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OfflineOrbit
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Registered: 12/06/16
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Re: Agar issues [Re: Killerclowns87]
    #24018498 - 01/17/17 08:41 PM (7 years, 13 days ago)

I think it depends what side is warmer. I do alot of yeast culturing and (storage )in the fridge they are real bad for moisture on the lid if right side up, beer fridge so cooling is comming from above. My incubator has heat at the bottom. So moisture acts the same moving upward.

Try both ways and see witch works better for you. My current conditions upsidedown works best.


Not sure what RR is talking about hear. "cold material can hold less moisture than warm material"?

Its opposite cold material attracts moisture and warm releases it.
Boiling pot vs ice cold window where is the moisture going?



Wikipedia

Petri plates are incubated upside-down to lessen the risk of contamination from airborne particles settling on them and to prevent the accumulation of any water condensation that may otherwise disturb or compromise a culture.


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InvisibleMycolorado
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Registered: 07/23/16
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Re: Agar issues [Re: Orbit]
    #24018569 - 01/17/17 09:19 PM (7 years, 13 days ago)



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Offlinestromam
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Re: Agar issues [Re: Intelligentxfruit]
    #27580406 - 12/13/21 07:13 PM (2 years, 1 month ago)

Quote:

Intelligentxfruit said:
Does the puddle form immediately/after a couple hours? Or does it take 1-3days to show




I'm sorry to revive a fairly old post, but I've been having this exact problem with a large number of my plates. I think I might have an idea.  Could it be cold agar/tissue creating condensation on itself?  I've seen this problem from both agar to agar and tissue cultures.  I didn't take notes on the temperatures of the samples I was transferring from, but to the best of my recollection all the plates that have puddled like this came from wedges or mushrooms that had recently been refrigerated.  Here are some pictures.





This last one is the only one of about 10 different plates I made/transferred yesterday that didn't puddle overnight. It came from a pretty old (1 year+) and pretty dry looking plate, so perhaps the condensation it collected immediately got resorbed into the dry wedge.



My "puddle wedges" have always grown out just fine with no obvious contamination, other than the mycelium seems to follow the drop.  By that I mean if the drop doesn't stay put when I pick up a dish, but slides around on it plate it leaves a "snail trail" of mycelium spawn points.

If that's the cause I guess I need to let my samples come to room temp over a couple hours before making my transfers.  Maybe the more experienced are doing this already.


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