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InvisibleLoveshroomz777


Registered: 11/19/16
Posts: 616
Pf tek
    #24015358 - 01/16/17 05:29 PM (7 years, 14 days ago)

So I was unclear on when mixing everything I mix the vermiculite and water first right then I mix in the brown rice flour? Thanks guys


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Offlinewtfcrazymofo
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Re: Pf tek [Re: Loveshroomz777] * 1
    #24015437 - 01/16/17 06:03 PM (7 years, 14 days ago)

Mix all the dry ingredents first, then add water.


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InvisiblemushboyMDiscord
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Re: Pf tek [Re: wtfcrazymofo] * 1
    #24015537 - 01/16/17 06:50 PM (7 years, 14 days ago)

like making a cake...  :thatsallfolks:


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OfflineMycologist217
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Re: Pf tek [Re: mushboy] * 1
    #24015593 - 01/16/17 07:10 PM (7 years, 14 days ago)

Roger Rabbit recommends mixing the water with the vermiculite first and then adding the rice flour to avoid clumping and make sure that the water retaining verm gets a "coating of flour"...i've always done it his way and never been disappointed.


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InvisibleZiran
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Re: Pf tek [Re: Mycologist217]
    #24015596 - 01/16/17 07:11 PM (7 years, 14 days ago)

Quote:

Mycologist217 said:
Roger Rabbit recommends mixing the water with the vermiculite first and then adding the rice flour to avoid clumping and make sure that the water retaining verm gets a "coating of flour"...i've always done it his way and never been disappointed.




This is what I do. Works pretty damn well.


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InvisibleLoveshroomz777


Registered: 11/19/16
Posts: 616
Re: Pf tek [Re: Ziran]
    #24015649 - 01/16/17 07:33 PM (7 years, 14 days ago)

Awesome thanks guys


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OnlineLogicaL ChaosM
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Re: Pf tek [Re: Mycologist217]
    #24015767 - 01/16/17 08:11 PM (7 years, 14 days ago)

Quote:

Mycologist217 said:
Roger Rabbit recommends mixing the water with the vermiculite first and then adding the rice flour to avoid clumping and make sure that the water retaining verm gets a "coating of flour"...i've always done it his way and never been disappointed.




Thats how i do it.

Wet the verm then coating it with BRF afterwords.

gives a less chunky consistency.


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InvisibleAdden
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Re: Pf tek [Re: Loveshroomz777]
    #24015804 - 01/16/17 08:27 PM (7 years, 14 days ago)

I found this in my journal if it helps any. It will help you transition to culture work and it's really hard to mess up jars in a SAB. It'll give you the practice and your Order of Things for when you're in there moving agar.

1. Fill jar with 50/50 brf up to the ring.

2. Smooth out the inner rim and remove nutrition before adding the verm layer. I use the 3 section paper towels and rip them in half, set them on a dry surface, and use one per jar. Don't cross contam, you could be smearing BRF from jar 1 to jar 2.

3. PC at 15 psi for 45 minutes (when the rocker starts, so should your timer).

4. Let them sit overnight to cool. Never remove the rocker until you're ready to load your still air box.

5. Lay a towel down on a table. Spray it with soapy water. Clean out your SAB with 71% ISO, using paper towels not a rag, do it again with soapy water, spray all 5 sides with soapy water. Placing the two open holes against the towel.

6. Unload your PC into your SAB. Your jars are only going to be briefly in the open air just set them in there. Unwrap foil, put in SAB. No need to wipe them down etc you're just adding work and contamination vectors. They are sterile when they come out of the PC.

6a. Wear gloves. Blue nitrile with iso. I usually just iso them once at the beginning of work. (A face mask helps especially if you have facial hair. A shower doesn't hurt. Scrub your arms like you're going into surgery. Rings and especially watches are contamination vectors.

7. Flame your needle outside your SAB, not near the arm holes. Do not wipe with alcohol; fire red hot will kill anything. Eject half a cc to cool the needle. Make sure your needle gets past the dry verm barrier. Inject a drop or two of water and slowly move around the jar (I hold it in my hand and turn it and keep my movements to a minimum).

8. Make sure you work mechanically with slow calculated movements.

9. Flame between jars, not each hole.

10. Use the right jars.

11. Try not to mess with them for two weeks. Trich and bacteria will show in 3 to 4 days, get those out of the house.

12. Make sure you don't leave your SAB to go grab something quick. If you have to leave your area, change your gloves when you come back. Touching a faucet or a doorknob or your phone.. you may as well run your hand in toilet water to clean it off.

13. Don't store contam jars near other projects.

^ just some notes laying around


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OfflineBluetang99
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Registered: 12/27/16
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Re: Pf tek [Re: Mycologist217]
    #24015851 - 01/16/17 08:42 PM (7 years, 14 days ago)


Quote:

Adden said:
I found this in my journal if it helps any. It will help you transition to culture work and it's really hard to mess up jars in a SAB. It'll give you the practice and your Order of Things for when you're in there moving agar.

1. Fill jar with 50/50 brf up to the ring.

2. Smooth out the inner rim and remove nutrition before adding the verm layer. I use the 3 section paper towels and rip them in half, set them on a dry surface, and use one per jar. Don't cross contam, you could be smearing BRF from jar 1 to jar 2.

3. PC at 15 psi for 45 minutes (when the rocker starts, so should your timer).

4. Let them sit overnight to cool. Never remove the rocker until you're ready to load your still air box.

5. Lay a towel down on a table. Spray it with soapy water. Clean out your SAB with 71% ISO, using paper towels not a rag, do it again with soapy water, spray all 5 sides with soapy water. Placing the two open holes against the towel.

6. Unload your PC into your SAB. Your jars are only going to be briefly in the open air just set them in there. Unwrap foil, put in SAB. No need to wipe them down etc you're just adding work and contamination vectors. They are sterile when they come out of the PC.

6a. Wear gloves. Blue nitrile with iso. I usually just iso them once at the beginning of work. (A face mask helps especially if you have facial hair. A shower doesn't hurt. Scrub your arms like you're going into surgery. Rings and especially watches are contamination vectors.

7. Flame your needle outside your SAB, not near the arm holes. Do not wipe with alcohol; fire red hot will kill anything. Eject half a cc to cool the needle. Make sure your needle gets past the dry verm barrier. Inject a drop or two of water and slowly move around the jar (I hold it in my hand and turn it and keep my movements to a minimum).

8. Make sure you work mechanically with slow calculated movements.

9. Flame between jars, not each hole.

10. Use the right jars.

11. Try not to mess with them for two weeks. Trich and bacteria will show in 3 to 4 days, get those out of the house.

12. Make sure you don't leave your SAB to go grab something quick. If you have to leave your area, change your gloves when you come back. Touching a faucet or a doorknob or your phone.. you may as well run your hand in toilet water to clean it off.

13. Don't store contam jars near other projects.

^ just some notes laying around





I followed this route for the most part myself when I did my first grow, but I have some questions.

1. What's the point of hairnet/mask, shower, etc if the jars are in a glove box and you're not hanging over your jars/syringe when you place them in the glove box? Isn't that what the dry verm layer is for, to keep contaminants from falling into the substrate?

2. Also, you say bacteria/contaminants will show in a few days if infected. What would these look like? My jars are starting to show white mycelium after a week but don't see anything else that is suspicious.


Edited by Bluetang99 (01/16/17 08:50 PM)


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InvisibleAdden
I'm a teapot
Registered: 06/04/03
Posts: 39,201
Loc: Flag
Re: Pf tek [Re: Bluetang99]
    #24015923 - 01/16/17 09:07 PM (7 years, 14 days ago)

Still air box*

And it's not very necessary but the important part is getting in your groove for your SAB work. It really eliminates a lot of things that could go wrong. Or people who wipe jars with 70% iso right out of the PC, it makes no sense.

Really just practice I guess for your upcoming agar work. Might as well eliminate contamination vectors and get used to being in there and what you should/shouldn't be doing. Also, if they fail you know it's the syringes or you or something else.


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OfflineBluetang99
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Registered: 12/27/16
Posts: 179
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Re: Pf tek [Re: Adden]
    #24015936 - 01/16/17 09:11 PM (7 years, 14 days ago)

Makes sense. I used one I made out of a cardboard box for now and hoping it went well. I see little spots of mycelium growing so I'm pretty excited.


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InvisibleAdden
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Posts: 39,201
Loc: Flag
Re: Pf tek [Re: Bluetang99]
    #24015947 - 01/16/17 09:16 PM (7 years, 14 days ago)

Nice. The best thing to have is patience. The furthest I've gotten in this hobby is doing cakes and culture work. The other half of my time is picking seasons. It's nice to have psilocybes on hand. Culture work is fascinating. I figure if I can do agar work then anyone can. It took me 9 transfers but I got spore germination on a map I picked up while out picking one day. Every step in this hobby is an achievement no matter which way you go. I'll tell you though it's awesome to see these guys flushing pounds a day, haha.


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OfflineMycologist217
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Re: Pf tek [Re: Bluetang99] * 2
    #24015948 - 01/16/17 09:17 PM (7 years, 14 days ago)

consider making this thread into a grow log that you can continue to ask questions on and update on the progress of your grow. Don't be afraid to upload pictures to the shroomery and include them with your questions.


--------------------
My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M
AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE

Check out my Retail Gourmet Mushroom Farm!

Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.


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InvisibleLoveshroomz777


Registered: 11/19/16
Posts: 616
Re: Pf tek [Re: Mycologist217]
    #24015998 - 01/16/17 09:41 PM (7 years, 14 days ago)

If I don't have a alcohol flame for my needle can I just use a lighter or a 99.9% Lysol alcohol swab


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OnlineLogicaL ChaosM
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Re: Pf tek [Re: Loveshroomz777]
    #24016005 - 01/16/17 09:42 PM (7 years, 14 days ago)

I use 99% iso alcohol no flame.

Most people think its taboo but i do it anywayz :shrug:


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InvisibleLoveshroomz777


Registered: 11/19/16
Posts: 616
Re: Pf tek [Re: LogicaL Chaos]
    #24016019 - 01/16/17 09:48 PM (7 years, 14 days ago)

Will a Lysol swab work it says it kills 99.9% of bacteria


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OfflineMycologist217
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Re: Pf tek [Re: LogicaL Chaos]
    #24016027 - 01/16/17 09:52 PM (7 years, 14 days ago)

alchol flame lamps are super easy to build....

just a cotton ball pulled through a hole stabbed into a garlic jar lid...filled with denatured alcohol...but 91% iso will work too.


--------------------
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AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE

Check out my Retail Gourmet Mushroom Farm!

Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.


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OnlineLogicaL ChaosM
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Re: Pf tek [Re: Loveshroomz777]
    #24016028 - 01/16/17 09:52 PM (7 years, 14 days ago)

Yah should be fine.

It does carry some risk as flaming kills it all. But it should fine if u wipe with Lysol between each jar.


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InvisibleLoveshroomz777


Registered: 11/19/16
Posts: 616
Re: Pf tek [Re: LogicaL Chaos]
    #24016050 - 01/16/17 10:01 PM (7 years, 14 days ago)

So will the 99% alcohol be better than the Lysol swab


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OnlineLogicaL ChaosM
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Re: Pf tek [Re: Loveshroomz777]
    #24016073 - 01/16/17 10:13 PM (7 years, 14 days ago)

Not sure honesty. Probably about the same.


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