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OfflineLiminal_God
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Registered: 06/05/14
Posts: 60
Last seen: 4 years, 22 days
(Pics) Morel Clones on Agar
    #24005259 - 01/12/17 07:15 PM (7 years, 18 days ago)

Hey y'all, I just wanted to share pics of my first attempt at cloning. I started with dry fruits bought online.  Unfortunately, the species was not identified with what I bought; my guess is Morchella angusticeps (They came from Idaho). Samples were hydrated in distilled water overnight, they were then given 5 washes in, sterilized, distilled water.  Fruit tissue was dropped on the plates on 1/8. This first pic is two days later on the 10th with the first signs of growth.



These each are a pic of the 5 dishes I started, taken today (1/12), after making my first transfers.







So far growth definitely seems strong and I'm excited to grow them out further.  Like I said this is my first ever attempt at cloning in general and I'm definitely still a beginner when it comes to agar work. If anyone has any advise, please share! Also, I should add that I do realize the possibility that these aren't clones. Definitely a chance that the growth is from spores still attached to the fruit. Regardless, I'm happy I even have the growth that I do.


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Offlineprojectbadass
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Registered: 12/20/16
Posts: 100
Loc: Northeast Ohio Flag
Last seen: 2 years, 7 months
Re: (Pics) Morel Clones on Agar [Re: Liminal_God]
    #24005280 - 01/12/17 07:22 PM (7 years, 18 days ago)

Badass!


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OfflinePinPornProducer
Buy the ticket, take the ride
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Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I Flag
Last seen: 6 years, 24 days
Re: (Pics) Morel Clones on Agar [Re: Liminal_God]
    #24005338 - 01/12/17 07:41 PM (7 years, 18 days ago)

How do you plan on getting fruits?


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OfflineLiminal_God
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Registered: 06/05/14
Posts: 60
Last seen: 4 years, 22 days
Re: (Pics) Morel Clones on Agar [Re: PinPornProducer]
    #24005464 - 01/12/17 08:21 PM (7 years, 18 days ago)

Thanks projectbadass!  :thumbup:
PinPornProducer I'll face that hurdle when I get to it. Right now my focus getting a clean, healthy culture expanded to spawn, then sclerotia growth. But, when I do my plan is to try to replicate current successes as best as possible and then experiment from there.


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OfflineLiminal_God
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Registered: 06/05/14
Posts: 60
Last seen: 4 years, 22 days
Re: (Pics) Morel Clones on Agar [Re: Liminal_God]
    #24009445 - 01/14/17 10:34 AM (7 years, 16 days ago)

Update: Just checked and all my transfer dishes are doing good and currently free of contamination. The the mycelium is currently expanding ~1/4" a day. The starter dishes are all 100% colonized and some appear to be starting to form micro-sclerotia.  Also, one of two petris I started with Esculenta spawn, I thought was bad, is showing clean growth.
I'll try to have some updated pictures later today or tomorrow.


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OfflineLiminal_God
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Registered: 06/05/14
Posts: 60
Last seen: 4 years, 22 days
Re: (Pics) Morel Clones on Agar [Re: Liminal_God]
    #24012246 - 01/15/17 01:32 PM (7 years, 15 days ago)

Here are two of the 6 transfers I did. Big ramp up in growth since yesterday.




Here is the a pic of the petri from Esculenta spawn nearing full colonization.



And finally a pic of one of the mother petris. I mentioned yesterday how they were all fully colonized. Now they are all starting to get more fluffy growth. This one even expanding all the way up the side and onto the lid.



Enjoy!


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OfflineLiminal_God
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Registered: 06/05/14
Posts: 60
Last seen: 4 years, 22 days
Re: (Pics) Morel Clones on Agar [Re: Liminal_God]
    #24028908 - 01/21/17 06:44 PM (7 years, 9 days ago)

Here's a pic of one of the fully colonized transfer dishes.  I just transferred a piece of each of the 7 petris to pint jars filled with various spawn media I'll be testing.  I'll probably be starting up a grow log for those as they progress.



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OfflineRolledUhhp
Amateur Cultivator

Registered: 08/10/16
Posts: 246
Last seen: 3 years, 6 months
Re: (Pics) Morel Clones on Agar [Re: Liminal_God]
    #24030383 - 01/22/17 12:18 PM (7 years, 8 days ago)

Awesome, man! Thanks for sharing.


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OfflineHaywire
Wetspot Wizard
Male


Registered: 12/29/13
Posts: 1,611
Last seen: 5 days, 20 hours
Re: (Pics) Morel Clones on Agar [Re: RolledUhhp]
    #24032299 - 01/23/17 12:38 AM (7 years, 7 days ago)

Take a look at this, if you haven't already.

Haven't had any succes yet, but I keep at it.


--------------------
Ciao mamma, guarda come mi diverto


My grows :mushroom2: Outdoor patches


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OfflineLiminal_God
Stranger

Registered: 06/05/14
Posts: 60
Last seen: 4 years, 22 days
Re: (Pics) Morel Clones on Agar [Re: Haywire]
    #24034818 - 01/23/17 11:30 PM (7 years, 6 days ago)

Quote:

Haywire said:
Take a look at this, if you haven't already.

Haven't had any succes yet, but I keep at it.




Thanks, I have.  Probably enough times to recite it by heart haha. Yeah, it's only a matter of time! Next thing we know morel will be the new oyster.


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OfflineHaywire
Wetspot Wizard
Male


Registered: 12/29/13
Posts: 1,611
Last seen: 5 days, 20 hours
Re: (Pics) Morel Clones on Agar [Re: Liminal_God]
    #24034894 - 01/24/17 12:19 AM (7 years, 6 days ago)

Quote:

Liminal_God said:
Thanks, I have.  Probably enough times to recite it by heart haha. Yeah, it's only a matter of time! Next thing we know morel will be the new oyster.




You wish :wink:


--------------------
Ciao mamma, guarda come mi diverto


My grows :mushroom2: Outdoor patches


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OfflineLiminal_God
Stranger

Registered: 06/05/14
Posts: 60
Last seen: 4 years, 22 days
Re: (Pics) Morel Clones on Agar [Re: Haywire]
    #24199581 - 03/28/17 11:54 AM (6 years, 9 months ago)

For anyone interested, I finally started a grow log here:
https://www.shroomery.org/forums/showflat.php/Number/24199548

Not a whole lot up yet, but over the next couple weeks I'll definitely be adding more. Just wanted to get it started otherwise I woulda never started it haha


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