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Corona11
The New Guy



Registered: 12/09/16
Posts: 151
Last seen: 6 years, 2 days
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First Agar project. AA+ 1
#24003448 - 01/12/17 09:05 AM (7 years, 18 days ago) |
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Hello all, This is my first journal/ grow log so bear with me please.
So I am brand new to this and like Kenetic said , “if you fail try again, and again if you must”. Well I must, plus I figured if I succeed I have a good Isolation of an AA+, if not, I have learned what to do and what not to do and improved some my technique and knowledge along the way. Please give me advice and corrective criticism where it is needed. Next time I do a transfer I will upload pictures of my SAB and my agar process. My motivation behind this is the thread. Lets grow mushrooms 2017. And some beautiful pictures of isolations.
NOTE: Again I AM open to advice and corrective criticism, please be respectful, but I also do like to joke hah.
Everything is done by a few different TEK’s. The agar recipes are taken from the general cultivation part General Cultivation section, subsection agar. I first mix my ingredients dry, then add to cold water (I add extra water for the boiling portion, not much more though) and stir. Once the mixture is well dissolved, I pour it into a pot and bring to a boil for a few minutes. After it has boiled for a few minute I then move the sauce pan off the burner and move to the kitchen sink. I run the Agar through a painter’s net strainer, to remove any bubble and film, it also has produced very clear agar for me.
UPDATE!!!!!
Quote:
bodhisatta said: I've never pressure cooker agar more than a half hour. It only takes 15-20 minutes.
Wrap the edges of your dishes not the whole dish.
Find my agar TEK through the links in my signature
Nice job 
After the agar has been strained it is put into a glass measuringjar for pouring, I pre-pour using Pastywhyte's Tek and modified lids, PC @15 PSI for 20 min, as the PC was cooling I began to prepare my area for inoculation/transfer. I turn all fans, heat, or AC off, ensure all windows are closed and keep the pets free and clear of the area. I spray the area down with bleach/water mix, and then wipe my tools down with alcohol. I take a shower and put clean clothes and a hat on. When the PC is cool enough to touch, I remove agar from the PC and then place it within my SAB and all my equipment is staged in this area.
Along with wiping down the surfaces and tools and equipment I have started to wipe the jars and petri dishes as well. This initial escaped my mind, and why as a beginner it is very important for me to read the TEKs many times, remember the process, and before I start a transfer or inoculation I now rehearse it in my head and stage my jars accordingly.
UPDATE!!!
(Note), the first parent culture was done in ½ pint mason jars in a pre-pour tek that worked very well. I just started to use the petri dish tek so I could see the cultures more clearly. What sparked this notion of mine is I saw a picture of what I was told is a true mono-culture (or close to it), and it was beautiful. So I decided I wanted one and this is where we are. I know I am “sooo new”, but I have a dream and ambition, let’s see where this takes me. I know I will fail many times, but from my readings RR says to take many different samples of the same things (assuming it is a clean and god sample). With this I will have some fail and some survive. I plan to fruit the strains after the 5th transfer and definite strands begin appearing (I forget the proper terminology filaments?). I read threads and people failures and success all day long (in my free time), along with a book that I purchased, psilocybe cultivation handbook.
So now that my TEK and background/reasoning is done I will start on the log for what I have now.
12/26 MS syringe purchased from a trusted vendor and I used a pre-pour method on these jars and it worked out very well, I just decided to better see the mycelium growth. MAE recipe (500 ml)
Jar 1 First Generation

Jar 2 First Generation

Jar Three First Generation

01/10/17 MEA Recipe (500 ml) As you can tell I am still new my transfers are BIG clumps of agar and little pieces of mycelium. I will hone in these TEK's as I progress.


Jar 2

Jar 3

These are examples of my petri dishes and the clarity I mentioned.



01/14/2017 UPDATE!!!! Jar 3. 2nd Gen Due to inexperience I took a sample from the wrong portion. (It was my thought) That where I took the sample I could see definite strands of mycelium groups, so I tried to get a piece of these strands. However, I learned that the best culture would have been from the far right of the clump, “have to crack a few eggs..” right?

Jar 2, 2nd Gen Jar two was thrown away due to contaminants, I think. The growth was abnormal and it looked a little slimy in spots so I just threw it away.

Jar 1, 2nd Gen This specimen is still in incubation because I didn’t think that it was ready and wanted to see if more definition could be grown.
I am concerned for this one because it has all these spots, but in my defense when I took the transfer (lack of skill and awareness) the sample didn’t stick to the agar. So the sample slid all around the jar as I tried and tried to tap and move it to the center when I finally gave up.

Here is one of the petri dish samples taken from one of the 1st generation jars, it is a 2nd gen and has been transplanted again. Again poor TEK I didn't choose the best specimen for my transfer. Next time more mental preparation and attention will be taken into account.

 Thanks for all the comments and support guys, I have already learned a whole bunch and much more to come. I know I need to practice on my bladesmanship hah. I am not very accurate and only time and practice will fix it.
More updates in about a week.
Edited by Corona11 (01/14/17 12:44 PM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: First Agar project. AA+ [Re: Corona11]
#24003460 - 01/12/17 09:10 AM (7 years, 18 days ago) |
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I've never pressure cooker agar more than a half hour. It only takes 15-20 minutes.
Wrap the edges of your dishes not the whole dish.
Find my agar TEK through the links in my signature
Nice job
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
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Re: First Agar project. AA+ [Re: Corona11]
#24003475 - 01/12/17 09:17 AM (7 years, 18 days ago) |
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Looking good so far! You did pour your agar pretty thick though, wont hurt but its also not necessary. I try to go about a 1/4" thick.
Also, you dont need to pc the agar for 60 minutes, 20 min at 15 psi is good enough. I do 30 at 10 psi so i can use my smaller pc.
Keep up the good work!
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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Corona11
The New Guy



Registered: 12/09/16
Posts: 151
Last seen: 6 years, 2 days
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Re: First Agar project. AA+ [Re: Kenetic]
#24003542 - 01/12/17 09:43 AM (7 years, 18 days ago) |
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Quote:
bodhisatta said: I've never pressure cooker agar more than a half hour. It only takes 15-20 minutes.
Wrap the edges of your dishes not the whole dish.
Find my agar TEK through the links in my signature
Nice job 
Thank you. I have started shifting throughyour teks. Funny thing is I have read a couple of them before. Im pretty sure it was your no pour tek that I imitated in my no pour, as well as straining it. But you have reminded me of things, I will continue reading and learning. You don't think any of these look like contams?
Quote:
kenetic said: Looking good so far! You did pour your agar pretty thick though, wont hurt but its also not necessary. I try to go about a 1/4" thick.
Also, you dont need to pc the agar for 60 minutes, 20 min at 15 psi is good enough. I do 30 at 10 psi so i can use my smaller pc.
Keep up the good work!
Thank you. I am trying to get used to everything. I find it hard to see in my SAB, I will end up making another one when payday comes. I will continue to post the progress or failure of my journey here.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: First Agar project. AA+ [Re: Corona11] 1
#24003786 - 01/12/17 11:16 AM (7 years, 18 days ago) |
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Corona11
The New Guy



Registered: 12/09/16
Posts: 151
Last seen: 6 years, 2 days
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Re: First Agar project. AA+ [Re: bodhisatta]
#24004018 - 01/12/17 12:59 PM (7 years, 18 days ago) |
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Quote:
bodhisatta said: https://www.shroomery.org/forums/showflat.php/Number/23990888
https://www.shroomery.org/forums/showflat.php/Number/21922023
Thanks bod. Someone said those look like pin mold and not myc. Do you think this true also Bod? I still am learning what good and bad looks like.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: First Agar project. AA+ [Re: Corona11]
#24004047 - 01/12/17 01:09 PM (7 years, 18 days ago) |
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Nah, jar three looks best tho
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: First Agar project. AA+ [Re: bodhisatta]
#24004487 - 01/12/17 03:24 PM (7 years, 18 days ago) |
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i in your journal.
good luck
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Corona11
The New Guy



Registered: 12/09/16
Posts: 151
Last seen: 6 years, 2 days
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Re: First Agar project. AA+ [Re: mushboy]
#24005214 - 01/12/17 07:03 PM (7 years, 18 days ago) |
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This is what i was going to put on grain tonight.... But it smelled really sweet. So... I didnt.
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
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Re: First Agar project. AA+ [Re: Corona11]
#24005235 - 01/12/17 07:08 PM (7 years, 18 days ago) |
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That? No way dude. It's not nearly colonized enough. Wait a few more days at least
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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Re: First Agar project. AA+ [Re: Corona11]
#24005236 - 01/12/17 07:08 PM (7 years, 18 days ago) |
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Clean or not I would suggest you take some transfers from strong growth and get nice clean cultures and then go to to grain.
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Corona11
The New Guy



Registered: 12/09/16
Posts: 151
Last seen: 6 years, 2 days
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Re: First Agar project. AA+ [Re: Kenetic]
#24005251 - 01/12/17 07:14 PM (7 years, 18 days ago) |
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Quote:
kenetic said: That? No way dude. It's not nearly colonized enough. Wait a few more days at least
Too soon?
Edited by Corona11 (01/12/17 07:14 PM)
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: First Agar project. AA+ [Re: Corona11] 1
#24005279 - 01/12/17 07:21 PM (7 years, 18 days ago) |
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way to soon. and you should only have one 'culture' of growth that you would use.
you need to cut a healthy piece away and grow that guy and maybe cut again to ensure its clean. then use it as you wish.
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Corona11
The New Guy



Registered: 12/09/16
Posts: 151
Last seen: 6 years, 2 days
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Re: First Agar project. AA+ [Re: mushboy]
#24009742 - 01/14/17 12:45 PM (7 years, 16 days ago) |
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Updates of pics, and some of the reading material has been edited and recognition of errors addressed. Thank you all for the support looking forward to fruiting one of these soonish.
Edited by Corona11 (01/14/17 01:28 PM)
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Fick_Duck
Truffle Shuffle



Registered: 10/18/02
Posts: 247
Loc: The Hamburger Train
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Re: First Agar project. AA+ [Re: Corona11]
#24009806 - 01/14/17 01:22 PM (7 years, 16 days ago) |
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I admire your research and research approach.
I'm signed up to watch. Good luck!
-------------------- "To know life you must fuck it in the liver." -Dr. Frankenstein, Andy Warhols Frankenstein
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: First Agar project. AA+ [Re: Fick_Duck] 1
#24009963 - 01/14/17 02:50 PM (7 years, 16 days ago) |
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Hey man just a tip, its a little easier for people to follow and help if you put the details of your updatein your last post and then update the OP if you want after
--------------------
Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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Corona11
The New Guy



Registered: 12/09/16
Posts: 151
Last seen: 6 years, 2 days
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Ok so i had a few set backs... Due to poor labeling I lost track of what samples came from what cultures. So i decided to stick with the only one I knew for sure.
Meet subject Charlie. Or as I like to call him 03-03-AA+ 3rd transfer, 3rd jar,

Since I didnt update correctly last time I figured I would throw some things in now.

I ready my area and clean my tools. I lay some paper towels in a Tupperware container and douse it with alcohol. I use these to sterilize any and all equipment before it enters the SAB.

Unfortunately my assistant didnt get pictures of me wiping any other container except this one.

Once everything is inside and sterilized I wipe my hand down again and go inside (thats what she said). I flame my blade after wiping down the hilt, and my hands.

Keeping my hands away from the area above the sample I am attempting to extract. I carefully select my sector of choice.

After each transfer I wipe the blade and flame.

After all seven transfers were done I labeled, 4th transfer jars 1-7, and placed in incubation.
A shot after all was done.

Looking forward to the next set of advice, thanks for all the help and support guys. See you again in a week or so.
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
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Re: First Agar project. AA+ [Re: Corona11]
#24029707 - 01/22/17 05:36 AM (7 years, 8 days ago) |
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Nice update! Plates look nice and clean too. Just remember to be careful with all that alcohol, which btw sanitizes, not sterilizes. Youre almost ready for expansion now, I'll be keeping track
Wish I had an assistant!
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: First Agar project. AA+ [Re: Kenetic]
#24029885 - 01/22/17 08:40 AM (7 years, 8 days ago) |
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Corona11
The New Guy



Registered: 12/09/16
Posts: 151
Last seen: 6 years, 2 days
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Re: First Agar project. AA+ [Re: Kenetic]
#24030011 - 01/22/17 09:32 AM (7 years, 8 days ago) |
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Quote:
kenetic said: Nice update! Plates look nice and clean too. Just remember to be careful with all that alcohol, which btw sanitizes, not sterilizes. Youre almost ready for expansion now, I'll be keeping track
Wish I had an assistant!
I planned on keeping one for further isolation and the rest would go to grain for fruiting. Its nice having an extra set of hands when i need them.. Most the time she just watches from afar lol.
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