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Tiamo
Trust in LITFA




Registered: 04/07/16
Posts: 1,935
Loc: Amsterdam
Last seen: 10 hours, 30 minutes
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Getting so frustrated
#24002967 - 01/12/17 01:36 AM (7 years, 19 days ago) |
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I just want to vent for a second because I'm mad as hell. I've been doing a lot of agar, and I mean a lot. I've gone through 66 petri dishes in a month and I've only seen 2 make it, a Reishi plate and an AA+ plate. I've lost all of my Ps. cyanescens plates because a bacterial infection showed up just on the rim of the plate. No biggie, I thought and transferred it, but then all the plates became bacterial and had to be tossed. I've tried again and again to start from spores on agar and they all just contaminate within 24 hours. I've had bad pours, plate never solidify, bad media, bad spores, moulds, bacteria, whatever you can imagine.
And then, another thing, everthing is SLOW AS HELL. Agar growth is slowest, even moulds are slow! I figure this is down to my recipe. I have a very poor MEA premix from eBay and I HATE it.
Recipe:
Peptone 4g Yeast 3g Agar Agar 13g Glucose 10g Malt 10g
Today is the day I finally get agar agar to mix my own.
6 days ago, I used a wedge of that "succesful" AA+ to inoculate a grain jar 6 days ago and shook it, the agar and mycelium disappeared and a few days later I had growth on a few dozen kernels but they haven't progressed much, just a few millimeters. Not surprising considering it did exactly the same on the petris. Everyone says it takes 2 weeks to colonise a Qt of rye? My arse it does.
The Reishi I made a LC with and inoculated 3 grain jars, also 6 days ago, and I can only spot about 10 kernels between the 3 of them that have any mycelium on them. The LC looked clean as fuck and colonised in 9 days and it's this slow? My PF cakes are also colonising very slowly. It easily takes 7 weeks+ for them to get going to 100%.
I just don't understand and it's very hard not to get discouraged. I'm doing everything right. I've got a SAB, I practice sterile procedure. I make petris and use them to practice inoculation by not actually swiping spores and they never contaminate. My room is heated. It's 20 C during the day and drops to 17 C at night. It's very frustrating.
Sorry, just needed to get that out. I've been trying desperately since December to get any successful growth on agar at all. And when I do, it's so fucking slow it isn't even worth talking about. I thought 2017 would be the year that I got clean plates and clean spawn with fast colonising rye jars and lovely PF cakes and then it all just fell apart. 
At least I don't get contamination in the rye or the cakes anymore, just on the plates.
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If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
Edited by Tiamo (01/12/17 01:43 AM)
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,379
Loc: The Inexpressible...
Last seen: 9 minutes, 38 seconds
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Re: Getting so frustrated [Re: Tiamo]
#24003002 - 01/12/17 02:05 AM (7 years, 19 days ago) |
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Not sure i can help but i have heard of antibacteria agar. Maybe thats what u need.
Also isnt 20c kinda cold? Should around 60 to 70F for best growth. Cold temps means slow growth.
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Tiamo
Trust in LITFA




Registered: 04/07/16
Posts: 1,935
Loc: Amsterdam
Last seen: 10 hours, 30 minutes
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20 C = 68 F
--------------------
If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,379
Loc: The Inexpressible...
Last seen: 9 minutes, 38 seconds
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Re: Getting so frustrated [Re: Tiamo]
#24003009 - 01/12/17 02:14 AM (7 years, 19 days ago) |
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enlightenment
alchemist


Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 8 months, 22 days
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Re: Getting so frustrated [Re: Tiamo]
#24003014 - 01/12/17 02:18 AM (7 years, 19 days ago) |
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Cheer up! Agar gets contaminated easily. Think about your sterile technique. I am sure there is something you can improve.
Look at Bodhisatta's SAB TEK. Take a look at my agar recipe.
Try to work fast and clean.
Do you use pasty plates (pre-poured) or petri dishes?
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Tiamo
Trust in LITFA




Registered: 04/07/16
Posts: 1,935
Loc: Amsterdam
Last seen: 10 hours, 30 minutes
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Yea, the pre-mix I mentioned is bullshit. I ordered agar agar off eBay to mix for myself in December and it should be here any day now. But meanwhile I also found agar agar in my town for cheaper and I already ordered it, I can probably pick it up today or tomorrow. I can't wait to bin this pre-mix. Half of the time it wouldn't solidify and it's soft as balls. I think that's where a lot of my problems stem from.
@enlightenment I use petri dishes, I lift the cover up under a 45 degree angle and stick my inoculation loop in for a second. I never open it for more than 5 seconds in a SAB. I do practice runs with agar in my SAB to test my sterile procedure. It's clean ~90% of the time.
--------------------
If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,379
Loc: The Inexpressible...
Last seen: 9 minutes, 38 seconds
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Re: Getting so frustrated [Re: Tiamo]
#24003019 - 01/12/17 02:24 AM (7 years, 19 days ago) |
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One thing u can do is make a couple agar plate and dont touch them! Make it a control and watch for containments.
That way u can determine if its the agar itself or your inoculation loop/SAB.
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Tiamo
Trust in LITFA




Registered: 04/07/16
Posts: 1,935
Loc: Amsterdam
Last seen: 10 hours, 30 minutes
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Well guys. I just made my own mix. I hope this will fix all my problems in life. Behold the glorious pink agar. 100 mL water, 2 grams agar, 2 grams BRF, one drop red food colouring.

Hell yes.
--------------------
If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
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Psilosoulful
Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Getting so frustrated [Re: Tiamo]
#24003507 - 01/12/17 09:32 AM (7 years, 18 days ago) |
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