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Offlinemr.psuedo
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Registered: 01/07/17
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metabolites or no?
    #24023800 - 01/19/17 06:54 PM (7 years, 11 days ago)

hey guys, I'm not sure what I'm dealing with here. I've exhausted all other resources before resorting to posting this thread. All help is very much appreciated.



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OfflineHelmetHeads
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Re: metabolites or no? [Re: mr.psuedo]
    #24023812 - 01/19/17 07:01 PM (7 years, 11 days ago)

Looks like colonies of something, bacteria or possibly yeast. Definitely looks like metabolites though.


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Keep your finger out of the jars bro. :shocked: -- Tmethyl


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Offlinemr.psuedo
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Re: metabolites or no? [Re: HelmetHeads]
    #24023817 - 01/19/17 07:02 PM (7 years, 11 days ago)

is it no good? I currently have it separated from the others on a paper towel in the cabinet lol


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OfflineHelmetHeads
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Re: metabolites or no? [Re: mr.psuedo]
    #24023823 - 01/19/17 07:03 PM (7 years, 11 days ago)

Throw it away. You literally touched the cake. Your cake is dead even if it previously was not. Beyond any redemption. It is kill.

Next time, NEVER expose the cake to the outside environment before birthing except for when you do the injection. Even air exposure can contaminate it and your hand will ABSOLUTELY contaminate the cake.


--------------------
Keep your finger out of the jars bro. :shocked: -- Tmethyl


Edited by HelmetHeads (01/19/17 07:08 PM)


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Offlinemr.psuedo
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Re: metabolites or no? [Re: HelmetHeads]
    #24023828 - 01/19/17 07:04 PM (7 years, 11 days ago)

touching it doesn't hurt a cake....

it is fully colonized


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OfflineHelmetHeads
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Re: metabolites or no? [Re: mr.psuedo]
    #24023833 - 01/19/17 07:06 PM (7 years, 11 days ago)

No, it is colonized but not FULLY colonized. A fully colonized cake is white all over, without any substrate let for the mycelium to expand.

edit: I am stoopid. Did not realize it was a rolled cake.


Edited by HelmetHeads (01/19/17 07:11 PM)


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Offlinemr.psuedo
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Re: metabolites or no? [Re: HelmetHeads]
    #24023859 - 01/19/17 07:13 PM (7 years, 11 days ago)

lol its straight. yeah, that shit popped up after I put in the FC. Keeping it on deathrow for a day or two unless further knowledge prohibits otherwise.


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InvisibleMad Season
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Re: metabolites or no? [Re: mr.psuedo]
    #24024900 - 01/20/17 07:49 AM (7 years, 10 days ago)

It's slime mold, probably fuligo, toss.

Potential causes: it was there from the beginning (unlikely IMO), there is bacteria in there that weakened the mycelium enough for it to germinate (MUCH more likely).

Possible causes of bacteria: endospores survived the sterilizarion cycle, they were in the syringe, or you introduced them during inoculation.

Hope this helps.


--------------------
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Offlinemr.psuedo
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Re: metabolites or no? [Re: Mad Season]
    #24033386 - 01/23/17 02:21 PM (7 years, 7 days ago)

Quote:

Mad Season said:
It's slime mold, probably fuligo, toss.

Potential causes: it was there from the beginning (unlikely IMO), there is bacteria in there that weakened the mycelium enough for it to germinate (MUCH more likely).

Possible causes of bacteria: endospores survived the sterilizarion cycle, they were in the syringe, or you introduced them during inoculation.

Hope this helps.




I never saw this, sorry! Thanks for taking the time and bringing this to my attention. Its in the garbage now!


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InvisibleMad Season
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Re: metabolites or no? [Re: mr.psuedo]
    #24034105 - 01/23/17 06:40 PM (7 years, 7 days ago)

Quote:

mr.psuedo said:
Quote:

Mad Season said:
It's slime mold, probably fuligo, toss.

Potential causes: it was there from the beginning (unlikely IMO), there is bacteria in there that weakened the mycelium enough for it to germinate (MUCH more likely).

Possible causes of bacteria: endospores survived the sterilizarion cycle, they were in the syringe, or you introduced them during inoculation.

Hope this helps.




I never saw this, sorry! Thanks for taking the time and bringing this to my attention. Its in the garbage now!



Haha I was wondering why there was no response. Yeah it happens to me too. Shit seems to say you've read it when you haven't sometimes


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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