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mushhiehunter
Trichodermatologist



Registered: 10/11/16
Posts: 837
Loc: Česká republika
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Re: cyanescens spore syringe black?? [Re: Mad Season]
#24000589 - 01/11/17 09:20 AM (7 years, 19 days ago) |
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Ok, will go to agar soon, I'm curious what will pop up..
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mushhiehunter
Trichodermatologist



Registered: 10/11/16
Posts: 837
Loc: Česká republika
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Plot thickens guys... the vendor is one of the sponsors of shroomery..
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
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I love a good mushroom mystery
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mushhiehunter
Trichodermatologist



Registered: 10/11/16
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Quote:
LogicaL Chaos said: I love a good mushroom mystery 
I inoculated 3 pastyplates with the mysterious black liquid of supposed trustworthy origin.. we'll see soon - maybe even very soon if the carbon is hiding LC..
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catnip40
xฬ็



Registered: 03/09/12
Posts: 703
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Psynonim
Stranger

Registered: 01/19/15
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Re: cyanescens spore syringe black?? [Re: Mad Season]
#24007254 - 01/13/17 02:01 PM (7 years, 17 days ago) |
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Quote:
mushhiehunter said:
Quote:
Unknown error said: Just what they said when I got mine. Had no problems
Did the vendor start with "a" and end with "s" and was based in Netherlands?
I can almost bet this is the place. I have got two syringes from them before. Both where black like this.
My first ever grow was P. azurescens. Used 10cc like this on grains and cakes. Had no contam whatsoever. It was loaded with spores and was growing fast and aggressively. Made 6 pathes in the forest and 2 fruited on 2nd year.
Second syringe was P. ovoid. Nothing ever grew out of that. They have refunded my money after sending them pictures of grains with some dates on them. They know how we are going to use them so if you have a issue then write them. I could have send them just any random picture but because i was satisfied with azures and I have written huge 5 star review no they website and mentioned it in the mails i had a full refund.
A......s.net is solid place guys.
Quote:
Mad Season said: I've had syringes that are clear with lots of exotics before lol. Why yours is filled to the brim with charcoal is not clear at all, because for all we know, this is just an LC and they're trying to hide that. Regardless you should still start it on agar.
This is not LC. You could see if it was probably on this picture in first post. OK I can't be sure about this but mine wasn't for sure and I'm almost certain that this is the same vendor we are talking about coz it's place of a choice for stuff like this in EU (seeds, etno, capsules you name it, and spores of course ).
Back to the main question.
I think that you can easily try starting them on grains, If you can work agar then do that too. I don't know how they do it but they are seriously quite clean. Know two other ppl in my country who ordered P.cyans from them and grown them without agar cleaning.
This is my review, its first on they website under P. azurescens syringes. And NO they don't pay me if anyone wondered.
"My first mushroom project, and such a great luck. With one 10cc syringe I have inoculated 8 jars of spawn and finally ended up with 5 1x1m 10cm deep patches in my local forest and two huge plant pots full of wood chips in a garden. No contamination of any kind. And I wasn't even that careful. Amazing strain. Checked the patches today and they all are doing very well. Can't wait for the autumn."
And finally my grow log from polish growers site. It's in polish but the pictures are there.
http://www.psilosophy.info/forum/?showtopic=20150113203557
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Psynonim
Stranger

Registered: 01/19/15
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Re: cyanescens spore syringe black?? [Re: Psynonim] 1
#24007264 - 01/13/17 02:03 PM (7 years, 17 days ago) |
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PS. My avatar is those mighty azures mycelium in action
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: cyanescens spore syringe black?? [Re: Psynonim]
#24007846 - 01/13/17 05:26 PM (7 years, 17 days ago) |
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That's some good optimism . I too have had many exotics with no contaminations, of course that doesn't mean every syringe will be clean. Personally I always start on agar regardless. It really pisses me off when I waste a whole syringe and most of it cobtaminates, when all I could have done was a drop on agar.
Also yeah, I still feel like it's extremely excessive, the amount of charcoal. Why they did it, I'm not sure. Hiding it isn't something people with nothing to hide do though.
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Psynonim
Stranger

Registered: 01/19/15
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Re: cyanescens spore syringe black?? [Re: Mad Season]
#24009400 - 01/14/17 10:19 AM (7 years, 16 days ago) |
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Quote:
Mad Season said: That's some good optimism .
I'm always trying to be optimistic. At the time when I have started all this myco fun staring straight to grains was my only option. Since then I have build a FH with my own hands.
Quote:
mushhiehunter said: Ok, will go to agar soon, I'm curious what will pop up..
Have somehow skipped this. So they are on agar and we will see soon.
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Mad Season said: Also yeah, I still feel like it's extremely excessive, the amount of charcoal. Why they did it, I'm not sure. Hiding it isn't something people with nothing to hide do though.
I will write to them tomorrow and ask for some scientific explanation of this carbon thing.
This is what they write next to P. ovoid syringe.
"Please note: The solution in this spore syringe is black. A little carbon has been added to protect the spores from bacteria."
I remember i was looking for more info on the web about this first time I saw this, and there was nothing. I'm wondering more and more where does this knowledge coming from.
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Adas
Lonely Dreamer



Registered: 12/22/16
Posts: 5,270
Loc: Central EU
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Re: cyanescens spore syringe black?? [Re: Psynonim]
#24010239 - 01/14/17 04:29 PM (7 years, 16 days ago) |
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I've had cyanescens syringe from them, COMPLETELY black - I think they use graphite particles, however there were spores and it produced nice mycelium. No regrets. They are solid and sent me a new syringe, because the first one got lost in transit.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: cyanescens spore syringe black?? [Re: Psynonim]
#24011077 - 01/14/17 11:23 PM (7 years, 15 days ago) |
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This information has come from people cleaning shit up with charcoal agar. Also found this when searching up stuff on charcoal agar.
https://www.shroomery.org/forums/showflat.php/Number/15583064 It helps germinate tricky spores as well.
I'm going to try it for myself soon tbh, I have activated charcoal lying around for my distiller and a terribly bacterial PEU syringe, so why not?
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mushhiehunter
Trichodermatologist



Registered: 10/11/16
Posts: 837
Loc: Česká republika
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Re: cyanescens spore syringe black?? [Re: Mad Season]
#24032526 - 01/23/17 05:26 AM (7 years, 7 days ago) |
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UPDATE

Getting some good looking clean growth on my plates, no signs of contams yet.
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mushhiehunter
Trichodermatologist



Registered: 10/11/16
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One additional question for TCs, If the growth doesn't show any signs of contamination, what is the purpose of transfers? I mean what are the dangers of using agar wedge from the first plate to inoculate rye?
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Tiamo
Trust in LITFA



Registered: 04/07/16
Posts: 1,935
Loc: Amsterdam
Last seen: 4 hours, 27 minutes
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To isolate the growth and get a more aggressive culture.
Found out my dad bought the same syringe from the same vendor... it looked the same. He is inspired by my mushroom growing but he just gone and went made some honey water, boiled it, put it in some jars in open air, and pushed 2cc per jar of spore solution in them. Used a band-aid as filter. 
I've gone and send him Let's Grow Mushrooms.
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If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
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mushhiehunter
Trichodermatologist



Registered: 10/11/16
Posts: 837
Loc: Česká republika
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Re: cyanescens spore syringe black?? [Re: Tiamo]
#24032547 - 01/23/17 05:46 AM (7 years, 7 days ago) |
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Quote:
Tiamo said: To isolate the growth and get a more aggressive culture.
Found out my dad bought the same syringe from the same vendor... it looked the same. He is inspired by my mushroom growing but he just gone and went made some honey water, boiled it, put it in some jars in open air, and pushed 2cc per jar of spore solution in them. Used a band-aid as filter. 
I've gone and send him Let's Grow Mushrooms. 
Okay, so I'll wait to see which part of the culture exhibits the highest velocity outward and then transfer outer edge of it, correct?
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mushhiehunter
Trichodermatologist



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Re: cyanescens spore syringe black?? [Re: Tiamo]
#24032560 - 01/23/17 05:58 AM (7 years, 7 days ago) |
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Quote:
Tiamo said: To isolate the growth and get a more aggressive culture.
Found out my dad bought the same syringe from the same vendor... it looked the same. He is inspired by my mushroom growing but he just gone and went made some honey water, boiled it, put it in some jars in open air, and pushed 2cc per jar of spore solution in them. Used a band-aid as filter. 
I've gone and send him Let's Grow Mushrooms. 
Oh and to your dad's case... I guess you could wait and see what happens and maybe try clean the supposed LC on agar, boiled honeywater should be sterile, bandaid is shit but he still might have gotten away with it
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Tiamo
Trust in LITFA



Registered: 04/07/16
Posts: 1,935
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The LCD turned cloudy, slimey and green and black and yellow. Lol.
--------------------
If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
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mushhiehunter
Trichodermatologist



Registered: 10/11/16
Posts: 837
Loc: Česká republika
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Re: cyanescens spore syringe black?? [Re: Tiamo] 2
#24032671 - 01/23/17 07:54 AM (7 years, 7 days ago) |
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Quote:
Tiamo said: The LCD turned cloudy, slimey and green and black and yellow. Lol.
??
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blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 2 months, 25 days
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Quote:
mushhiehunter said: LC as liquid culture?? wouldn't that count as live mycelium thus making it illegal?
illegal in many countries, not all. There is one dodgy seller who has 3 types of syringe, one is black and says
Quote:
*FASTER GERMINATION TIMES *FASTER COLONIZATION TIMES *HELPS PREVENT FOREIGN MOLDS AND BACTERIA ON SUBSTRATES AT INOCULATION SITES EVEN BEFORE MYCELIUM BEGINS TO GROW *HELPS PREVENT BACK CONTAMINATION OF THE SYRINGE BEFORE AND AFTER USE, GREAT FOR STORE-ABILITY *MYCELIUM TENDS TO BE MORE AGGRESSIVE AND LAST LONGER IN ITS VITALITY - ENCOURAGES HEALTHY GROWTH! *CULTURES AND SPORES STORED IN "NEW MOON" SOLUTION OR MEDIA ALWAYS OUTLAST ANY OTHER MEDIAS OR SOLUTIONS
This has all the hallmarks of being a selected strain LC disguised by carbon/charcoal. Its a great coincidence to be playing on, the fact many sites will be saying the use of carbon is a legit way to combat bacteria, and that they can have supposed outdoor prints which happen to be clean.
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Adas
Lonely Dreamer



Registered: 12/22/16
Posts: 5,270
Loc: Central EU
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Re: cyanescens spore syringe black?? [Re: blackout]
#24033277 - 01/23/17 01:38 PM (7 years, 7 days ago) |
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How does the charcoal prevent bacteria and mold spores but not the desired mushroom spores from growing?
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