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Corona11
The New Guy



Registered: 12/09/16
Posts: 151
Last seen: 6 years, 2 days
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Re: First Agar project. AA+ [Re: Corona11]
#24045586 - 01/28/17 01:25 AM (7 years, 3 days ago) |
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Well I ran into a really bad contam problem. Lets see if I can save this one. The other ones I tossed because they were worse off than this one.
Took six samples of this one. As always prepared my work space and stage my gear.

Use 70% iso alcohol
Edited by Corona11 (01/28/17 01:26 AM)
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Corona11
The New Guy



Registered: 12/09/16
Posts: 151
Last seen: 6 years, 2 days
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Re: First Agar project. AA+ [Re: Corona11]
#24045588 - 01/28/17 01:28 AM (7 years, 3 days ago) |
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After some comments made in LAGM2017
Quote:
ComebackKid said: Wheres the contamination coming from? If it's coming from your transfered you just have more cleaning to do. If they're at the edges of your plates than you need to tighten up sterile tech
With this advice I have started paying close attention to getting under the lip. What I don't have pictures capturing is me wiping the jars and lips again before starting the transfer process in the SAB.

So everything's sanitized, and staged inside the SAB. I being to initiate transfer, but first a pic of the contamed culture.
03 AA+ 03 02< 03 AA+ 03 01-06

The new dishes are staged and wiped again, the knife flamed to red hot.

Same tek as before, keeping hands clear of the air above the sample being taken.

After six transfers, wiping each jar before getting ready to tranfers, then flame sterilizing the blade and completing transfer.

I know there is a lot of repeated information but it's important to remeber all the steps, there are a lot!! So sterilization, and accurate blade work.
I know that along the bottom there were no contams and I didnt transfer. Its because I have read to keep away from the edges and the mycelium i transfered has started to become stringy and I'm not sure if sectoring, or these are the "strains" that come before sectoring.
From these transfers by my count this makes number 4. I plan to grow a few healthy plates and spawn on to grain then to multiple jars via G2G, and then fruit.
Sorry if this is taking so long and you all are losing interest. But its been a great way for me to track my progress in learning for Agar cultivation. This is very tedious and also rewarding. Some of the cultures I've had have been beautiful already. Like this KSSS I have going. 2nd transfer, I made 10 transfers of it tonight.

Thanks for all the help and support guys. As I stated in the beginning the purpose of this is a learning experience, and for me to keep track of my own persoal growth. Also some newbies like myself could learn a great deal just by my mistakes and your pointing them out. All my success is owed to yall for the corrective criticism.
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
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Re: First Agar project. AA+ [Re: Corona11]
#24045702 - 01/28/17 04:54 AM (7 years, 2 days ago) |
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Good work! Dont know what to say about yoy being a leftie though lol
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: First Agar project. AA+ [Re: Kenetic]
#24045823 - 01/28/17 07:58 AM (7 years, 2 days ago) |
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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Re: First Agar project. AA+ [Re: Corona11]
#24045860 - 01/28/17 08:23 AM (7 years, 2 days ago) |
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Hey Corona, you might try some work without the gloves, tape and sleeves and see how it comes out. That is way overkill IMO not to mention a pain in the ass to put on every time you work with agar. I never wear gloves and rarely get contams. Give it a try...might save you some hassle. Nice work, nonetheless!
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Corona11
The New Guy



Registered: 12/09/16
Posts: 151
Last seen: 6 years, 2 days
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Re: First Agar project. AA+ [Re: Mycolorado]
#24047177 - 01/28/17 06:28 PM (7 years, 2 days ago) |
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Thanks for the support and advice guys,
@Mycolorado: I did a G2A transfer today with just my hands. Well see.. Got a fresh new pair of tweezers and put them with my gear. If theres no contams il be sold. Its out of the realms of the log but thought id try it. Didnt change anything in my tek except i didnt put a fresh shirt on and gloves.
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
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Re: First Agar project. AA+ [Re: Corona11]
#24048090 - 01/29/17 06:16 AM (7 years, 1 day ago) |
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Sometimes I wear gloves sometimes I dont. I prefer gloves but the rest of the stuff you had going on is a little outdated. Ive never had "dead skin cells from my arms" cause any problems, i wipe them with iso anway.
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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Corona11
The New Guy



Registered: 12/09/16
Posts: 151
Last seen: 6 years, 2 days
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Re: First Agar project. AA+ [Re: Kenetic]
#24048317 - 01/29/17 08:43 AM (7 years, 1 day ago) |
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Nice advice kenetic. Im going to try this, I didnt think it was outdated info, seemed like common sense lol. Thats what I did yesterday wiped myself with iso and began working. I have some petris in the mail, next weekend ill be using those for transfers. I dont really like this prepour mini round method as well as I did in begining. I just would prefer to have petris where i can see in clearly and not have to look through the bottom or the sides. Colored lids make it a little harder to see through.
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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Re: First Agar project. AA+ [Re: Corona11]
#24048365 - 01/29/17 09:08 AM (7 years, 1 day ago) |
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Quote:
Corona11 said: Nice advice kenetic. Im going to try this, I didnt think it was outdated info, seemed like common sense lol. Thats what I did yesterday wiped myself with iso and began working. I have some petris in the mail, next weekend ill be using those for transfers. I dont really like this prepour mini round method as well as I did in begining. I just would prefer to have petris where i can see in clearly and not have to look through the bottom or the sides. Colored lids make it a little harder to see through. 
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mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 4 months
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Re: First Agar project. AA+ [Re: Mycolorado]
#24048374 - 01/29/17 09:12 AM (7 years, 1 day ago) |
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Subscribing love AA+ projects. Good luck mate
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
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Corona11
The New Guy



Registered: 12/09/16
Posts: 151
Last seen: 6 years, 2 days
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Re: First Agar project. AA+ [Re: mrmazdarx9]
#24049648 - 01/29/17 05:21 PM (7 years, 1 day ago) |
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Ok guys need some advice. You know I do my reading and everything. Or at least try to read as much as I can so ill try not to ask a stupid newbie question here. But..
With the transfers I just did, assuming they turn out fine. I would like to spawn to grain or pf jars and fruit to see how the mycelium will fruit.
Heres my toss up.. Do I get millet, or use rye, to do wedge transfers, or do I do a LQ and use that to knock up like a million PF jars?
Just some thoughts ive had. There are pros and cons to both the way I see it.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: First Agar project. AA+ [Re: Corona11]
#24049826 - 01/29/17 06:32 PM (7 years, 1 day ago) |
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grains all the way.
do not spawn on a large scale with cakes. it works.. so does jerkin it.. but id prefer the real thing.
also grains give you more points of inoc since the are individual pieces.
who wants to make a million pf jars. just do quarts of millet 24 hr soak. no simmering. pc 2hrs. rock out with your rock out.
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Corona11
The New Guy



Registered: 12/09/16
Posts: 151
Last seen: 6 years, 2 days
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Re: First Agar project. AA+ [Re: mushboy]
#24050102 - 01/29/17 08:03 PM (7 years, 1 day ago) |
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Ok cool thanks mushboy. I don't wana jynx myself. Got another week or so before they will even be ready. But like always gathering information incase everything goes right. Cant wait to get the petri dishes and what not. I ordered 1 section and 3 section petris. A flask and breathable corks parafilm and Pda powder mix. So when I do my next transfer Ill also be updating my tek. Im really hoping to fruit one of these really soon, feel like its taking forever to get a good culture. I guess this is where the patience comes into play huh.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: First Agar project. AA+ [Re: Corona11]
#24050555 - 01/29/17 11:39 PM (7 years, 1 day ago) |
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dont try to get a good culture going on agar.. it will take forever. if you do that just keep dumping 'undesirable' plates in quart jars and fruit them 1quart at a time. hunt for clones while you wait for a desirable culture to grow.
also dont judge a culture solely on how it looks on agar. ive had sllooooow ass puffy cultures rip through a quart jar in no time and put out decent fruits. you will never know until you fruit it anyway. wouldnt it suck to finally have a purddy agar culture only to find out its non fruiting/bunk..(rare but it happens)
i also just ordered petris. im graduating past plates. but man o man do i respect the plate
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noland
Renegade


Registered: 12/08/16
Posts: 66
Last seen: 6 years, 7 months
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Re: First Agar project. AA+ [Re: mushboy]
#24050602 - 01/30/17 12:10 AM (7 years, 1 day ago) |
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Quote:
mushboy said: dont try to get a good culture going on agar.. it will take forever. if you do that just keep dumping 'undesirable' plates in quart jars and fruit them 1quart at a time. hunt for clones while you wait for a desirable culture to grow.
also dont judge a culture solely on how it looks on agar. ive had sllooooow ass puffy cultures rip through a quart jar in no time and put out decent fruits. you will never know until you fruit it anyway. wouldnt it suck to finally have a purddy agar culture only to find out its non fruiting/bunk..(rare but it happens)
i also just ordered petris. im graduating past plates. but man o man do i respect the plate
Man I was literally just getting ready to make a thread asking about this. I was curious how everyone got their "perfect" strain isolation. Is it really best to transfer out multiple isolates and then fruit them all individually to see how they do? Or would it be more practical just to get a clean culture, which isn't an isolate, fruit it, and then clone your fruit of choice?
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: First Agar project. AA+ [Re: noland]
#24050608 - 01/30/17 12:14 AM (7 years, 1 day ago) |
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at first... just go for a clean culture and grow out fruits and find clones. its way faster then doing a billion transfers and gives you a reasonable amount of consistency while you do your thing on agar.
i started agar back in Aug. im STILL transfers from that first plate a grew to see what happens. months later it still looks like the same freakin culture. im probably missing a step but my point is still relevant.
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noland
Renegade


Registered: 12/08/16
Posts: 66
Last seen: 6 years, 7 months
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Re: First Agar project. AA+ [Re: mushboy]
#24050633 - 01/30/17 12:29 AM (7 years, 1 day ago) |
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Yeah I was thinking the same thing. I just put a couple different MS prints to agar and thought it'd be cool to transfer them out until I got a couple monocultures to work with. But then it had me thinking that this was gonna take a while, and I was just curious if that's what everyone did. Finding a good clone does seem a lot faster.
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Corona11
The New Guy



Registered: 12/09/16
Posts: 151
Last seen: 6 years, 2 days
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Re: First Agar project. AA+ [Re: noland]
#24050827 - 01/30/17 06:30 AM (7 years, 19 hours ago) |
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Hey noland we basically registered on the sme day lol.
Thanks mush. So ill go ahead and try to fruit these when they are ready and then take a clone and start another iso.
Wow so this will be a long long long thread.. But i guess once i have an awesome sample I can send it out to all you who have been following along. Well like I said this is just a learning exp.
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noland
Renegade


Registered: 12/08/16
Posts: 66
Last seen: 6 years, 7 months
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Re: First Agar project. AA+ [Re: Corona11]
#24051626 - 01/30/17 12:34 PM (7 years, 13 hours ago) |
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Quote:
Corona11 said: Hey noland we basically registered on the sme day lol.
Haha didn't even see that. We're pretty much doing the same thing too as I just put a MS syringe of AA+ to agar as well. I haven't transferred yet, so your plates look a lot cleaner than mine. Can't decide where I should transfer from... 
Good luck to you though! I'll be following along.
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Corona11
The New Guy



Registered: 12/09/16
Posts: 151
Last seen: 6 years, 2 days
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Re: First Agar project. AA+ [Re: noland]
#24051740 - 01/30/17 01:17 PM (7 years, 13 hours ago) |
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Lol thanks but mine are not clean, last transfer had a crap ton of pin mold i think. Ill be on the lookout. I have a lot of info bookmarked so if you need a tek or something just message me. Id love to help out if I can.
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