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OfflineSlit Sniff
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Mono's contaminating 2-3 weeks after spawning?
    #24015283 - 01/16/17 04:52 PM (7 years, 14 days ago)

My mono's seemed to be doing great, but all 10 mono's got trich at some point between 2-3 weeks after spawning. 1:3 ratio of 100% healthy/fluffy mycelium of WBS to 50/50 coir/verm which was PC'd for 1 hour. They were 100% in 6 days, but I waited 7 days to fruit/insert poly-fil.

I had 4 mono's doing especially well with threaded myc crawling up the walls + thick, hearty fruits at pinning stage and others halfway grown when a small area showed signs of trich. I salted the areas and still got a decent harvest, but more tossing than dunking after these flushes. Only 2 tubs didn't yield much or anything at all.


If not the bulk being bad, then it has to be a spawn issue. Temps in my grow room at consistent at 73-76 with a small fan pointed away from the mono's. I heard that the air gets stale in mono's even with the 4 poly-holes at fruiting level and a poly-hole on each end at the top to allow more oxygen in.

Is it possible that PC'ing bulk is the cause for such late contamination? Like I said 2-3 weeks after spawning/1-2 weeks after fruiting.


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OfflinePinPornProducer
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Re: Mono's contaminating 2-3 weeks after spawning? [Re: Slit Sniff]
    #24015305 - 01/16/17 05:02 PM (7 years, 14 days ago)

Pc for a minimum of 90 minutes @ 15psi. I do 2hrs. What are you inoculating with?


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InvisiblemushboyMDiscord
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Re: Mono's contaminating 2-3 weeks after spawning? [Re: PinPornProducer]
    #24015343 - 01/16/17 05:23 PM (7 years, 14 days ago)

dirty spawn will trich out way before any 'stale air' issues happen.

clean up that spawn. otherwise you will continue with those results. every once in awhile you will have good tubs but the trich will always find you.


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Offlinewtfcrazymofo
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Re: Mono's contaminating 2-3 weeks after spawning? [Re: Slit Sniff]
    #24015506 - 01/16/17 06:33 PM (7 years, 14 days ago)

Your coir turned green.  You can't sterilize bulk unless you have a hepa filtered automated room.


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InvisibleZiran
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Re: Mono's contaminating 2-3 weeks after spawning? [Re: Slit Sniff]
    #24015598 - 01/16/17 07:13 PM (7 years, 14 days ago)

pasteurize it not sterilize.


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OfflineSlit Sniff
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Re: Mono's contaminating 2-3 weeks after spawning? [Re: mushboy]
    #24017139 - 01/17/17 11:32 AM (7 years, 13 days ago)

Quote:

mushboy said:
dirty spawn will trich out way before any 'stale air' issues happen.




So the fact that the mono went bad so late into the grow, that would mean it's from the coir/verm then? I was just stating that I've heard about the stale air/moisture. My main question is if the spawn or bulk is the issue, since I PC'd the bulk instead of sterilized.

Also, if dirty spawn contaminates "way before" then it would've contaminated before the 2-3 week mark after spawning the mono.


--------------------
I'm still not good at this and it's been almost 7 years.
Shit's hard, like golf, but more... "woke."



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OfflineSlit Sniff
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Re: Mono's contaminating 2-3 weeks after spawning? [Re: PinPornProducer]
    #24017151 - 01/17/17 11:36 AM (7 years, 13 days ago)

Quote:

PinPornProducer said:
Pc for a minimum of 90 minutes @ 15psi. I do 2hrs. What are you inoculating with?




I did actually PC a few bulk subs at 90 min @ 15psi by accident since I'm so used to PC'ing WBS for 90 min. Still all mono's went bad very late in the grow. I'm used to having bad spawn, where my grow gets trich at 10-14 days after spawning the mono.

Wouldn't sterilizing the coir/verm make it even more susceptible to contams? I'm having a hard time figuring out if there is in fact benefits to pasteurizing coir and vermiculite or if it has nothing to offer if pasteurized.


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I'm still not good at this and it's been almost 7 years.
Shit's hard, like golf, but more... "woke."



Edited by Slit Sniff (01/17/17 11:37 AM)


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InvisibleMr Piggy
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Re: Mono's contaminating 2-3 weeks after spawning? [Re: Slit Sniff]
    #24017156 - 01/17/17 11:38 AM (7 years, 13 days ago)

Quote:

Slit Sniff said:
1:3 ratio of 100% healthy/fluffy mycelium of WBS to 50/50 coir/verm which was PC'd for 1 hour.




Are you saying you sterilized your 50/50 mix instead of bucket pasteurizing it?


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InvisiblemushboyMDiscord
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Re: Mono's contaminating 2-3 weeks after spawning? [Re: Slit Sniff]
    #24017159 - 01/17/17 11:40 AM (7 years, 13 days ago)

Quote:

Slit Sniff said:
Quote:

mushboy said:
dirty spawn will trich out way before any 'stale air' issues happen.




So the fact that the mono went bad so late into the grow, that would mean it's from the coir/verm then? I was just stating that I've heard about the stale air/moisture. My main question is if the spawn or bulk is the issue, since I PC'd the bulk instead of sterilized.

Also, if dirty spawn contaminates "way before" then it would've contaminated before the 2-3 week mark after spawning the mono.




no its not from the cvg. cv doesnt really go bad. thats the point. its in the wbs spawn.


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InvisiblebodhisattaMDiscordReddit
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Re: Mono's contaminating 2-3 weeks after spawning? [Re: mushboy]
    #24017165 - 01/17/17 11:43 AM (7 years, 13 days ago)

You see it 10-14 after spawning. It was there all along.
That's not late into the grow


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OfflineSlit Sniff
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Re: Mono's contaminating 2-3 weeks after spawning? [Re: bodhisatta]
    #24017989 - 01/17/17 05:33 PM (7 years, 13 days ago)

Quote:

bodhisatta said:
You see it 10-14 after spawning. It was there all along.
That's not late into the grow




I mean that I've had bad spawn before, which resulted in 10-14 days after spawning.

In my current situation, they didn't go bad until about 3 weeks after spawning. This is why I suspect that the problem lies in my bulk, not spawn. Trich didn't show up until the fruits were halfway grown or even when I was about to harvest.


--------------------
I'm still not good at this and it's been almost 7 years.
Shit's hard, like golf, but more... "woke."



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OfflineSlit Sniff
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Re: Mono's contaminating 2-3 weeks after spawning? [Re: Mr Piggy]
    #24017998 - 01/17/17 05:36 PM (7 years, 13 days ago)

Quote:

Mr Piggy said:
Quote:

Slit Sniff said:
1:3 ratio of 100% healthy/fluffy mycelium of WBS to 50/50 coir/verm which was PC'd for 1 hour.




Are you saying you sterilized your 50/50 mix instead of bucket pasteurizing it?




Correct. I PC'd my bulk instead of pasteurizing. Would PC'ing bulk, make it more susceptible to contams since it has no "defense" against mold spores? Or does it even matter since coir/verm don't really have any nutrients anyway?


--------------------
I'm still not good at this and it's been almost 7 years.
Shit's hard, like golf, but more... "woke."



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OfflinePinPornProducer
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Re: Mono's contaminating 2-3 weeks after spawning? [Re: Slit Sniff]
    #24018040 - 01/17/17 06:01 PM (7 years, 13 days ago)

Imo mold right away would mean moldy spawn, mold that doesn't show up till first flush means bacterial spawn that allowed mold to take over later on.

Coir can be pcd, I did it for over a year with no issues. Its just an overkill, simply mix it in a bucket, boil enough water to bring it to field capacity, pour it in the bucket and put the lid on it. Wait 2-3 hrs, mix real good and tub it


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