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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Intelligentxfruit]
#24032621 - 01/23/17 07:11 AM (7 years, 7 days ago) |
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Machine sterile is absolutely enough, I buy machine sterile agar plates and never had a contamn that I didn't cause.
Gamma-sterile of course is a bit better, but not necessary.
I buy my plates for 4 Euro / 20 pcs.
I wonder why the agar bottle is contaminating, I think it's just the bad filter/method. Have you bought some Borosilikat-Bottles like I told you in my first answer?
Regards
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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Tiamo
Trust in LITFA



Registered: 04/07/16
Posts: 1,935
Loc: Amsterdam
Last seen: 5 hours, 4 minutes
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: AlCapone2k]
#24032667 - 01/23/17 07:51 AM (7 years, 7 days ago) |
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Good to know that machinesterile is good enough for mycology work. I have purchased some borosilicate bottles but I think I'll be doing no-pour for a while. I actually really like this method and the fact I can reuse the plates, even if visibility is very poor.
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If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
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spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: cronicr]
#24032703 - 01/23/17 08:22 AM (7 years, 7 days ago) |
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Quote:
cronicr said:
Quote:
spore-ty said:
Quote:
cronicr said: Gloves are fine but not needed..your hands should never be going over your work anyway.
I use a wet towel no need for the soap we use the soap for the rest of the sab.
Work with confidence.
Post pics of these contams..satellite contams often come from blade handles because you only flame the tip.
When you spray your sab your knocking down contams so no need to wait too long.
Do you PC your whole scalpel everytime?
I PCs a jar full of scalpels
Lmao should've figured who I was talking to but all ive got is Paul stamets favorite scalpel should I get a bunch a keep em sterilized at all times or can I keep my one scalpel and just wipe down with 71% and sterilize every other time?
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Tiamo]
#24032708 - 01/23/17 08:25 AM (7 years, 7 days ago) |
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Don't overcomplicate it
I sterilize my scalpel handle once every two weeks when sterilizing agar anyway
Wiping it with ISP like all the other stuff is enough. The blade itself is flam sterilized anyway and the only thing touching agar.
Try to learn not to go with the handle of the scalpel over open media unless it's necessary, then you should be fine with only wiping the handle + flame-sterilzing the blade
Regards
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
Edited by AlCapone2k (01/23/17 08:26 AM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: AlCapone2k]
#24032720 - 01/23/17 08:39 AM (7 years, 7 days ago) |
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 I've never sterilized a scalpel. Wipe with alcohol the blade gets flamed
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