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Tiamo
Trust in LITFA




Registered: 04/07/16
Posts: 1,935
Loc: Amsterdam
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Another run-in with contaminants on agar, where am I going wrong?! *DELETED*
#24013777 - 01/16/17 04:46 AM (7 years, 14 days ago) |
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Post deleted by TiamoReason for deletion: privacy
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If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
Edited by Tiamo (01/16/17 04:49 AM)
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synthetik
biological entity

Registered: 12/15/16
Posts: 194
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Tiamo]
#24013838 - 01/16/17 06:33 AM (7 years, 14 days ago) |
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im a fucking newb so take this with a grain of salt, but one thing that stuck out when i read this was your use of 90% etoh as a sanitizer, I did some reading and to make it short stick with 70% iso
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: synthetik] 1
#24013844 - 01/16/17 06:40 AM (7 years, 14 days ago) |
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1. Get yourself some Borosilikat-Bottles, for laboratory with the blue lid.
2. Never open your Petri Sleve outside the SAB, just open it inside and get the petries out of it
3. Use a soapy-water soaked towel as the bottom of the SAB so that everythings sticks into it.
4. Try to put tin foil (not the first cm of the roll, throw it away and take then take the foil) on the soakd towel so that nothing that you use has to be put on the towel which is full of contamns.
5. Put everything you will use on the tin foil and THEN put the soaked SAB on it.
Maybe that could help
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: synthetik]
#24013847 - 01/16/17 06:43 AM (7 years, 14 days ago) |
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Shower first (which im doing test runs to see how neccessaru this as opposed to just washing hands/ forearms
But shower first Wipe your contents down with 70% Put them in sab put 70% iso towel on top of them and spray your sab let sit for 30 seconds
And pouring petri or inoculating you want to do them in stacks
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: spore-ty]
#24013866 - 01/16/17 07:01 AM (7 years, 14 days ago) |
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if you are putting on gloves why shower? or wash hands for that matter.
something else is up. your practices sound good but something has to be happening. not trying to state the obvious or anything.... opening the sleeve outside sab could be it?
im surprised you havent said fuck it and tried pastyplates. or have you?
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AlCapone2k
Beginner


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Loc: Germany
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: mushboy]
#24013876 - 01/16/17 07:08 AM (7 years, 14 days ago) |
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Tiamo lives in Europe, actually I don't know if there are pastyplates around here, I haven't seen them yet
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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mushboy
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Registered: 04/24/05
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: AlCapone2k]
#24013878 - 01/16/17 07:10 AM (7 years, 14 days ago) |
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i ment no pour agar.
i dont pour my dishes because i am a dumbass and ill spill inside my sab. like a child with milk.guaranteed.
dont they sell sterilized and unsterilized petris?
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AgarStudent
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Registered: 01/25/15
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: AlCapone2k]
#24013880 - 01/16/17 07:11 AM (7 years, 14 days ago) |
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Build a laminar flow hood and that should give you much better results.
-------------------- Why reinvent the wheel when someone here has already done the science?
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mushboy
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Registered: 04/24/05
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: AgarStudent]
#24013881 - 01/16/17 07:13 AM (7 years, 14 days ago) |
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Quote:
AgarStudent said: Build a laminar flow hood and that should give you much better results.
nah. thats throwing away a lot of money for a solution that doesnt fit the problem.
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AlCapone2k
Beginner


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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: AgarStudent]
#24013883 - 01/16/17 07:13 AM (7 years, 14 days ago) |
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A LFH is not necessary if it fails on pouring agar. Agar work can be done in a SAB without any problems, thousands of people do it, so he doesn't have to spend money on a LFH.
If he can afford it, why not, sure, but if Pouring petris is not possible yet, he sould try to get better in sterile work, not spend money on a LFH.
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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Tiamo
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Registered: 04/07/16
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: mushboy]
#24013884 - 01/16/17 07:15 AM (7 years, 14 days ago) |
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I'm actually PCing some no-pour right now.
I open my sleeve outside the SAB because it's 33 plates, not 20. I can't put it all in the SAB, it's too big. This may sounds noobish, but it shouldn't be too much of an issue. The lids remain on the dishes, so nothing can get in right? They're only outside the SAB for seconds and get wiped down when they're in.
I have to note that not all of my failed attempts were due to bad pours. It's almost always something different, but my petris almost always seem to contaminate anyway.
Either way, I'm taking note of everyone's advice. I'll definitely integrate these tips. 
@AlCapone, I can find cheap PP5 containers in Action, then drill a hole in the side under the lid and put micropore tape on it.
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If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Tiamo]
#24013888 - 01/16/17 07:17 AM (7 years, 14 days ago) |
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Quote:
Tiamo said: @AlCapone, I can find cheap PP5 containers in Action, then drill a hole in the side under the lid and put micropore tape on it.
perfect. i like the square ones.

btw, do you notice the irony in having stros how to agar thread linked in your sig?
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AlCapone2k
Beginner


Registered: 10/06/16
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Loc: Germany
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Tiamo]
#24013890 - 01/16/17 07:18 AM (7 years, 14 days ago) |
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Buddy if you have problems opening the sleeve inside the SAB, just lay it on your work desk inside the SAB, cut the sleeve in the middle and get the Petris out from the middle.
The petries inside the sleeve don't have to be pulled out from the top
I'll do it always like this.
I really think this is your problem, that you open the sleeve outside.
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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Tiamo
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: mushboy]
#24013897 - 01/16/17 07:24 AM (7 years, 14 days ago) |
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Unfortunately, I see the irony. I've read just about every thread in this forum yet my results are mediocre to non existent. One day I'll get good like the rest of you. 
@AlCapone Thanks for the tip, I'll try it. Because all my shit contaminates I've only been pouring 5 petris at a time because I don't want to put all my eggs in one basket, so to say. I still have one unopened sleeve left. But I don't want to pour them all. How you you propose storing the half that I don't pour?
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If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
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AlCapone2k
Beginner


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Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Tiamo]
#24013908 - 01/16/17 07:30 AM (7 years, 14 days ago) |
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Just let the unused ones inside the sleeve, roll the sleeve a bit and close it with a food clip
Just try to pour all the petris though, when following the tipps in this thread, you will be sucessful
And try to get petris in a 20-package, then you won't have the problem to open the sleeve inside the SAB
Make a picture of your SAB please
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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NDStepp84
Stellar nuclear waste

Registered: 04/23/15
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Tiamo]
#24013910 - 01/16/17 07:32 AM (7 years, 14 days ago) |
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What size is your SAB? 105 qt or better with big armholes around 5- 6" or more FTW. The tall ones are great, I could g2g half gallons

I kept getting satellite contams and it was driving me nuts, couldn't figure it out. Ended up being my SAB was too small with too small of arm holes.
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"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do. -Robert A. Heinlein
Links and teks ND's grow log and discussion Plant thread
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mushboy
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Registered: 04/24/05
Posts: 32,281
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Tiamo]
#24013913 - 01/16/17 07:34 AM (7 years, 14 days ago) |
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Quote:
Tiamo said: One day I'll get good like the rest of you. 
over 10 years id say its taken me a solid 2years of added up fails. must be. ive fucked up everything so bad. all i can do now is grow them. ive fucked everything possible up to the point where im out of things to fuck up.
now im growing pans and some stone producers. lets see how hard i fail at those first time
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Tiamo
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: NDStepp84]
#24013921 - 01/16/17 07:41 AM (7 years, 14 days ago) |
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This is my SAB. It is 130 L. I use it on a standing desk, and it is very comfortable and easy to move around in and see. I use it like this, with a plastic lid. But I'll use a towel next time.
--------------------
If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
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AlCapone2k
Beginner


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Loc: Germany
Last seen: 6 years, 4 months
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: Tiamo]
#24013934 - 01/16/17 07:49 AM (7 years, 14 days ago) |
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yeah, use a towel and you will get rid of all the problems
Just search for Spitball Jedis SAB Tek and do it like he describes it
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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Mycolorado
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Registered: 07/23/16
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Re: Another run-in with contaminants on agar, where am I going wrong?! [Re: mushboy]
#24013945 - 01/16/17 07:54 AM (7 years, 14 days ago) |
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One thing that stands out to me is the number of time you're touching the plates and moving them around and wiping them. I would suggest you take 5 at a time out in a stack and set them in the box. Spray if you have to. While maintaining the stack, pour into the bottom plate by sandwiching the top plates and the bottom plate lid together so you can crack the bottom plate open 45 degrees and pour in...if that makes sense. Repeat this til you reach the top. This should drastically improve your plate contam rate...not sure why your bottle's contaminating.
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