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Offlinebeggsnham
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Registered: 10/10/16
Posts: 49
Last seen: 6 years, 3 months
stinky agar!! no!
    #24015138 - 01/16/17 03:53 PM (7 years, 14 days ago)

Hello, I have here a dish of agar with what was suppose to be an ape hybrid cubensis (albino penis envy) and I have a STRONG belief of a contamination.
Inoculated just yesterday with a spore syringe I dropped several drops onto agar surface

However, I have a few suspicions that bring about some questions

First, I have a thought that maybe my syring itself was contaminated but only used one time with great success with no contamination the first use.
Question 1: how likely is it to contaminate a spore syringe if was clean to begin with?

Another thought, the agar may have been stored improperly and allowed condensation to set on gelatin surface and could have caused the contaminated agar.

Question 2: how is proper storing suppose to happen with agar and also, how likely would that condensation build up cause a contamination?
And finally the smell, it's rancid rotten smell entire room stinks bad ... I was rather blown away

any help would be greatly appreciated.

Novice grower
few small grows successful in past



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InvisibleMycolorado
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Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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Re: stinky agar!! no! [Re: beggsnham]
    #24015196 - 01/16/17 04:15 PM (7 years, 14 days ago)

No way that impressive crop of bacteria colonies grew since yesterday.

Syringes are often dirty.


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InvisiblebodhisattaMDiscordReddit
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Registered: 04/30/13
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Re: stinky agar!! no! [Re: Mycolorado]
    #24015606 - 01/16/17 07:17 PM (7 years, 14 days ago)

Ape syringes are inherently more contaminated because of the spore harvest technique. Spores should be streak plated rather than just dropped onto a dish if there's a problem with contamination


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OfflineThedillestpickle
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Registered: 02/02/16
Posts: 1,170
Loc: Canada Flag
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Re: stinky agar!! no! [Re: bodhisatta]
    #24015643 - 01/16/17 07:31 PM (7 years, 14 days ago)

That petri dish is stinking up a whole room?  lol what?  you must have a very sensitive nose

how did you use the ape syringe previously?  if PF tek it may have already been contaminated but you got lucky and the myc outgrew the bacteria. 

It wouldn't really be that hard to contaminate a syringe, if you touched the tip to anything, bacteria could possibly have consumed spores as food and populated the whole syringe.


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