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Walkingbio
Mushie Lives Matter


Registered: 03/03/17
Posts: 39
Loc: NC
Last seen: 1 year, 5 months
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Re: A Grow Log, An Idiot Tub, Some Nerd Crap... And A Question [Re: lovelaughlibs]
#24138942 - 03/05/17 02:41 PM (6 years, 10 months ago) |
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Quote:
lovelaughlibs said:
Quote:
Walkingbio said: Fucking hilarious lol I haven't even started my first grow yet I'm nervous about getting a contamination the central air filters are DISGUSTING in my house. I don't have the money to replace them right now so I figured I'll do the inoculation in a still air box that has been sterilized. I'm going to cover the vent in my room with a wet towel and then spray down the bedroom and closest focusing more on the closet, then hopefully get a clean spawn bag out of it. Still scared, I won't be using jars just one big bag of grain good chance to mess up everything for me./:
Don't worry too much, the air is utterly filthy almost everywhere on the planet. It comes down to technique - you don't need to sterilise the SAB, just spray the walls with some lightly soaped water and work at a reasonably quick speed without stirring up air currents.
You know to sterilise bags for a lot longer than jars also, right? The heat takes longer to penetrate all the way to the middle of the grains. I've heard 3 hours is a good time but I'm not an expert.
Do you think misting alcohol on the walls would sterilize? Or is the soapy water used to catch spores or other contaminants on the wall? If so I'll try that, also I haven't tried making my own bags or jars yet. I bought them from a what looks and heard to be a reputable supplier of sterile goods. The bags have ships as well, when I can afford a pressure cooker I'll be better off.
-------------------- "It's the moment you face your fears, you realize you have the strength and desire to keep fighting." - Walkingbio
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Collector
Cultivator



Registered: 02/19/17
Posts: 145
Loc: Canada
Last seen: 6 years, 8 months
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Re: A Grow Log, An Idiot Tub, Some Nerd Crap... And A Question [Re: Walkingbio]
#24138953 - 03/05/17 02:47 PM (6 years, 10 months ago) |
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I dont recommend spraying alcohol around in an enclosed space, even if youre not using a flame to sterilize. I once had a friend blow his door off the hinges when his mom walked by his sterilized lab and lit a cigarette.
-------------------- Happiness is when: What the world needs, meets, what you have to offer Hi. Im the character, Spagett and when im not spooking, I like to smoke.
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Walkingbio
Mushie Lives Matter


Registered: 03/03/17
Posts: 39
Loc: NC
Last seen: 1 year, 5 months
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Re: A Grow Log, An Idiot Tub, Some Nerd Crap... And A Question [Re: Collector]
#24138985 - 03/05/17 02:59 PM (6 years, 10 months ago) |
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I wouldn't spray it in there and light a flame next to it right away. Honestly I would give the alcohol and air time to settle, if air is moving around in there then it wouldn't be a SAB right?
-------------------- "It's the moment you face your fears, you realize you have the strength and desire to keep fighting." - Walkingbio
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Shameful Idiot
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Registered: 01/12/17
Posts: 65
Last seen: 3 years, 8 months
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Re: A Grow Log, An Idiot Tub, Some Nerd Crap... And A Question [Re: Walkingbio]
#24139096 - 03/05/17 03:44 PM (6 years, 10 months ago) |
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Quote:
Walkingbio said: Fucking hilarious lol I haven't even started my first grow yet I'm nervous about getting a contamination the central air filters are DISGUSTING in my house. I don't have the money to replace them right now so I figured I'll do the inoculation in a still air box that has been sterilized. I'm going to cover the vent in my room with a wet towel and then spray down the bedroom and closest focusing more on the closet, then hopefully get a clean spawn bag out of it. Still scared, I won't be using jars just one big bag of grain good chance to mess up everything for me./:
People have said this already but REALLY don't worry about it. I live in a super old house, I scoff at the concept of air movement even having a filter (right now it's three fan heaters), and I don't have a SAB or flow hood. I just turn off the heater in the living room about 20 minutes before I start working and use real, real good sterile procedure. I've had some mishaps but no more than anyone else has I think. And what mishaps I've had were from being an imbecile (setting the needle down on the couch after sterilizing it), nothing to do with my air.
And this is with a cat wandering through my work area regularly (I mean I yell at him but y'know.)
You can spray some peroxide on stuff too, it's an oxidizing agent but it's not as openly flammable as iso.
When I "refresh" an old fruiting chamber these days I run the perlite full of water until it's a slurry, pour a bit of bleach in, swish it around, and let it soak and drain for an hour before doing the same with fresh water. I also spray peroxide on the sides before I first put the cakes in (I leave it open for half an hour afterwards to let the verm adhere a bit before I spray it.) I mean it's probably overkill, but if you're gonna do this, why fuck around, right?
Just do keep them separate. My water sprayers are 750mL ones from the garden store, my peroxide is a 500mL sprayer from Walmart that looks way different.
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Shameful Idiot
Stranger
Registered: 01/12/17
Posts: 65
Last seen: 3 years, 8 months
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Re: A Grow Log, An Idiot Tub, Some Nerd Crap... And A Question [Re: Shameful Idiot]
#24139128 - 03/05/17 03:53 PM (6 years, 10 months ago) |
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Oh, additionally, since I'm not sure when my bags are arriving, I decided to see if a heavy duty ziploc freezer bag would survive the PC. Being a smart person who doesn't want to ruin their PC, I stuffed it in an old pretty-much-empty WBS jar and put it in for 20 minutes.
Tip: probably don't do this.
Edited by Shameful Idiot (03/05/17 10:35 PM)
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Walkingbio
Mushie Lives Matter


Registered: 03/03/17
Posts: 39
Loc: NC
Last seen: 1 year, 5 months
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Re: A Grow Log, An Idiot Tub, Some Nerd Crap... And A Question [Re: lovelaughlibs]
#24140876 - 03/06/17 10:44 AM (6 years, 10 months ago) |
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Quote:
lovelaughlibs said:
Quote:
Walkingbio said: Fucking hilarious lol I haven't even started my first grow yet I'm nervous about getting a contamination the central air filters are DISGUSTING in my house. I don't have the money to replace them right now so I figured I'll do the inoculation in a still air box that has been sterilized. I'm going to cover the vent in my room with a wet towel and then spray down the bedroom and closest focusing more on the closet, then hopefully get a clean spawn bag out of it. Still scared, I won't be using jars just one big bag of grain good chance to mess up everything for me./:
Don't worry too much, the air is utterly filthy almost everywhere on the planet. It comes down to technique - you don't need to sterilise the SAB, just spray the walls with some lightly soaped water and work at a reasonably quick speed without stirring up air currents.
You know to sterilise bags for a lot longer than jars also, right? The heat takes longer to penetrate all the way to the middle of the grains. I've heard 3 hours is a good time but I'm not an expert.
Would spic and span did the trick?
-------------------- "It's the moment you face your fears, you realize you have the strength and desire to keep fighting." - Walkingbio
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Shameful Idiot
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Last seen: 3 years, 8 months
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Re: A Grow Log, An Idiot Tub, Some Nerd Crap... And A Question [Re: Walkingbio]
#24142537 - 03/06/17 10:42 PM (6 years, 10 months ago) |
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Er... maybe? But not necessarily? Honestly peroxide is cheap and sprayers are cheaper.
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Walkingbio
Mushie Lives Matter


Registered: 03/03/17
Posts: 39
Loc: NC
Last seen: 1 year, 5 months
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Re: A Grow Log, An Idiot Tub, Some Nerd Crap... And A Question [Re: Shameful Idiot]
#24142544 - 03/06/17 10:47 PM (6 years, 10 months ago) |
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It's already done now P: my girl got me some Lysol and rubber gloves. I was upset with the gauge of the needle on that syringe. Damn thing was so wide it took a chunk out of each SHIP.
-------------------- "It's the moment you face your fears, you realize you have the strength and desire to keep fighting." - Walkingbio
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Mr Piggy
Big Dick Retard



Registered: 09/29/11
Posts: 8,401
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Re: A Grow Log, An Idiot Tub, Some Nerd Crap... And A Question [Re: Shameful Idiot]
#24142546 - 03/06/17 10:48 PM (6 years, 10 months ago) |
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Quote:
Shameful Idiot said: Honestly peroxide is cheap and sprayers are cheaper.
It also does nothing for sanitation.
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Walkingbio
Mushie Lives Matter


Registered: 03/03/17
Posts: 39
Loc: NC
Last seen: 1 year, 5 months
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Re: A Grow Log, An Idiot Tub, Some Nerd Crap... And A Question [Re: Mr Piggy]
#24142565 - 03/06/17 10:55 PM (6 years, 10 months ago) |
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Doesn't it kill bacteria?
-------------------- "It's the moment you face your fears, you realize you have the strength and desire to keep fighting." - Walkingbio
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Shameful Idiot
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Registered: 01/12/17
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Last seen: 3 years, 8 months
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Re: A Grow Log, An Idiot Tub, Some Nerd Crap... And A Question [Re: Walkingbio]
#24142627 - 03/06/17 11:44 PM (6 years, 10 months ago) |
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You sure as hell won't see a TC tag next to my name but I mean I'm presuming my friend's commercial mushroom farm that uses peroxide as a sanitizing soak and spray (along with iso for some tasks) isn't just staffed by complete imbeciles.
And that the cobweb I annihilated with it yesterday actually just committed suicide when it realized I didn't like it.
That said, it's very true that not every product kills spores as well as bacteria, so you have to make sure you're using an appropriate product for what you're trying to accomplish.
Edited by Shameful Idiot (03/06/17 11:46 PM)
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Mr Piggy
Big Dick Retard



Registered: 09/29/11
Posts: 8,401
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Re: A Grow Log, An Idiot Tub, Some Nerd Crap... And A Question [Re: Walkingbio]
#24143371 - 03/07/17 10:02 AM (6 years, 10 months ago) |
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Quote:
Walkingbio said: Doesn't it kill bacteria?
Not at any kind of rate to be useful.
It's like libs said, focus on SAB technique.
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Edited by Mr Piggy (03/07/17 10:05 AM)
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Mr Piggy
Big Dick Retard



Registered: 09/29/11
Posts: 8,401
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Re: A Grow Log, An Idiot Tub, Some Nerd Crap... And A Question [Re: Shameful Idiot]
#24143400 - 03/07/17 10:11 AM (6 years, 10 months ago) |
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Quote:
Shameful Idiot said:
That said, it's very true that not every product kills spores as well as bacteria, so you have to make sure you're using an appropriate product for what you're trying to accomplish.
Focus on your technique more than "products"
I see on the last page you went straight spore syringe to grain. That's a great way to get contaminates. The experts on here hammer home again and again that SAB technique and clean spawn are what we should focus on.
Following the advice of TCs on here is going great for me. Only works if you follow them word for word, step for step.
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Walkingbio
Mushie Lives Matter


Registered: 03/03/17
Posts: 39
Loc: NC
Last seen: 1 year, 5 months
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Re: A Grow Log, An Idiot Tub, Some Nerd Crap... And A Question [Re: Mr Piggy]
#24143525 - 03/07/17 11:08 AM (6 years, 10 months ago) |
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So I sprayed my closest and SAB with lysol ,did my inoculation naked as well as tried to slow breathing an physical movement to restrict air movement. I wiped the inoculation port with alcohol as I heated the syringe, cooled the syringe with alcohol and immediately injected. With you guy's experience would you guys recommend more precaution? If so would you share the methods with me?
-------------------- "It's the moment you face your fears, you realize you have the strength and desire to keep fighting." - Walkingbio
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Mr Piggy
Big Dick Retard



Registered: 09/29/11
Posts: 8,401
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Re: A Grow Log, An Idiot Tub, Some Nerd Crap... And A Question [Re: Walkingbio]
#24143777 - 03/07/17 12:50 PM (6 years, 10 months ago) |
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Quote:
Walkingbio said: cooled the syringe with alcohol and immediately injected.
This is a mistake and introduces contaminates. Cool the needle by squirting out a couple of drops.
You also don't need to be naked, just shower and wear clean clothes.
Are you inoculating grain with spores, or BRF cakes?
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Shameful Idiot
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Re: A Grow Log, An Idiot Tub, Some Nerd Crap... And A Question [Re: Mr Piggy]
#24144269 - 03/07/17 04:19 PM (6 years, 10 months ago) |
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Quote:
Mr Piggy said:
Quote:
Walkingbio said: cooled the syringe with alcohol and immediately injected.
This is a mistake and introduces contaminates. Cool the needle by squirting out a couple of drops.
You also don't need to be naked, just shower and wear clean clothes.
Are you inoculating grain with spores, or BRF cakes?
Appreciate your earlier comments too, don't get me wrong, I in no way WANTED to go spores to grain, but I didn't have anything else to inoculate it with at the time (LCs aren't even close to being ready.)
Another fine reason not to inoculate immediately after you're flamed the needle is that depending how fast you're doing it you might be killing half the spores that go through the hot needle too. Easy enough to let it cool in the air a couple seconds, put a few drops through, and if you're still worried wipe it down with some alcohol but there's probably no need. You can spare a few drops of spore solution and there's only so much you want to go wiping things on a needle you just sterilized ("ideally nothing").
And yeah being naked is overkill. The important part is to not have debris from your face (hair, eyebrows, moustache if you have one) fall on your work surface, or that you don't breathe contaminants onto it. The mushrooms, I've found, don't really care if your dick's out...
Edited by Shameful Idiot (03/07/17 04:24 PM)
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Mr Piggy
Big Dick Retard



Registered: 09/29/11
Posts: 8,401
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Re: A Grow Log, An Idiot Tub, Some Nerd Crap... And A Question [Re: Shameful Idiot]
#24144386 - 03/07/17 05:04 PM (6 years, 10 months ago) |
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Quote:
Shameful Idiot said: Easy enough to let it cool in the air a couple seconds, put a few drops through, and if you're still worried wipe it down with some alcohol but there's probably no need.
Do not wipe with alcohol after flaming. It introduces contaminates.
I cool it with a drop immediately after flaming and have had zero germination issues.
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Collector
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Registered: 02/19/17
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Loc: Canada
Last seen: 6 years, 8 months
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Re: A Grow Log, An Idiot Tub, Some Nerd Crap... And A Question [Re: Mr Piggy]
#24144442 - 03/07/17 05:34 PM (6 years, 10 months ago) |
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I agree with Mr. Piggy, theres no need to re wipe after you sterilize.
-------------------- Happiness is when: What the world needs, meets, what you have to offer Hi. Im the character, Spagett and when im not spooking, I like to smoke.
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Shameful Idiot
Stranger
Registered: 01/12/17
Posts: 65
Last seen: 3 years, 8 months
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Re: A Grow Log, An Idiot Tub, Some Nerd Crap... And A Question [Re: Mr Piggy]
#24144562 - 03/07/17 06:21 PM (6 years, 10 months ago) |
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Quote:
Mr Piggy said:
Quote:
Shameful Idiot said: Easy enough to let it cool in the air a couple seconds, put a few drops through, and if you're still worried wipe it down with some alcohol but there's probably no need.
Do not wipe with alcohol after flaming. It introduces contaminates.
I cool it with a drop immediately after flaming and have had zero germination issues.
Yeah I give it a shake up a bit (to distribute the spores around), which generally gets a little bit of liquid into the needle, then just squeeze out a few drops above my alcohol towel. Above. Not on. Mr. Piggy's right, if alcohol was good enough to sterilize it, we wouldn't need the flame.
90-100% of contamination issues I've had at the germination stage (which thankfully, after the first batch, was very very few) went in with the needle (as opposed to already being in the substrate / contaminating from the air afterwards), so getting that part right is important.
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Shameful Idiot
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Re: A Grow Log, An Idiot Tub, Some Nerd Crap... And A Question [Re: Shameful Idiot]
#24147168 - 03/08/17 07:09 PM (6 years, 10 months ago) |
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Well apparently I did a bad job with THAT spore syringe, I now have ten jars with the beginnings of very healthy trich colonies.
It's small enough on some of them that I'm waffling between dumping the substrate and just throwing them back in the PC. Thoughts? Perfectly fine idea? Not even close to enough to kill all the spores? Literally the dumbest thing you've ever heard?
Also since I have absolutely no desire to do spores-to-grain again but I do need a bunch more grain jars and the previous batch of transfers haven't finished yet... what can I throw in grain? I mean stem pieces would be a bit cheeky, but chunks of living mycelium from BRF cakes? I dunno. Or I could just wait a few days for my other grain jars to be ready to transfer.
EDIT: Speaking of stem pieces, my neighbor was over a couple weeks ago and picked a couple fresh fruits for a treat at home - he ended up not having time that evening I guess, so he put some stem chunks into a plastic freezer bag with un-pasteurized straw and sawdust. Apparently it's now fully colonize and free of contaminants. Lucky bastard. I'll see if I can get him to bring it over for a picture.
Edited by Shameful Idiot (03/08/17 09:42 PM)
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