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OfflineShameful Idiot
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Registered: 01/12/17
Posts: 65
Last seen: 3 years, 8 months
Re: A Grow Log, An Idiot Tub, Some Nerd Crap... And A Question [Re: Newbie McShroomie]
    #24112148 - 02/22/17 09:43 PM (6 years, 11 months ago)

Quote:

Newbie McShroomie said:
Im sorry Shameful, but you are no Idiot... I am totally stealing your barcode idea. You hit the whole concept of knowing every little detail SPOT ON IMO.

And so you have already spawned to bulk? Im prectically doing the same thing as you, just a few steps behind you! haha

How much experience do you have with all this? Because you have been getting some awesome luck on all your grows ^.^  :takingnotes:  :cheers:




I've... sort of spawned to bulk. In that Idiot Tub 2.0 exists which is just a 3.3L plastic container with some coir/verm and some leftover grain I had from grain transfers (I didn't have enough new jars sterilized to use it all.) So we'll see how that goes. I've been putting off  starting grain masters from spores (although it worked the first time), but I really probably should, the liquid culture is going to take a while to get to the point where I can use it.

Another grain jar is just about finished colonizing and despite the temptation to use it for a tiny tub of some kind I should probably just make more jars from it.

This is my first grow.


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OfflineNewbie McShroomie
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Registered: 02/14/17
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Re: A Grow Log, An Idiot Tub, Some Nerd Crap... And A Question [Re: Shameful Idiot]
    #24112313 - 02/22/17 11:08 PM (6 years, 11 months ago)

idiot tub 2.0 xD i can dig it! What tek did you use for your LC? Iv heard conflicting things about doing LC if youre a beginner, because "you wont be able to tell if your LC is contaminated until you have inoculated with it"... but hey, if it's working for an Idiot, it should work for a Newb, Right?? xD


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OfflineShameful Idiot
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Re: A Grow Log, An Idiot Tub, Some Nerd Crap... And A Question [Re: Newbie McShroomie]
    #24112387 - 02/22/17 11:53 PM (6 years, 11 months ago)

Quote:

Newbie McShroomie said:
idiot tub 2.0 xD i can dig it! What tek did you use for your LC? Iv heard conflicting things about doing LC if youre a beginner, because "you wont be able to tell if your LC is contaminated until you have inoculated with it"... but hey, if it's working for an Idiot, it should work for a Newb, Right?? xD




What's the worst that can happen, I knock up a few jars with it and they contaminate and die? That happens sometimes anyways. I mean bear in mind once you've bought a few dozen jars and are making your own spore prints, screwing around a bit doesn't actually cost money.


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OfflineNewbie McShroomie
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Re: A Grow Log, An Idiot Tub, Some Nerd Crap... And A Question [Re: Shameful Idiot]
    #24112450 - 02/23/17 12:44 AM (6 years, 11 months ago)

Man, I like the way you think. :bow2:


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OfflineShameful Idiot
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Re: A Grow Log, An Idiot Tub, Some Nerd Crap... And A Question [Re: Newbie McShroomie]
    #24113732 - 02/23/17 03:02 PM (6 years, 11 months ago)

Oh, sorry, as for what tek I used, just look for whatever basic karo tek you can find. 3-4g of karo (light corn syrup if you aren't in North America) per 100mL of water. PC'd for half an hour at 15 psi, single inoculation hole covered with micropore tape and then covered with another piece immediately after inoculation.

They were inoculated with needle biopsies of two fresh fruits. I drew maybe 2mL of distilled water into the syringe, PC'd it for 15 minutes, let it cool, sterilized the entry and exit points on the mushroom stem with alcohol (which bruises it like a MOTHERFUCKER), and slid the needle through while rotating it gently. After that, just used the water in the syringe to shoot the chunks of mushroom tissue into the karo water. Then just waited and they started growing little clouds of mycelium around them. A third attempt didn't grow anything but also didn't contaminate.

Resist the urge to flame-sterilize the needle before it goes in the karo jar. I know every cell in your body is telling you that you need to, but there's live tissue in there :smile:

edit: I know this is the simplest thing ever but I was actually confused about it initially - which water to use. I use mineral water (not distilled) to make BRF jars and LC jars, tap water for most everything else (dunking, soaking perlite, misting, grain prep, etc), and distilled water for spore syringes. I'm not sure if it would have mattered for the syringes but distilled was the jug that was closest to me. My tap water here is pretty damn good though, healthy mineral content but not hard water, naturally pretty pure, low levels of chlorine and chloramine.


Edited by Shameful Idiot (02/23/17 04:06 PM)


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OfflineNewbie McShroomie
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Re: A Grow Log, An Idiot Tub, Some Nerd Crap... And A Question [Re: Shameful Idiot]
    #24114993 - 02/24/17 12:05 AM (6 years, 10 months ago)

wozah! Thanks man! So would you suggest doing a 1/2 pint of LC? or doing agar?
Can I do LC with Spore syringe?
Liquid cultures are the one thing I have yet to really research or look into, so I dont really know much about it haha, but what you typed up with get my far! (once i have living tissue)


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OfflineShameful Idiot
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Re: A Grow Log, An Idiot Tub, Some Nerd Crap... And A Question [Re: Newbie McShroomie]
    #24115128 - 02/24/17 01:49 AM (6 years, 10 months ago)

Quote:

Newbie McShroomie said:
wozah! Thanks man! So would you suggest doing a 1/2 pint of LC? or doing agar?
Can I do LC with Spore syringe?
Liquid cultures are the one thing I have yet to really research or look into, so I dont really know much about it haha, but what you typed up with get my far! (once i have living tissue)




I'm not saying this is the right way to do it but this how I did it. I tared my scale with a tablespoon measure and then poured karo onto it until it hit 4 grams. Then I filled my 1/2-pint jars roughly 1/2 full (so 120-ish mL.) Then the PC obviously.

You can totally do it with spores too, just don't shoot a hot sticky load into it, because too many genotypes can cause harmful competition. More spores does not always equal more growth, and dark spore syringes does not equal quality spores. Every syringe I've purchased has looked perfectly clear, but here I am with mushrooms.


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OfflineShameful Idiot
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Re: A Grow Log, An Idiot Tub, Some Nerd Crap... And A Question [Re: Shameful Idiot]
    #24115155 - 02/24/17 02:21 AM (6 years, 10 months ago)

Six jars knocked up with Allen Strain from a multispore syringe,



Here's what the barcode scanner has to say about that.



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OfflineShameful Idiot
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Re: A Grow Log, An Idiot Tub, Some Nerd Crap... And A Question [Re: Shameful Idiot]
    #24120209 - 02/26/17 01:28 AM (6 years, 10 months ago)

Doing an LC of a nice toadstool I got today - hopefully it works out!

And let this be a lesson in how fast things can go wrong. I checked my fruiting chambers when I got up, probably 1:30 PM maybe? Nothing obviously amiss. I went to check again just after midnight, just now, and one cake had TWO SOLID INCHES of cobweb on it. It'd spread 3" across the perlite onto 3 other cakes and halfway up the side. In... ten and a half hours. I mean I lost an FC but I'm sort of impressed.

Obviously I took it well outside of the closet to dispose of it and I've managed to contain cobweb pretty well in there in the past, but DAMN.


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OfflineShameful Idiot
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Re: A Grow Log, An Idiot Tub, Some Nerd Crap... And A Question [Re: Shameful Idiot] * 1
    #24121722 - 02/26/17 05:26 PM (6 years, 10 months ago)

Just like my grandpa always used to say, you lose one chamber to contaminants, giant-ass mushrooms grow in the other. Well, maybe he said something like that. Probably not I guess. The Albino A+ I birthed a week ago have been going downright nuts, also resulting in what I'm pretty sure is the biggest single mushroom I've grown so far.



I'll probably do an LC of it if it's still viable by time I summon the effort to do up a couple karo jars. I've gotten perfectly healthy samples after 12 hours in the fridge before, although this one will probably only be in there for a couple hours.

In other news the grain jar I was considering convincing myself was fully colonized on Friday is now ACTUALLY good to go, so I'll probably do some grain transfers tonight. And probably another idiot tub if I have any left over because I think the other one is... some kind of fucked. These two grain masters (the one that was probably bad, and this one, which is certainly good) were a bit weird because I hadn't really done my reading and I shot the spores right down the center, not on the sides. So I thought they just hadn't colonized, and put them on a shelf, by time I saw any white at all they were nearly fully colonized and had a huge hard mass of mycelium in the middle that was near-impossible to bust up. The second jar came apart with a gentle shake and has quickly colonized the rest of the grains, so I have high hopes for that one, and five sterilized WBS jars ready to transfer it to.

I know there's some debate over WBS prep but what works for me is just rinsing it, dumping floaties, soaking it for 24 hours, then turn the stove on until it's steaming and just getting a few bubbles coming up through it, but isn't properly boiling yet, then dumping it out. Drained for 30-60 minutes in colanders over the sink or a bowl, shaking them every 10 minutes, then into the jars and into the PC for 90 mins. Nothing's looked too dry but I haven't seen water on the bottoms either. I'm a scrub so I just use polyfill stuffed through a hole.


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Offlinelovelaughlibs
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Re: A Grow Log, An Idiot Tub, Some Nerd Crap... And A Question [Re: Shameful Idiot]
    #24122021 - 02/26/17 07:22 PM (6 years, 10 months ago)

I think if you can grow a mushroom like that you should change your name ;-)


--------------------
Ask and ye shall receive; Seek and ye shall find.


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OfflineShameful Idiot
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Re: A Grow Log, An Idiot Tub, Some Nerd Crap... And A Question [Re: lovelaughlibs]
    #24122075 - 02/26/17 07:45 PM (6 years, 10 months ago)

Quote:

lovelaughlibs said:
I think if you can grow a mushroom like that you should change your name ;-)




Everyone should learn to grow mushrooms that big, it's the one time in your entire relationship your girlfriend might say "that's the biggest I've ever seen" and not be lying.

EDIT: Also did you shroom guys never take props class in theatre school? If something's gonna crack when you drill it, put gaff tape (or duct tape) on both sides of the drill site to hold it together. Seriously. Also that way, if you're using a spade bit, when it finally starts to dig into your tub, it'll still be a bit sticky from the tape and not be going as fast or hitting it as hard.

EDIT2: to be clear, drill a 1/4" pilot hole (for the center of your spade bit) using your best drilling technique, slap tape on both sides of that hole, and then go at it with your spade bit. Very carefully. There's nothing wrong with taking a while.


Edited by Shameful Idiot (02/26/17 09:19 PM)


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OfflineNewbie McShroomie
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Re: A Grow Log, An Idiot Tub, Some Nerd Crap... And A Question [Re: Shameful Idiot]
    #24128835 - 03/01/17 02:31 PM (6 years, 10 months ago)

Quote:

Shameful Idiot said:
Quote:

lovelaughlibs said:
I think if you can grow a mushroom like that you should change your name ;-)




Everyone should learn to grow mushrooms that big, it's the one time in your entire relationship your girlfriend might say "that's the biggest I've ever seen" and not be lying.

EDIT: Also did you shroom guys never take props class in theatre school? If something's gonna crack when you drill it, put gaff tape (or duct tape) on both sides of the drill site to hold it together. Seriously. Also that way, if you're using a spade bit, when it finally starts to dig into your tub, it'll still be a bit sticky from the tape and not be going as fast or hitting it as hard.

EDIT2: to be clear, drill a 1/4" pilot hole (for the center of your spade bit) using your best drilling technique, slap tape on both sides of that hole, and then go at it with your spade bit. Very carefully. There's nothing wrong with taking a while.




Or just put tape over the drill spot and drill through the tape... or is that what you mean?


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OfflineShameful Idiot
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Re: A Grow Log, An Idiot Tub, Some Nerd Crap... And A Question [Re: Newbie McShroomie]
    #24132243 - 03/02/17 08:38 PM (6 years, 10 months ago)

Quote:

Newbie McShroomie said:
Or just put tape over the drill spot and drill through the tape... or is that what you mean?




Yeah that's what I mean. Even better if you put tape on both sides of the drill spot.

Been a busy few days - out of town for a conference. Grain transfers are taking off though, I didn't leave quite enough room at the top but I still managed to distribute the colonized grain relatively well and they seem to be taking off well! On Saturday, straw shopping.



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OfflineShameful Idiot
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Re: A Grow Log, An Idiot Tub, Some Nerd Crap... And A Question [Re: Shameful Idiot]
    #24134608 - 03/03/17 09:15 PM (6 years, 10 months ago)

So I had to go out of town for a couple days, I picked everything that wasn't a tiny pin, and I come back to this.



I'll probably print the albino one there, the huge one looks to have dropped quite a lot of spores already. The grain jars also really took off, all 6 of them, so once those are fully colonized, I'll use one to make more jars, and five to make my first actual tub.


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OfflineShameful Idiot
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Re: A Grow Log, An Idiot Tub, Some Nerd Crap... And A Question [Re: Shameful Idiot]
    #24137499 - 03/04/17 11:38 PM (6 years, 10 months ago)

"And here's what it looks like when something goes wrong."

I've lost my share of jars and fruiting cakes to contaminants, but this is extra-special. I was rearranging some books on my contaminant wall of shame and this one has apparently been hiding behind a book for a couple months. So here's what a couple months of lipstick fungus looks like.



Next stop: the trash, still in the jar.


Edited by Shameful Idiot (03/04/17 11:44 PM)


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OfflineWalkingbio
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Re: A Grow Log, An Idiot Tub, Some Nerd Crap... And A Question [Re: Shameful Idiot]
    #24137596 - 03/05/17 01:11 AM (6 years, 10 months ago)

I have read every post in this thread, this is hilarious and very educational. Thank you for posting this, I'll definitely keep up to date on this thread keep pumping them out man!


--------------------
"It's the moment you face your fears, you realize you have the strength and desire to keep fighting." - Walkingbio


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OfflineShameful Idiot
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Re: A Grow Log, An Idiot Tub, Some Nerd Crap... And A Question [Re: Walkingbio]
    #24137612 - 03/05/17 01:25 AM (6 years, 10 months ago)

Quote:

Walkingbio said:
I have read every post in this thread, this is hilarious and very educational. Thank you for posting this, I'll definitely keep up to date on this thread keep pumping them out man!




You want hilarious? I'll give you hilarious.

So a few weeks ago I had a horrible cobweb problem in a fruiting chamber. I mentioned it earlier, over the course of 10 hours it went from being totally fine to there being like an inch of cobweb covering one entire cake and touching three others. I bagged up all the cakes and wrote it off to not paying close enough attention.

Tonight, I'm dealing with a tiny cobweb problem in another FC. Just the tiniest little puff of it, nothing at all. I hit it with the peroxide, and tell my girlfriend that it turned black instantly and sizzled and was super cool, she wanted to see it. So I realize that while I bagged up those cakes, I never threw them out, so I can probably just go take them somewhere away from everything else and get a sweet cobweb snuff film going with the peroxide spray. So I rip open the bag, which was sitting in the corner right where I left it.

So yeah, that one cake still had cobweb. Sort of. It'd killed half of it. That cake is still a write-off but it put up a good fight. The other five cakes are absolutely perfect, despite being sealed in a plastic bag for two weeks with nine trillion cobweb spores. Not only are they fine, I picked fucking fifty wet grams off them, including this seven inch long behemoth that was actually making the bag tent up.



So now I have five perfectly good cakes to find a home for. I still don't entirely trust them to not contaminate something else, but I have an empty FC they can all live in, I guess we'll see what happens.

Moral of the story: never give up on these weird fuckers.


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OfflineWalkingbio
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Re: A Grow Log, An Idiot Tub, Some Nerd Crap... And A Question [Re: Shameful Idiot]
    #24138149 - 03/05/17 09:12 AM (6 years, 10 months ago)

Fucking hilarious lol I haven't even started my first grow yet I'm nervous about getting a contamination the central air filters are DISGUSTING in my house. I don't have the money to replace them right now so I figured I'll do the inoculation in a still air box that has been sterilized. I'm going to cover the vent in my room with a wet towel and then spray down the bedroom and closest focusing more on the closet, then hopefully get a clean spawn bag out of it. Still scared, I won't be using jars just one big bag of grain good chance to mess up everything for me./:


--------------------
"It's the moment you face your fears, you realize you have the strength and desire to keep fighting." - Walkingbio


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Offlinelovelaughlibs
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Re: A Grow Log, An Idiot Tub, Some Nerd Crap... And A Question [Re: Walkingbio]
    #24138884 - 03/05/17 02:24 PM (6 years, 10 months ago)

Quote:

Walkingbio said:
Fucking hilarious lol I haven't even started my first grow yet I'm nervous about getting a contamination the central air filters are DISGUSTING in my house. I don't have the money to replace them right now so I figured I'll do the inoculation in a still air box that has been sterilized. I'm going to cover the vent in my room with a wet towel and then spray down the bedroom and closest focusing more on the closet, then hopefully get a clean spawn bag out of it. Still scared, I won't be using jars just one big bag of grain good chance to mess up everything for me./:



Don't worry too much, the air is utterly filthy almost everywhere on the planet. It comes down to technique - you don't need to sterilise the SAB, just spray the walls with some lightly soaped water and work at a reasonably quick speed without stirring up air currents.

You know to sterilise bags for a lot longer than jars also, right? The heat takes longer to penetrate all the way to the middle of the grains. I've heard 3 hours is a good time but I'm not an expert.


--------------------
Ask and ye shall receive; Seek and ye shall find.


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