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Offlinemushhiehunter
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cyanescens spore syringe black??
    #24000190 - 01/11/17 04:02 AM (7 years, 19 days ago)

Hey guys so I bought a spore syringe with p. cyanescens spores from a EU vendor and to my surprise it has dark liquid in it. Now I suspect it being a dye since the amount of spores needed for such a discoloration would be massive... What do you guys think??


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OfflineTiamo
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Re: cyanescens spore syringe black?? [Re: mushhiehunter]
    #24000194 - 01/11/17 04:06 AM (7 years, 19 days ago)

Yea, that looks like someone dyed the water in it. :facepalm: I hate when vendors do this.


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Offlinemushhiehunter
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Re: cyanescens spore syringe black?? [Re: Tiamo]
    #24000199 - 01/11/17 04:10 AM (7 years, 19 days ago)

Quote:

Tiamo said:
Yea, that looks like someone dyed the water in it. :facepalm: I hate when vendors do this.




Well maybe your average Joe feels better when he sees he's not buying clear water... just imagine the number of spores needed for this effect though... it would be possibly more than all humans that ever lived


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Invisibleamidogen
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Re: cyanescens spore syringe black?? [Re: mushhiehunter]
    #24000306 - 01/11/17 06:07 AM (7 years, 19 days ago)

.


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Edited by amidogen (04/19/18 01:14 PM)


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Offlinemushhiehunter
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Re: cyanescens spore syringe black?? [Re: amidogen]
    #24000325 - 01/11/17 06:22 AM (7 years, 19 days ago)

Quote:

amidogen said:
Quote:

mushhiehunter said:
Quote:

Tiamo said:
Yea, that looks like someone dyed the water in it. :facepalm: I hate when vendors do this.




Well maybe your average Joe feels better when he sees he's not buying clear water... just imagine the number of spores needed for this effect though... it would be possibly more than all humans that ever lived



:facepalm:

That's the point...they dye the water with coloring so you can't see how few spores they gave you. If I got a syringe like this, I'd assume it's nearly empty or completely empty of viable spores. A reputable vendor with quality syringes will give you one without dye and you'll see the large black clumps of spores floating in the water. I'd ask for a refund. The spores are for microscopy right? How the fuck are you supposed to see black spores suspended in black water? Maybe you'll have luck with it but my bet is it's not a viable syringe. Sorry man.




I know that the discoloration doesn't come from the spores, I was just thinking out lout how many actual spores it would take to get to this effect :laugh:


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Offlinesynthetik
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Re: cyanescens spore syringe black?? [Re: mushhiehunter]
    #24000340 - 01/11/17 06:41 AM (7 years, 19 days ago)

yeah that seems like bullshit... have ya shot a drop on a plate yet?


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Offlinemushhiehunter
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Re: cyanescens spore syringe black?? [Re: synthetik]
    #24000342 - 01/11/17 06:42 AM (7 years, 19 days ago)

Quote:

synthetik said:
yeah that seems like bullshit... have ya shot a drop on a plate yet?




Nope, but I sent them a heated e-mail this morning.. we'll see what comes out of it....


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Re: cyanescens spore syringe black?? [Re: mushhiehunter]
    #24000379 - 01/11/17 07:07 AM (7 years, 19 days ago)

They put carbon in it aparrently to protect the spores from bacteria. They should work fine


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Re: cyanescens spore syringe black?? [Re: Unknown error]
    #24000408 - 01/11/17 07:35 AM (7 years, 19 days ago)

They don't do it to protect the spores, they do it so their product looks better.


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Shipping free Ps. natalensis spore prints to any address in The Netherlands, just :pm:

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Invisibleamidogen
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Re: cyanescens spore syringe black?? [Re: Unknown error]
    #24000411 - 01/11/17 07:40 AM (7 years, 19 days ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 01:14 PM)


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Re: cyanescens spore syringe black?? [Re: Tiamo]
    #24000418 - 01/11/17 07:45 AM (7 years, 19 days ago)

Just what they said when I got mine. Had no problems


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Offlinemushhiehunter
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Re: cyanescens spore syringe black?? [Re: amidogen]
    #24000478 - 01/11/17 08:24 AM (7 years, 19 days ago)

Quote:

Unknown error said:
They put carbon in it aparrently to protect the spores from bacteria. They should work fine[/quot
Quote:

amidogen said:
Quote:

Unknown error said:
They put carbon in it aparrently to protect the spores from bacteria. They should work fine



:callingbullshit:

How the fuck does carbon protect spores?

It's a trick to make people think they got loaded syringes when they probably got 10 cc's of tap water.

OP, I know the email has been sent, but you have to make sure you make no indications of using them for anything besides microscopy, otherwise they'll hide behind you using them for illegal purposes as invalidating any warranty they offer. If they're pulling this sort of shit though, I imagine they'll pull that card regardless.





Yup,  said I couldn't see them spores in the black dye under microscope... let's see what comes out of it...:stoned:


Edited by mushhiehunter (01/11/17 08:24 AM)


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Offlinemushhiehunter
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Re: cyanescens spore syringe black?? [Re: Unknown error]
    #24000484 - 01/11/17 08:28 AM (7 years, 19 days ago)

Quote:

Unknown error said:
Just what they said when I got mine. Had no problems




Did the vendor start with "a" and end with "s" and was based in Netherlands?


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InvisibleMad Season
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Re: cyanescens spore syringe black?? [Re: mushhiehunter]
    #24000516 - 01/11/17 08:50 AM (7 years, 19 days ago)

It's activated charcoal. Yes it is antibacterial, and it also has been known to germinate old ass spores much easier.

Quote:

Mad Season said:
I'd really recommend trying activated charcoal agar. Spore syringe vendors put a bit of charcoal into the syringes to help germinate spores too. Lots of the time that's what the black specks are in the solution lol

Forced hydration works for old spores in a syringe too. I got a couple PE syringes I couldn't get to germinate until I did the forced hydration tek

I also heard snake venom can help too, but who has that right? lol..



Quote:

RogerRabbit said:
Interesting stuff.  I add activated charcoal to my agar when doing specialty experiments such as germinating very old spores or crossing strains.  I've noticed healthier mycelium when grown on activated charcoal, but not necessarily faster or more growth.

I've also noticed a decrease in bacterial contamination when charcoal is used, and bacterial colonies that do get started, seem to stall out after a couple of days, then are overran by the mycelium.

I doubt it's the small amount of infrared that is suppressing the bacteria, but who knows?



Quote:

cronicr said:
Quote:

stareatclouds said:
I thought the black shit in globs was charcoal added to the syringes to appear better to n00bs?



it's active charcoal and yes some vendors do to help contams and also get away with lc




Though that's a lot of it. Makes you wonder what they're trying to hide.


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Edited by Mad Season (01/11/17 08:58 AM)


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Offlinemushhiehunter
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Re: cyanescens spore syringe black?? [Re: Mad Season]
    #24000550 - 01/11/17 09:05 AM (7 years, 19 days ago)

Quote:

Mad Season said:
It's activated charcoal. Yes it is antibacterial, and it also has been known to germinate old ass spores much easier.

Quote:

Mad Season said:
I'd really recommend trying activated charcoal agar. Spore syringe vendors put a bit of charcoal into the syringes to help germinate spores too. Lots of the time that's what the black specks are in the solution lol

Forced hydration works for old spores in a syringe too. I got a couple PE syringes I couldn't get to germinate until I did the forced hydration tek

I also heard snake venom can help too, but who has that right? lol..



Quote:

RogerRabbit said:
Interesting stuff.  I add activated charcoal to my agar when doing specialty experiments such as germinating very old spores or crossing strains.  I've noticed healthier mycelium when grown on activated charcoal, but not necessarily faster or more growth.

I've also noticed a decrease in bacterial contamination when charcoal is used, and bacterial colonies that do get started, seem to stall out after a couple of days, then are overran by the mycelium.

I doubt it's the small amount of infrared that is suppressing the bacteria, but who knows?



Quote:

cronicr said:
Quote:

stareatclouds said:
I thought the black shit in globs was charcoal added to the syringes to appear better to n00bs?



it's active charcoal and yes some vendors do to help contams and also get away with lc




Though that's a lot of it. Makes you wonder what they're trying to hide.




Well I don't know how companies get hold of cyanescens spores... if they grow them indoor and then take a spore-print (cyanescens not being the easiest to cultivate indoors) or if they actually go out... (I don't want to sound dumb for thinking the latter though...)


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InvisibleMad Season
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Re: cyanescens spore syringe black?? [Re: mushhiehunter]
    #24000560 - 01/11/17 09:08 AM (7 years, 19 days ago)

I can almost guarantee it's from outdoors. For pretty much any exotic, expect it to be from outdoors, which means you will need agar for contam cleanup.


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Offlinemushhiehunter
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Re: cyanescens spore syringe black?? [Re: Mad Season] * 1
    #24000562 - 01/11/17 09:10 AM (7 years, 19 days ago)

Quote:

Mad Season said:
I can almost guarantee it's from outdoors.



Well then the anti-bacterial solution makes kinda sense, no??


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InvisibleMad Season
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Re: cyanescens spore syringe black?? [Re: mushhiehunter]
    #24000571 - 01/11/17 09:12 AM (7 years, 19 days ago)

I've had syringes that are clear with lots of exotics before lol. Why yours is filled to the brim with charcoal is not clear at all, because for all we know, this is just an LC and they're trying to hide that. Regardless you should still start it on agar.


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How to shroomery like a pro! (Seriously, everyone read this!)
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No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Offlinemushhiehunter
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Re: cyanescens spore syringe black?? [Re: Mad Season]
    #24000579 - 01/11/17 09:15 AM (7 years, 19 days ago)

Quote:

Mad Season said:
I've had syringes that are clear with lots of exotics before lol. Why yours is filled to the brim with charcoal is not clear at all, because for all we know, this is just an LC and they're trying to hide that. Regardless you should still start it on agar.




LC as liquid culture?? wouldn't that count as live mycelium thus making it illegal?


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InvisibleMad Season
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Re: cyanescens spore syringe black?? [Re: mushhiehunter] * 1
    #24000587 - 01/11/17 09:18 AM (7 years, 19 days ago)

Yep. Doesn't stop some of the vendors.


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How to shroomery like a pro! (Seriously, everyone read this!)
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Links upon links of literally EVERYTHING UP TO DATE

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