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OfflineBaconator
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Registered: 08/28/16
Posts: 5
Last seen: 5 years, 10 months
ID request
    #24009576 - 01/14/17 11:23 AM (7 years, 16 days ago)

Habitat:
Bayy area in woodchips by a shopping center.

Gills:
Brownish. Not completely sure about whether they are connected or not. They seem old so picture of gills included.

Stem:
Long, white, sturdy but hollow. No blue bruising.

Cap:
Medium size, wavy, whitish tan.

Spore print color:
Black

Bruising:
Color that the mushroom bruises, if any.

Other information:


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OfflineBaconator
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Registered: 08/28/16
Posts: 5
Last seen: 5 years, 10 months
Re: ID request [Re: Baconator]
    #24009584 - 01/14/17 11:25 AM (7 years, 16 days ago)



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Offlineleschampignons
Biochemistry + Mycology
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Registered: 08/30/13
Posts: 1,583
Loc: NY/NJ/ME Flag
Last seen: 4 days, 8 hours
Re: ID request [Re: Baconator]
    #24009602 - 01/14/17 11:32 AM (7 years, 16 days ago)

Kind of looks like a Stropharia sp.


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OfflineAlan RockefellerM
Mycologist
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Registered: 03/10/07
Posts: 48,276
Last seen: 5 hours
Trusted Identifier
Re: ID request [Re: leschampignons]
    #24009646 - 01/14/17 11:57 AM (7 years, 16 days ago)

Leratiomyces riparius

I got an ITS sequence from a wood chip collection from the Santz Cruz police station but it was really odd and the closest match was 85%.  I need to find the frozen DNA tube and retry the PCR with LSU primers.


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Offlineleschampignons
Biochemistry + Mycology
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Registered: 08/30/13
Posts: 1,583
Loc: NY/NJ/ME Flag
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Re: ID request [Re: Alan Rockefeller]
    #24010682 - 01/14/17 07:54 PM (7 years, 16 days ago)

Where do you buy your primers Alan?


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OfflineAlan RockefellerM
Mycologist
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Registered: 03/10/07
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Re: ID request [Re: leschampignons]
    #24019874 - 01/18/17 12:45 PM (7 years, 12 days ago)

Quote:

leschampignons said:
Where do you buy your primers Alan?




IDT.    Costs about $7 for enough for 2500 PCR reactions.

The master mix I get from ebay, costs about 25 cents per reaction.

Here is a web page I wrote with sources and details and procedures:  https://counterculturelabs.org/wiki/DNA_sequencing


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Offlineleschampignons
Biochemistry + Mycology
Male User Gallery

Registered: 08/30/13
Posts: 1,583
Loc: NY/NJ/ME Flag
Last seen: 4 days, 8 hours
Re: ID request [Re: Alan Rockefeller]
    #24020468 - 01/18/17 03:50 PM (7 years, 12 days ago)

:toast: thanks


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