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seventy7
not quite 78


Registered: 01/10/17
Posts: 62
Last seen: 6 years, 10 months
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A beginner's first grow log - seventy7 goes for the monotub! *DELETED*
#24030373 - 01/22/17 12:13 PM (7 years, 8 days ago) |
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Post deleted by seventy7Reason for deletion: g2g
Edited by seventy7 (01/30/17 11:27 AM)
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MortySmith



Registered: 03/28/16
Posts: 68
Last seen: 6 years, 4 months
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Re: A beginner's first grow log - seventy7 goes for the monotub! [Re: seventy7]
#24030417 - 01/22/17 12:35 PM (7 years, 8 days ago) |
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I was about to make the "No agar?!?!" comment but I see you mentioned it yourself lol, its good that you know now! The thing that sucks, IMO, is that if you aren't using agar then you will never truly know where you went wrong.
Could it have been improper prep/sterile technique? Maybe, but could it also have been a contam in your LC? Yes, or even a contam in your original spore syringe! You really never know where the contam entered the picture if you aren't using agar first, that is how I see it at least...
The point is, you know about agar now. So stop doing grains and LC right now, whip up some pasty plates and weed out any contams through transfers. Then you will have some 100% clean mycelium ready to inoculate LC, grains, etc.
Another opinion of mine, just formed recently as I was looking into LC now that I have agar down, is that LC is *not* worth it, even after agar. Look into greain-to-grain transfers, all the upsides of LC with none of the downsides. That is what I have decided to go with instead of LC after some research. EDIT: I just saw the bit at the bottom where you mentioned this. Looks like you are on the right track!
Edited by MortySmith (01/22/17 12:38 PM)
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: A beginner's first grow log - seventy7 goes for the monotub! [Re: seventy7]
#24030423 - 01/22/17 12:36 PM (7 years, 8 days ago) |
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looks like you put a lot of effort into this. the LC is terrifying me to be honest.
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seventy7
not quite 78


Registered: 01/10/17
Posts: 62
Last seen: 6 years, 10 months
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Re: A beginner's first grow log - seventy7 goes for the monotub! *DELETED* [Re: mushboy]
#24030623 - 01/22/17 01:51 PM (7 years, 8 days ago) |
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Post deleted by seventy7Reason for deletion: g2g
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MortySmith



Registered: 03/28/16
Posts: 68
Last seen: 6 years, 4 months
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Re: A beginner's first grow log - seventy7 goes for the monotub! [Re: seventy7]
#24032127 - 01/22/17 10:39 PM (7 years, 8 days ago) |
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Yeah man that is what agar is for, I think anyways. Catching all your mistakes so they don't end up in spawn or substrate! I messed up many plates on my agar journey so far lol. The amount of time and effort lost on a some plates is nothing compared to losing entire batches of jars or a whole tub, especially considering you usually end up with usable plates in the end even if you screw up several times along the way with agar.
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seventy7
not quite 78


Registered: 01/10/17
Posts: 62
Last seen: 6 years, 10 months
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Re: A beginner's first grow log - seventy7 goes for the monotub! *DELETED* [Re: MortySmith]
#24033020 - 01/23/17 11:19 AM (7 years, 7 days ago) |
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themadextractor
Stranger


Registered: 06/25/14
Posts: 230
Last seen: 1 year, 3 months
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Re: A beginner's first grow log - seventy7 goes for the monotub! [Re: seventy7]
#24033422 - 01/23/17 02:41 PM (7 years, 7 days ago) |
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I don't think the agar is too far off. You may be able to get a cleanish transfer from that first pictured plate and clean it up more from there. Also if agar is giving you trouble you can do grain on a small level to get clean growth and transfer grains to agar. I use pasty plate dishes like yours half full of grain for germinating syringes. Don't let one failure get you down no matter how big it was. You've already learned where you went wrong. Now you learn how to do it right.
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seventy7
not quite 78


Registered: 01/10/17
Posts: 62
Last seen: 6 years, 10 months
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Re: A beginner's first grow log - seventy7 goes for the monotub! *DELETED* [Re: themadextractor]
#24035804 - 01/24/17 11:26 AM (7 years, 6 days ago) |
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Post deleted by seventy7Reason for deletion: g2g
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MortySmith



Registered: 03/28/16
Posts: 68
Last seen: 6 years, 4 months
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Re: A beginner's first grow log - seventy7 goes for the monotub! [Re: seventy7]
#24035855 - 01/24/17 11:54 AM (7 years, 6 days ago) |
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How do you inoculate your plates? With spore syringes?
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SpicyWizard



Registered: 12/14/15
Posts: 511
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Re: A beginner's first grow log - seventy7 goes for the monotub! [Re: MortySmith]
#24035920 - 01/24/17 12:24 PM (7 years, 6 days ago) |
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seems so. or just rubbed the print all around.
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seventy7
not quite 78


Registered: 01/10/17
Posts: 62
Last seen: 6 years, 10 months
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Re: A beginner's first grow log - seventy7 goes for the monotub! *DELETED* [Re: MortySmith]
#24036056 - 01/24/17 01:34 PM (7 years, 6 days ago) |
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Edited by seventy7 (01/24/17 01:34 PM)
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seventy7
not quite 78


Registered: 01/10/17
Posts: 62
Last seen: 6 years, 10 months
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Re: A beginner's first grow log - seventy7 goes for the monotub! *DELETED* [Re: seventy7]
#24041051 - 01/26/17 11:44 AM (7 years, 4 days ago) |
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seventy7
not quite 78


Registered: 01/10/17
Posts: 62
Last seen: 6 years, 10 months
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Re: A beginner's first grow log - seventy7 goes for the monotub! *DELETED* [Re: seventy7]
#24044226 - 01/27/17 03:31 PM (7 years, 3 days ago) |
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: A beginner's first grow log - seventy7 goes for the monotub! [Re: seventy7]
#24044421 - 01/27/17 04:15 PM (7 years, 3 days ago) |
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As I saw your pictures, I thought "Wow, that is a high spawn:bulk ratio". 2:1 should look quite different, but maybe you just didn't mix it will.
You could, but don't have to, give it a thin pseudo-casing layer of CVG, if you worry about the rye on the surface.
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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