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InvisibleMycolorado
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Re: Chicon Nindo and tampanensis [Re: Edmunter] * 1
    #23968954 - 12/30/16 07:18 PM (7 years, 2 months ago)

looks like the first stones are gonna start here. This is a tamp jar...the same type growth led to stones on agar. 


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Re: Chicon Nindo and tampanensis [Re: Mycolorado] * 1
    #23970238 - 12/31/16 09:23 AM (7 years, 2 months ago)

Here's a new stone forming on a stone transfer plate.  This is the plate with the small bacteria colony (visible in the second pic as a clear spot).  The circled dark spot is the new stone.  As I posited on ed's thread, I think bacteria may be the key to triggering or is at least, very influential on stone formation.  Could also just be the physical obstruction on the surface of the agar allowing for an anchor point from which the stone can grow.


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Edited by Mycolorado (12/31/16 10:25 AM)

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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #23972747 - 01/01/17 11:21 AM (7 years, 1 month ago)



Sorry I didn't try for a better picture yet, but here's my tamp plates.
From right to left -> rightmost plate: inoc'd on 11-21 with a MS syringe; 2nd & 3rd from right: transfers from original plate; 4th & 5th from right: each with a slice of one of a stone from the first transfer plate.

this is my first experience with sclerotia producing stuffs, I really didn't do anything special to them that I know of. I had no idea they don't typically grow stones right on the plate D=


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Edited by Aneres (01/01/17 11:22 AM)

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InvisibleMycolorado
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Re: Chicon Nindo and tampanensis [Re: Aneres]
    #23973636 - 01/01/17 05:37 PM (7 years, 1 month ago)

Nice!  So you had a transfer plate produce more stones?  I' debated slicing them but decided against it as I had several to use.  Looks like a pretty beefy stone in the second from the right!


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InvisibleAneres
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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #23973731 - 01/01/17 06:00 PM (7 years, 1 month ago)

Quote:

Mycolorado said:
Nice!  So you had a transfer plate produce more stones?  I' debated slicing them but decided against it as I had several to use.  Looks like a pretty beefy stone in the second from the right!




Actually the transfers grew stones long before the original did. I very honestly had (have, heh) no idea how exactly one is supposed to be working with these, I admit i've just been winging it and treating them the same as the rest of my cultures. It was just a random choice really, to cut the stone (into three pieces, one of which remained in situ). I like to do two of everything if possible in case of contams, so the two leftmost plates in that pic are from the same stone.

One thing to note which I don't know if is significant or not - the three rightmost plates were originally dyed green with food coloring but the myc turned it back to a very pale amber


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Re: Chicon Nindo and tampanensis [Re: Aneres]
    #23979793 - 01/03/17 07:44 PM (7 years, 1 month ago)

Progress pic of the first stone with a second starting.  Not crazy explosive growth but still impressive considering the jar is only a third-ish colonized.


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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #23982332 - 01/04/17 07:25 PM (7 years, 1 month ago)

:rockon:


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If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922



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Re: Chicon Nindo and tampanensis [Re: wtfcrazymofo]
    #23982338 - 01/04/17 07:28 PM (7 years, 1 month ago)

that istan vat izz up
That is just how daddy likes it


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InvisibleAneres
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Re: Chicon Nindo and tampanensis [Re: Kenetic]
    #24000281 - 01/11/17 05:45 AM (7 years, 1 month ago)

It suddenly occurs to me that perhaps the reason I got stones when others don't may be because my tamp plates were started on like 12-9 and thus overgrown


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Re: Chicon Nindo and tampanensis [Re: Aneres]
    #24005061 - 01/12/17 06:17 PM (7 years, 1 month ago)

Yeah, it was 3-4 weeks for the CN stone and around 5 weeks for the tamp stones to start on agar...may see the same pattern in the jars.  These are right around 4 weeks and spent the first two at about 69F, so moved pretty slowly.  Anyway, the 4 on the left were shaken.  3 CN (left) and 3 tamps.  The far right jar is the one with the small stone on the bottom.  All the tamp jars look like they may be getting ready to start stoning.



This jar came from the nicest tamp culture and is starting to produce a lot of that dark brown mycelium.  Hope that's an indicator that it's gonna do...something?  Different angles of same jar:



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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #24011794 - 01/15/17 10:27 AM (7 years, 1 month ago)

Tamp stone...super slow growth. 



Here's a shaken CN jar.  Some small stones forming.



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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #24019795 - 01/18/17 12:03 PM (7 years, 1 month ago)

CN stone plate...appears that the stone has grown a new lobe...hard to tell from the pic and all the metabolites covering it.  Also, it's producing a new stone at 1 o'clock.



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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #24028951 - 01/21/17 06:58 PM (7 years, 1 month ago)

CN stones forming in all 3 shaken jars.  They have more of a grey color than the tamps which are more yellow.  I'm going to use the above stone plate to make a LI for a couple jars tomorrow.  Hopefully the isolation and additional water will help them move quicker and stone up better.  Side note, I'm using wheat so not sure if that's effecting stone formation.  I'll run rye on the next round. 



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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #24028964 - 01/21/17 07:05 PM (7 years, 1 month ago)



--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922



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Re: Chicon Nindo and tampanensis [Re: wtfcrazymofo]
    #24028990 - 01/21/17 07:20 PM (7 years, 1 month ago)

Thanks, wtf!  stonesun's shit rocks.  I'm not totally sold on the coffee but will give it a shot.  I think the LI is really going to help.


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stonesun
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Offlinewtfcrazymofo
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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #24029016 - 01/21/17 07:39 PM (7 years, 1 month ago)

The best smell of any grain I've prepped.


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922



Edited by wtfcrazymofo (01/21/17 07:41 PM)

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InvisibleMycolorado
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Re: Chicon Nindo and tampanensis [Re: wtfcrazymofo]
    #24031717 - 01/22/17 07:51 PM (7 years, 1 month ago)

Made 100ml of LI from the CN stone plate and inoculated 4 qts. of rye. May be stretching it.  I transferred the stone which is growing and forming more stones.  Here are a couple different angles. Sorry for the fuzzy second pic.



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InvisiblebodhisattaMDiscordReddit
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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #24031880 - 01/22/17 08:39 PM (7 years, 1 month ago)

:hi:
nice stuff
surprised I didn't notice this thread floating around

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Re: Chicon Nindo and tampanensis [Re: bodhisatta]
    #24031972 - 01/22/17 09:12 PM (7 years, 1 month ago)

Thanks, bodh!


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stonesun
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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
    #24043701 - 01/27/17 12:47 PM (7 years, 1 month ago)

This jar received the majority of LI and is just starting to recover.  Looks like it'll take right off.  Yays LI!



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