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OfflineMagicc
Stranger
Registered: 01/21/17
Posts: 7
Last seen: 7 years, 6 days
First post, first grow attempt. Pretty sure all my jars are contaminated, please verify :(
    #24028920 - 01/21/17 06:47 PM (7 years, 9 days ago)

Long time reader, first time poster :smile:

ok guys im pretty sure i tanked my attempt at my first 4 jars ever, I just want to upload the photos to double check, and maybe to identify the contam, and what the source might be. I didnt use a glove box, but i cleaned the shit out of an already very clean small bathroom, used gloves, clean clothes, mask, 90 minutes of PC at 15 psi, but i think my error was closing the box and putting it in a (disinfected) trash bag, and tying it up. I know that sounds stupid to me now, but its what i seen in the tek i followed, and it worked well for them.

Learn me guys :frown:

Jar 1









Jar 2









jar 3





jar 4



Pretty dissapointed in myself :frown: please learn me ur ways. Mouths shut, and ears are open guys. Thanks in advance.

BTW, there is some writing in white/silverish marker on the outside of some of them in case your wondering what that is.


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Offlinecubensis333
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Registered: 12/28/16
Posts: 222
Last seen: 6 years, 7 months
Re: First post, first grow attempt. Pretty sure all my jars are contaminated, please verify :( [Re: Magicc]
    #24028939 - 01/21/17 06:54 PM (7 years, 9 days ago)

the green mold is Trichoderma


--------------------


Edited by cubensis333 (01/21/17 06:55 PM)


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OfflineMagicc
Stranger
Registered: 01/21/17
Posts: 7
Last seen: 7 years, 6 days
Re: First post, first grow attempt. Pretty sure all my jars are contaminated, please verify :( [Re: cubensis333]
    #24028946 - 01/21/17 06:56 PM (7 years, 9 days ago)

There is no green mould i think thats just the colors from the bathroom affecting the photo. Its all white irl.

Do you have the time to give me a little bit more detailed/specific answer, im dying here, so disapointed :'(


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OnlineLogicaL ChaosM
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Re: First post, first grow attempt. Pretty sure all my jars are contaminated, please verify :( [Re: Magicc] * 1
    #24028952 - 01/21/17 06:59 PM (7 years, 9 days ago)

They look pretty bad.

how did u prep the seeds?


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Invisiblerocknard
I'm a teapot


Registered: 04/05/16
Posts: 20
Re: First post, first grow attempt. Pretty sure all my jars are contaminated, please verify :( [Re: Magicc]
    #24028960 - 01/21/17 07:04 PM (7 years, 9 days ago)

looks pretty gray and wispy in there, maybe some kind of pin mold, did you use a mask/gloves/sab in a sterilized environment?


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OfflineDactylium
Don't touch me I'm sterile


Registered: 08/12/16
Posts: 696
Last seen: 10 months, 13 days
Re: First post, first grow attempt. Pretty sure all my jars are contaminated, please verify :( [Re: Magicc]
    #24028972 - 01/21/17 07:07 PM (7 years, 9 days ago)

Your mistake was using a spore syringe in an open air environment. You'll need to build a still air box and clean your spores up on agar.

Check this post out


Edited by Dactylium (01/21/17 07:10 PM)


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OfflineMagicc
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Registered: 01/21/17
Posts: 7
Last seen: 7 years, 6 days
Re: First post, first grow attempt. Pretty sure all my jars are contaminated, please verify :( [Re: rocknard]
    #24028980 - 01/21/17 07:11 PM (7 years, 9 days ago)

Yeah i explained what i did in my post if you read up.

I prepped the seeds in a pressure cooker, and used a method that didnt include soak+simmering them, just filled the jars with 1 to 2.5 ish ratio of water to seeds. soo 300ml of seeds and 125ml of water if i remember correctly. Not those exact measurements but thats the ratio. Then we pressure cooked it like that and the seeds seemed perfect when they came out, but now after sitting they seem a little over saturated.

Are there any good sites preferably in canada that sell pre sterilized bags with self healing nocc holes or whatever, like the rubber ones that are on medical vials.

cheers, thanks for replying so fast guys


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OfflineMagicc
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Registered: 01/21/17
Posts: 7
Last seen: 7 years, 6 days
Re: First post, first grow attempt. Pretty sure all my jars are contaminated, please verify :( [Re: Dactylium]
    #24028985 - 01/21/17 07:13 PM (7 years, 9 days ago)

Ive seen and heard lots of people knocking up in open air, just after intense sterilization before hand, and having lots of success.

Is it possible to buy pre sterilized substrate ready for knocking up? or could it not be done since it would require it to have moisture content and therefor would get mucked in the mail???

This would be a life saver for me


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OfflineDactylium
Don't touch me I'm sterile


Registered: 08/12/16
Posts: 696
Last seen: 10 months, 13 days
Re: First post, first grow attempt. Pretty sure all my jars are contaminated, please verify :( [Re: Magicc]
    #24028991 - 01/21/17 07:21 PM (7 years, 9 days ago)

You can't sterilize your room my dude. Sanitizers only kill what's already alive, they'll never get rid of spores. Furthermore, there's a very high probability there's bacteria and mold spores inside your spore syringe.

It doesn't make any sense to do all that work prepping a room when you could buy a plastic tote for $10 and put some holes in it.

You're going to experience a very high failure rate and weak yields if you keep inoculating in open air with spore syringes. Pre sterilized subs are also a bad idea for the reasons you stated and more. We pressure cook our grains to provide a window of opportunity for the mycelium to colonize. If left long enough mold spores will repair themselves and germinate. They're like freakin' Majin Buu.


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OfflineMagicc
Stranger
Registered: 01/21/17
Posts: 7
Last seen: 7 years, 6 days
Re: First post, first grow attempt. Pretty sure all my jars are contaminated, please verify :( [Re: Dactylium]
    #24029036 - 01/21/17 07:56 PM (7 years, 9 days ago)

Believe me im not arguing with ya, im just clarifying here, but i sterilized a very small bathroom, wiped down virutally every surface, and sprayed heavily, would that still have a very high failure rate? I am going to make a glove box next time, just want to clarify.


Also, i bought my spore syringe from spores101 so im not worried about that being the problem.

I have 4ml left, is that enough for 4-8 jars?


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OfflineMagicc
Stranger
Registered: 01/21/17
Posts: 7
Last seen: 7 years, 6 days
Re: First post, first grow attempt. Pretty sure all my jars are contaminated, please verify :( [Re: rocknard]
    #24029039 - 01/21/17 07:59 PM (7 years, 9 days ago)

Could really use your input on a few things..

Is it most likely the fact i knocked em up in open air? Even though i sterilized the shit out of a very small bathroom, used disinfected gloves, a mask, etc.

Or could it be because i put the jars in an airtight box and tied it up in a garbage bag?

Also, what are your thoughts on the mushbox pre sterilized rye grain bags, with self healing injection ports?


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Offlinewtfcrazymofo
foil hater
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Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
Last seen: 13 hours, 39 minutes
Re: First post, first grow attempt. Pretty sure all my jars are contaminated, please verify :( [Re: Dactylium]
    #24029041 - 01/21/17 08:00 PM (7 years, 9 days ago)

All you need is foo mans wbs prep, A sab, a flame, good patience, Then maybe some good agar work.

Don't buy pre sterilized grain.

You only sanitized your bathroom.

Nice ears.

Those are some good videos.


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




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InvisiblemushboyMDiscord
modboy
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Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: First post, first grow attempt. Pretty sure all my jars are contaminated, please verify :( [Re: Magicc]
    #24029050 - 01/21/17 08:05 PM (7 years, 9 days ago)

OP jars look like this...


not trich but its some green mold that sounds like asparagus(i was told)

bathrooms are the worse place for anything mushroom related. forget that room is there.
best room is one with the least draft possible...that isnt a moldy basement/bathroom.


please link whatever tek you followed. anything saying to put jars in a trashbag has got to be old as fuck


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OfflineDactylium
Don't touch me I'm sterile


Registered: 08/12/16
Posts: 696
Last seen: 10 months, 13 days
Re: First post, first grow attempt. Pretty sure all my jars are contaminated, please verify :( [Re: Magicc]
    #24029084 - 01/21/17 08:21 PM (7 years, 9 days ago)

Please delete the name of the vendor from your post. It's against the rules.

Spore syringes are always going to be dirty, no matter how reputable the source.

Leaving your jars to colonize in a garbage bag isn't harmful. But ambient light in a room is beneficial for colonization, so don't bother with that.

Try germinating your spores on agar first. It'll take you a little more time but your success rate will be much higher. All of the finest cultivators here do it for a reason. Videos on this are in the link I provided above. Pastywhyte's Easy Agar Tek is also a good read. Lots of new cultivators have success following this tek.


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OfflineWeavieWonder
Time Travel Sucks
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Registered: 08/18/16
Posts: 1,455
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Re: First post, first grow attempt. Pretty sure all my jars are contaminated, please verify :( [Re: mushboy]
    #24029092 - 01/21/17 08:28 PM (7 years, 9 days ago)

Those jars are straight fucked.  Sorry.  Your grain preparation is bunk and your sterile practices are not good.  Keep reading.  Do more research before investing more time and resources.  Surely you have a better place to do clean work than in a bathroom that is infested with mold and shit particles?


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Offlinewtfcrazymofo
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Registered: 07/26/15
Posts: 1,201
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Re: First post, first grow attempt. Pretty sure all my jars are contaminated, please verify :( [Re: WeavieWonder]
    #24029111 - 01/21/17 08:38 PM (7 years, 9 days ago)

If you get sab work down a still air bathroom is not a problem.

Your bunk grain prep is the main problem.  If you had nice moist grain jars, it would have taken longer to see the contams from your poor sterile tec. if any

Clean up that mess, read up, hopefully you are watching those vids, and try again.


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




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InvisiblemushboyMDiscord
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Posts: 32,281
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Re: First post, first grow attempt. Pretty sure all my jars are contaminated, please verify :( [Re: Magicc]
    #24029117 - 01/21/17 08:41 PM (7 years, 9 days ago)

Quote:

Magicc said:
Ive seen and heard lots of people knocking up in open air, just after intense sterilization before hand, and having lots of success.




they lied. intense sterilization? its either sterile or its not:confused:


Quote:

Is it possible to buy pre sterilized substrate ready for knocking up? or could it not be done since it would require it to have moisture content and therefor would get mucked in the mail???...This would be a life saver for me




for FULL proof grain prep just do wild birdseed soaked 24hrs. PCed 2hrs.

ordering premade stuff is a huge waste of money. if you can make pasta, jello, or rice then you can make your own shit for pennies. you fears are well founded about mucked up in the mail.


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OfflineRealityCzech
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Registered: 12/29/16
Posts: 90
Last seen: 6 years, 3 months
Re: First post, first grow attempt. Pretty sure all my jars are contaminated, please verify :( [Re: mushboy]
    #24029137 - 01/21/17 08:58 PM (7 years, 9 days ago)

Quote:

mushboy said:
not trich but its some green mold that sounds like asparagus




Are you thinking of aspergillus?


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InvisiblemushboyMDiscord
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Re: First post, first grow attempt. Pretty sure all my jars are contaminated, please verify :( [Re: RealityCzech]
    #24029139 - 01/21/17 08:58 PM (7 years, 9 days ago)

:thumbup:

part of the 'Trichocomaceae' family... :confused::shrug:


better pic


Edited by mushboy (01/21/17 09:06 PM)


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Invisiblepheasant327

Registered: 10/04/16
Posts: 133
Re: First post, first grow attempt. Pretty sure all my jars are contaminated, please verify :( [Re: mushboy]
    #24029172 - 01/21/17 09:17 PM (7 years, 9 days ago)

I wouldn't bother buying pre-sterilized grain bags, especially if you're going to be using a spore syringe. If you want to use grains or WBS, take the time to learn agar before messing with it. If you're dead set on inoculating straight from a spore syringe just stick with the basics and follow the PF-tek to the letter until you have a good handle on it. (PF tek done right)


--------------------
 


Edited by pheasant327 (01/21/17 09:19 PM)


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