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OfflineTheCyndicate
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Need another set of eyes (UPDATE 1/25/17) transfers in original post.
    #24030035 - 01/22/17 09:47 AM (7 years, 8 days ago)

So I inoculated some plates 2 weeks ago this Monday with MS syringe of a random cube. At first the growth in this one plate look way too fluffy to be mycelium. But the more it grows out with no visible sporulation present and it not looking bacterial. Has me at a cross road. Here are some pics of the plate in question.


1/23/17
After much discussion I transfered some sections of the dish in question and I'm happy with the results so far it's only been about  22 hours since the transfers but they are showing good signs of growth and great characteristics of cube mycelium. Here are some pics of just a few of the transfers. I will post more as they come along.



1/25/17
Like I promised I'm updating this post, (a) to keep a log for my self and (B) to keep anyone that's wants to fallow along informed. (C) to ask any questions I might have when I need to ask them.
Always remember we never stop learning in this hobby. And asking questions is always good:thumbup:

Here are some of the best looking transfers so far.


I just love the way this plate is turning out.


Another angle.



And just think I almost tossed the original plate thinking it's was contaminated. Thanks for all the words of wisdom everyone.

I'm wondering if I should take more transfers tomorrow, or let the mycelium Network grow out more so it's easier to identify sectors for isolation.
Till the next update ! 



Edited by TheCyndicate (01/25/17 06:09 PM)


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Offlineenlightenment
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Re: Need another set of eyes [Re: TheCyndicate]
    #24030164 - 01/22/17 10:36 AM (7 years, 8 days ago)

IMO it's white aspergillus or another unwanted fungus. It's possible that it does not change the color. That makes it very difficult to differentiate it from cubensis mycelium.



The discoloration of the agar is a bad sign. I don't see discoloration on your plate but anyway I think it's mold.

What agar recipe did you use?


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InvisiblebodhisattaMDiscordReddit
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Re: Need another set of eyes [Re: enlightenment]
    #24030188 - 01/22/17 10:47 AM (7 years, 8 days ago)

Quote:

enlightenment said:
IMO it's white aspergillus or another unwanted fungus. It's possible that it does not change the color. That makes it very difficult to differentiate it from cubensis mycelium.



The discoloration of the agar is a bad sign. I don't see discoloration on your plate but anyway I think it's mold.

What agar recipe did you use?



Looks a bit too organized to me but also looks funny.


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OfflineKenetic
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Re: Need another set of eyes [Re: bodhisatta]
    #24030235 - 01/22/17 11:05 AM (7 years, 8 days ago)

Being on the edge of the dish is suspicious to me


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OfflineTheCyndicate
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Re: Need another set of eyes [Re: enlightenment]
    #24030273 - 01/22/17 11:33 AM (7 years, 8 days ago)

Quote:

enlightenment said:
IMO it's white aspergillus or another unwanted fungus. It's possible that it does not change the color. That makes it very difficult to differentiate it from cubensis mycelium.



The discoloration of the agar is a bad sign. I don't see discoloration on your plate but anyway I think it's mold.

What agar recipe did you use?




20.0 g Potato Flakes
10.0 g Dextrose
15.0 g Agar
1.0 L Demineralized Water

Potato Dextrose Agar and Potato Flake Agar are formulations developed to promote sporulation of fungi. The potatoes contained in these media provide nutritious bases for luxuriant growth of fungi. Both media contain dextrose as a growth stimulant


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OfflineTheCyndicate
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Re: Need another set of eyes [Re: bodhisatta]
    #24030277 - 01/22/17 11:34 AM (7 years, 8 days ago)

It looks organized . But it looks strange totally agree.


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OfflineTheCyndicate
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Re: Need another set of eyes [Re: Kenetic]
    #24030281 - 01/22/17 11:36 AM (7 years, 8 days ago)

To me as well. I inoculated in the center of the dish but then again the drop of spore solution could have spread around while I was applying parafilm.


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Re: Need another set of eyes [Re: TheCyndicate]
    #24030298 - 01/22/17 11:45 AM (7 years, 8 days ago)

Just looks like weak cube mycelium to me.


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OfflineTheCyndicate
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Re: Need another set of eyes [Re: Pastywhyte]
    #24030307 - 01/22/17 11:50 AM (7 years, 8 days ago)

Quote:

Pastywhyte said:
Just looks like weak cube mycelium to me.




Sup pasty! My brain tells me the same thing. But my paranoia says otherwise. I will take a few transfers and see what grows out. Like I said it's been almost 2 weeks and no sporulation and the growth seems to be consistent and uniform. I got another batch of agar in the PC right now so I will do transfers this evening.


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Offlineenlightenment
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Re: Need another set of eyes [Re: Kenetic]
    #24030313 - 01/22/17 11:52 AM (7 years, 8 days ago)

It looks like. But I really doubt it is cube mycelium. I could be wrong, though.
Don't toss this plate it would be interesting how it develops.

Quote:

kenetic said:
Being on the edge of the dish is suspicious to me



True. I have seen a lot of contams on petri dishes that started from the edges.


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OfflineTheCyndicate
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Re: Need another set of eyes [Re: enlightenment]
    #24030316 - 01/22/17 11:54 AM (7 years, 8 days ago)

Quote:

enlightenment said:
It looks like. But I really doubt it is cube mycelium. I could be wrong, though.
Don't toss this plate it would be interesting how it develops.

Quote:

kenetic said:
Being on the edge of the dish is suspicious to me



True. I have seen a lot of contams on petri dishes that started from the edges.



I won't toss it. Like I said to pasty, I might do some transfers this evening.


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Re: Need another set of eyes [Re: enlightenment]
    #24030319 - 01/22/17 11:55 AM (7 years, 8 days ago)

How's it going cyndicate? I'm not really gonna say it is or isn't mold or cubes at this point. I would like to see what a transfer looks like.


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OfflineTheCyndicate
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Re: Need another set of eyes [Re: Pastywhyte]
    #24030335 - 01/22/17 12:00 PM (7 years, 8 days ago)

Quote:

Pastywhyte said:
How's it going cyndicate? I'm not really gonna say it is or isn't mold or cubes at this point. I would like to see what a transfer looks like.




It's going good man. Been away form the community for too long. Miss you guys. Like seeing everyone has their TC tags proud of y'all!

Yea I'm gonna transfer tonight and see what happens. I'm trying to use really old spore syringes like 6 years old..Lol. it's been a struggle. The only thing I have in long storage is a master slant of atl#7.


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InvisiblemushboyMDiscord
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Re: Need another set of eyes [Re: TheCyndicate]
    #24030344 - 01/22/17 12:04 PM (7 years, 8 days ago)

ive had many pastyplates look like that. its myc 100% imo. when it grows up on the side ive noticed
that the myc will grow on the plastic itself. can bacteria do that?


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OfflineTheCyndicate
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Re: Need another set of eyes [Re: mushboy]
    #24030371 - 01/22/17 12:12 PM (7 years, 8 days ago)

Quote:

mushboy said:
ive had many pastyplates look like that. its myc 100% imo. when it grows up on the side ive noticed
that the myc will grow on the plastic itself. can bacteria do that?




Yes bacteria can do that. My gut tells me it's mycelium. But then again, it's been at least a year since I have even touched this hobby. So I'm overly paranoid.


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Re: Need another set of eyes [Re: TheCyndicate]
    #24032079 - 01/22/17 10:13 PM (7 years, 7 days ago)

Of things Ive inoculated Ive only see myc eat through weird things Lol but question for you guys can you take a piece on a slide look at it and be able to tell if its cube or something else?


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Re: Need another set of eyes [Re: spore-ty]
    #24032087 - 01/22/17 10:16 PM (7 years, 7 days ago)

Yes.


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Re: Need another set of eyes [Re: bodhisatta]
    #24032088 - 01/22/17 10:17 PM (7 years, 7 days ago)

Quote:

bodhisatta said:
Yes.





Would 1200x be sufficient ?


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InvisibleAdden
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Re: Need another set of eyes [Re: spore-ty]
    #24032092 - 01/22/17 10:18 PM (7 years, 7 days ago)

Mycelium that does that on cubensis and other species (not varieties) are usually contaminated. Filamentus mycelium looks a lot different especially since your plate looks like it has waves to it. I get them when cleaning up wild prints. It's some kind of bacteria and it makes the mycelium look like it's sending out two "waves" but myc doesn't do that. I'd transfer like pasty said but also make some new plates.


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OfflineTheCyndicate
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Re: Need another set of eyes [Re: Adden]
    #24032503 - 01/23/17 04:56 AM (7 years, 7 days ago)

Quote:

Adden said:
Mycelium that does that on cubensis and other species (not varieties) are usually contaminated. Filamentus mycelium looks a lot different especially since your plate looks like it has waves to it. I get them when cleaning up wild prints. It's some kind of bacteria and it makes the mycelium look like it's sending out two "waves" but myc doesn't do that. I'd transfer like pasty said but also make some new plates.




Yeah I have done both, I have made transfers, and inoculated new plates with MS, but instead of just dropping spore soulition to the dishes I sterilized some swabs and saturated the swabs with MS and draged them asross 20 plates.


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Offlineenlightenment
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Re: Need another set of eyes [Re: TheCyndicate]
    #24032537 - 01/23/17 05:35 AM (7 years, 7 days ago)

I checked the pictures again. The growth started exactly from the spot where the parafilm does not fit tight. Is there another layer of parafilm that is not visible on the picture?


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OfflineTheCyndicate
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Re: Need another set of eyes [Re: enlightenment]
    #24032564 - 01/23/17 06:01 AM (7 years, 7 days ago)

Quote:

enlightenment said:
I checked the pictures again. The growth started exactly from the spot where the parafilm does not fit tight. Is there another layer of parafilm that is not visible on the picture?




Honestly I can't remember. I will check when I get home this afternoon. But I wanna say yes their is another layer of parafilm I always double wrap my dishes.


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Re: Need another set of eyes [Re: spore-ty]
    #24032717 - 01/23/17 08:38 AM (7 years, 7 days ago)

Quote:

spore-ty said:
Quote:

bodhisatta said:
Yes.





Would 1200x be sufficient ?



400is. 1000x requires oil 1200x doesn't really exist its a gimmick


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Re: Need another set of eyes [Re: bodhisatta]
    #24032761 - 01/23/17 09:05 AM (7 years, 7 days ago)

hey cyn! :hi: great to see you back man, hope you stick around a little longer this time :wink:

Looks like cube myc to me too, not sure I like why it starts right at the edge of the dish tho unless you just threw some spores on a plate.


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Re: Need another set of eyes [Re: spacechildo]
    #24032833 - 01/23/17 09:41 AM (7 years, 7 days ago)

Yep I think it's cube myc too. I've also had cubes grow from the edge when using spore syringes. Fuckin water can go everywhere.


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Re: Need another set of eyes [Re: Mad Season]
    #24032836 - 01/23/17 09:42 AM (7 years, 7 days ago)

Streak plates FTW


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Re: Need another set of eyes [Re: bodhisatta]
    #24032839 - 01/23/17 09:43 AM (7 years, 7 days ago)

Yep streaking is the way to avoid this shit


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OfflineTheCyndicate
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Re: Need another set of eyes [Re: Mad Season]
    #24033010 - 01/23/17 11:14 AM (7 years, 7 days ago)

Quote:

Mad Season said:
Yep streaking is the way to avoid this shit




I don't know why it never dawned on me , but yes this is the way to avoid this bull shit.


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Re: Need another set of eyes [Re: TheCyndicate]
    #24033088 - 01/23/17 12:08 PM (7 years, 7 days ago)

I updated my agar Tek to include streak plate technique


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OfflineTheCyndicate
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Re: Need another set of eyes [Re: bodhisatta]
    #24033216 - 01/23/17 01:12 PM (7 years, 7 days ago)

Quote:

bodhisatta said:
I updated my agar Tek to include streak plate technique



Good shit bodhisatta. I will check it out.


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Re: Need another set of eyes [Re: bodhisatta]
    #24033497 - 01/23/17 03:12 PM (7 years, 7 days ago)

Quote:

bodhisatta said:
I updated my agar Tek to include streak plate technique






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Re: Need another set of eyes [Re: spore-ty]
    #24033611 - 01/23/17 04:01 PM (7 years, 7 days ago)

Good stuff Cyn

Those plates you made will be perfect is my guess


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OfflineTheCyndicate
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Re: Need another set of eyes [Re: bodhisatta]
    #24033629 - 01/23/17 04:09 PM (7 years, 7 days ago)

Yea man that's what I'm thinking. I like my plates like I like my pussy PINK!!


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OfflineTheCyndicate
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Re: Need another set of eyes [Re: TheCyndicate]
    #24039445 - 01/25/17 06:13 PM (7 years, 5 days ago)

:bump: update in original post.


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