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OfflineAdizzledog
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Registered: 01/09/17
Posts: 27
Last seen: 6 years, 9 months
Newbie Grower 2nd try
    #24001794 - 01/11/17 05:20 PM (7 years, 19 days ago)

Hey guys!

So my buddy and I decided to take the plunge together into the world of mycology. He's done Successful PF teks before so we decided to go straight into RR's rye grain bulk grow tek.

Well we didn't do enough researching and ended up using some outdated techniques and managed to grow some nice trich for our first batch :frown:. We bought premade jars (1st mistake), used a glove box (2nd mistake), and incubated at 80F X_X.

So we did a lot of reading of this forum and went round 2 this last weekend. Made our own jars, used a SAB, got anal about sterilization, and no incubation this time. Praying to the shroom gods for a good batch.

Anyways, I'm hoping if I run into any problems/questions/concerns I could ask them here as well as keep you guys updated on how everything is going?

Thanks homies!


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InvisibleMunchauzen
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Registered: 06/22/11
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Re: Newbie Grower 2nd try [Re: Adizzledog]
    #24001823 - 01/11/17 05:33 PM (7 years, 19 days ago)

Welcome aboard!

If you want to go into detail about your sterile technique when working inside the SAB, I can review it for ya.


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OfflineVENOM
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Re: Newbie Grower 2nd try [Re: Adizzledog]
    #24001835 - 01/11/17 05:39 PM (7 years, 19 days ago)

Good Luck!


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OfflineAdizzledog
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Re: Newbie Grower 2nd try [Re: Munchauzen] * 1
    #24001946 - 01/11/17 06:16 PM (7 years, 19 days ago)

Thanks!

So we followed SpitballJedi's guide on the SAB, 108 qt clear bin, holes drilled, used a towel on bottom as a gasket.

We used my office with everything taken out of it and completely disinfected. I'm talking 10% bleach on the floor and surfaces, Clorox disinfectant wipes for the walls and isopropanol for everything around and inside the SAB (table and all items used).

We put on fresh clothes and sat motionless in office for 5 minutes to allow everything to settle. Then prepared the syringe, flame sterilized the needle outside the box, wiped with alcohol wipe inside, poke through the self healing port on first jar, squirted 1.5cc around the sides of the jar, rinse and repeat.

All in all I felt good about the process. I always have a creeping anxiety that I didn't do something right, but we were much more diligent this time, so (knocking on wood) hopefully no mold this time.


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InvisiblePipefitter537
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Re: Newbie Grower 2nd try [Re: Adizzledog]
    #24001966 - 01/11/17 06:21 PM (7 years, 19 days ago)

Did you alcohol wipe the needle after flame sterilizing?  If so that might be an issue.


--------------------
"You should never underestimate the predictability of stupidity"-Bullet Tooth Tony




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OfflineAdizzledog
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Last seen: 6 years, 9 months
Re: Newbie Grower 2nd try [Re: Pipefitter537]
    #24002002 - 01/11/17 06:32 PM (7 years, 19 days ago)

How so? I can't remember where I read it but someone said it was good to cool the needle down. Plus I wiped the ports down with the wipe directly after so I don't see how it could hurt.


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InvisibleMunchauzen
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Re: Newbie Grower 2nd try [Re: Adizzledog]
    #24002043 - 01/11/17 06:40 PM (7 years, 19 days ago)

Quote:

Adizzledog said:
Thanks!

So we followed SpitballJedi's guide on the SAB, 108 qt clear bin, holes drilled, used a towel on bottom as a gasket.

We used my office with everything taken out of it and completely disinfected. I'm talking 10% bleach on the floor and surfaces, Clorox disinfectant wipes for the walls and isopropanol for everything around and inside the SAB (table and all items used).

We put on fresh clothes and sat motionless in office for 5 minutes to allow everything to settle. Then prepared the syringe, flame sterilized the needle outside the box, wiped with alcohol wipe inside, poke through the self healing port on first jar, squirted 1.5cc around the sides of the jar, rinse and repeat.

All in all I felt good about the process. I always have a creeping anxiety that I didn't do something right, but we were much more diligent this time, so (knocking on wood) hopefully no mold this time.



Ok thats a bit overkill. For reference, I do all my SAB work inside a carpetted walk-in closet in my bedroom. I don't scrub the walls or anything... I just prepare my sab and work.

Isopropyl alcohol should be avoided inside your SAB. Its a still air box, not a sterile air box. The walls inside the SAB need to remain wet as well, and isopropyl alcohol evaporates very quickly. The only thing you should be spraying inside your SAB is water with a drop of dish soap. This is the basic principle of the SAB, contams get caught inside the water droplets on your walls, instead of bouncing off. The drop of dish soap helps reduce the surface tension of the water so that it grabs onto the floating contaminates better. Also, we've had more than one user have their SAB explode as the inside filled with alcohol vapor and then they did flame sterilization and boom! don't want any hospital visits for you.

Definitely never wipe a syringe with alcohol. Flame sterilization 100%. Flames sterilize, alcohol sanitizes. The famous line around here is that wiping after flaming is like smearing shit on your face after taking a shower.

Also, you shouldn't be squirting spores on grains. That method is likely to contaminate because spores are never 100% clean. You should be making agar dishes aka pasty plates to grow and clean your cultures on. Only after you have a clean culture, do you put that on your sterilized grains.


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OfflineAdizzledog
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Registered: 01/09/17
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Re: Newbie Grower 2nd try [Re: Munchauzen]
    #24002085 - 01/11/17 06:50 PM (7 years, 19 days ago)

Quote:

Munchauzen said:
Ok thats a bit overkill. For reference, I do all my SAB work inside a carpetted walk-in closet in my bedroom. I don't scrub the walls or anything... I just prepare my sab and work.

Isopropyl alcohol should be avoided inside your SAB. Its a still air box, not a sterile air box. The walls inside the SAB need to remain wet as well, and isopropyl alcohol evaporates very quickly. The only thing you should be spraying inside your SAB is water with a drop of dish soap. This is the basic principle of the SAB, contams get caught inside the water droplets on your walls, instead of bouncing off. The drop of dish soap helps reduce the surface tension of the water so that it grabs onto the floating contaminates better. Also, we've had more than one user have their SAB explode as the inside filled with alcohol vapor and then they did flame sterilization and boom! don't want any hospital visits for you.

Definitely never wipe a syringe with alcohol. Flame sterilization 100%. Flames sterilize, alcohol sanitizes. The famous line around here is that wiping after flaming is like smearing shit on your face after taking a shower.

Also, you shouldn't be squirting spores on grains. That method is likely to contaminate because spores are never 100% clean. You should be making agar dishes aka pasty plates to grow and clean your cultures on. Only after you have a clean culture, do you put that on your sterilized grains.




Well damn looks like I still have a lot to learn. Looking into agar tek after this. So you're thinking I'll likely have contams again?


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InvisibleMunchauzen
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Re: Newbie Grower 2nd try [Re: Adizzledog]
    #24002101 - 01/11/17 06:55 PM (7 years, 19 days ago)

Quote:

Adizzledog said:
Quote:

Munchauzen said:
Ok thats a bit overkill. For reference, I do all my SAB work inside a carpetted walk-in closet in my bedroom. I don't scrub the walls or anything... I just prepare my sab and work.

Isopropyl alcohol should be avoided inside your SAB. Its a still air box, not a sterile air box. The walls inside the SAB need to remain wet as well, and isopropyl alcohol evaporates very quickly. The only thing you should be spraying inside your SAB is water with a drop of dish soap. This is the basic principle of the SAB, contams get caught inside the water droplets on your walls, instead of bouncing off. The drop of dish soap helps reduce the surface tension of the water so that it grabs onto the floating contaminates better. Also, we've had more than one user have their SAB explode as the inside filled with alcohol vapor and then they did flame sterilization and boom! don't want any hospital visits for you.

Definitely never wipe a syringe with alcohol. Flame sterilization 100%. Flames sterilize, alcohol sanitizes. The famous line around here is that wiping after flaming is like smearing shit on your face after taking a shower.

Also, you shouldn't be squirting spores on grains. That method is likely to contaminate because spores are never 100% clean. You should be making agar dishes aka pasty plates to grow and clean your cultures on. Only after you have a clean culture, do you put that on your sterilized grains.




Well damn looks like I still have a lot to learn. Looking into agar tek after this. So you're thinking I'll likely have contams again?



its possible. its also possible you see the grow thru to finish and get fruits. spores to grains isn't guaranteed failure, its just not the best practice when you have the tools to do better. you said you made your own grain jars, so I know that means you have to have a pressure cooker. and if you have a pressure cooker then you can do pastywhyte's easy agar tek. the only hard part of that tek is finding the agar. whole foods and asain markets sell it, otherwise you will need to order it off amazon.


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OfflineAdizzledog
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Last seen: 6 years, 9 months
Re: Newbie Grower 2nd try [Re: Munchauzen]
    #24002121 - 01/11/17 07:04 PM (7 years, 19 days ago)

Ok thanks for the shred of hope. Yes we have a PC, I'll go looking for agar at the whole foods by work tomorrow. I'll keep you updated as things grow. Thanks for all your help!


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OfflinePsilosoulful
Registered: 09/05/14
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Re: Newbie Grower 2nd try [Re: Adizzledog]
    #24002642 - 01/11/17 10:46 PM (7 years, 19 days ago)

:levitate:


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OfflineAce187
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Registered: 01/12/17
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Last seen: 6 years, 10 months
Re: Newbie Grower 2nd try [Re: Psilosoulful]
    #24003046 - 01/12/17 02:46 AM (7 years, 18 days ago)

I have a question!  When I pressure cook my jars - they are completely sealed shut. After I inoculate am I supposed to leave the holes open but cover them with foil to allow the gas to exchange? I'm confused on this one aspect. After i inoculate I thought it was supposed to be air tight sealed? Or do I leave the holes from the syringe open to allow gas exchange plus a little breather hole?


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OfflineNDStepp84
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Re: Newbie Grower 2nd try [Re: Ace187] * 1
    #24003087 - 01/12/17 03:39 AM (7 years, 18 days ago)

:scumbagman:  You are supposed to have a filter, it's the dry verm layer in PF tek.


--------------------

"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
-Robert A. Heinlein 

:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread


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OfflineLogicaL ChaosM
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Re: Newbie Grower 2nd try [Re: Ace187]
    #24003114 - 01/12/17 04:15 AM (7 years, 18 days ago)

PF jars use the verm as a barrier.

Grain jars use a filter such as polyfill or tyvek. Gas exchange is very important for spawning for both types of jars.


--------------------
"What you must understand is that your physical dimension affects everyone in the higher dimensions as well. All things are interconnected. All things are One. Therefore, if one dimension is broken or out of balance, then all other dimensions will experience repercussions." - Pleiadian Prophecy 2020 The New Golden Age by James Carwin

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OfflineAdizzledog
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Re: Newbie Grower 2nd try [Re: LogicaL Chaos]
    #24019655 - 01/18/17 11:03 AM (7 years, 12 days ago)

So we're 10 days in and seeing signs of growth on our jars.

EDIT: pics below

How we lookin?

Also started some agar cups



Inoced last Friday, not seeing anything yet. Should I be concerned about the amount of condensation?


Edited by Adizzledog (01/18/17 04:21 PM)


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OfflineTerpfreak
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Re: Newbie Grower 2nd try [Re: Adizzledog]
    #24019910 - 01/18/17 12:56 PM (7 years, 12 days ago)

Condensation is normal.


--------------------


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InvisiblemushboyMDiscord
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Re: Newbie Grower 2nd try [Re: Terpfreak]
    #24019917 - 01/18/17 12:59 PM (7 years, 12 days ago)

flick the sides of the containers rather hard with your finger. dont be gentle. in a day condensation is no more.


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OfflineAdizzledog
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Re: Newbie Grower 2nd try [Re: mushboy]
    #24020412 - 01/18/17 03:33 PM (7 years, 12 days ago)

Good to know thanks!

How about my jars? Look like clean myc?



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OfflineTheDuder
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Re: Newbie Grower 2nd try [Re: Adizzledog]
    #24020495 - 01/18/17 03:57 PM (7 years, 12 days ago)

Looks good to me, keep it up :rockon:


--------------------

|-------------------[Ps. Azurescens]------------------------------------------[Ps. Semilanceata]--------------------------------------------[Ps. Allenii]------------------------|


|--------------[Ps. Ovoideocystidiata]------------------------------------------[Ps. Stuntzii]--------------------------------------------[Ps. Baeocystis]----------------------|


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OfflineAdizzledog
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Re: Newbie Grower 2nd try [Re: TheDuder]
    #24020656 - 01/18/17 04:53 PM (7 years, 12 days ago)

Awesome, that's what I like to hear. Gonna do another round of agar plates this weekend. Not feeling too confident about my first round. Wish me luck! Btw, you guys are great. Thanks for all the help!


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