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Offlinedr_bizzarro
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Shook jars of WBS after innoc. Am I screwed? Re-innoc?
    #24020790 - 01/18/17 05:43 PM (7 years, 12 days ago)

Heyo!

Regarding Franks WBS Tek. 2nd attempt. ("Aborts" last time)

Somehow I got the idea that shaking jars after inoculation is good, as it disperses spores or some such. After doing this, I read that in fact it's not good at all. (Only, Shake right after sterilizing and at 30% colonization, correct?)

I'm 10 days out with no signs of myco at all.

I'm patient, and can wait, but I don't want to wait for nothing.

Should I just chill? I had the weird idea to innoc them AGAIN and then not shake. Does anyone ever do that? Using single layer of tyvek, then polyfill through lid.

They are in open room with indirect dim light, natural FAE (no fans) and at about 70-77 degrees F.


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InvisibleMunchauzen
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Re: Shook jars of WBS after innoc. Am I screwed? Re-innoc? [Re: dr_bizzarro]
    #24020823 - 01/18/17 05:53 PM (7 years, 12 days ago)

Do not re-inoculate. Thats asking for trouble.

I would just get more jars and make a 2nd batch. Multiple grows staggered 1 week apart is a great way to alleviate the grow anxiety


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OfflineDactylium
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Re: Shook jars of WBS after innoc. Am I screwed? Re-innoc? [Re: dr_bizzarro]
    #24020863 - 01/18/17 06:15 PM (7 years, 12 days ago)

Spores can take weeks to germinate. You should be pleased nothing nasty grew considering you used a spore syringe on grain. If the spores do decide to germinate you're likely in the clear as far as contams go.


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Offlinedr_bizzarro
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Re: Shook jars of WBS after innoc. Am I screwed? Re-innoc? [Re: Dactylium]
    #24020934 - 01/18/17 06:34 PM (7 years, 12 days ago)

Quote:

Do not re-inoculate. Thats asking for trouble.

I would just get more jars and make a 2nd batch. Multiple grows staggered 1 week apart is a great way to alleviate the grow anxiety




Ok thanks. Yeah, not a bad idea.

Quote:

You should be pleased nothing nasty grew considering you used a spore syringe on grain. If the spores do decide to germinate you're likely in the clear as far as contams go.




This is the first I've heard about not using spore syringes on WBS. (But I am new, bear with me..) Is there a consensus on that? What's the alternative? I flame and alcohol sterilized of course.


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OfflineDactylium
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Re: Shook jars of WBS after innoc. Am I screwed? Re-innoc? [Re: dr_bizzarro]
    #24020966 - 01/18/17 06:43 PM (7 years, 12 days ago)

Spores are collected in open air environments so bacteria or mold spores could have found their way into the syringe. We germinate our spore solution on a petri dish so we can see the contaminants and isolate the mushroom mycelium from them. There's lots of good teks on here and they aren't very difficult to follow. Agar as easy as 1-2-3 is a great place to start.

You use a still air box right?


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OfflineDactylium
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Re: Shook jars of WBS after innoc. Am I screwed? Re-innoc? [Re: Dactylium]
    #24020967 - 01/18/17 06:44 PM (7 years, 12 days ago)

Everything you need is in Munch's signature if you're interested.


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InvisibleComebackKid
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Re: Shook jars of WBS after innoc. Am I screwed? Re-innoc? [Re: dr_bizzarro]
    #24020975 - 01/18/17 06:46 PM (7 years, 12 days ago)

Spore syringes are generally for PF cakes and agar round these parts.

Once cleaned on agar you can:
drop wedges into wbs
Make liquid culture syringe
Make liquid inoculant and pour into wbs


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InvisibleMunchauzen
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Re: Shook jars of WBS after innoc. Am I screwed? Re-innoc? [Re: dr_bizzarro]
    #24020976 - 01/18/17 06:46 PM (7 years, 12 days ago)

It is not recommended. Its not guaranteed to fail, and many people have had many successful grows doing so... but it does have a chance to contaminate. anyone you see posting grows from using spores>grains probably isn't showing you the 3 other grows that contaminated out.

the best method for inoculating grains is to germinate your spores on agar plates (search for Pastywhyte's Easy Agar [aka pasty plates]). once you have growth on agar, you transfer it 2-3 times until its a pure clean mushroom culture, then you drop the colonized agar puck onto grains.


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InvisiblemushboyMDiscord
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Re: Shook jars of WBS after innoc. Am I screwed? Re-innoc? [Re: ComebackKid]
    #24020979 - 01/18/17 06:48 PM (7 years, 12 days ago)

i always shake after i use liquid inoculates:shrug: should i not be? going on years of doing that without contam issues(in jars at least)


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OfflineThe Mycologist
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Re: Shook jars of WBS after innoc. Am I screwed? Re-innoc? [Re: Munchauzen]
    #24020983 - 01/18/17 06:49 PM (7 years, 12 days ago)

If you have a vendor syringe then its definitely to risky imo. I just feel more comfortable taking two weeks or so to take it to agar.


--------------------
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InvisibleMunchauzen
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Re: Shook jars of WBS after innoc. Am I screwed? Re-innoc? [Re: mushboy]
    #24020987 - 01/18/17 06:50 PM (7 years, 12 days ago)

Quote:

mushboy said:
i always shake after i use liquid inoculates:shrug: should i not be? going on years of doing that without contam issues(in jars at least)



he shook spores, not LI. you don't have to shake spores because itll make them germ slower and its harder to spot contams


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InvisiblemushboyMDiscord
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Re: Shook jars of WBS after innoc. Am I screwed? Re-innoc? [Re: Munchauzen]
    #24021017 - 01/18/17 06:56 PM (7 years, 12 days ago)

ive shook spores-grain jars too:shrug:

since then ive evolved passed such barbaric practices. oh well. learn something new everyday here


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InvisibleMunchauzen
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Re: Shook jars of WBS after innoc. Am I screwed? Re-innoc? [Re: mushboy]
    #24021026 - 01/18/17 06:59 PM (7 years, 12 days ago)

yeah you gotta remember that spores gotta mate to make mycelium and if you shake then you are spreading the spores out making the distance they have to search out each other that much greater


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InvisiblemushboyMDiscord
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Re: Shook jars of WBS after innoc. Am I screwed? Re-innoc? [Re: Munchauzen]
    #24021048 - 01/18/17 07:08 PM (7 years, 12 days ago)

ill make a note. although i wouldnt do spores-grain ever again.


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Offlinedr_bizzarro
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Re: Shook jars of WBS after innoc. Am I screwed? Re-innoc? [Re: mushboy]
    #24021701 - 01/18/17 11:29 PM (7 years, 12 days ago)

AHA!

Thanks for the tips! I'll be getting in to agar asap now. I've been reading and watching vids.

A couple quick questions if anyone has time.

- I didn't see info on agar transfers to result in a "pure clean mushroom culture". That's just agar to agar? It gets stronger each time or something? I can't seem to find a solid post on that procedure. Links? Thanks!

-


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OfflineRosen_Rot
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Re: Shook jars of WBS after innoc. Am I screwed? Re-innoc? [Re: mushboy]
    #24021711 - 01/18/17 11:42 PM (7 years, 12 days ago)

Quote:

mushboy said:
i always shake after i use liquid inoculates:shrug: should i not be? going on years of doing that without contam issues(in jars at least)




:whathesaid:

:laugh2:

Same here. I always shake after inoculating my jars whether it's syringes or G2G. Never had a problem and had success for years

DO NOT RE-INOCULATE

DO NOT WASTE TIME WAITING

Just work on something else in the meantime and be patient cause spores can take weeks, sometimes even a month before there are any signs of life


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