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MorePies
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Culture fridge froze
#24020977 - 01/18/17 06:47 PM (7 years, 12 days ago) |
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Went to my spawn/culture fridge and the thermostat got knocked, so it froze everything. Is there any way to check if all my grain spawn is still viable other than inoculating bags?
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wildernessjunkie
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Re: Culture fridge froze [Re: MorePies]
#24021003 - 01/18/17 06:54 PM (7 years, 12 days ago) |
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Give it a good shake. If it recovers....
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Forrester
aspiring sociopath


Registered: 02/05/13
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That, or take a single grain and put it on agar.
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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MorePies
Stranger
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Re: Culture fridge froze [Re: Forrester]
#24021201 - 01/18/17 07:54 PM (7 years, 12 days ago) |
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Quote:
wildernessjunkie said: Give it a good shake. If it recovers....
Will do. Spawn heavy if it does?
Quote:
Forrester said: That, or take a single grain and put it on agar.
Got that going too.
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Forrester
aspiring sociopath


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Re: Culture fridge froze [Re: MorePies]
#24021236 - 01/18/17 08:05 PM (7 years, 12 days ago) |
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Quote:
MorePies said:
Quote:
wildernessjunkie said: Give it a good shake. If it recovers....
Will do. Spawn heavy if it does?
What would be the reasoning for that?
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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Stupendous-Yappi
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Re: Culture fridge froze [Re: Forrester]
#24021595 - 01/18/17 10:34 PM (7 years, 12 days ago) |
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If the grain spawn recovers no need to spawn heavy. I put some Nameko spawn in the freezer for over 6 month and it recovered quickly. So, some types won't have any problem with a brief freeze.
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Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
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Quote:
Stupendous-Yappi said: If the grain spawn recovers no need to spawn heavy. I put some Nameko spawn in the freezer for over 6 month and it recovered quickly. So, some types won't have any problem with a brief freeze.

It does depend a lot on species.
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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Gr0wer
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Re: Culture fridge froze [Re: Forrester]
#24021632 - 01/18/17 10:48 PM (7 years, 12 days ago) |
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this is why i do water cultures for backup thats not refrigerated.
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MorePies
Stranger
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Re: Culture fridge froze [Re: Gr0wer]
#24022950 - 01/19/17 01:35 PM (7 years, 11 days ago) |
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Quote:
Gr0wer said:
this is why i do water cultures for backup thats not refrigerated.
How's that working out for you? Any issues on the revive?
Here's hoping. I'll let ya'll know what makes it back from the brink.
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poponon
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Re: Culture fridge froze [Re: MorePies]
#24025035 - 01/20/17 08:43 AM (7 years, 10 days ago) |
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Quote:
MorePies said:
Quote:
Gr0wer said:
this is why i do water cultures for backup thats not refrigerated.
How's that working out for you? Any issues on the revive?
Here's hoping. I'll let ya'll know what makes it back from the brink.
I'm also interested in hearing more about water backup cultures. Is there a tek?
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MorePies
Stranger
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Re: Culture fridge froze [Re: poponon]
#24025274 - 01/20/17 10:52 AM (7 years, 10 days ago) |
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Quote:
poponon said:
I'm also interested in hearing more about water backup cultures. Is there a tek?
Cut up colonized agar and put in sterilized distilled water. Cover air tight and put it on the shelf. I couldn't link you to any, but there are documented cases of this lasting for 10 years.
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poponon
Quaaaack!!!



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Re: Culture fridge froze [Re: MorePies]
#24026372 - 01/20/17 05:42 PM (7 years, 10 days ago) |
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Quote:
MorePies said:
Cut up colonized agar and put in sterilized distilled water. Cover air tight and put it on the shelf. I couldn't link you to any, but there are documented cases of this lasting for 10 years.
thanks for the info. will be trying this out soon
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Forrester
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Re: Culture fridge froze [Re: poponon]
#24026935 - 01/20/17 10:19 PM (7 years, 10 days ago) |
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Yeah just do a search, there's lots of threads on it
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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MorePies
Stranger
Registered: 02/09/16
Posts: 808
Loc: TX
Last seen: 1 month, 3 days
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Re: Culture fridge froze [Re: Forrester]
#24027700 - 01/21/17 09:36 AM (7 years, 9 days ago) |
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So far the king Oyster, Lions mane, a cold blue strain, and block poplar have all recovered!
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