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Offlinegermanauslander
Forever Learner

Registered: 11/06/16
Posts: 100
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Mycolorado]
    #24029530 - 01/22/17 01:28 AM (7 years, 9 days ago)

@Tiamo out of curiosity what were you using to seal your Petris?

Saran Wrap or Parafilm?

This is one thing that I am not sure of what to do, since a lot of people recommend Saran Wrap, but then others don't saying that it doesn't allow for Gas Exchange.


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OfflineAlCapone2k
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: germanauslander] * 1
    #24029623 - 01/22/17 03:35 AM (7 years, 8 days ago)

You could use both, don't sorry about that. If you can afford Parafilm, get it :smile:


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


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OfflineTiamo
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: AlCapone2k]
    #24029638 - 01/22/17 04:04 AM (7 years, 8 days ago)

I've been researching petri dishes and I realised my petris weren't gamma sterilised. The shop I bought them from charged me €5 for a 33 pack and had them marked as "sterile". But I googled and found out they are "aseptic" and weren't sterilised with gamma rays. I honestly think this is where all my problems stem from. I have no idea if these petris are sterile enough for mycology work. The gamma sterilised petris are about twice as expensive so there must be something to them. :facepalm:

I also found "machine sterile" petris and was wondering if those are good enough? Or do you need "gamma sterile"?


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If you have used a Miraculix Psilocybin QTest, could you please share your results?

Shipping free Ps. natalensis spore prints to any address in The Netherlands, just :pm:

:mushroom2: Mush love :mushroom2:


Edited by Tiamo (01/22/17 04:11 AM)


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Invisibleblindingleaf
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Tiamo]
    #24029645 - 01/22/17 04:12 AM (7 years, 8 days ago)

damn dude, that sucks if that is the situation.

I would go talk to the shop and tell them what happened so maybe u can get replacements, or at the very least, you school them on what "Sterile" means so it doesn't happen to another customer.

every plate I ever got came in sealed sleeves of 20-25.  if they don't look like that, I won't order them.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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OfflineTiamo
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: blindingleaf]
    #24029646 - 01/22/17 04:14 AM (7 years, 8 days ago)

The description from the supplier (translated) reads:

"Petri dishes are produced with side-ways injection moulding (temperature of 250 degrees) under laminar flow and immediately packaged"

So that would be a no-go? I've no clue about this shit.


--------------------


If you have used a Miraculix Psilocybin QTest, could you please share your results?

Shipping free Ps. natalensis spore prints to any address in The Netherlands, just :pm:

:mushroom2: Mush love :mushroom2:


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Invisibleblindingleaf
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Tiamo]
    #24029651 - 01/22/17 04:25 AM (7 years, 8 days ago)

haha me either dude. I just order a case on e bay, but I know u are in EU so might be different.

I'd shoot them an e mail and tell them what u wanna do with it, and ask if that product is right or if there is another one they would suggest.  tell them what happened with the other ones so they know what you are trying to avoid.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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OfflineTiamo
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: blindingleaf]
    #24029662 - 01/22/17 04:49 AM (7 years, 8 days ago)

Can you link me the case you bought? Trying to figure out this sterilisation stuff. So far it seems there are 4 categories: Gamma sterilisation, ethylene oxide sterilisation/chemical sterilisation, machine sterilisation/aseptic production, non-sterile. Probably in that order of reliability.


--------------------


If you have used a Miraculix Psilocybin QTest, could you please share your results?

Shipping free Ps. natalensis spore prints to any address in The Netherlands, just :pm:

:mushroom2: Mush love :mushroom2:


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Invisibleblindingleaf
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Tiamo]
    #24029664 - 01/22/17 04:55 AM (7 years, 8 days ago)

I dont have a standard brand or set that I get.  whatever is cheapest on "buy now" on e bay for a case is what I go for-and shipping from within the US.

here is an example

edit: obviously, u'd want shipping from within EU not US.  I just don't order from overseas because the long distance usually means more broken plates.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


Edited by blindingleaf (01/22/17 05:03 AM)


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InvisibleMycolorado
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: blindingleaf]
    #24029811 - 01/22/17 07:54 AM (7 years, 8 days ago)

Pay a bit more and get Falcon.  They're the best. I've ordered cheap ones before and they are shit.


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InvisibleIntelligentxfruit
Earth Hippy


Registered: 01/06/13
Posts: 1,545
Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Mycolorado]
    #24029842 - 01/22/17 08:17 AM (7 years, 8 days ago)

Reading over this I couldn't see a problem with your technique.
I open my sleeves outside the sab all the time with no problem:shrug:
Id put a bet down on it being the petris not being sterile


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InvisibleMycolorado
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Intelligentxfruit]
    #24029844 - 01/22/17 08:19 AM (7 years, 8 days ago)

We need to figure out why his agar bottle is contaminating...


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Offlinelsbexperience
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: NDStepp84]
    #24029845 - 01/22/17 08:19 AM (7 years, 8 days ago)

i get my spore prints from ebay, cheaper, and never contaminated!


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InvisibleIntelligentxfruit
Earth Hippy


Registered: 01/06/13
Posts: 1,545
Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Mycolorado]
    #24029858 - 01/22/17 08:25 AM (7 years, 8 days ago)

Quote:

Mycolorado said:
We need to figure out why his agar bottle is contaminating...



Yeah thats the odd missing piece I cant connect
Maybe new petris along with the towel.
I never used a towel either though so idk man:shrug:


I didnt see any mention of the design of your agar pour bottle. What filter material are you using? Could it be compromised?


Edited by Intelligentxfruit (01/22/17 08:28 AM)


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OfflineTiamo
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Intelligentxfruit]
    #24029941 - 01/22/17 09:06 AM (7 years, 8 days ago)

Not using a filter and it's not necessary for agar. Enlightenment pours from borosilicate bottles, fills them almost all the way and covers them with foil. https://www.shroomery.org/forums/showflat.php/Number/23890579 I've only had my bottle contaminate once. Maybe just a fluke. I've also had some clean plates. Maybe just flukes. Who knows. I'm looking for some gamma sterilised plates now.

Mean while, my no pour plates have not contaminated. Showing no growth yet but all my petris would contaminate by this point. I think I'm good on them.


--------------------


If you have used a Miraculix Psilocybin QTest, could you please share your results?

Shipping free Ps. natalensis spore prints to any address in The Netherlands, just :pm:

:mushroom2: Mush love :mushroom2:


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InvisiblebodhisattaMDiscordReddit
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Tiamo]
    #24029974 - 01/22/17 09:16 AM (7 years, 8 days ago)

I don't use a dumb lid on my agar either. Just the regular cap that goes on a media bottle designed for things like sterilizing and pouring agar from

https://www.shroomery.org/forums/showflat.php/Number/21922023


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InvisibleIntelligentxfruit
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Registered: 01/06/13
Posts: 1,545
Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: bodhisatta]
    #24030003 - 01/22/17 09:29 AM (7 years, 8 days ago)

I do but I reuse any jar I can and some lids sketch me out.
I have no clue why your pour bottle got contams though


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Offlinespore-ty
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Registered: 01/21/16
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: cronicr]
    #24030109 - 01/22/17 10:17 AM (7 years, 8 days ago)

Quote:

cronicr said:
Gloves are fine but not needed..your hands should never be going over your work anyway.

I use a wet towel no need for the soap we use the soap for the rest of the sab.

Work with confidence.

Post pics of these contams..satellite contams often come from blade handles because you only flame the tip.

When you spray your sab your knocking down contams so no need to wait too long.





Do you PC your whole scalpel everytime?


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Invisibleb1blazin
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Registered: 06/16/12
Posts: 399
Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: spore-ty]
    #24030360 - 01/22/17 12:10 PM (7 years, 8 days ago)

flame sterilization of scalpel is necessary between each incision. Ive always been good with just a wipe down using 91 Isopropyl Alc.


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OfflinecronicrFacebook
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: spore-ty]
    #24030827 - 01/22/17 03:07 PM (7 years, 8 days ago)

Quote:

spore-ty said:
Quote:

cronicr said:
Gloves are fine but not needed..your hands should never be going over your work anyway.

I use a wet towel no need for the soap we use the soap for the rest of the sab.

Work with confidence.

Post pics of these contams..satellite contams often come from blade handles because you only flame the tip.

When you spray your sab your knocking down contams so no need to wait too long.





Do you PC your whole scalpel everytime?



I PCs a jar full of scalpels


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleIntelligentxfruit
Earth Hippy


Registered: 01/06/13
Posts: 1,545
Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: cronicr]
    #24030884 - 01/22/17 03:25 PM (7 years, 8 days ago)

:bsflag:I know you use some dirty ass scalpels over there:rofldrunk:


Jk lol


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