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Offlineukshroomer
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strain isolation question * 1
    #24007387 - 01/13/17 02:39 PM (7 years, 17 days ago)

Hello. I have made several petri dishes. These petri dishes are one transfer from the initial multispore swipe dish. I am trying to get isolates in the near future. I have a few questions and it would be great if someone with agar isolation experience could help.

1. How large should should i let the mycelium grow before taking new transfers. My dishes are 5.5 cm in diameter. ( I cannot see any sectoring yet as this is the first transfer from multispore).

2. I have 4 dishes of this strain (B+). How many transfers should I make from each dish(1,2, 3 or 4??). I have a lot of petri dishes so that is not the issue.

3. For those who have experience: How many transfers does it usually take you before you get to single sector isolate and how many transfers before i should see sectoring?


Please let me know. Have a nice weekend.



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InvisibleAlgo
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Re: strain isolation question [Re: ukshroomer]
    #24007403 - 01/13/17 02:44 PM (7 years, 17 days ago)

I am no expert, but I think you should clone a quality fruit and then try that shit. Spores give you way to many strains.


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InvisiblemushboyMDiscord
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Re: strain isolation question [Re: Algo]
    #24007409 - 01/13/17 02:46 PM (7 years, 17 days ago)

:whathesaid:

otherwise you can isolate a non fruiter. or bunk:sad:


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Offlineukshroomer
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Re: strain isolation question [Re: Algo]
    #24007420 - 01/13/17 02:48 PM (7 years, 17 days ago)

I actually really would like to try isolation. A clone can also contain more than one strain plus there is the risk of senescence after using it for a while. I am willing to test 30 or 40 isolates.


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Edited by ukshroomer (01/13/17 02:49 PM)


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InvisibleAlgo
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Re: strain isolation question [Re: ukshroomer]
    #24007433 - 01/13/17 02:52 PM (7 years, 17 days ago)

So if you mix a bunch of spores how are you gonna know if what you isolate is good? When you clone you can take a quality fruit, then make culture slants and use the same strain for years and years.


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InvisibleAlgo
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Re: strain isolation question [Re: Algo]
    #24007437 - 01/13/17 02:53 PM (7 years, 17 days ago)

Just like mushboy said, what if you isolate a pile turds that aint worth shit?


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InvisibleAlgo
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Re: strain isolation question [Re: Algo]
    #24007453 - 01/13/17 02:57 PM (7 years, 17 days ago)

It seems to me that testing 30-40 isolates is a waste of time, but that's just my opinion.


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InvisibleMycolorado
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Re: strain isolation question [Re: Algo] * 1
    #24007494 - 01/13/17 03:11 PM (7 years, 17 days ago)

There's more to the hobby than just growing mushrooms.  Believe it or not, some people actually enjoy culture work for the sake of culture work.


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OfflinecronicrFacebook
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Re: strain isolation question [Re: Mycolorado]
    #24007509 - 01/13/17 03:16 PM (7 years, 17 days ago)

Quote:

Algo said:
It seems to me that testing 30-40 isolates is a waste of time, but that's just my opinion.



pheewww glad it's only your opinion:crazy:
Quote:

Mycolorado said:
There's more to the hobby than just growing mushrooms.  Believe it or not, some people actually enjoy culture work for the sake of culture work.



:fuckinawesome: i got cultures of shit that i will never grow or even stand a chance of....lol


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Offlineukshroomer
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Re: strain isolation question [Re: cronicr]
    #24007513 - 01/13/17 03:18 PM (7 years, 17 days ago)

Cumon guys lets get some serious answers lol


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InvisibleAlgo
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Re: strain isolation question [Re: Mycolorado]
    #24007518 - 01/13/17 03:19 PM (7 years, 17 days ago)

Waste your time getting nowhere I don't give a fuck I was just telling him the most efficient way to make quality shit.


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OfflinecronicrFacebook
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Re: strain isolation question [Re: ukshroomer]
    #24007528 - 01/13/17 03:21 PM (7 years, 17 days ago)

take as many transfers as you want from each plate i take a few from any good looking growth but i dont get too picky till after about 7 transfers when i see good sectoring, an iso can take 12 it could take up to 20 ya never know, do lots of plates lots of testing and get slants ready, algo you go right ahead with shit advise and see if anybody gives a fuck i sure dont:stonedjerk:


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Offlineukshroomer
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Re: strain isolation question [Re: cronicr]
    #24007536 - 01/13/17 03:25 PM (7 years, 17 days ago)

Thanks cronicr. I am already seeing a little bit of rhizo growth on on of the B+ plates :smile: I am gonna transfer it tomorrow.


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OfflinecronicrFacebook
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Re: strain isolation question [Re: ukshroomer]
    #24007549 - 01/13/17 03:28 PM (7 years, 17 days ago)

Nice! the great thing about it is you will have tons of plates and can still get some good ms grows going while you keep at it


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InvisibleAlgo
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Re: strain isolation question [Re: cronicr]
    #24007552 - 01/13/17 03:28 PM (7 years, 17 days ago)

My advice isn't shit, it's efficient if you are trying to get somewhere with quality fruits. If you just wanna keep making cultures for the sake of making cultures then go ahead do it's fun, I just wanted the fella to get quality fruits as quickly as possible.


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OfflinecronicrFacebook
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Re: strain isolation question [Re: Algo]
    #24007553 - 01/13/17 03:29 PM (7 years, 17 days ago)

i'm not saying your wrong but telling someone testing a bunch of iso's is a waiste of time is total bs, clones need testing too and have the same odds as any iso with more cell division


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I'm tired do me a favor


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Offlineenlightenment
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Re: strain isolation question [Re: cronicr]
    #24007564 - 01/13/17 03:33 PM (7 years, 17 days ago)

Quote:

3. For those who have experience: How many transfers does it usually take you before you get to single sector isolate and how many transfers before i should see sectoring?




Sometimes you can see different sectors on the first dish.



Anyway as cronicr said you need more transfers to get an isolate.
A good isolate is nice to have, it takes time, resources and some effort.
Maybe its all for nothing if the isolate turns out to not fruit at all.


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InvisibleJosex
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Re: strain isolation question [Re: ukshroomer]
    #24007568 - 01/13/17 03:35 PM (7 years, 17 days ago)

1. Those plates are ready to take transfers from.
2. If you have more projects than just strain isolation (you def should) you should take at least 2 or 3 transfers from the leading edges just to expand the myc. But if your only purpose is strain isolation i would not take more than one transfer from those (well, maybe two, so you can choose between the 2).
IMO you should only take more than one transfer once you start seeing clear sectoring and that can take 10 transfers or more (that answers the question 3).

Imagine that you only take one transfer from those 4 plates until you start seeing sectoring, so now, after x tranfers you have 4 plates, each showing lets say 10 sectors, so now it's when you should start isolating all those 40 sectors and that's when shit can get real crazy and out of hand.


Edited by Josex (01/13/17 03:42 PM)


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OfflinecronicrFacebook
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Re: strain isolation question [Re: Josex]
    #24007575 - 01/13/17 03:37 PM (7 years, 17 days ago)

it's gonna get crazy n out of hand no matter what.....thats just the way she goes:lol:


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It doesn't matter what i think of you...all that matters is clean spawn

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Offlineukshroomer
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Re: strain isolation question [Re: Algo]
    #24007577 - 01/13/17 03:37 PM (7 years, 17 days ago)

To algo. I already know how to get lots of fruits. I am looking for a way to get the exact same results every time. Clones can sector. This means that when you take mycelium from a slant you might not get the same results you did last time.


Edited by ukshroomer (01/13/17 03:38 PM)


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