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OfflineGet Shwifty
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Registered: 10/14/15
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The bad, the ugly, and the uglier
    #24005892 - 01/12/17 11:17 PM (7 years, 18 days ago)

Alright, so I inoculated these WBS jars a week ago with what appeared to be clean agar that was shaken in a sterilized jar of water and then added to each jar inside an SAB.

My friend thinks some of these could just be trying to fruit since the temperatures got a tad cold in the closet this week. I think it is bacteria. I won't be using gypsum anymore with my WBS because it makes the jars look so dusty/dirty when I shake them.

I will let you be the judge. Thank you for looking at these, I really appreciate your input guys.



- Mexican Dutch King

- Mexican Dutch King

- Mestizo

- Mestizo


- Mestizo

- Mestizo


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Offlineenlightenment
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Re: The bad, the ugly, and the uglier [Re: Get Shwifty]
    #24006609 - 01/13/17 09:13 AM (7 years, 17 days ago)

every jar looks contaminated :/
How did you prepare the WBS? Inoculated with a spore syringe?
Try to use one a few drops per jar. There are plenty spores in every drop of spore solution.


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OfflineGet Shwifty
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Registered: 10/14/15
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Last seen: 8 months, 18 days
Re: The bad, the ugly, and the uglier [Re: enlightenment]
    #24006949 - 01/13/17 11:48 AM (7 years, 17 days ago)

The WBS was prepared using this tek.

I dropped the colonized agar into 200ml of pressure cooked distilled water in a mason jar and then shook it prior to inoculation of these WBS jars. I did not use a syringe.

I probably hovered my hand over the jar too long in the SAB, or maybe my agar was just bacterial from the start and I couldn't see it with my inexperienced color blind eyes.

Thanks friend


Edited by Get Shwifty (01/13/17 11:53 AM)


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