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OfflineCloneufc
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Reviving master slant!
    #24011081 - 01/14/17 11:24 PM (7 years, 15 days ago)

I still have the Phellinus Linteus master slant dated late 2010. Im hoping I can revive it, so here it goes. I just got back into the medicinals.





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InvisibleEntersandman
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Re: Reviving master slant! [Re: Cloneufc]
    #24011251 - 01/15/17 03:31 AM (7 years, 15 days ago)

you'd better revive the dried tissue in H2O for some weeks until you discover mycelium growth and then transfer it to agar.

i had successes with above method in the last years so you may do the same :awesomenod:

good luck and keep us updated! :super:


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OfflineCloneufc
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Re: Reviving master slant! [Re: Entersandman]
    #24012431 - 01/15/17 03:24 PM (7 years, 15 days ago)

If the agar doesnt revive it,my ziploc bag technique does the trick everytime. I just place it in the bag and wait for the mycelium to get fluffy. It doesnt have a food source, so it goes searching for some and because its in a sterile enviroment,it doesnt contaminate.


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OfflineCloneufc
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Re: Reviving master slant! [Re: Cloneufc]
    #24012436 - 01/15/17 03:27 PM (7 years, 15 days ago)

If that doesnt work, Ill try what you said, sterile water soak.


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OfflineQuadman
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Re: Reviving master slant! [Re: Cloneufc]
    #24012458 - 01/15/17 03:37 PM (7 years, 15 days ago)

I've had luck flipping the wedge upside down and sandwich the mycelium on the agar.


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Edited by Quadman (01/15/17 03:40 PM)


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OfflineFerather
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Re: Reviving master slant! [Re: Cloneufc]
    #24012460 - 01/15/17 03:38 PM (7 years, 15 days ago)

Hydrate and pressure cook some wood toothpicks, cut to size, in some waste vegetable water.*
Stick them into the agar in clean conditions, then remove the pegs when ready.

Transfer to an agar recipe of choice and isolate > verify.

* From making dinner, not from a composter.


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Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

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OfflineCloneufc
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Re: Reviving master slant! [Re: Ferather]
    #24015393 - 01/16/17 05:44 PM (7 years, 14 days ago)

The master slant is contaminated with bacteria :frown:  I still have tissue to work with, next step is to soak tissue in  gentamicin sulphate water, triple rinse with sterile distilled water.Then on to agar with gentamicin sulphate. GS has always worked well for me in the past. I cant do much with this culture until I get the gentamicin sulphate.


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OfflinepoofterFroth
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Re: Reviving master slant! [Re: Cloneufc]
    #24015541 - 01/16/17 06:52 PM (7 years, 14 days ago)

That's an interesting mushroom. Have you grown it out before?

At what concentration will your gentamicin sulphate water be? Is this tissue soaking in gs water a method you've had success with in the past?

:popcorn:


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OfflineFerather
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Re: Reviving master slant! [Re: poofterFroth]
    #24016871 - 01/17/17 09:32 AM (7 years, 13 days ago)

Bacteria you say, Try this, or this. And yes it works. Do no higher than pH 6 or you destabilize the extracts.
You can add calcium carbonate up to pH 6, the calcium can also help fight off infection.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


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OfflineCloneufc
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Re: Reviving master slant! [Re: Ferather]
    #24020733 - 01/18/17 05:25 PM (7 years, 12 days ago)

Yes, I used to grow lots and lots of quart jars of this culture on BRF. I never fruited it but I used to eat the mycelium. Mycelium is better than the fruit body imo. I use 50mg of GS.


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OfflineCloneufc
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Re: Reviving master slant! [Re: Cloneufc]
    #24020746 - 01/18/17 05:30 PM (7 years, 12 days ago)

Sodium Hypochlorite mixed to a concentration of 0.5% to clean bacterial contamination works well.You just add the mixture to a pint jar,PC. After its cooled, you add the contaminated tissue and shake. Let it sit 20 minutes and transfer to a jar of sterile water. Triple rinse. Cleaning up contams on agar is easy, reviving dormant tissue on a contaminated slant, not as easy.


Edited by Cloneufc (01/18/17 05:39 PM)


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