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NDStepp84
Stellar nuclear waste

Registered: 04/23/15
Posts: 4,956
Last seen: 1 month, 4 days
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: mushboy]
#24013966 - 01/16/17 08:11 AM (7 years, 14 days ago) |
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Quote:
Tiamo said: This is my SAB. It is 130 L. I use it on a standing desk, and it is very comfortable and easy to move around in and see. I use it like this, with a plastic lid. But I'll use a towel next time.

That's a nice SAB
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mushboy said:
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Tiamo said: One day I'll get good like the rest of you. 
over 10 years id say its taken me a solid 2years of added up fails. must be. ive fucked up everything so bad. all i can do now is grow them. ive fucked everything possible up to the point where im out of things to fuck up.
now im growing pans and some stone producers. lets see how hard i fail at those first time
I bet you learned a hell of a lot from those fails at least.
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"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do. -Robert A. Heinlein
Links and teks ND's grow log and discussion Plant thread
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: NDStepp84]
#24013971 - 01/16/17 08:15 AM (7 years, 14 days ago) |
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Quote:
NDStepp84 said: I bet you learned a hell of a lot from those fails at least.
function over design is one of them.

if my sab works. tiamo should be good. 2kAL might be right about opening the sleeves outside the box.
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spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: mushboy]
#24013972 - 01/16/17 08:15 AM (7 years, 14 days ago) |
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Quote:
mushboy said: if you are putting on gloves why shower? or wash hands for that
>
Im assuming you still wear gloves? Ive done my past couple g2gs bare handed anybody else tried this? Gonna be trying with agar soon
Edited by spore-ty (01/16/17 08:16 AM)
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: spore-ty]
#24013979 - 01/16/17 08:18 AM (7 years, 14 days ago) |
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what? no, of course i use gloves. but i dont wash my hands if i am using them. its pointless.
i would not recommend doing bare handed agar work.
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spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: mushboy]
#24014009 - 01/16/17 08:27 AM (7 years, 14 days ago) |
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mushboy said: what? no, of course i use gloves. but i dont wash my hands if i am using them. its pointless.
i would not recommend doing bare handed agar work.
Ya I figured but I like to see what nasties I got 
With a FH would you say a shower is required before each use
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Tookitooki
Mycological Fabricator



Registered: 07/28/16
Posts: 1,157
Loc: Nowhere
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Tiamo]
#24014071 - 01/16/17 09:03 AM (7 years, 14 days ago) |
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I used to get all crazy about being sterile in my SAB. Each time I keep dialing back cause I like to be as lazy as possible.
1. Use 70% ISO as stated above. Reason being, (IIRC) anything higher, evaporates to fast before it can penetrate and kill the bad spores. The water content of 70% helps the ISO get thru the spores allowing the ISO to do it's job.
2. A towel is good and bad. Setting all your tools and supplies on a wet soapy towel is a way to transfer contams. I did that a few times and sprayed my SAB down with soapy water. I had a water droplets fall from the top and land right in a agar dish I was pouring. The bottoms of everything I grabbed had a soapy film on them. I lost 5 dishes the first time I did a sleeve, the second time I lost 2.
My solution was to grab paper towels, spray them with the soapy water instead of my SAB, and then wipe my SAB down. Making sure to not leave droplets on the top or walls. I ditched the towel, and just wipe the lid in the same manner.
Once everything is wiped and loaded in the box. I drape wet paper towels over the arm holes and let everything sit for at least 5 mins. I put on gloves, I wear a clean long sleeve shirt, tuck my sleeves under the gloves and spray my arms lightly with soapy water. Rub them down so no water droplets are there, I ISO my gloves and go to work.
Move slow and diligent, when removing arms to flame any tools.
I wipe the outside of the sleeves of Plates with ISO once I load them in. I also wipe my flask of agar down as well.
I don't lose many plates anymore, and when I do, it's cause I'm stoned off ass and get sloppy when pouring the plates.
Persistence pays off, keep at it, changing variables and refining your technique along the way, untill you find success. Good luck and keep us posted
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Tookitooki]
#24014090 - 01/16/17 09:12 AM (7 years, 14 days ago) |
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thats alot. honestly i just..
1.get box. spray walls with cold water with tiny bit of dish soap. like a drop for a whole windex sprayer.
2.using a papertowel soaked in ISO i wipe the lid area of everything going inside. wipe tools and whatever else. put tools ontop of jars. not on the floor. or use spare jar rings as areas to rest things. KEEP OFF THE FLOOR
3.let box become still in 10min ish? ive never timed. just when im ready.
4. work quick!! im not even being careful sometimes. quicker the better. by not being careful i mean like, ill pop open a pastyplate lid and just let it fall on the floor of the SAB and just leave it there. or ill just drop the syringe on the floor of the box and move to the next item.
thats it..
i keep 3-4 soaked paper towels of iso outside the box to wipe my hands. no jar or plate is left open or IS open for more than a second. after i handle ANYTHING i wipe my gloved hands with iso. my gloved hands are wet in iso at all times. be careful glass becomes slick.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: mushboy]
#24024732 - 01/20/17 04:32 AM (7 years, 10 days ago) |
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Gloves are fine but not needed..your hands should never be going over your work anyway.
I use a wet towel no need for the soap we use the soap for the rest of the sab.
Work with confidence.
Post pics of these contams..satellite contams often come from blade handles because you only flame the tip.
When you spray your sab your knocking down contams so no need to wait too long.
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Tiamo
Trust in LITFA




Registered: 04/07/16
Posts: 1,935
Loc: Amsterdam
Last seen: 10 hours, 22 minutes
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: cronicr]
#24024737 - 01/20/17 04:37 AM (7 years, 10 days ago) |
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Quote:
cronicr said: Post pics of these contams..satellite contams often come from blade handles because you only flame the tip.
They're pretty impossible to photograph. And no blade was involved in these contaminants. Just pouring. Even the bottle and wrapped unused plates were toast with bacteria.
I've been thinking. Last run I did in my SAB I used pasty plates and a towel. I sprayed with hot water and soap and used a PC rack as a work platform. But, at the end of the session I felt the walls. They had completely dried. Is that a bad thing? How do I keep them wet all the time?
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If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Tiamo]
#24024808 - 01/20/17 06:26 AM (7 years, 10 days ago) |
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Why did u use hot water? you dont want heat currents..the soap helps evaporation and if it dries give it another squirt
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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wtfcrazymofo
foil hater



Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
Last seen: 14 hours, 47 minutes
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Tiamo]
#24024815 - 01/20/17 06:41 AM (7 years, 10 days ago) |
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After you prep your sab, if it is in a still air room I might take ten minutes for all the contams to settle out of the air and onto the floor in the still air box. Try to give your sab the extra time to chill before you load your gear and get to work.
Take off sleeve inside the box.
Holding the stack of nine and the tenth's lid do your pour, then 8 and ninth's lid pour the 9th until you pour the last one on top of the stack of ten.
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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Terpfreak
❀Terpenes❀



Registered: 07/08/16
Posts: 1,065
Loc: Land Of Ooo
Last seen: 5 years, 10 months
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: wtfcrazymofo]
#24024821 - 01/20/17 06:50 AM (7 years, 10 days ago) |
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Sounds like all the little issues I had to work out with practice. You're getting there, keep at it!
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Tiamo
Trust in LITFA




Registered: 04/07/16
Posts: 1,935
Loc: Amsterdam
Last seen: 10 hours, 22 minutes
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: cronicr]
#24024870 - 01/20/17 07:32 AM (7 years, 10 days ago) |
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I read hot water was preferred, but the water cools so fast it barely matters. I really appreciate all the advice guys, I'm definitely keeping at it and I will work out all my issues eventually.
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If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
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FunGuyZ
Psilly Rabbit


Registered: 12/13/16
Posts: 177
Loc: down the rabbit hole
Last seen: 4 years, 9 months
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: spore-ty]
#24025090 - 01/20/17 09:03 AM (7 years, 10 days ago) |
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Quote:
spore-ty said:
Quote:
mushboy said: if you are putting on gloves why shower? or wash hands for that
>
Im assuming you still wear gloves? Ive done my past couple g2gs bare handed anybody else tried this? Gonna be trying with agar soon
I've done all my agar work barehanded. Doesn't seem to be a problem.
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Tiamo
Trust in LITFA




Registered: 04/07/16
Posts: 1,935
Loc: Amsterdam
Last seen: 10 hours, 22 minutes
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Tiamo]
#24027312 - 01/21/17 03:09 AM (7 years, 9 days ago) |
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I've got to thinking... The glass agar bottles I've been using to pour used to contain milk. I cleaned them out as well as I could but the bacteria that keeps showing up smells really sour not unlike Lactobacillus and it kinda looks like it too. Have I been breading the remnants of that milk the entire time or am I just being silly? I'm gonna order some borosilicate glass.
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If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Tiamo]
#24027483 - 01/21/17 07:41 AM (7 years, 9 days ago) |
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If you're PCing it then the answer is no. How are you capping the bottle...lid, cotton, foil? Have you ran a control and just let the bottle sit fir a few days after PCing? Have you run any control plates? These things would help you narrow down the vector. If nothing grows in the controls, then it's your technique and you migh look back at my post on plating in a stack and handling the plates less.
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Tiamo
Trust in LITFA




Registered: 04/07/16
Posts: 1,935
Loc: Amsterdam
Last seen: 10 hours, 22 minutes
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Mycolorado]
#24027488 - 01/21/17 07:45 AM (7 years, 9 days ago) |
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I haven't made a control bottle, but I have made control plates. I get very mixed results. First run no plates contaminated. Second run some did and third run a few did. 
I am capping the bottle with a screw top and put foil over it. PC for 30 mins.
--------------------
If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Tiamo]
#24027494 - 01/21/17 07:48 AM (7 years, 9 days ago) |
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Are you wiping the bottle down like you said you were wiping the plates? That is one (major) vector that could be introducing contams.
Are you pcing the cap?...sorry asking all that comes to mind...
Edited by Mycolorado (01/21/17 07:52 AM)
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Tiamo
Trust in LITFA




Registered: 04/07/16
Posts: 1,935
Loc: Amsterdam
Last seen: 10 hours, 22 minutes
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Mycolorado]
#24027506 - 01/21/17 07:59 AM (7 years, 9 days ago) |
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No I wasn't wiping them down, and I've only wiped plates once and understand I shouldn't do it anymore. Of course I am PCing the cap. I'll still get some borosilicate glass and try again in a few weeks. The bottles are just nicer in general than some 100mL store bottles with milk.
--------------------
If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Tiamo]
#24027519 - 01/21/17 08:08 AM (7 years, 9 days ago) |
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Good luck
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