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OfflineNwMycoHead
New mycologist
I'm a teapot User Gallery


Registered: 08/27/16
Posts: 82
Last seen: 6 years, 11 months
My first clone/agar work! and multi-mono grow!!
    #24008766 - 01/14/17 02:54 AM (7 years, 1 month ago)

Let's jump right into it, I started off with these big guys I pulled off my first mono which was Golden Teacher Ms-wbs-cvg in a 27qt mini Monotub...



From there I selected the last guy pictured above ^^ for my cloning project because I liked the structure and it was roughly 40g wet which I thought was a good weight (was immediately placed in paper bag after picture and sealed and placed in fridge overnight to stay fresh)... I then followed pasty's easy agar tek to the letter and prepared some plates. After finished and done cooling I prepared my sab and took a few tissue cultures from the top, middle, and bottom of the inner portion of the stipe. Ending up with this a few days later...

And a few days after that...

I tossed one plate due to not looking as nice as these as well as a small satellite colony starting. So I then prepared some more pasty plates to further isolate the culture...

I allowed them to cool a bit before placing lids and pc'ing as usual to try and help with the condensation (didn't help much) after this I allowed to cool and placed in sab before unwrapping and allowing to sit to let more condensation evaporate. And that is where I'm at with that part of the project now...

  I also have three more 27 qt mini monotubs I just started that go as follows... #1 and #2 are B+ Ms-rye-cvg using 2qts of spawn per tub. One is lined the other is not. This is for my own experimentation (I know what I've read but I want to see results myself) as of today here are those two...

And last but not least is Monotub #3 Golden Teacher Ms-rye-cvg same ratios...


Thanks for checking out my progress, and for helping me get this far. Any feedback or tips is always welcomed and appreciated. And if I posted this in the wrong place or my terminology is off I apologize I'm learning.... hope everyone of you has a great day. (P.s. stoned as shit, took forever to type this)


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Offlinetump
ban the undead
 User Gallery


Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 11 months
Re: My first clone/agar work! and multi-mono grow!! [Re: NwMycoHead]
    #24008771 - 01/14/17 02:59 AM (7 years, 1 month ago)

Oh yea party in the usa. Can't wait for your 60 tubs of iso culture testing

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OfflineNwMycoHead
New mycologist
I'm a teapot User Gallery


Registered: 08/27/16
Posts: 82
Last seen: 6 years, 11 months
Re: My first clone/agar work! and multi-mono grow!! [Re: tump]
    #24009494 - 01/14/17 10:52 AM (7 years, 1 month ago)

Quote:

tump said:
Oh yea party in the usa. Can't wait for your 60 tubs of iso culture testing



60 tubs? Maybe 60 pasty plates but if I had 60 tubs IDK what I'd do with all that fruit. Haha. Thanks for checking this out.


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Offlinetump
ban the undead
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Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 11 months
Re: My first clone/agar work! and multi-mono grow!! [Re: NwMycoHead]
    #24010776 - 01/14/17 08:29 PM (7 years, 1 month ago)

You got to make Atleast one tub per plate for testing the culture

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OfflineCapnZ
Dimensional explorer
Male


Registered: 12/22/16
Posts: 1,420
Loc: In the Mystic
Last seen: 4 months, 14 days
Re: My first clone/agar work! and multi-mono grow!! [Re: tump]
    #24010872 - 01/14/17 09:18 PM (7 years, 1 month ago)

Those agar plates look pretty damn nice for the 1st time out. Hope I get similar results!!


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Deep into the darkness peering, long I stood there wondering, fearing, doubting, dreaming dreams no mortal ever dared to dream before...

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Offlineprojectbadass
Wrangler


Registered: 12/20/16
Posts: 100
Loc: Northeast Ohio Flag
Last seen: 2 years, 8 months
Re: My first clone/agar work! and multi-mono grow!! [Re: CapnZ]
    #24010881 - 01/14/17 09:24 PM (7 years, 1 month ago)

:billymaythumbup:


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OfflineNwMycoHead
New mycologist
I'm a teapot User Gallery


Registered: 08/27/16
Posts: 82
Last seen: 6 years, 11 months
Re: My first clone/agar work! and multi-mono grow!! [Re: projectbadass]
    #24010906 - 01/14/17 09:36 PM (7 years, 1 month ago)

Quote:

tump said:
You got to make Atleast one tub per plate for testing the culture




Well true that, but I don't think I'll need 60 maybe 10 only gonna keep the best plates and test though from there... just having fun with it.



Quote:

CapnZ said:
Those agar plates look pretty damn nice for the 1st time out. Hope I get similar results!!




Quote:

projectbadass said:
:billymaythumbup:




Thanks guys, appreciate it.


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