. Artifical selection is the proper term.
Cloning is also artifical selection, but is the short-cut method for mushrooms.
Cloning is like that but one step: taking a fruit and cloning it for a culture, then using the culture to grow more fruits like that fruit u cloned.
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RealityCzech
Entropy enthusiast


Registered: 12/29/16
Posts: 90
Last seen: 6 years, 3 months
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If you are interested, as I am, in increasing genetic variation to work towards new stable lineages with some interesting phenotypes (I posted earlier about this), you're looking at mixing up multiple lineages. I have made syringes using spores from multiple different lineages with very little success... it looks like the first spores to germinate tend to dominate and overrun the others, so one lineage reigns supreme.
My current attempts at getting a monokaryon off an agar plate has resulted in 2 promising specimens transferred to their own plates off of 15 plates that were streaked. Unfortunately, both of these came from the same original plate, so they are both from the same spore print. That and, although both samples germinated as a small single location and are growing thin and wispy, I cannot really be sure at this time that these really are monokaryons.
It turns out that, without the random factors present in a natural environment where spores are sparsely distributed from a number of different lineages, it's actually fairly difficult to get highly domesticated lineages to cross. Once I can get a number of crosses and some good genetic variation I can start working on looking for and selecting traits. Unfortunately, along the way, I foresee having to stabilize a few different lineages and cross them again to get where I want to be.
I'm just mentioning this because you should understand that these stable lineages are really quite stable. There is a lot of unknown in multispore, but if you use older stabilized lineages, you really do have a pretty good chance of success, although not guaranteed.
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,378
Loc: The Inexpressible...
Last seen: 46 seconds
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crossing two domesticated strains, for example a GT and Mexican, would be very difficult.
U would need one single spore from one strain and one single spore from the other strain and mate them. Very difficult.
But with enough trial and error, i bet u could mate two spores like that.
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AK1000
Stranger



Registered: 06/06/16
Posts: 488
Last seen: 2 years, 5 months
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How do you mate microscopic spores like that?
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,378
Loc: The Inexpressible...
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Re: Why clone? [Re: AK1000]
#24034610 - 01/23/17 09:30 PM (7 years, 7 days ago) |
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With a microscope, im guessing. Ive never attempted it.
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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (06/21/18 11:22 AM)
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RealityCzech
Entropy enthusiast


Registered: 12/29/16
Posts: 90
Last seen: 6 years, 3 months
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Yeah... it is proving to be ridiculously difficult. I guess most satisfying endeavors are. We'll see if it is worth the frustration in the end.
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,378
Loc: The Inexpressible...
Last seen: 46 seconds
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Quote:
amidogen said: Check out workman's journal. He documents how he created the APE variety. Also Pastywhyte's journal for his RustyWhyte variety. Fascinating reads 
I read a little about Roger Rabbit's Red Boy strain mating using snake vemon. Shit had me like
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