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Invisibleberlinmyc
I'm a teapot

Registered: 12/21/15
Posts: 98
Culture Questions
    #24007084 - 01/13/17 12:43 PM (7 years, 17 days ago)

Hey good people. I have 3 agar plates. They are the second transfer from GT mulitispore. I'm trying to decide which community is the healthiest so I can practice fungal eugenics. If you could grant me the benefits of your experience I would be most appreciative.

A.


B. This one appears to be the best imo


C. Weak growth and near a contaminant

Which culture do you think is the best and how many sectors do you believe they contain. A couple dozen or more? That is at least what my virgin eyes perceive.


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Invisiblenatedawgnow
Rocky mountain hood rat
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Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: Culture Questions [Re: berlinmyc]
    #24007097 - 01/13/17 12:47 PM (7 years, 17 days ago)

On plate 1 I would take from around 10 o'clock

Plate 2 looks good and you can take from basically any sector that seems
to be growing faster than the surrounding sector.

Plate 3 transfer from the opposite side of the contamination, so I would
say anywhere from 12-2.


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OfflineMattisfat
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Registered: 01/20/16
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Re: Culture Questions [Re: natedawgnow]
    #24007122 - 01/13/17 12:59 PM (7 years, 17 days ago)

are those bubbles in your agar or did they appear after inoculation?


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Invisibleberlinmyc
I'm a teapot

Registered: 12/21/15
Posts: 98
Re: Culture Questions [Re: Mattisfat]
    #24008402 - 01/13/17 10:50 PM (7 years, 17 days ago)

Thanks for the tips.
Quote:

Mattisfat said:
are those bubbles in your agar or did they appear after inoculation?



The bubbles solidified in the agar. I was experimenting with additives and the mix got a bit chunky as you can see on slide C.


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InvisiblemushboyMDiscord
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Re: Culture Questions [Re: berlinmyc]
    #24008410 - 01/13/17 10:57 PM (7 years, 17 days ago)

Id transfer from 10 oclock also on #1. or maybe 3 o clock. up to you.

the 2nd plate looks nice. id reserve a cut but use the plate for some BI
to get you some quickly colonized jars so you can get some clones quickly if yah want.

i have no clue about sectors. im still focusing on cleaning up my cultures.


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InvisibleMycolorado
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Re: Culture Questions [Re: berlinmyc]
    #24008435 - 01/13/17 11:08 PM (7 years, 17 days ago)

Those look great!  I see similar growth on DFA.  What's that agar recipe?


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OfflineOrbit
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Re: Culture Questions [Re: mushboy]
    #24008445 - 01/13/17 11:13 PM (7 years, 17 days ago)

Look for rizomorphic growth the stuff that is more string like and reaching. C at 12-1:00 looks nice. Do a few to agar. And jars as well.


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Invisibleberlinmyc
I'm a teapot

Registered: 12/21/15
Posts: 98
Re: Culture Questions [Re: Orbit]
    #24008682 - 01/14/17 01:41 AM (7 years, 16 days ago)

Quote:

Mycolorado said:
Those look great!  I see similar growth on DFA.  What's that agar recipe?



Potato dextrose, agar, gypsum, yeast, honey

Quote:

Orbit said:
Look for rizomorphic growth the stuff that is more string like and reaching. C at 12-1:00 looks nice. Do a few to agar. And jars as well.



I already have a bunch of jars plus a colonizing monotub


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InvisibleMycolorado
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Re: Culture Questions [Re: berlinmyc]
    #24009141 - 01/14/17 08:32 AM (7 years, 16 days ago)

That's quite the blend.  I don't like PDA but the additives you've used sure made a nice medium.  Anyway, all of those cultures are nice and uniform so you could transfer from anywhere.  Still tons of sectors in those.  Nice work, keep it up!


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OfflineDactylium
Don't touch me I'm sterile


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Re: Culture Questions [Re: berlinmyc]
    #24009391 - 01/14/17 10:15 AM (7 years, 16 days ago)

:paradis: good work dude


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Invisibleberlinmyc
I'm a teapot

Registered: 12/21/15
Posts: 98
Re: Culture Questions [Re: Dactylium]
    #24009694 - 01/14/17 12:24 PM (7 years, 16 days ago)

Quote:

Mycolorado said:
That's quite the blend.  I don't like PDA but the additives you've used sure made a nice medium.  Anyway, all of those cultures are nice and uniform so you could transfer from anywhere.  Still tons of sectors in those.  Nice work, keep it up!



Thanks, man. I switched up the medium today. Potato dextrose, LME, gypsum, and honey. Makin gravy!

Quote:

Dactylium said:
:paradis: good work dude



Thanks, dude.


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Invisibleberlinmyc
I'm a teapot

Registered: 12/21/15
Posts: 98
Re: Culture Questions [Re: berlinmyc]
    #24009759 - 01/14/17 12:51 PM (7 years, 16 days ago)

Hey so since I already made a post I have another question about my mono. I'm trying to figure out if If what I'm seeing is grey cobweb or if i'm seeing the substrate through fuzzy Mycelium.

Overview:




This must be cobweb right?


Also should I dab heavy condensation?


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OfflineDactylium
Don't touch me I'm sterile


Registered: 08/12/16
Posts: 696
Last seen: 10 months, 13 days
Re: Culture Questions [Re: berlinmyc]
    #24009855 - 01/14/17 01:49 PM (7 years, 16 days ago)

Not cobweb but it looks ill


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Invisibleberlinmyc
I'm a teapot

Registered: 12/21/15
Posts: 98
Re: Culture Questions [Re: Dactylium]
    #24010260 - 01/14/17 04:35 PM (7 years, 16 days ago)

Dang


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InvisibleMycolorado
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Re: Culture Questions [Re: berlinmyc]
    #24010318 - 01/14/17 05:12 PM (7 years, 16 days ago)

That thick bright white growth is troubling.  I don't want to say it's mold but it sure has a distinct morphology if it's cube mycelium.


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