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Offlinemushhiehunter
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cyanescens spore syringe black??
    #24000190 - 01/11/17 04:02 AM (7 years, 19 days ago)

Hey guys so I bought a spore syringe with p. cyanescens spores from a EU vendor and to my surprise it has dark liquid in it. Now I suspect it being a dye since the amount of spores needed for such a discoloration would be massive... What do you guys think??


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Re: cyanescens spore syringe black?? [Re: mushhiehunter]
    #24000194 - 01/11/17 04:06 AM (7 years, 19 days ago)

Yea, that looks like someone dyed the water in it. :facepalm: I hate when vendors do this.


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Re: cyanescens spore syringe black?? [Re: Tiamo]
    #24000199 - 01/11/17 04:10 AM (7 years, 19 days ago)

Quote:

Tiamo said:
Yea, that looks like someone dyed the water in it. :facepalm: I hate when vendors do this.




Well maybe your average Joe feels better when he sees he's not buying clear water... just imagine the number of spores needed for this effect though... it would be possibly more than all humans that ever lived


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Re: cyanescens spore syringe black?? [Re: mushhiehunter]
    #24000306 - 01/11/17 06:07 AM (7 years, 19 days ago)

.


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Edited by amidogen (04/19/18 01:14 PM)


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Re: cyanescens spore syringe black?? [Re: amidogen]
    #24000325 - 01/11/17 06:22 AM (7 years, 19 days ago)

Quote:

amidogen said:
Quote:

mushhiehunter said:
Quote:

Tiamo said:
Yea, that looks like someone dyed the water in it. :facepalm: I hate when vendors do this.




Well maybe your average Joe feels better when he sees he's not buying clear water... just imagine the number of spores needed for this effect though... it would be possibly more than all humans that ever lived



:facepalm:

That's the point...they dye the water with coloring so you can't see how few spores they gave you. If I got a syringe like this, I'd assume it's nearly empty or completely empty of viable spores. A reputable vendor with quality syringes will give you one without dye and you'll see the large black clumps of spores floating in the water. I'd ask for a refund. The spores are for microscopy right? How the fuck are you supposed to see black spores suspended in black water? Maybe you'll have luck with it but my bet is it's not a viable syringe. Sorry man.




I know that the discoloration doesn't come from the spores, I was just thinking out lout how many actual spores it would take to get to this effect :laugh:


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Re: cyanescens spore syringe black?? [Re: mushhiehunter]
    #24000340 - 01/11/17 06:41 AM (7 years, 19 days ago)

yeah that seems like bullshit... have ya shot a drop on a plate yet?


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Re: cyanescens spore syringe black?? [Re: synthetik]
    #24000342 - 01/11/17 06:42 AM (7 years, 19 days ago)

Quote:

synthetik said:
yeah that seems like bullshit... have ya shot a drop on a plate yet?




Nope, but I sent them a heated e-mail this morning.. we'll see what comes out of it....


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Re: cyanescens spore syringe black?? [Re: mushhiehunter]
    #24000379 - 01/11/17 07:07 AM (7 years, 19 days ago)

They put carbon in it aparrently to protect the spores from bacteria. They should work fine


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Re: cyanescens spore syringe black?? [Re: Unknown error]
    #24000408 - 01/11/17 07:35 AM (7 years, 19 days ago)

They don't do it to protect the spores, they do it so their product looks better.


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Re: cyanescens spore syringe black?? [Re: Unknown error]
    #24000411 - 01/11/17 07:40 AM (7 years, 19 days ago)

.


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Edited by amidogen (04/19/18 01:14 PM)


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Re: cyanescens spore syringe black?? [Re: Tiamo]
    #24000418 - 01/11/17 07:45 AM (7 years, 19 days ago)

Just what they said when I got mine. Had no problems


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Re: cyanescens spore syringe black?? [Re: amidogen]
    #24000478 - 01/11/17 08:24 AM (7 years, 19 days ago)

Quote:

Unknown error said:
They put carbon in it aparrently to protect the spores from bacteria. They should work fine[/quot
Quote:

amidogen said:
Quote:

Unknown error said:
They put carbon in it aparrently to protect the spores from bacteria. They should work fine



:callingbullshit:

How the fuck does carbon protect spores?

It's a trick to make people think they got loaded syringes when they probably got 10 cc's of tap water.

OP, I know the email has been sent, but you have to make sure you make no indications of using them for anything besides microscopy, otherwise they'll hide behind you using them for illegal purposes as invalidating any warranty they offer. If they're pulling this sort of shit though, I imagine they'll pull that card regardless.





Yup,  said I couldn't see them spores in the black dye under microscope... let's see what comes out of it...:stoned:


Edited by mushhiehunter (01/11/17 08:24 AM)


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Re: cyanescens spore syringe black?? [Re: Unknown error]
    #24000484 - 01/11/17 08:28 AM (7 years, 19 days ago)

Quote:

Unknown error said:
Just what they said when I got mine. Had no problems




Did the vendor start with "a" and end with "s" and was based in Netherlands?


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Re: cyanescens spore syringe black?? [Re: mushhiehunter]
    #24000516 - 01/11/17 08:50 AM (7 years, 19 days ago)

It's activated charcoal. Yes it is antibacterial, and it also has been known to germinate old ass spores much easier.

Quote:

Mad Season said:
I'd really recommend trying activated charcoal agar. Spore syringe vendors put a bit of charcoal into the syringes to help germinate spores too. Lots of the time that's what the black specks are in the solution lol

Forced hydration works for old spores in a syringe too. I got a couple PE syringes I couldn't get to germinate until I did the forced hydration tek

I also heard snake venom can help too, but who has that right? lol..



Quote:

RogerRabbit said:
Interesting stuff.  I add activated charcoal to my agar when doing specialty experiments such as germinating very old spores or crossing strains.  I've noticed healthier mycelium when grown on activated charcoal, but not necessarily faster or more growth.

I've also noticed a decrease in bacterial contamination when charcoal is used, and bacterial colonies that do get started, seem to stall out after a couple of days, then are overran by the mycelium.

I doubt it's the small amount of infrared that is suppressing the bacteria, but who knows?



Quote:

cronicr said:
Quote:

stareatclouds said:
I thought the black shit in globs was charcoal added to the syringes to appear better to n00bs?



it's active charcoal and yes some vendors do to help contams and also get away with lc




Though that's a lot of it. Makes you wonder what they're trying to hide.


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Edited by Mad Season (01/11/17 08:58 AM)


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Re: cyanescens spore syringe black?? [Re: Mad Season]
    #24000550 - 01/11/17 09:05 AM (7 years, 19 days ago)

Quote:

Mad Season said:
It's activated charcoal. Yes it is antibacterial, and it also has been known to germinate old ass spores much easier.

Quote:

Mad Season said:
I'd really recommend trying activated charcoal agar. Spore syringe vendors put a bit of charcoal into the syringes to help germinate spores too. Lots of the time that's what the black specks are in the solution lol

Forced hydration works for old spores in a syringe too. I got a couple PE syringes I couldn't get to germinate until I did the forced hydration tek

I also heard snake venom can help too, but who has that right? lol..



Quote:

RogerRabbit said:
Interesting stuff.  I add activated charcoal to my agar when doing specialty experiments such as germinating very old spores or crossing strains.  I've noticed healthier mycelium when grown on activated charcoal, but not necessarily faster or more growth.

I've also noticed a decrease in bacterial contamination when charcoal is used, and bacterial colonies that do get started, seem to stall out after a couple of days, then are overran by the mycelium.

I doubt it's the small amount of infrared that is suppressing the bacteria, but who knows?



Quote:

cronicr said:
Quote:

stareatclouds said:
I thought the black shit in globs was charcoal added to the syringes to appear better to n00bs?



it's active charcoal and yes some vendors do to help contams and also get away with lc




Though that's a lot of it. Makes you wonder what they're trying to hide.




Well I don't know how companies get hold of cyanescens spores... if they grow them indoor and then take a spore-print (cyanescens not being the easiest to cultivate indoors) or if they actually go out... (I don't want to sound dumb for thinking the latter though...)


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Re: cyanescens spore syringe black?? [Re: mushhiehunter]
    #24000560 - 01/11/17 09:08 AM (7 years, 19 days ago)

I can almost guarantee it's from outdoors. For pretty much any exotic, expect it to be from outdoors, which means you will need agar for contam cleanup.


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Re: cyanescens spore syringe black?? [Re: Mad Season] * 1
    #24000562 - 01/11/17 09:10 AM (7 years, 19 days ago)

Quote:

Mad Season said:
I can almost guarantee it's from outdoors.



Well then the anti-bacterial solution makes kinda sense, no??


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Re: cyanescens spore syringe black?? [Re: mushhiehunter]
    #24000571 - 01/11/17 09:12 AM (7 years, 19 days ago)

I've had syringes that are clear with lots of exotics before lol. Why yours is filled to the brim with charcoal is not clear at all, because for all we know, this is just an LC and they're trying to hide that. Regardless you should still start it on agar.


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Re: cyanescens spore syringe black?? [Re: Mad Season]
    #24000579 - 01/11/17 09:15 AM (7 years, 19 days ago)

Quote:

Mad Season said:
I've had syringes that are clear with lots of exotics before lol. Why yours is filled to the brim with charcoal is not clear at all, because for all we know, this is just an LC and they're trying to hide that. Regardless you should still start it on agar.




LC as liquid culture?? wouldn't that count as live mycelium thus making it illegal?


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Re: cyanescens spore syringe black?? [Re: mushhiehunter] * 1
    #24000587 - 01/11/17 09:18 AM (7 years, 19 days ago)

Yep. Doesn't stop some of the vendors.


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Re: cyanescens spore syringe black?? [Re: Mad Season]
    #24000589 - 01/11/17 09:20 AM (7 years, 19 days ago)

Ok, will go to agar soon, I'm curious what will pop up..


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Re: cyanescens spore syringe black?? [Re: mushhiehunter]
    #24002869 - 01/12/17 12:32 AM (7 years, 19 days ago)

Plot thickens guys... the vendor is one of the sponsors of shroomery..


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Re: cyanescens spore syringe black?? [Re: mushhiehunter]
    #24003137 - 01/12/17 04:32 AM (7 years, 18 days ago)

I love a good mushroom mystery :popcorn:


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Re: cyanescens spore syringe black?? [Re: LogicaL Chaos]
    #24003145 - 01/12/17 04:40 AM (7 years, 18 days ago)

Quote:

LogicaL Chaos said:
I love a good mushroom mystery :popcorn:




I inoculated 3 pastyplates with the mysterious black liquid of supposed trustworthy origin.. we'll see soon - maybe even very soon if the carbon is hiding LC..


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Re: cyanescens spore syringe black?? [Re: mushhiehunter]
    #24003256 - 01/12/17 07:02 AM (7 years, 18 days ago)

:takingnotes:


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Re: cyanescens spore syringe black?? [Re: Mad Season]
    #24007254 - 01/13/17 02:01 PM (7 years, 17 days ago)

Quote:

mushhiehunter said:
Quote:

Unknown error said:
Just what they said when I got mine. Had no problems




Did the vendor start with "a" and end with "s" and was based in Netherlands?




I can almost bet this is the place. I have got two syringes from them before. Both where black like this.

My first ever grow was P. azurescens. Used 10cc like this on grains and cakes. Had no contam whatsoever. It was loaded with spores and was growing fast and aggressively. Made 6 pathes in the forest and 2 fruited on 2nd year.

Second syringe was P. ovoid. Nothing ever grew out of that. They have refunded my money after sending them pictures of grains with some dates on them. They know how we are going to use them so if you have a issue then write them. I could have send them just any random picture but because i was satisfied with azures and I have written huge 5 star review no they website and mentioned it in the mails i had a full refund.

A......s.net is solid place guys.

Quote:

Mad Season said:
I've had syringes that are clear with lots of exotics before lol. Why yours is filled to the brim with charcoal is not clear at all, because for all we know, this is just an LC and they're trying to hide that. Regardless you should still start it on agar.




This is not LC. You could see if it was probably on this picture in first post. OK I can't be sure about this but mine wasn't for sure and I'm almost certain that this is the same vendor we are talking about coz it's place of a choice for stuff like this in EU (seeds, etno, capsules you name it, and spores of course :wink: ).

Back to the main question.

I think that you can easily try starting them on grains, If you can work agar then do that too. I don't know how they do it but they are seriously quite clean. Know two other ppl in my country who ordered P.cyans from them and grown them without agar cleaning.

This is my review, its first on they website under P. azurescens syringes. And NO they don't pay me :laugh: if anyone wondered.

"My first mushroom project, and such a great luck. With one 10cc syringe I have inoculated 8 jars of spawn and finally ended up with 5 1x1m 10cm deep patches in my local forest and two huge plant pots full of wood chips in a garden. No contamination of any kind. And I wasn't even that careful. Amazing strain. Checked the patches today and they all are doing very well. Can't wait for the autumn."

And finally my grow log from polish growers site. It's in polish but the pictures are there.

http://www.psilosophy.info/forum/?showtopic=20150113203557

:cheers:


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Re: cyanescens spore syringe black?? [Re: Psynonim] * 1
    #24007264 - 01/13/17 02:03 PM (7 years, 17 days ago)

PS. My avatar is those mighty azures mycelium in action :laugh:


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Re: cyanescens spore syringe black?? [Re: Psynonim]
    #24007846 - 01/13/17 05:26 PM (7 years, 17 days ago)

That's some good optimism :smile:. I too have had many exotics with no contaminations, of course that doesn't mean every syringe will be clean. Personally I always start on agar regardless. It really pisses me off when I waste a whole syringe and most of it cobtaminates, when all I could have done was a drop on agar.

Also yeah, I still feel like it's extremely excessive, the amount of charcoal. Why they did it, I'm not sure. Hiding it isn't something people with nothing to hide do though.


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Re: cyanescens spore syringe black?? [Re: Mad Season]
    #24009400 - 01/14/17 10:19 AM (7 years, 16 days ago)

Quote:

Mad Season said:
That's some good optimism :smile:.




I'm always trying to be optimistic. At the time when I have started all this myco fun staring straight to grains was my only option. Since then I have build a FH with my own hands.

Quote:

mushhiehunter said:
Ok, will go to agar soon, I'm curious what will pop up..




Have somehow skipped this. So they are on agar and we will see soon.


Quote:

Mad Season said:
Also yeah, I still feel like it's extremely excessive, the amount of charcoal. Why they did it, I'm not sure. Hiding it isn't something people with nothing to hide do though.




I will write to them tomorrow and ask for some scientific explanation of this carbon thing.

This is what they write next to P. ovoid syringe.

"Please note: The solution in this spore syringe is black. A little carbon has been added to protect the spores from bacteria."

I remember i was looking for more info on the web about this first time I saw this, and there was nothing. I'm wondering more and more where does this knowledge coming from.


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Re: cyanescens spore syringe black?? [Re: Psynonim]
    #24010239 - 01/14/17 04:29 PM (7 years, 16 days ago)

I've had cyanescens syringe from them, COMPLETELY black - I think they use graphite particles, however there were spores and it produced nice mycelium. No regrets. They are solid and sent me a new syringe, because the first one got lost in transit.


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Re: cyanescens spore syringe black?? [Re: Psynonim]
    #24011077 - 01/14/17 11:23 PM (7 years, 16 days ago)

This information has come from people cleaning shit up with charcoal agar. Also found this when searching up stuff on charcoal agar.

https://www.shroomery.org/forums/showflat.php/Number/15583064
It helps germinate tricky spores as well.

I'm going to try it for myself soon tbh, I have activated charcoal lying around for my distiller and a terribly bacterial PEU syringe, so why not?


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Re: cyanescens spore syringe black?? [Re: Mad Season]
    #24032526 - 01/23/17 05:26 AM (7 years, 7 days ago)

UPDATE



Getting some good looking clean growth on my plates, no signs of contams yet.


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Re: cyanescens spore syringe black?? [Re: mushhiehunter]
    #24032533 - 01/23/17 05:32 AM (7 years, 7 days ago)

One additional question for TCs, If the growth doesn't show any signs of contamination, what is the purpose of transfers? I mean what are the dangers of using agar wedge from the first plate to inoculate rye?


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OfflineTiamo
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Re: cyanescens spore syringe black?? [Re: mushhiehunter]
    #24032541 - 01/23/17 05:39 AM (7 years, 7 days ago)

To isolate the growth and get a more aggressive culture.

Found out my dad bought the same syringe from the same vendor... it looked the same. He is inspired by my mushroom growing but he just gone and went made some honey water, boiled it, put it in some jars in open air, and pushed 2cc per jar of spore solution in them. Used a band-aid as filter. :facepalm:

I've gone and send him Let's Grow Mushrooms. :thumbup:


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Re: cyanescens spore syringe black?? [Re: Tiamo]
    #24032547 - 01/23/17 05:46 AM (7 years, 7 days ago)

Quote:

Tiamo said:
To isolate the growth and get a more aggressive culture.

Found out my dad bought the same syringe from the same vendor... it looked the same. He is inspired by my mushroom growing but he just gone and went made some honey water, boiled it, put it in some jars in open air, and pushed 2cc per jar of spore solution in them. Used a band-aid as filter. :facepalm:

I've gone and send him Let's Grow Mushrooms. :thumbup:





Okay, so I'll wait to see which part of the culture exhibits the highest velocity outward and then transfer outer edge of it, correct?


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Re: cyanescens spore syringe black?? [Re: Tiamo]
    #24032560 - 01/23/17 05:58 AM (7 years, 7 days ago)

Quote:

Tiamo said:
To isolate the growth and get a more aggressive culture.

Found out my dad bought the same syringe from the same vendor... it looked the same. He is inspired by my mushroom growing but he just gone and went made some honey water, boiled it, put it in some jars in open air, and pushed 2cc per jar of spore solution in them. Used a band-aid as filter. :facepalm:

I've gone and send him Let's Grow Mushrooms. :thumbup:




Oh and to your dad's case... I guess you could wait and see what happens and maybe try clean the supposed LC on agar, boiled honeywater should be sterile, bandaid is shit but he still might have gotten away with it  :yolo:


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Re: cyanescens spore syringe black?? [Re: mushhiehunter]
    #24032586 - 01/23/17 06:37 AM (7 years, 7 days ago)

The LCD turned cloudy, slimey and green and black and yellow. Lol.


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Re: cyanescens spore syringe black?? [Re: Tiamo] * 2
    #24032671 - 01/23/17 07:54 AM (7 years, 7 days ago)

Quote:

Tiamo said:
The LCD turned cloudy, slimey and green and black and yellow. Lol.






??


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Re: cyanescens spore syringe black?? [Re: mushhiehunter]
    #24033242 - 01/23/17 01:26 PM (7 years, 7 days ago)

Quote:

mushhiehunter said:
LC as liquid culture?? wouldn't that count as live mycelium thus making it illegal?



illegal in many countries, not all. There is one dodgy seller who has 3 types of syringe, one is black and says

Quote:

*FASTER GERMINATION TIMES
*FASTER COLONIZATION TIMES
*HELPS PREVENT FOREIGN MOLDS AND BACTERIA ON SUBSTRATES AT INOCULATION SITES EVEN BEFORE MYCELIUM BEGINS TO GROW
*HELPS PREVENT BACK CONTAMINATION OF THE SYRINGE BEFORE AND AFTER USE, GREAT FOR STORE-ABILITY
*MYCELIUM TENDS TO BE MORE AGGRESSIVE AND LAST LONGER IN ITS VITALITY - ENCOURAGES HEALTHY GROWTH!
*CULTURES AND SPORES STORED IN "NEW MOON" SOLUTION OR MEDIA ALWAYS OUTLAST ANY OTHER MEDIAS OR SOLUTIONS





This has all the hallmarks of being a selected strain LC disguised by carbon/charcoal. Its a great coincidence to be playing on, the fact many sites will be saying the use of carbon is a legit way to combat bacteria, and that they can have supposed outdoor prints which happen to be clean.


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Re: cyanescens spore syringe black?? [Re: blackout]
    #24033277 - 01/23/17 01:38 PM (7 years, 7 days ago)

How does the charcoal prevent bacteria and mold spores but not the desired mushroom spores from growing?


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Re: cyanescens spore syringe black?? [Re: blackout]
    #24033281 - 01/23/17 01:39 PM (7 years, 7 days ago)

That is sketchy lol. This is from a Dutch vendor though, although I know which vendor it is and question why anyone would buy a cyanescens syringe from them. There is another vendor that ships cyanescens cultures on petri dishes, slants, LC and even spawn for cheaper than that syringe. :lol:


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If you have used a Miraculix Psilocybin QTest, could you please share your results?

Shipping free Ps. natalensis spore prints to any address in The Netherlands, just :pm:

:mushroom2: Mush love :mushroom2:


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Re: cyanescens spore syringe black?? [Re: Tiamo]
    #24033299 - 01/23/17 01:43 PM (7 years, 7 days ago)

Probably because not everyone knows about the seller you're talking about? I've bought cyans syringe from the Dutch seller and it worked so.. why not?


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Re: cyanescens spore syringe black?? [Re: Tiamo]
    #24033305 - 01/23/17 01:45 PM (7 years, 7 days ago)

Just wanted to clear something up

Are we talking about Psilocybe cyanescens or Panaeolus cyanescens?

P. cyanescens could be either.

Psilocybe cyanescens spores ain't even black...


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Re: cyanescens spore syringe black?? [Re: Mr Piggy]
    #24033316 - 01/23/17 01:50 PM (7 years, 7 days ago)

I think we're all talking about Psilocybe (at least I do). But it's possible that the vendor puts charcoal in every syringe so...


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Re: cyanescens spore syringe black?? [Re: Adas] * 1
    #24033344 - 01/23/17 02:01 PM (7 years, 7 days ago)

Ps. cyanescens are the species were talking about. The vendor puts charcoal into their syringes. And it isn't LC. I've seen the syringes myself.


--------------------


If you have used a Miraculix Psilocybin QTest, could you please share your results?

Shipping free Ps. natalensis spore prints to any address in The Netherlands, just :pm:

:mushroom2: Mush love :mushroom2:


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