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Colonel
Mycotographer



Registered: 11/04/16
Posts: 317
Loc: PNW, Washington
Last seen: 2 months, 2 days
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Colonel's Roatan/Honduras LC 1
#24014287 - 01/16/17 10:40 AM (7 years, 14 days ago) |
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I started with 2 x 400ml Mason Jars and drilled 2 holes in each lid. Then placed 1 x 1/4" Compression Insert into one hole in each lid putting it into place with a Heat Resistant Silicone. Then on the other hole i placed a fair amount of silicone over the entire hole creating a Self Healing Inoculation Port. Once this was completed it looked liked this..

At this point I crimped the end of the Compression Insert with a wrench and stuffed a small ball of Polyfil into the open end of the insert covering the hole with two layers of Micropore Tape.

At this point the jars themselves were ready I then had to mix up my culture. I filled the jars up with 300ml/400ml of hot water and placed 15g of Honey in each stirring until the honey was completely dissolved.

I then Placed the jars into my PC for 20 minutes and let them cool overnight. Once cooled i placed one in the fridge for later use and the other i decided to Inoculate with Roatan/Honduras Cubensis. I flame sterilized my needle as well as sterilizing the port with alcohol and inserted 1cc of spores via Syringe.

Now we wait for further growth, i will update the thread when possible! 
It is now 1/19/2017 and there is distinct mycelial growth in the LC!

It is now 1/23/2017 and I'm thinking this LC is about complete.

I plan to inoculate to rye tomorrow and will update with photos when possible!
Edited by Colonel (01/23/17 04:04 PM)
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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Re: Colonel's Roatan/Honduras LC [Re: Colonel]
#24014295 - 01/16/17 10:44 AM (7 years, 14 days ago) |
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You had me until you inoculated with spores.
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Colonel
Mycotographer



Registered: 11/04/16
Posts: 317
Loc: PNW, Washington
Last seen: 2 months, 2 days
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Re: Colonel's Roatan/Honduras LC [Re: Munchauzen]
#24014301 - 01/16/17 10:48 AM (7 years, 14 days ago) |
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Saving the other jar for my pasty plates which are germinating now

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P. Ovoideocystidiata P. Cyanescens P. Northwest Colonel's Roatan/Honduras LC Thai Lipa Yai Monotub Grow
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Colonel's Roatan/Honduras LC [Re: Munchauzen]
#24014603 - 01/16/17 12:41 PM (7 years, 14 days ago) |
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Quote:
Munchauzen said: You had me until you inoculated with spores.

if you have the plates just wait to test the inoculate on agar first. then knock up the lc with a clean wedge.
spore syringe to lc is like not bagging it after you get home with that skanky bitch from the club.
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Colonel
Mycotographer



Registered: 11/04/16
Posts: 317
Loc: PNW, Washington
Last seen: 2 months, 2 days
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Re: Colonel's Roatan/Honduras LC [Re: mushboy]
#24014619 - 01/16/17 12:47 PM (7 years, 14 days ago) |
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I know its risky but i just decided i would go both ways and see how either one turns out. Growth on the plates look great though! Will be updating the threat with both results from agar to LC and Spore to LC.
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P. Ovoideocystidiata P. Cyanescens P. Northwest Colonel's Roatan/Honduras LC Thai Lipa Yai Monotub Grow
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Colonel
Mycotographer



Registered: 11/04/16
Posts: 317
Loc: PNW, Washington
Last seen: 2 months, 2 days
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Re: Colonel's Roatan/Honduras LC [Re: Colonel]
#24019153 - 01/18/17 05:21 AM (7 years, 12 days ago) |
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Update: Just noticed a ball of rhizomorphic growth inside the LC  I have high hopes, and ill update with photos later.
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P. Ovoideocystidiata P. Cyanescens P. Northwest Colonel's Roatan/Honduras LC Thai Lipa Yai Monotub Grow
Edited by Colonel (01/19/17 11:27 AM)
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Colonel
Mycotographer



Registered: 11/04/16
Posts: 317
Loc: PNW, Washington
Last seen: 2 months, 2 days
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Re: Colonel's Roatan/Honduras LC [Re: Colonel]
#24022666 - 01/19/17 11:08 AM (7 years, 11 days ago) |
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Update: It is now 1/19/2017 and there is distinct mycelial growth in the LC!
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P. Ovoideocystidiata P. Cyanescens P. Northwest Colonel's Roatan/Honduras LC Thai Lipa Yai Monotub Grow
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TheDuder
Mushroom Hunter



Registered: 11/07/16
Posts: 2,544
Loc: PNW
Last seen: 1 year, 18 days
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Re: Colonel's Roatan/Honduras LC [Re: Colonel]
#24022715 - 01/19/17 11:29 AM (7 years, 11 days ago) |
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Nice job dude, looking good!
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|-------------------[Ps. Azurescens]------------------------------------------[Ps. Semilanceata]--------------------------------------------[Ps. Allenii]------------------------|
|--------------[Ps. Ovoideocystidiata]------------------------------------------[Ps. Stuntzii]--------------------------------------------[Ps. Baeocystis]----------------------|
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Colonel
Mycotographer



Registered: 11/04/16
Posts: 317
Loc: PNW, Washington
Last seen: 2 months, 2 days
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Re: Colonel's Roatan/Honduras LC [Re: TheDuder]
#24033618 - 01/23/17 04:05 PM (7 years, 7 days ago) |
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Update: I'm thinking this LC is about complete.

I plan to inoculate to rye tomorrow and will update with photos when possible!
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P. Ovoideocystidiata P. Cyanescens P. Northwest Colonel's Roatan/Honduras LC Thai Lipa Yai Monotub Grow
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