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dr_bizzarro
Stranger


Registered: 09/10/15
Posts: 28
Last seen: 4 years, 12 days
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Shook jars of WBS after innoc. Am I screwed? Re-innoc?
#24020790 - 01/18/17 05:43 PM (7 years, 12 days ago) |
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Heyo!
Regarding Franks WBS Tek. 2nd attempt. ("Aborts" last time)
Somehow I got the idea that shaking jars after inoculation is good, as it disperses spores or some such. After doing this, I read that in fact it's not good at all. (Only, Shake right after sterilizing and at 30% colonization, correct?)
I'm 10 days out with no signs of myco at all.
I'm patient, and can wait, but I don't want to wait for nothing.
Should I just chill? I had the weird idea to innoc them AGAIN and then not shake. Does anyone ever do that? Using single layer of tyvek, then polyfill through lid.
They are in open room with indirect dim light, natural FAE (no fans) and at about 70-77 degrees F.
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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Re: Shook jars of WBS after innoc. Am I screwed? Re-innoc? [Re: dr_bizzarro]
#24020823 - 01/18/17 05:53 PM (7 years, 12 days ago) |
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Do not re-inoculate. Thats asking for trouble.
I would just get more jars and make a 2nd batch. Multiple grows staggered 1 week apart is a great way to alleviate the grow anxiety
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Dactylium
Don't touch me I'm sterile


Registered: 08/12/16
Posts: 696
Last seen: 10 months, 13 days
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Re: Shook jars of WBS after innoc. Am I screwed? Re-innoc? [Re: dr_bizzarro]
#24020863 - 01/18/17 06:15 PM (7 years, 12 days ago) |
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Spores can take weeks to germinate. You should be pleased nothing nasty grew considering you used a spore syringe on grain. If the spores do decide to germinate you're likely in the clear as far as contams go.
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dr_bizzarro
Stranger


Registered: 09/10/15
Posts: 28
Last seen: 4 years, 12 days
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Re: Shook jars of WBS after innoc. Am I screwed? Re-innoc? [Re: Dactylium]
#24020934 - 01/18/17 06:34 PM (7 years, 12 days ago) |
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Quote:
Do not re-inoculate. Thats asking for trouble.
I would just get more jars and make a 2nd batch. Multiple grows staggered 1 week apart is a great way to alleviate the grow anxiety
Ok thanks. Yeah, not a bad idea.
Quote:
You should be pleased nothing nasty grew considering you used a spore syringe on grain. If the spores do decide to germinate you're likely in the clear as far as contams go.
This is the first I've heard about not using spore syringes on WBS. (But I am new, bear with me..) Is there a consensus on that? What's the alternative? I flame and alcohol sterilized of course.
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Dactylium
Don't touch me I'm sterile


Registered: 08/12/16
Posts: 696
Last seen: 10 months, 13 days
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Re: Shook jars of WBS after innoc. Am I screwed? Re-innoc? [Re: dr_bizzarro]
#24020966 - 01/18/17 06:43 PM (7 years, 12 days ago) |
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Spores are collected in open air environments so bacteria or mold spores could have found their way into the syringe. We germinate our spore solution on a petri dish so we can see the contaminants and isolate the mushroom mycelium from them. There's lots of good teks on here and they aren't very difficult to follow. Agar as easy as 1-2-3 is a great place to start.
You use a still air box right?
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Dactylium
Don't touch me I'm sterile


Registered: 08/12/16
Posts: 696
Last seen: 10 months, 13 days
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Re: Shook jars of WBS after innoc. Am I screwed? Re-innoc? [Re: Dactylium]
#24020967 - 01/18/17 06:44 PM (7 years, 12 days ago) |
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Everything you need is in Munch's signature if you're interested.
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: Shook jars of WBS after innoc. Am I screwed? Re-innoc? [Re: dr_bizzarro]
#24020975 - 01/18/17 06:46 PM (7 years, 12 days ago) |
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Spore syringes are generally for PF cakes and agar round these parts.
Once cleaned on agar you can: drop wedges into wbs Make liquid culture syringe Make liquid inoculant and pour into wbs
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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Re: Shook jars of WBS after innoc. Am I screwed? Re-innoc? [Re: dr_bizzarro]
#24020976 - 01/18/17 06:46 PM (7 years, 12 days ago) |
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It is not recommended. Its not guaranteed to fail, and many people have had many successful grows doing so... but it does have a chance to contaminate. anyone you see posting grows from using spores>grains probably isn't showing you the 3 other grows that contaminated out.
the best method for inoculating grains is to germinate your spores on agar plates (search for Pastywhyte's Easy Agar [aka pasty plates]). once you have growth on agar, you transfer it 2-3 times until its a pure clean mushroom culture, then you drop the colonized agar puck onto grains.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Shook jars of WBS after innoc. Am I screwed? Re-innoc? [Re: ComebackKid]
#24020979 - 01/18/17 06:48 PM (7 years, 12 days ago) |
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i always shake after i use liquid inoculates should i not be? going on years of doing that without contam issues(in jars at least)
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The Mycologist
Explorer

Registered: 05/06/16
Posts: 3,024
Last seen: 30 days, 10 hours
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Re: Shook jars of WBS after innoc. Am I screwed? Re-innoc? [Re: Munchauzen]
#24020983 - 01/18/17 06:49 PM (7 years, 12 days ago) |
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If you have a vendor syringe then its definitely to risky imo. I just feel more comfortable taking two weeks or so to take it to agar.
-------------------- "That you are here—that life exists, and identity; That the powerful play goes on, and you will contribute a verse.” ― Walt Whitman, Leaves of Grass

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Munchauzen


Registered: 06/22/11
Posts: 14,342
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Re: Shook jars of WBS after innoc. Am I screwed? Re-innoc? [Re: mushboy]
#24020987 - 01/18/17 06:50 PM (7 years, 12 days ago) |
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Quote:
mushboy said: i always shake after i use liquid inoculates should i not be? going on years of doing that without contam issues(in jars at least)
he shook spores, not LI. you don't have to shake spores because itll make them germ slower and its harder to spot contams
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Shook jars of WBS after innoc. Am I screwed? Re-innoc? [Re: Munchauzen]
#24021017 - 01/18/17 06:56 PM (7 years, 12 days ago) |
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ive shook spores-grain jars too
since then ive evolved passed such barbaric practices. oh well. learn something new everyday here
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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Re: Shook jars of WBS after innoc. Am I screwed? Re-innoc? [Re: mushboy]
#24021026 - 01/18/17 06:59 PM (7 years, 12 days ago) |
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yeah you gotta remember that spores gotta mate to make mycelium and if you shake then you are spreading the spores out making the distance they have to search out each other that much greater
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Shook jars of WBS after innoc. Am I screwed? Re-innoc? [Re: Munchauzen]
#24021048 - 01/18/17 07:08 PM (7 years, 12 days ago) |
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ill make a note. although i wouldnt do spores-grain ever again.
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dr_bizzarro
Stranger


Registered: 09/10/15
Posts: 28
Last seen: 4 years, 12 days
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Re: Shook jars of WBS after innoc. Am I screwed? Re-innoc? [Re: mushboy]
#24021701 - 01/18/17 11:29 PM (7 years, 12 days ago) |
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AHA!
Thanks for the tips! I'll be getting in to agar asap now. I've been reading and watching vids.
A couple quick questions if anyone has time.
- I didn't see info on agar transfers to result in a "pure clean mushroom culture". That's just agar to agar? It gets stronger each time or something? I can't seem to find a solid post on that procedure. Links? Thanks!
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Rosen_Rot
Learning



Registered: 12/06/14
Posts: 1,225
Loc: Goa
Last seen: 11 months, 22 days
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Re: Shook jars of WBS after innoc. Am I screwed? Re-innoc? [Re: mushboy]
#24021711 - 01/18/17 11:42 PM (7 years, 12 days ago) |
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Quote:
mushboy said: i always shake after i use liquid inoculates should i not be? going on years of doing that without contam issues(in jars at least)


Same here. I always shake after inoculating my jars whether it's syringes or G2G. Never had a problem and had success for years
DO NOT RE-INOCULATE
DO NOT WASTE TIME WAITING
Just work on something else in the meantime and be patient cause spores can take weeks, sometimes even a month before there are any signs of life
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"The internet has one rule; use or be used" - Bjeldiablo ''there is no loneliness, only moments where contentment is fleeting'' SBJs "The Basics" 3iRiS9 "Cirque du freak" B+ BONANZA
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