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Offlineclan91
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Re: Proper Surface Moisture / When to Increase FAE [Re: Caps McGee]
    #25306748 - 07/03/18 04:21 PM (5 years, 6 months ago)

:thumbup:


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OfflineEnkidu
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Re: Proper Surface Moisture / When to Increase FAE [Re: clan91]
    #25307277 - 07/03/18 10:16 PM (5 years, 6 months ago)

Yeah I doubt I would be spawning a jar if it didn't Colonize fully and had a wet spot that wouldn't colonize. Hope for the best I suppose.

How did you prep snd innocukste the grain ?

G2g is cool, will colonize super fast.

Temp droping at night is fine and found in nature, ime seems temp really mainly effects how fast shit will colonize and fruit and seems better at around 70-75 from my experience but maybe different for others and should only effect rate of grow and not quality. Some say cooler fruiting makes stronger cubes but ime it takes longer than I'm willing to wait


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Within You , Without You


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Offlineclan91
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Re: Proper Surface Moisture / When to Increase FAE [Re: Enkidu]
    #25307990 - 07/04/18 10:33 AM (5 years, 6 months ago)

Quote:

Enkidu said:
Yeah I doubt I would be spawning a jar if it didn't Colonize fully and had a wet spot that wouldn't colonize. Hope for the best I suppose.

How did you prep snd innocukste the grain ?

G2g is cool, will colonize super fast.

Temp droping at night is fine and found in nature, ime seems temp really mainly effects how fast shit will colonize and fruit and seems better at around 70-75 from my experience but maybe different for others and should only effect rate of grow and not quality. Some say cooler fruiting makes stronger cubes but ime it takes longer than I'm willing to wait




Yeah, in retrospect, I probably should have left that jar out of the mix. All of the others consolidated completely and all smelled strongly of shrooms, didn't look weird, etc.

I inoculated agar with MS syringe (Ecuadorian), did one agar transfer, then inoculated oat jars with agar sections from that (all in a SAB). Agar plates just recently developed what looks like contamination, several weeks after transfers to jars and sitting out without cling wrap the whole time. Once the jars were fully colonized, I spawned to coir substrate with an ~6 quart jars:1 650g coir brick ratio (3:.5 for the smaller 34qt tub).

One possible issue though is that I mixed 1.5 bricks coir in 5 gallon tub (following BODs TEK), which made it tricky to mix the coir properly and to gauge wetness. As a result, I wound up adding too much water and had to add additional dry coir to the mix to get to field capacity (I didn't have vermiculite on hand and was leaving on a trip so I just did my best with the coir I had left over).

This might have messed with my moisture or possibly the spawn:coir ratio, because I just had to guess on how much coir to put in each tub. So, it's possible that my actual spawn:coir ratio was <6:1, which is probably not ideal for quick consolidation.

On the bright side, at Day 12 and I still haven't seen any green fuzz or other obvious contamination. Still crossing fingers and hoping for the best. It's been 20+ years since I've eaten shrooms, so I think I can wait a bit longer :wink:


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InvisibleCaps McGee
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Re: Proper Surface Moisture / When to Increase FAE [Re: clan91]
    #25308094 - 07/04/18 11:43 AM (5 years, 6 months ago)

Not wrapping your plates will cause contamination... also, if you're not working with spores, (clones,transfers, or g2a)you can mix hydrogen peroxide  (3% solution at 2%, or 6% solution at 1% of your agar recipe) and this will stop contaminant spores from germinating...


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Offlinepixelpopper
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Re: Proper Surface Moisture / When to Increase FAE [Re: Caps McGee]
    #25308158 - 07/04/18 12:10 PM (5 years, 6 months ago)

Quote:

Caps McGee said:
Not wrapping your plates will cause contamination...





Are you talking about regular lab style dishes? I don't use those but pasty plates definitely do not require wrapping


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InvisibleCaps McGee
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Re: Proper Surface Moisture / When to Increase FAE [Re: pixelpopper]
    #25308170 - 07/04/18 12:17 PM (5 years, 6 months ago)

I've only used the plastic petri dishes...  looked for containers today to do some no pour pasty's... I actually poured 3 extra dishes for different control groups... one was wrapped, and kept at room temp: no contams, one was not wrapped, and kept at room temp: cobweb, trichoderma, and bacterial I believe... the 3rd was left open in my SAB: no contaminants


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Offlineclan91
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Re: Proper Surface Moisture / When to Increase FAE [Re: Caps McGee]
    #25308175 - 07/04/18 12:23 PM (5 years, 6 months ago)

Quote:

Caps McGee said:
Not wrapping your plates will cause contamination... also, if you're not working with spores, (clones,transfers, or g2a)you can mix hydrogen peroxide  (3% solution at 2%, or 6% solution at 1% of your agar recipe) and this will stop contaminant spores from germinating...




Plates were wrapped up until the point I transferred wedges to my grain jars. After that, I left them unwrapped in the SAB (and eventually just moved them to a small plastic tub) just to see what would happen to them. As I mentioned, they looked totally contam free up until a few days ago, when I took a look at them and noticed some little white specs here and there in the agar. Again, this was after ~3 wks of sitting unwrapped in a plastic bin. I only point this out to highlight that the plates were clean when I made the a2g transfer.

Also, for my first agar transfer following germination, I did in fact add hydrogen peroxide to my recipe. Plates looked completely contaminant free when a2g was done, and jars all looked and smelled fine when spawn to bulk was done, aside from one that had an area that did not fully consolidate, which I attributed to insufficient shaking, but that may have been bacterial. That jar smelled just like the rest though, no weird colors, growths, etc., so who knows?


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InvisibleCaps McGee
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Re: Proper Surface Moisture / When to Increase FAE [Re: clan91]
    #25308182 - 07/04/18 12:26 PM (5 years, 6 months ago)

I understood, was just concurring... this is a great hobby... I feel like a scientist... Nay, I AM a scientist! Lol


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Offlineclan91
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Re: Proper Surface Moisture / When to Increase FAE [Re: Caps McGee]
    #25308185 - 07/04/18 12:27 PM (5 years, 6 months ago)

Ah, cool. Sorry for the ridiculously long reply, in that case. :thumbup:


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InvisibleCaps McGee
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Re: Proper Surface Moisture / When to Increase FAE [Re: clan91]
    #25308197 - 07/04/18 12:32 PM (5 years, 6 months ago)

Quote:

clan91 said:
Ah, cool. Sorry for the ridiculously long reply, in that case. :thumbup:



:rofl:
It's all good... I post a lot, just spreading quasi-relevant information I've gained here... trying to give back lol, I'm sure a lot of folks are like"duh!" But I ran into a lot of spots where the answer to the question I was asking,  only lead to other questions..
Idk, is cool though, the whole process


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Offlineclan91
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Re: Proper Surface Moisture / When to Increase FAE [Re: Caps McGee]
    #25308229 - 07/04/18 12:45 PM (5 years, 6 months ago)

Yeah, happy to get any input I can get. Not sure I'm helping anyone else out at this point, but hopefully some day. :mushroom2:


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InvisibleCaps McGee
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Re: Proper Surface Moisture / When to Increase FAE [Re: clan91]
    #25308232 - 07/04/18 12:46 PM (5 years, 6 months ago)

I'm trying myself man... we'll get there!


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OfflineEnkidu
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Re: Proper Surface Moisture / When to Increase FAE [Re: Caps McGee]
    #25308689 - 07/04/18 06:06 PM (5 years, 6 months ago)

Everything you did sound fine to me really :shrug:

You spawned the 6 to a monotub?

You can add verm to the coir mix if it's too wet, I do it like Everytime. Just get field capacity and you're good, spawn with clean jars and should be good


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Offlineclan91
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Re: Proper Surface Moisture / When to Increase FAE [Re: Enkidu]
    #25309862 - 07/05/18 01:04 PM (5 years, 6 months ago)

Quote:

Enkidu said:
Everything you did sound fine to me really :shrug:

You spawned the 6 to a monotub?

You can add verm to the coir mix if it's too wet, I do it like Everytime. Just get field capacity and you're good, spawn with clean jars and should be good




Yeah, 6 qt to 54 qt monotub and 3 qts to 34 qt tub (had 10 jars and kept one out for g2g).

It's still going. No green fuzz yet but growth is still slow. Here's a shot of the surface of the larger tub from today, to bring this back on thread:



There's some pooling from water dripping on the surface from the sides. The middle looks dry. The top left in particular looks a little strange as well. Do those little knot things forming suggest bacterial contamination?

I also worried that some of what I think is mycelium might actually be cobweb mold, but I don't think so. Smells like mushrooms when the lid is open. Not musty, chemically, coconutty, or other smells associated with various forms of contamination.

One possibly relevant detail: when I originally inoculated my agar with a MS syringe, there was zero growth over the course of about a week when the plates were in my basement (where the tubs are now and ambient temps are 67-71 F). Once I moved them upstairs (where the temps were 75-82 F, mycelium popped up immediately and grew pretty rapidly.

I assumed a bit lower temps for spawning to bulk would be advisable, due to heat generated from the mycelium itself + the lights. Perhaps I'm wrong here? Do different varieties or lineages, or whatever you want to call them, prefer different conditions? Perhaps the Ecuadorians I'm working with like it a bit warmer? The grain jars were also stored upstairs (75-82 F) during consolidation.

Thanks for the input!


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Offlineclan91
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Re: Proper Surface Moisture / When to Increase FAE [Re: clan91]
    #25309941 - 07/05/18 01:40 PM (5 years, 6 months ago)

Here are a few close-ups of different parts of the tub shown in the last post:

top left


middle


bottom left


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InvisibleCaps McGee
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Re: Proper Surface Moisture / When to Increase FAE [Re: clan91]
    #25310413 - 07/05/18 06:09 PM (5 years, 6 months ago)

Are you misting a lot? Looks a little matted  (mine's bad, let it dry out and now I'm stuck misting: 4th flush though lol) other than that, actually looks pretty good! And those are hyphal knots, fruits be coming!


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InvisibleCaps McGee
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Re: Proper Surface Moisture / When to Increase FAE [Re: Caps McGee]
    #25310424 - 07/05/18 06:19 PM (5 years, 6 months ago)

It's typical for ms inoculation to take anywhere from 2 days to a full month to start showing signs of growth: depending on variety, age and storage of print/ syringe... it's much slower than using an innoculant such as agar or LC, due to germination period for spores to "get together"... Also, higher temps will generate faster growth, but is more favorable for contaminants, and I've read (may be false, totally subjective) that above 85-90 effects potency... I think 74-78 is ideal, but as cool as 65 is ok... myc growth will slow, but the lower temp gives it advantage over competitor molds and bacteria... hope this helps


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Offlineclan91
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Re: Proper Surface Moisture / When to Increase FAE [Re: Caps McGee]
    #25310427 - 07/05/18 06:22 PM (5 years, 6 months ago)

Quote:

Caps McGee said:
Are you misting a lot? Looks a little matted  (mine's bad, let it dry out and now I'm stuck misting: 4th flush though lol) other than that, actually looks pretty good! And those are hyphal knots, fruits be coming!




Ooohh, just what I wanted to hear! :thumbup:

I've had the lid flipped upside down so I have been misting once or twice a day. Mostly because the middle area looks so dry. I've gone back and forth thinking it's too wet then too dry and back again. It's a rollercoaster of emotions! :crazy2:

Thanks for the input!


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Offlineclan91
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Re: Proper Surface Moisture / When to Increase FAE [Re: Caps McGee]
    #25310428 - 07/05/18 06:23 PM (5 years, 6 months ago)

Quote:

Caps McGee said:
It's typical for ms inoculation to take anywhere from 2 days to a full month to start showing signs of growth: depending on variety, age and storage of print/ syringe... it's much slower than using an innoculant such as agar or LC, due to germination period for spores to "get together"... Also, higher temps will generate faster growth, but is more favorable for contaminants, and I've read (may be false, totally subjective) that above 85-90 effects potency... I think 74-78 is ideal, but as cool as 65 is ok... myc growth will slow, but the lower temp gives it advantage over competitor molds and bacteria... hope this helps




I suppose it could have been a coincidence that myc appeared after moving upstairs. who knows?

I guess I'll leave things where they are for now then (although I'm tempted to move one of my tubs to a warmer area just to see what happens)


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InvisibleCaps McGee
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Re: Proper Surface Moisture / When to Increase FAE [Re: clan91]
    #25310438 - 07/05/18 06:31 PM (5 years, 6 months ago)

Do it!


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