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Adizzledog
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Registered: 01/09/17
Posts: 27
Last seen: 6 years, 9 months
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Newbie Grower 2nd try
#24001794 - 01/11/17 05:20 PM (7 years, 19 days ago) |
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Hey guys!
So my buddy and I decided to take the plunge together into the world of mycology. He's done Successful PF teks before so we decided to go straight into RR's rye grain bulk grow tek.
Well we didn't do enough researching and ended up using some outdated techniques and managed to grow some nice trich for our first batch . We bought premade jars (1st mistake), used a glove box (2nd mistake), and incubated at 80F X_X.
So we did a lot of reading of this forum and went round 2 this last weekend. Made our own jars, used a SAB, got anal about sterilization, and no incubation this time. Praying to the shroom gods for a good batch.
Anyways, I'm hoping if I run into any problems/questions/concerns I could ask them here as well as keep you guys updated on how everything is going?
Thanks homies!
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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Re: Newbie Grower 2nd try [Re: Adizzledog]
#24001823 - 01/11/17 05:33 PM (7 years, 19 days ago) |
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Welcome aboard!
If you want to go into detail about your sterile technique when working inside the SAB, I can review it for ya.
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VENOM
DEATH 2 SPIDERMAN


Registered: 01/11/17
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Re: Newbie Grower 2nd try [Re: Adizzledog]
#24001835 - 01/11/17 05:39 PM (7 years, 19 days ago) |
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Good Luck!
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Adizzledog
Stranger
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Re: Newbie Grower 2nd try [Re: Munchauzen] 1
#24001946 - 01/11/17 06:16 PM (7 years, 19 days ago) |
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Thanks!
So we followed SpitballJedi's guide on the SAB, 108 qt clear bin, holes drilled, used a towel on bottom as a gasket.
We used my office with everything taken out of it and completely disinfected. I'm talking 10% bleach on the floor and surfaces, Clorox disinfectant wipes for the walls and isopropanol for everything around and inside the SAB (table and all items used).
We put on fresh clothes and sat motionless in office for 5 minutes to allow everything to settle. Then prepared the syringe, flame sterilized the needle outside the box, wiped with alcohol wipe inside, poke through the self healing port on first jar, squirted 1.5cc around the sides of the jar, rinse and repeat.
All in all I felt good about the process. I always have a creeping anxiety that I didn't do something right, but we were much more diligent this time, so (knocking on wood) hopefully no mold this time.
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Pipefitter537
You're Not Yelping



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Re: Newbie Grower 2nd try [Re: Adizzledog]
#24001966 - 01/11/17 06:21 PM (7 years, 19 days ago) |
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Did you alcohol wipe the needle after flame sterilizing? If so that might be an issue.
-------------------- "You should never underestimate the predictability of stupidity"-Bullet Tooth Tony
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Adizzledog
Stranger
Registered: 01/09/17
Posts: 27
Last seen: 6 years, 9 months
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How so? I can't remember where I read it but someone said it was good to cool the needle down. Plus I wiped the ports down with the wipe directly after so I don't see how it could hurt.
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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Re: Newbie Grower 2nd try [Re: Adizzledog]
#24002043 - 01/11/17 06:40 PM (7 years, 19 days ago) |
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Quote:
Adizzledog said: Thanks!
So we followed SpitballJedi's guide on the SAB, 108 qt clear bin, holes drilled, used a towel on bottom as a gasket.
We used my office with everything taken out of it and completely disinfected. I'm talking 10% bleach on the floor and surfaces, Clorox disinfectant wipes for the walls and isopropanol for everything around and inside the SAB (table and all items used).
We put on fresh clothes and sat motionless in office for 5 minutes to allow everything to settle. Then prepared the syringe, flame sterilized the needle outside the box, wiped with alcohol wipe inside, poke through the self healing port on first jar, squirted 1.5cc around the sides of the jar, rinse and repeat.
All in all I felt good about the process. I always have a creeping anxiety that I didn't do something right, but we were much more diligent this time, so (knocking on wood) hopefully no mold this time.
Ok thats a bit overkill. For reference, I do all my SAB work inside a carpetted walk-in closet in my bedroom. I don't scrub the walls or anything... I just prepare my sab and work.
Isopropyl alcohol should be avoided inside your SAB. Its a still air box, not a sterile air box. The walls inside the SAB need to remain wet as well, and isopropyl alcohol evaporates very quickly. The only thing you should be spraying inside your SAB is water with a drop of dish soap. This is the basic principle of the SAB, contams get caught inside the water droplets on your walls, instead of bouncing off. The drop of dish soap helps reduce the surface tension of the water so that it grabs onto the floating contaminates better. Also, we've had more than one user have their SAB explode as the inside filled with alcohol vapor and then they did flame sterilization and boom! don't want any hospital visits for you.
Definitely never wipe a syringe with alcohol. Flame sterilization 100%. Flames sterilize, alcohol sanitizes. The famous line around here is that wiping after flaming is like smearing shit on your face after taking a shower.
Also, you shouldn't be squirting spores on grains. That method is likely to contaminate because spores are never 100% clean. You should be making agar dishes aka pasty plates to grow and clean your cultures on. Only after you have a clean culture, do you put that on your sterilized grains.
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Adizzledog
Stranger
Registered: 01/09/17
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Last seen: 6 years, 9 months
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Re: Newbie Grower 2nd try [Re: Munchauzen]
#24002085 - 01/11/17 06:50 PM (7 years, 19 days ago) |
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Quote:
Munchauzen said: Ok thats a bit overkill. For reference, I do all my SAB work inside a carpetted walk-in closet in my bedroom. I don't scrub the walls or anything... I just prepare my sab and work.
Isopropyl alcohol should be avoided inside your SAB. Its a still air box, not a sterile air box. The walls inside the SAB need to remain wet as well, and isopropyl alcohol evaporates very quickly. The only thing you should be spraying inside your SAB is water with a drop of dish soap. This is the basic principle of the SAB, contams get caught inside the water droplets on your walls, instead of bouncing off. The drop of dish soap helps reduce the surface tension of the water so that it grabs onto the floating contaminates better. Also, we've had more than one user have their SAB explode as the inside filled with alcohol vapor and then they did flame sterilization and boom! don't want any hospital visits for you.
Definitely never wipe a syringe with alcohol. Flame sterilization 100%. Flames sterilize, alcohol sanitizes. The famous line around here is that wiping after flaming is like smearing shit on your face after taking a shower.
Also, you shouldn't be squirting spores on grains. That method is likely to contaminate because spores are never 100% clean. You should be making agar dishes aka pasty plates to grow and clean your cultures on. Only after you have a clean culture, do you put that on your sterilized grains.
Well damn looks like I still have a lot to learn. Looking into agar tek after this. So you're thinking I'll likely have contams again?
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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Re: Newbie Grower 2nd try [Re: Adizzledog]
#24002101 - 01/11/17 06:55 PM (7 years, 19 days ago) |
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Quote:
Adizzledog said:
Quote:
Munchauzen said: Ok thats a bit overkill. For reference, I do all my SAB work inside a carpetted walk-in closet in my bedroom. I don't scrub the walls or anything... I just prepare my sab and work.
Isopropyl alcohol should be avoided inside your SAB. Its a still air box, not a sterile air box. The walls inside the SAB need to remain wet as well, and isopropyl alcohol evaporates very quickly. The only thing you should be spraying inside your SAB is water with a drop of dish soap. This is the basic principle of the SAB, contams get caught inside the water droplets on your walls, instead of bouncing off. The drop of dish soap helps reduce the surface tension of the water so that it grabs onto the floating contaminates better. Also, we've had more than one user have their SAB explode as the inside filled with alcohol vapor and then they did flame sterilization and boom! don't want any hospital visits for you.
Definitely never wipe a syringe with alcohol. Flame sterilization 100%. Flames sterilize, alcohol sanitizes. The famous line around here is that wiping after flaming is like smearing shit on your face after taking a shower.
Also, you shouldn't be squirting spores on grains. That method is likely to contaminate because spores are never 100% clean. You should be making agar dishes aka pasty plates to grow and clean your cultures on. Only after you have a clean culture, do you put that on your sterilized grains.
Well damn looks like I still have a lot to learn. Looking into agar tek after this. So you're thinking I'll likely have contams again?
its possible. its also possible you see the grow thru to finish and get fruits. spores to grains isn't guaranteed failure, its just not the best practice when you have the tools to do better. you said you made your own grain jars, so I know that means you have to have a pressure cooker. and if you have a pressure cooker then you can do pastywhyte's easy agar tek. the only hard part of that tek is finding the agar. whole foods and asain markets sell it, otherwise you will need to order it off amazon.
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Adizzledog
Stranger
Registered: 01/09/17
Posts: 27
Last seen: 6 years, 9 months
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Re: Newbie Grower 2nd try [Re: Munchauzen]
#24002121 - 01/11/17 07:04 PM (7 years, 19 days ago) |
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Ok thanks for the shred of hope. Yes we have a PC, I'll go looking for agar at the whole foods by work tomorrow. I'll keep you updated as things grow. Thanks for all your help!
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Psilosoulful
Registered: 09/05/14
Posts: 7,205
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Re: Newbie Grower 2nd try [Re: Adizzledog]
#24002642 - 01/11/17 10:46 PM (7 years, 19 days ago) |
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Ace187
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Registered: 01/12/17
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I have a question! When I pressure cook my jars - they are completely sealed shut. After I inoculate am I supposed to leave the holes open but cover them with foil to allow the gas to exchange? I'm confused on this one aspect. After i inoculate I thought it was supposed to be air tight sealed? Or do I leave the holes from the syringe open to allow gas exchange plus a little breather hole?
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NDStepp84
Stellar nuclear waste

Registered: 04/23/15
Posts: 4,956
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Re: Newbie Grower 2nd try [Re: Ace187] 1
#24003087 - 01/12/17 03:39 AM (7 years, 18 days ago) |
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You are supposed to have a filter, it's the dry verm layer in PF tek.
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"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do. -Robert A. Heinlein
Links and teks ND's grow log and discussion Plant thread
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
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Re: Newbie Grower 2nd try [Re: Ace187]
#24003114 - 01/12/17 04:15 AM (7 years, 18 days ago) |
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PF jars use the verm as a barrier.
Grain jars use a filter such as polyfill or tyvek. Gas exchange is very important for spawning for both types of jars.
-------------------- "What you must understand is that your physical dimension affects everyone in the higher dimensions as well. All things are interconnected. All things are One. Therefore, if one dimension is broken or out of balance, then all other dimensions will experience repercussions." - Pleiadian Prophecy 2020 The New Golden Age by James Carwin PROJECT BLUE BOOK ANALYSIS! (312 pages!) | Psychedelics & UFOs | Ready to Contact UFOs? | The Source on Mushrooms | Trippy Gematrix | Dj TeknoLogical | Fentanyl Test Kits R.I.P. Big Worm || The Start of the Ascension Process was 2020. Welcome to the Next Great Era of Earth 🌎🌍🌏
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Adizzledog
Stranger
Registered: 01/09/17
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Last seen: 6 years, 9 months
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So we're 10 days in and seeing signs of growth on our jars.
EDIT: pics below
How we lookin?
Also started some agar cups
Inoced last Friday, not seeing anything yet. Should I be concerned about the amount of condensation?
Edited by Adizzledog (01/18/17 04:21 PM)
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Terpfreak
❀Terpenes❀



Registered: 07/08/16
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Re: Newbie Grower 2nd try [Re: Adizzledog]
#24019910 - 01/18/17 12:56 PM (7 years, 12 days ago) |
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Condensation is normal.
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mushboy
modboy



Registered: 04/24/05
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Re: Newbie Grower 2nd try [Re: Terpfreak]
#24019917 - 01/18/17 12:59 PM (7 years, 12 days ago) |
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flick the sides of the containers rather hard with your finger. dont be gentle. in a day condensation is no more.
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Adizzledog
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Re: Newbie Grower 2nd try [Re: mushboy]
#24020412 - 01/18/17 03:33 PM (7 years, 12 days ago) |
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Good to know thanks!
How about my jars? Look like clean myc?
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TheDuder
Mushroom Hunter



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Re: Newbie Grower 2nd try [Re: Adizzledog]
#24020495 - 01/18/17 03:57 PM (7 years, 12 days ago) |
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Looks good to me, keep it up
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|-------------------[Ps. Azurescens]------------------------------------------[Ps. Semilanceata]--------------------------------------------[Ps. Allenii]------------------------|
|--------------[Ps. Ovoideocystidiata]------------------------------------------[Ps. Stuntzii]--------------------------------------------[Ps. Baeocystis]----------------------|
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Adizzledog
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Re: Newbie Grower 2nd try [Re: TheDuder]
#24020656 - 01/18/17 04:53 PM (7 years, 12 days ago) |
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Awesome, that's what I like to hear. Gonna do another round of agar plates this weekend. Not feeling too confident about my first round. Wish me luck! Btw, you guys are great. Thanks for all the help!
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Adizzledog
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Went a little crazy with plates.

Have growth on my first round. Looks healthy, although a bit puffy. Is that ok? Can't really take a good pic through the cups.
I know most people do at least 4 transfers until they drop in grain, but what is the absolute minimum transfers I need to do before I drop? Is isolation really important, or can I just worry about making sure there is no bacteria.
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Psilosoulful

Registered: 09/05/14
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Nice plates I usually do a minimum of 2 transfers before dropping a clean outer edge piece of clean mycelium to my grain jars
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Adizzledog
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Registered: 01/09/17
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Last seen: 6 years, 9 months
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Things are going nicely so far. Here are my favorite cups from my first round.
    
I have one that is showing some bacteria, would you guys still transfer off of this one, or just toss. I've got plenty more so I'm not too worried. (It's around 10/11 o'clock position)

And my jars are looking nice! How's this for beautiful rhizomorphic growth? It's been 3 weeks since inoculation.
     
You guys think they're ready for shaking?
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LogicaL Chaos
Ascension Energy & Alien UFOs




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Nice work!
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Adizzledog
Stranger

Registered: 01/09/17
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Last seen: 6 years, 9 months
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Hey guys
Just wanted to update my thread on where I'm at so far.
So I have 5 out of the 6 original jars (syringe straight to grain) in a tub currently. Don't have super high hopes for it because I did a shake test on the 6th jar, hoping that if it was ok I would use for g2g. This is what it looks like on day 3.
   
What do you guys think? Definitely bacterial right?
Not super worried though because I have some agar plates that look pretty nice that I'm going to drop on grain today. Here they are.
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
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Re: Newbie Grower 2nd try [Re: Adizzledog]
#24086455 - 02/13/17 03:41 AM (6 years, 11 months ago) |
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your grain jars look decent but something looks off about them. Not sure what it is
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Adizzledog
Stranger

Registered: 01/09/17
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Sorry I should've been more clear. Those pictures are all of one jar, the one I was hoping to use for g2g. 3 days after a shake test. I already spawned the others. There's gotta be something bacterial in there. If you look at the 2nd pic, you can see 3 thick blob things on top of the grain. That and the fact that it hasn't fully recovered in 3 days. Just looking for confirmation that I'm not crazy or stupid lol.
Anyways, it'd be great if I got any yield from this batch, but won't be devastated if I don't. Thanks to you guys I have many more chances at this.
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Adizzledog
Stranger

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Re: Newbie Grower 2nd try [Re: Adizzledog]
#24097606 - 02/17/17 11:23 AM (6 years, 11 months ago) |
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Instead of continuing to update this thread im gonna put everything in my journal from now on. Just in case any of you wanna follow along
https://www.shroomery.org/forums/showflat.php/Number/24097589
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