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Offlineclockwork_indigo
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Registered: 12/03/16
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Sterilizing Agar?
    #24020628 - 01/18/17 04:44 PM (7 years, 12 days ago)

Will agar be sterile if you were to steam sterilize it in a normal pot for 90 mins like brf cakes? The same recipe as the one in Pastywhytes easy agar Tek except without the pc.I have a PC just wondering if it would work.

Another question is my PC says it needs a minimum of 3 qts of water in it but when I put that much, even elevated with jar rings the bottom of my agar jars are still sitting in water so can I put less water so my filters won't get wet and my jars won't get so much condensation during the cycle? How long does each qrt of water last?


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OfflineTheDuder
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Re: Sterilizing Agar? [Re: clockwork_indigo]
    #24020657 - 01/18/17 04:53 PM (7 years, 12 days ago)

You only need about an inch of water in the PC for the agar sterilization process.


--------------------

|-------------------[Ps. Azurescens]------------------------------------------[Ps. Semilanceata]--------------------------------------------[Ps. Allenii]------------------------|


|--------------[Ps. Ovoideocystidiata]------------------------------------------[Ps. Stuntzii]--------------------------------------------[Ps. Baeocystis]----------------------|


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InvisiblemushboyMDiscord
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Re: Sterilizing Agar? [Re: TheDuder]
    #24020660 - 01/18/17 04:54 PM (7 years, 12 days ago)

i only use enough water to barely cover the rack inside. so like an inch at most.


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InvisibleMunchauzen
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Re: Sterilizing Agar? [Re: mushboy]
    #24020750 - 01/18/17 05:32 PM (7 years, 12 days ago)

yes you can steam sterilize agar plates but most prefer a PC because it can be done in a fraction of the time


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Offlineclockwork_indigo
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Re: Sterilizing Agar? [Re: Munchauzen]
    #24021000 - 01/18/17 06:52 PM (7 years, 12 days ago)

Would it be the same as brf cakes for the steam sterilizing time 90 mins?


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InvisibleAdden
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Re: Sterilizing Agar? [Re: clockwork_indigo]
    #24021162 - 01/18/17 07:42 PM (7 years, 12 days ago)

I go through a quart of water at 15psi 60 minute mark in my 22qt. There's still some left but I don't like using anything less.


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Offlineclockwork_indigo
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Re: Sterilizing Agar? [Re: Adden]
    #24021253 - 01/18/17 08:10 PM (7 years, 12 days ago)

My pc is a 16 quart. I think the water would last the same amount of time as your 22 quart though


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Offlineclockwork_indigo
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Re: Sterilizing Agar? [Re: clockwork_indigo]
    #24021256 - 01/18/17 08:11 PM (7 years, 12 days ago)

Thanks for the help guys


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InvisibleMunchauzen
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Re: Sterilizing Agar? [Re: Adden]
    #24021263 - 01/18/17 08:12 PM (7 years, 12 days ago)

Quote:

Adden said:
I go through a quart of water at 15psi 60 minute mark in my 22qt. There's still some left but I don't like using anything less.



if you add weight on your jiggler, it will use less water. i can run for 2 hours at 20psi on 1.5 quarts and not run dry.


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Offlineclockwork_indigo
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Re: Sterilizing Agar? [Re: Munchauzen]
    #24021310 - 01/18/17 08:26 PM (7 years, 12 days ago)

Sounds good thanks


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InvisibleAdden
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Re: Sterilizing Agar? [Re: Munchauzen]
    #24021464 - 01/18/17 09:31 PM (7 years, 12 days ago)

Quote:

Munchauzen said:
Quote:

Adden said:
I go through a quart of water at 15psi 60 minute mark in my 22qt. There's still some left but I don't like using anything less.



if you add weight on your jiggler, it will use less water. i can run for 2 hours at 20psi on 1.5 quarts and not run dry.




Nice thank you!


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