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Tiamo
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Another run-in with contaminants on agar, where am I going wrong?! *DELETED*
#24013777 - 01/16/17 04:46 AM (7 years, 14 days ago) |
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Post deleted by TiamoReason for deletion: privacy
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Edited by Tiamo (01/16/17 04:49 AM)
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synthetik
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Tiamo]
#24013838 - 01/16/17 06:33 AM (7 years, 14 days ago) |
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im a fucking newb so take this with a grain of salt, but one thing that stuck out when i read this was your use of 90% etoh as a sanitizer, I did some reading and to make it short stick with 70% iso
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AlCapone2k
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: synthetik] 1
#24013844 - 01/16/17 06:40 AM (7 years, 14 days ago) |
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1. Get yourself some Borosilikat-Bottles, for laboratory with the blue lid.
2. Never open your Petri Sleve outside the SAB, just open it inside and get the petries out of it
3. Use a soapy-water soaked towel as the bottom of the SAB so that everythings sticks into it.
4. Try to put tin foil (not the first cm of the roll, throw it away and take then take the foil) on the soakd towel so that nothing that you use has to be put on the towel which is full of contamns.
5. Put everything you will use on the tin foil and THEN put the soaked SAB on it.
Maybe that could help
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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spore-ty



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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: synthetik]
#24013847 - 01/16/17 06:43 AM (7 years, 14 days ago) |
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Shower first (which im doing test runs to see how neccessaru this as opposed to just washing hands/ forearms
But shower first Wipe your contents down with 70% Put them in sab put 70% iso towel on top of them and spray your sab let sit for 30 seconds
And pouring petri or inoculating you want to do them in stacks
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mushboy
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: spore-ty]
#24013866 - 01/16/17 07:01 AM (7 years, 14 days ago) |
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if you are putting on gloves why shower? or wash hands for that matter.
something else is up. your practices sound good but something has to be happening. not trying to state the obvious or anything.... opening the sleeve outside sab could be it?
im surprised you havent said fuck it and tried pastyplates. or have you?
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AlCapone2k
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: mushboy]
#24013876 - 01/16/17 07:08 AM (7 years, 14 days ago) |
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Tiamo lives in Europe, actually I don't know if there are pastyplates around here, I haven't seen them yet
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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mushboy
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: AlCapone2k]
#24013878 - 01/16/17 07:10 AM (7 years, 14 days ago) |
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i ment no pour agar.
i dont pour my dishes because i am a dumbass and ill spill inside my sab. like a child with milk.guaranteed.
dont they sell sterilized and unsterilized petris?
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AgarStudent
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: AlCapone2k]
#24013880 - 01/16/17 07:11 AM (7 years, 14 days ago) |
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Build a laminar flow hood and that should give you much better results.
-------------------- Why reinvent the wheel when someone here has already done the science?
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mushboy
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: AgarStudent]
#24013881 - 01/16/17 07:13 AM (7 years, 14 days ago) |
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Quote:
AgarStudent said: Build a laminar flow hood and that should give you much better results.
nah. thats throwing away a lot of money for a solution that doesnt fit the problem.
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AlCapone2k
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: AgarStudent]
#24013883 - 01/16/17 07:13 AM (7 years, 14 days ago) |
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A LFH is not necessary if it fails on pouring agar. Agar work can be done in a SAB without any problems, thousands of people do it, so he doesn't have to spend money on a LFH.
If he can afford it, why not, sure, but if Pouring petris is not possible yet, he sould try to get better in sterile work, not spend money on a LFH.
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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Tiamo
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: mushboy]
#24013884 - 01/16/17 07:15 AM (7 years, 14 days ago) |
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I'm actually PCing some no-pour right now.
I open my sleeve outside the SAB because it's 33 plates, not 20. I can't put it all in the SAB, it's too big. This may sounds noobish, but it shouldn't be too much of an issue. The lids remain on the dishes, so nothing can get in right? They're only outside the SAB for seconds and get wiped down when they're in.
I have to note that not all of my failed attempts were due to bad pours. It's almost always something different, but my petris almost always seem to contaminate anyway.
Either way, I'm taking note of everyone's advice. I'll definitely integrate these tips. 
@AlCapone, I can find cheap PP5 containers in Action, then drill a hole in the side under the lid and put micropore tape on it.
--------------------
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mushboy
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Tiamo]
#24013888 - 01/16/17 07:17 AM (7 years, 14 days ago) |
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Quote:
Tiamo said: @AlCapone, I can find cheap PP5 containers in Action, then drill a hole in the side under the lid and put micropore tape on it.
perfect. i like the square ones.

btw, do you notice the irony in having stros how to agar thread linked in your sig?
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AlCapone2k
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Tiamo]
#24013890 - 01/16/17 07:18 AM (7 years, 14 days ago) |
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Buddy if you have problems opening the sleeve inside the SAB, just lay it on your work desk inside the SAB, cut the sleeve in the middle and get the Petris out from the middle.
The petries inside the sleeve don't have to be pulled out from the top
I'll do it always like this.
I really think this is your problem, that you open the sleeve outside.
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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Tiamo
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: mushboy]
#24013897 - 01/16/17 07:24 AM (7 years, 14 days ago) |
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Unfortunately, I see the irony. I've read just about every thread in this forum yet my results are mediocre to non existent. One day I'll get good like the rest of you. 
@AlCapone Thanks for the tip, I'll try it. Because all my shit contaminates I've only been pouring 5 petris at a time because I don't want to put all my eggs in one basket, so to say. I still have one unopened sleeve left. But I don't want to pour them all. How you you propose storing the half that I don't pour?
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AlCapone2k
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Tiamo]
#24013908 - 01/16/17 07:30 AM (7 years, 14 days ago) |
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Just let the unused ones inside the sleeve, roll the sleeve a bit and close it with a food clip
Just try to pour all the petris though, when following the tipps in this thread, you will be sucessful
And try to get petris in a 20-package, then you won't have the problem to open the sleeve inside the SAB
Make a picture of your SAB please
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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NDStepp84
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Tiamo]
#24013910 - 01/16/17 07:32 AM (7 years, 14 days ago) |
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What size is your SAB? 105 qt or better with big armholes around 5- 6" or more FTW. The tall ones are great, I could g2g half gallons

I kept getting satellite contams and it was driving me nuts, couldn't figure it out. Ended up being my SAB was too small with too small of arm holes.
--------------------
"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do. -Robert A. Heinlein
Links and teks ND's grow log and discussion Plant thread
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mushboy
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Tiamo]
#24013913 - 01/16/17 07:34 AM (7 years, 14 days ago) |
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Quote:
Tiamo said: One day I'll get good like the rest of you. 
over 10 years id say its taken me a solid 2years of added up fails. must be. ive fucked up everything so bad. all i can do now is grow them. ive fucked everything possible up to the point where im out of things to fuck up.
now im growing pans and some stone producers. lets see how hard i fail at those first time
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Tiamo
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: NDStepp84]
#24013921 - 01/16/17 07:41 AM (7 years, 14 days ago) |
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This is my SAB. It is 130 L. I use it on a standing desk, and it is very comfortable and easy to move around in and see. I use it like this, with a plastic lid. But I'll use a towel next time.
--------------------
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AlCapone2k
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Tiamo]
#24013934 - 01/16/17 07:49 AM (7 years, 14 days ago) |
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yeah, use a towel and you will get rid of all the problems
Just search for Spitball Jedis SAB Tek and do it like he describes it
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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Mycolorado
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: mushboy]
#24013945 - 01/16/17 07:54 AM (7 years, 14 days ago) |
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One thing that stands out to me is the number of time you're touching the plates and moving them around and wiping them. I would suggest you take 5 at a time out in a stack and set them in the box. Spray if you have to. While maintaining the stack, pour into the bottom plate by sandwiching the top plates and the bottom plate lid together so you can crack the bottom plate open 45 degrees and pour in...if that makes sense. Repeat this til you reach the top. This should drastically improve your plate contam rate...not sure why your bottle's contaminating.
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NDStepp84
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: mushboy]
#24013966 - 01/16/17 08:11 AM (7 years, 14 days ago) |
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Quote:
Tiamo said: This is my SAB. It is 130 L. I use it on a standing desk, and it is very comfortable and easy to move around in and see. I use it like this, with a plastic lid. But I'll use a towel next time.

That's a nice SAB
Quote:
mushboy said:
Quote:
Tiamo said: One day I'll get good like the rest of you. 
over 10 years id say its taken me a solid 2years of added up fails. must be. ive fucked up everything so bad. all i can do now is grow them. ive fucked everything possible up to the point where im out of things to fuck up.
now im growing pans and some stone producers. lets see how hard i fail at those first time
I bet you learned a hell of a lot from those fails at least.
--------------------
"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do. -Robert A. Heinlein
Links and teks ND's grow log and discussion Plant thread
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mushboy
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: NDStepp84]
#24013971 - 01/16/17 08:15 AM (7 years, 14 days ago) |
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Quote:
NDStepp84 said: I bet you learned a hell of a lot from those fails at least.
function over design is one of them.

if my sab works. tiamo should be good. 2kAL might be right about opening the sleeves outside the box.
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spore-ty



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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: mushboy]
#24013972 - 01/16/17 08:15 AM (7 years, 14 days ago) |
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Quote:
mushboy said: if you are putting on gloves why shower? or wash hands for that
>
Im assuming you still wear gloves? Ive done my past couple g2gs bare handed anybody else tried this? Gonna be trying with agar soon
Edited by spore-ty (01/16/17 08:16 AM)
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mushboy
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: spore-ty]
#24013979 - 01/16/17 08:18 AM (7 years, 14 days ago) |
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what? no, of course i use gloves. but i dont wash my hands if i am using them. its pointless.
i would not recommend doing bare handed agar work.
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spore-ty



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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: mushboy]
#24014009 - 01/16/17 08:27 AM (7 years, 14 days ago) |
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Quote:
mushboy said: what? no, of course i use gloves. but i dont wash my hands if i am using them. its pointless.
i would not recommend doing bare handed agar work.
Ya I figured but I like to see what nasties I got 
With a FH would you say a shower is required before each use
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Tookitooki
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Tiamo]
#24014071 - 01/16/17 09:03 AM (7 years, 14 days ago) |
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I used to get all crazy about being sterile in my SAB. Each time I keep dialing back cause I like to be as lazy as possible.
1. Use 70% ISO as stated above. Reason being, (IIRC) anything higher, evaporates to fast before it can penetrate and kill the bad spores. The water content of 70% helps the ISO get thru the spores allowing the ISO to do it's job.
2. A towel is good and bad. Setting all your tools and supplies on a wet soapy towel is a way to transfer contams. I did that a few times and sprayed my SAB down with soapy water. I had a water droplets fall from the top and land right in a agar dish I was pouring. The bottoms of everything I grabbed had a soapy film on them. I lost 5 dishes the first time I did a sleeve, the second time I lost 2.
My solution was to grab paper towels, spray them with the soapy water instead of my SAB, and then wipe my SAB down. Making sure to not leave droplets on the top or walls. I ditched the towel, and just wipe the lid in the same manner.
Once everything is wiped and loaded in the box. I drape wet paper towels over the arm holes and let everything sit for at least 5 mins. I put on gloves, I wear a clean long sleeve shirt, tuck my sleeves under the gloves and spray my arms lightly with soapy water. Rub them down so no water droplets are there, I ISO my gloves and go to work.
Move slow and diligent, when removing arms to flame any tools.
I wipe the outside of the sleeves of Plates with ISO once I load them in. I also wipe my flask of agar down as well.
I don't lose many plates anymore, and when I do, it's cause I'm stoned off ass and get sloppy when pouring the plates.
Persistence pays off, keep at it, changing variables and refining your technique along the way, untill you find success. Good luck and keep us posted
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mushboy
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Tookitooki]
#24014090 - 01/16/17 09:12 AM (7 years, 14 days ago) |
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thats alot. honestly i just..
1.get box. spray walls with cold water with tiny bit of dish soap. like a drop for a whole windex sprayer.
2.using a papertowel soaked in ISO i wipe the lid area of everything going inside. wipe tools and whatever else. put tools ontop of jars. not on the floor. or use spare jar rings as areas to rest things. KEEP OFF THE FLOOR
3.let box become still in 10min ish? ive never timed. just when im ready.
4. work quick!! im not even being careful sometimes. quicker the better. by not being careful i mean like, ill pop open a pastyplate lid and just let it fall on the floor of the SAB and just leave it there. or ill just drop the syringe on the floor of the box and move to the next item.
thats it..
i keep 3-4 soaked paper towels of iso outside the box to wipe my hands. no jar or plate is left open or IS open for more than a second. after i handle ANYTHING i wipe my gloved hands with iso. my gloved hands are wet in iso at all times. be careful glass becomes slick.
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cronicr



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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: mushboy]
#24024732 - 01/20/17 04:32 AM (7 years, 10 days ago) |
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Gloves are fine but not needed..your hands should never be going over your work anyway.
I use a wet towel no need for the soap we use the soap for the rest of the sab.
Work with confidence.
Post pics of these contams..satellite contams often come from blade handles because you only flame the tip.
When you spray your sab your knocking down contams so no need to wait too long.
--------------------
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Tiamo
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: cronicr]
#24024737 - 01/20/17 04:37 AM (7 years, 10 days ago) |
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Quote:
cronicr said: Post pics of these contams..satellite contams often come from blade handles because you only flame the tip.
They're pretty impossible to photograph. And no blade was involved in these contaminants. Just pouring. Even the bottle and wrapped unused plates were toast with bacteria.
I've been thinking. Last run I did in my SAB I used pasty plates and a towel. I sprayed with hot water and soap and used a PC rack as a work platform. But, at the end of the session I felt the walls. They had completely dried. Is that a bad thing? How do I keep them wet all the time?
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cronicr



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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Tiamo]
#24024808 - 01/20/17 06:26 AM (7 years, 10 days ago) |
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Why did u use hot water? you dont want heat currents..the soap helps evaporation and if it dries give it another squirt
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wtfcrazymofo
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Tiamo]
#24024815 - 01/20/17 06:41 AM (7 years, 10 days ago) |
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After you prep your sab, if it is in a still air room I might take ten minutes for all the contams to settle out of the air and onto the floor in the still air box. Try to give your sab the extra time to chill before you load your gear and get to work.
Take off sleeve inside the box.
Holding the stack of nine and the tenth's lid do your pour, then 8 and ninth's lid pour the 9th until you pour the last one on top of the stack of ten.
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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Terpfreak
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: wtfcrazymofo]
#24024821 - 01/20/17 06:50 AM (7 years, 10 days ago) |
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Sounds like all the little issues I had to work out with practice. You're getting there, keep at it!
--------------------
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Tiamo
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: cronicr]
#24024870 - 01/20/17 07:32 AM (7 years, 10 days ago) |
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I read hot water was preferred, but the water cools so fast it barely matters. I really appreciate all the advice guys, I'm definitely keeping at it and I will work out all my issues eventually.
--------------------
If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
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FunGuyZ
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: spore-ty]
#24025090 - 01/20/17 09:03 AM (7 years, 10 days ago) |
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Quote:
spore-ty said:
Quote:
mushboy said: if you are putting on gloves why shower? or wash hands for that
>
Im assuming you still wear gloves? Ive done my past couple g2gs bare handed anybody else tried this? Gonna be trying with agar soon
I've done all my agar work barehanded. Doesn't seem to be a problem.
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Tiamo
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Tiamo]
#24027312 - 01/21/17 03:09 AM (7 years, 9 days ago) |
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I've got to thinking... The glass agar bottles I've been using to pour used to contain milk. I cleaned them out as well as I could but the bacteria that keeps showing up smells really sour not unlike Lactobacillus and it kinda looks like it too. Have I been breading the remnants of that milk the entire time or am I just being silly? I'm gonna order some borosilicate glass.
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If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
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Mycolorado
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Tiamo]
#24027483 - 01/21/17 07:41 AM (7 years, 9 days ago) |
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If you're PCing it then the answer is no. How are you capping the bottle...lid, cotton, foil? Have you ran a control and just let the bottle sit fir a few days after PCing? Have you run any control plates? These things would help you narrow down the vector. If nothing grows in the controls, then it's your technique and you migh look back at my post on plating in a stack and handling the plates less.
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Tiamo
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Mycolorado]
#24027488 - 01/21/17 07:45 AM (7 years, 9 days ago) |
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I haven't made a control bottle, but I have made control plates. I get very mixed results. First run no plates contaminated. Second run some did and third run a few did. 
I am capping the bottle with a screw top and put foil over it. PC for 30 mins.
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Mycolorado
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Tiamo]
#24027494 - 01/21/17 07:48 AM (7 years, 9 days ago) |
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Are you wiping the bottle down like you said you were wiping the plates? That is one (major) vector that could be introducing contams.
Are you pcing the cap?...sorry asking all that comes to mind...
Edited by Mycolorado (01/21/17 07:52 AM)
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Tiamo
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Mycolorado]
#24027506 - 01/21/17 07:59 AM (7 years, 9 days ago) |
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No I wasn't wiping them down, and I've only wiped plates once and understand I shouldn't do it anymore. Of course I am PCing the cap. I'll still get some borosilicate glass and try again in a few weeks. The bottles are just nicer in general than some 100mL store bottles with milk.
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Mycolorado
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Tiamo]
#24027519 - 01/21/17 08:08 AM (7 years, 9 days ago) |
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Good luck
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germanauslander
Forever Learner

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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Mycolorado]
#24029530 - 01/22/17 01:28 AM (7 years, 8 days ago) |
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@Tiamo out of curiosity what were you using to seal your Petris?
Saran Wrap or Parafilm?
This is one thing that I am not sure of what to do, since a lot of people recommend Saran Wrap, but then others don't saying that it doesn't allow for Gas Exchange.
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AlCapone2k
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: germanauslander] 1
#24029623 - 01/22/17 03:35 AM (7 years, 8 days ago) |
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You could use both, don't sorry about that. If you can afford Parafilm, get it
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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Tiamo
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: AlCapone2k]
#24029638 - 01/22/17 04:04 AM (7 years, 8 days ago) |
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I've been researching petri dishes and I realised my petris weren't gamma sterilised. The shop I bought them from charged me €5 for a 33 pack and had them marked as "sterile". But I googled and found out they are "aseptic" and weren't sterilised with gamma rays. I honestly think this is where all my problems stem from. I have no idea if these petris are sterile enough for mycology work. The gamma sterilised petris are about twice as expensive so there must be something to them. 
I also found "machine sterile" petris and was wondering if those are good enough? Or do you need "gamma sterile"?
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Edited by Tiamo (01/22/17 04:11 AM)
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blindingleaf
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Tiamo]
#24029645 - 01/22/17 04:12 AM (7 years, 8 days ago) |
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damn dude, that sucks if that is the situation.
I would go talk to the shop and tell them what happened so maybe u can get replacements, or at the very least, you school them on what "Sterile" means so it doesn't happen to another customer.
every plate I ever got came in sealed sleeves of 20-25. if they don't look like that, I won't order them.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Tiamo
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: blindingleaf]
#24029646 - 01/22/17 04:14 AM (7 years, 8 days ago) |
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The description from the supplier (translated) reads:
"Petri dishes are produced with side-ways injection moulding (temperature of 250 degrees) under laminar flow and immediately packaged"
So that would be a no-go? I've no clue about this shit.
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blindingleaf
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Tiamo]
#24029651 - 01/22/17 04:25 AM (7 years, 8 days ago) |
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haha me either dude. I just order a case on e bay, but I know u are in EU so might be different.
I'd shoot them an e mail and tell them what u wanna do with it, and ask if that product is right or if there is another one they would suggest. tell them what happened with the other ones so they know what you are trying to avoid.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Tiamo
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: blindingleaf]
#24029662 - 01/22/17 04:49 AM (7 years, 8 days ago) |
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Can you link me the case you bought? Trying to figure out this sterilisation stuff. So far it seems there are 4 categories: Gamma sterilisation, ethylene oxide sterilisation/chemical sterilisation, machine sterilisation/aseptic production, non-sterile. Probably in that order of reliability.
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blindingleaf
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Tiamo]
#24029664 - 01/22/17 04:55 AM (7 years, 8 days ago) |
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I dont have a standard brand or set that I get. whatever is cheapest on "buy now" on e bay for a case is what I go for-and shipping from within the US.
here is an example
edit: obviously, u'd want shipping from within EU not US. I just don't order from overseas because the long distance usually means more broken plates.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
Edited by blindingleaf (01/22/17 05:03 AM)
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Mycolorado
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: blindingleaf]
#24029811 - 01/22/17 07:54 AM (7 years, 8 days ago) |
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Pay a bit more and get Falcon. They're the best. I've ordered cheap ones before and they are shit.
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Intelligentxfruit
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Posts: 1,545
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Mycolorado]
#24029842 - 01/22/17 08:17 AM (7 years, 8 days ago) |
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Reading over this I couldn't see a problem with your technique. I open my sleeves outside the sab all the time with no problem Id put a bet down on it being the petris not being sterile
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Mycolorado
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Intelligentxfruit]
#24029844 - 01/22/17 08:19 AM (7 years, 8 days ago) |
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We need to figure out why his agar bottle is contaminating...
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lsbexperience
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: NDStepp84]
#24029845 - 01/22/17 08:19 AM (7 years, 8 days ago) |
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i get my spore prints from ebay, cheaper, and never contaminated!
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Intelligentxfruit
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Mycolorado]
#24029858 - 01/22/17 08:25 AM (7 years, 8 days ago) |
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Quote:
Mycolorado said: We need to figure out why his agar bottle is contaminating...
Yeah thats the odd missing piece I cant connect Maybe new petris along with the towel. I never used a towel either though so idk man
I didnt see any mention of the design of your agar pour bottle. What filter material are you using? Could it be compromised?
Edited by Intelligentxfruit (01/22/17 08:28 AM)
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Tiamo
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Intelligentxfruit]
#24029941 - 01/22/17 09:06 AM (7 years, 8 days ago) |
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Not using a filter and it's not necessary for agar. Enlightenment pours from borosilicate bottles, fills them almost all the way and covers them with foil. https://www.shroomery.org/forums/showflat.php/Number/23890579 I've only had my bottle contaminate once. Maybe just a fluke. I've also had some clean plates. Maybe just flukes. Who knows. I'm looking for some gamma sterilised plates now.
Mean while, my no pour plates have not contaminated. Showing no growth yet but all my petris would contaminate by this point. I think I'm good on them.
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bodhisatta 
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Tiamo]
#24029974 - 01/22/17 09:16 AM (7 years, 8 days ago) |
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I don't use a dumb lid on my agar either. Just the regular cap that goes on a media bottle designed for things like sterilizing and pouring agar from
https://www.shroomery.org/forums/showflat.php/Number/21922023
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Intelligentxfruit
Earth Hippy


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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: bodhisatta]
#24030003 - 01/22/17 09:29 AM (7 years, 8 days ago) |
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I do but I reuse any jar I can and some lids sketch me out. I have no clue why your pour bottle got contams though
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spore-ty



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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: cronicr]
#24030109 - 01/22/17 10:17 AM (7 years, 8 days ago) |
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Quote:
cronicr said: Gloves are fine but not needed..your hands should never be going over your work anyway.
I use a wet towel no need for the soap we use the soap for the rest of the sab.
Work with confidence.
Post pics of these contams..satellite contams often come from blade handles because you only flame the tip.
When you spray your sab your knocking down contams so no need to wait too long.
Do you PC your whole scalpel everytime?
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b1blazin
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: spore-ty]
#24030360 - 01/22/17 12:10 PM (7 years, 8 days ago) |
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flame sterilization of scalpel is necessary between each incision. Ive always been good with just a wipe down using 91 Isopropyl Alc.
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cronicr



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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: spore-ty]
#24030827 - 01/22/17 03:07 PM (7 years, 8 days ago) |
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Quote:
spore-ty said:
Quote:
cronicr said: Gloves are fine but not needed..your hands should never be going over your work anyway.
I use a wet towel no need for the soap we use the soap for the rest of the sab.
Work with confidence.
Post pics of these contams..satellite contams often come from blade handles because you only flame the tip.
When you spray your sab your knocking down contams so no need to wait too long.
Do you PC your whole scalpel everytime?
I PCs a jar full of scalpels
--------------------
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Intelligentxfruit
Earth Hippy


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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: cronicr]
#24030884 - 01/22/17 03:25 PM (7 years, 8 days ago) |
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I know you use some dirty ass scalpels over there
Jk lol
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AlCapone2k
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Intelligentxfruit]
#24032621 - 01/23/17 07:11 AM (7 years, 7 days ago) |
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Machine sterile is absolutely enough, I buy machine sterile agar plates and never had a contamn that I didn't cause.
Gamma-sterile of course is a bit better, but not necessary.
I buy my plates for 4 Euro / 20 pcs.
I wonder why the agar bottle is contaminating, I think it's just the bad filter/method. Have you bought some Borosilikat-Bottles like I told you in my first answer?
Regards
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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Tiamo
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: AlCapone2k]
#24032667 - 01/23/17 07:51 AM (7 years, 7 days ago) |
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Good to know that machinesterile is good enough for mycology work. I have purchased some borosilicate bottles but I think I'll be doing no-pour for a while. I actually really like this method and the fact I can reuse the plates, even if visibility is very poor.
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If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
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spore-ty



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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: cronicr]
#24032703 - 01/23/17 08:22 AM (7 years, 7 days ago) |
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Quote:
cronicr said:
Quote:
spore-ty said:
Quote:
cronicr said: Gloves are fine but not needed..your hands should never be going over your work anyway.
I use a wet towel no need for the soap we use the soap for the rest of the sab.
Work with confidence.
Post pics of these contams..satellite contams often come from blade handles because you only flame the tip.
When you spray your sab your knocking down contams so no need to wait too long.
Do you PC your whole scalpel everytime?
I PCs a jar full of scalpels
Lmao should've figured who I was talking to but all ive got is Paul stamets favorite scalpel should I get a bunch a keep em sterilized at all times or can I keep my one scalpel and just wipe down with 71% and sterilize every other time?
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AlCapone2k
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Tiamo]
#24032708 - 01/23/17 08:25 AM (7 years, 7 days ago) |
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Don't overcomplicate it
I sterilize my scalpel handle once every two weeks when sterilizing agar anyway
Wiping it with ISP like all the other stuff is enough. The blade itself is flam sterilized anyway and the only thing touching agar.
Try to learn not to go with the handle of the scalpel over open media unless it's necessary, then you should be fine with only wiping the handle + flame-sterilzing the blade
Regards
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
Edited by AlCapone2k (01/23/17 08:26 AM)
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bodhisatta 
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: AlCapone2k]
#24032720 - 01/23/17 08:39 AM (7 years, 7 days ago) |
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 I've never sterilized a scalpel. Wipe with alcohol the blade gets flamed
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