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Crispykoot
Jello Wrangler



Registered: 10/16/16
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Transparent mycelium on agar
#24003297 - 01/12/17 07:36 AM (7 years, 18 days ago) |
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Hey folks..Just a quick question about a bunch of plates that I transferred, some MS, some clones. All are throwing clear myc. The temps have dropped in the incubation room from 78-80 F down to 68-72 with this cold.
My question is: Barring bacterial issues, which I could have, but lets say I don't for now...Can cold cause this? It feels like the myc is like "fuck this, warm me up... I can't do that right now."
I may post pics. Starting to fall down a bit of a rabbit hole of non understanding. Thank you.
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Mushenstein
Brain Damage



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Re: Transparent mycelium on agar [Re: Crispykoot]
#24003312 - 01/12/17 07:47 AM (7 years, 18 days ago) |
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you will get responses quicker if you post pics. Without them everyone is just guessing.
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Tiamo
Trust in LITFA




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Re: Transparent mycelium on agar [Re: Crispykoot]
#24003316 - 01/12/17 07:50 AM (7 years, 18 days ago) |
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Here's my experience with transparent mycelial growth.
This which I thought was mycelium. Turned into this: 
Your mileage may vary.
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The Mycologist
Explorer

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Re: Transparent mycelium on agar [Re: Tiamo]
#24003318 - 01/12/17 07:51 AM (7 years, 18 days ago) |
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Looks like some bacteria riding with the mycelium
-------------------- "That you are here—that life exists, and identity; That the powerful play goes on, and you will contribute a verse.” ― Walt Whitman, Leaves of Grass

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Kenetic
Nam Sayin



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Normal
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Psilosopherr
A psilly goose



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Re: Transparent mycelium on agar [Re: Kenetic]
#24003397 - 01/12/17 08:38 AM (7 years, 18 days ago) |
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70 degrees is well within their range as I'm sure you know. Its not thatt fragile
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Crispykoot
Jello Wrangler



Registered: 10/16/16
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I've run the plates that these came from...All the grain worked out fine up to G2, would have gone to G3, and fruiting has been 'normal'. Thus is why I don't think its bacterial...
Kenetic...you think this is normal then? I transferred from the leading edge as usual, which was pretty transparent. I've seen myc transforming a lot and a lot of zonate type which I was told was a temp thing.
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Kenetic
Nam Sayin



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Re: Transparent mycelium on agar [Re: Crispykoot]
#24003624 - 01/12/17 10:13 AM (7 years, 18 days ago) |
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Every single plate Ive ever had has had a leading edge that was clear. Itll turn white soon.
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Psilosoulful
Registered: 09/05/14
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Re: Transparent mycelium on agar [Re: Kenetic]
#24003638 - 01/12/17 10:19 AM (7 years, 18 days ago) |
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It could likely be the nutrient profile of the agar itself that is causing the mycelium to show that kind of growth. Try to tweak your recipe a bit next time
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Crispykoot
Jello Wrangler



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Quote:
Psilosoulful said: It could likely be the nutrient profile of the agar itself that is causing the mycelium to show that kind of growth. Try to tweak your recipe a bit next time
I think that could be a good call... The plates in question were transferred from plates in the fridge...
Here's 2 photos of transfers taken a day apart. One is from cold storage and MS, one is a clone, never in cold storage. Both on the same medium. I did change the agar recipe slightly from the stuff in cold storage by reducing the malt extract content. Made everything else go more rhizo...

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Psilosoulful
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Re: Transparent mycelium on agar [Re: Crispykoot]
#24005010 - 01/12/17 06:04 PM (7 years, 18 days ago) |
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Nice!!!
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