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OfflineDactylium
Don't touch me I'm sterile


Registered: 08/12/16
Posts: 696
Last seen: 10 months, 13 days
Bottle grow I gone and done * 1
    #23997059 - 01/10/17 12:24 AM (7 years, 20 days ago)

INTRO

I've been hearing a lot about bottle grows recently and I thought I'd do my best imitation. I saw Muda's bottle tek which is great and rather extensive. He goes into all these different recipes and there's a lot of steps to prep everything just right. Problem is I'm a lazy fuck, so I did my own half-assed take on his tek. With 12 empty ziplocs and a heart of mold, I began my journey.


PHASE ONE: CULTURE

And they say white people don't have culture. Ummmm, try again sweetie:

Here I've got an Albino Penis Envy culture and a Chitwan culture. Both had clean growth from the syringe so I only took a transfer or two of each and called it a day.


PHASE TWO: SUBSTRATE

As I previously stated, I'm lazy as hell. So instead of picking one of Muda's recipes I stuck to what I already know from growing in monos. I used Inocuole's Impossibly Simple Whole Oat Prep.

Except I skipped the part where he pours boiling water over the oats a second time to help them steam dry. I wasn't at all concerned about any excess water. "Why would you do such a thing?"  I'm glad you asked. I used the bucket tek:

And no, it's not 50/50 coir verm god damn it. His user name is Damion5050. For god's sake the tek is titled "My Elementary Coir Tek". Anyway, you might also be asking yourself why I opted for fine verm over coarse. The answer is simple: I didn't have any. The store is like 3 miles away and it snowed down here. I've been doing nothing but eating milk and egg sandwiches for days. Where was I going with this again? Oh, right. From what I understand the bucket tek runs a little on the dry side so I figured any excess water would easily be absorbed by the coir/verm without going over field capacity.


PHASE THREE

I had to load the bottles up. I ended up doing 1.5 cups oats to 2 cups coir/verm. I was originally going to do 1 cup of oats but I had some extra room.

I loaded them into the pressure cooker FOUR AT A TIME because that's all I could fit. I was pissed, that shit took like 9 or 10 hours. I did 2 hour cycles at 15psi and let the pressure cooker reach 0psi before loading the next batch.


PHASE FOUR: INOCULATION

Now that we're done with all that tedious bullshit, it's time to get into the nitty gritty. This is where all my hard work can be ruined with the wave of a hand. I used Munchauzen's Blenderless Liquid Inoculant. I had issues with the jars forming a vacuum last time I used his tek because I sterilized my water jars with my grain jars. The 20 minute PC cycle made things easier to open, sort of.

HOWEVER, I got too clumsy opening second water jar for my Chitwan culture and ended up touching the underside of the lid when the seal let go. FUCK!
I've got a flight early in the morning so I couldn't afford to sterilize it again. It was time for hard mode:

First I unscrewed the lids of the remaining 6 bottles and gently lifted them so they'd be ready to be removed easily with one hand. I also popped lid off of my pasty plate and gently set it back down.

Then I flame sterilized the SHIT out of my scalpel and slid the lid off to the side of the plate. I held the plate at a 45 degree angle so as to avoid my hand being over the media.

I proceeded to cut a grid into my agar to divide it into rectangular chunks. I speared each chunk with my scalpel, gently slid the lid of the bottle of to the side, and used the inside of the bottle to slide the agar chunk off of the blade. I figure it was better to let my agar touch the inside of the bottle than wave the scalpel around like a madman trying to get it off. I'll take a video next time so you guys can tell me what a fuckin' doofus I am.


PHASE FOUR AND A HALF: COLONIZATION



We're nearing the final phase, boys. I shook the shit out of the bottles and gave them a little love tap to pack 'em down a little. Then I loosened the lids slightly to allow some gas exchange. They'll be sitting near a lamp with a 6500k CFL I've got on 12/12 timing while I'm out of town. I've got a flight to catch in a few hours here and I've been typing this up instead of sleeping. I'll be back in a week with an update on both this grow and my AA+ "Let's All Grow Mushrooms 2017" grow.

PHASE FIVE: FRUITING

Five of the APE bottles finished colonizing pretty quickly so I went ahead and opened em up. I cased them with about 1/4" to 1/2" of fine verm and sprayed 'em down.

Upon opening one of them I saw a grain that wasn't fully enveloped with mycelium so I dug the chunk it was connected to out just to be certain no uncolonized grains were exposed. I ordered some LEDs for the lid of this tote but they're coming from China so it looks like I'm jerry rigging it with a 6500K CFL in this lamp.

It says between 45-50% RH in here and it's set up by my computer so I just look over and mist as necessary, popping the lid on when I leave or go to bed. The other bottles look to be about 2 days off from full colonization. Looking forward to having them join their brethren.

UPDATE:
 


Side pins, so many side pins.


Edited by Dactylium (02/04/17 03:13 PM)


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InvisibleIntelligentxfruit
Earth Hippy


Registered: 01/06/13
Posts: 1,545
Re: Bottle grow I gone and done [Re: Dactylium]
    #23997070 - 01/10/17 12:29 AM (7 years, 20 days ago)

Yeah as long as you weren't rubbing the top edge of your jar you'll be golden dude:thumbup:

always found it super annoying years ago when I used the bucket tek people calling it 50/50 or damions coir tek then I go to search it and can't fucking find it because its called Elementary coir tek

:popcorn:


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Offlinewtfcrazymofo
foil hater
Other


Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
Last seen: 10 hours, 47 minutes
Re: Bottle grow I gone and done [Re: Intelligentxfruit]
    #23997258 - 01/10/17 03:33 AM (7 years, 20 days ago)

To bad about the lack of li.


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




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OfflineTiamo
Trust in LITFA
Male User Gallery


Folding@home Statistics
Registered: 04/07/16
Posts: 1,935
Loc: Amsterdam Flag
Last seen: 6 hours, 23 minutes
Re: Bottle grow I gone and done [Re: Dactylium]
    #23997260 - 01/10/17 03:34 AM (7 years, 20 days ago)

Quote:

Dactylium said:
And they say white people don't have culture. Ummmm, try again sweetie:





I'm starting to think you really are iDubbbz. :rofl:


--------------------


If you have used a Miraculix Psilocybin QTest, could you please share your results?

Shipping free Ps. natalensis spore prints to any address in The Netherlands, just :pm:

:mushroom2: Mush love :mushroom2:


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Invisibleberlinmyc
I'm a teapot

Registered: 12/21/15
Posts: 98
Re: Bottle grow I gone and done [Re: Tiamo]
    #23997266 - 01/10/17 03:39 AM (7 years, 20 days ago)

And it's looks like the culture you have is snow white!


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Offlinesynthetik
biological entity

Registered: 12/15/16
Posts: 194
Last seen: 3 years, 10 months
Re: Bottle grow I gone and done [Re: berlinmyc]
    #23997298 - 01/10/17 04:23 AM (7 years, 20 days ago)

nice following this for sure!


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OfflineDactylium
Don't touch me I'm sterile


Registered: 08/12/16
Posts: 696
Last seen: 10 months, 13 days
Re: Bottle grow I gone and done [Re: synthetik]
    #24020103 - 01/18/17 02:02 PM (7 years, 12 days ago)


Back in town. Looks like the LI is outpacing the wedges but so far so good?


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InvisibleComebackKid
Multispore Enthusiast
Male User Gallery


Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
Trusted Cultivator
Re: Bottle grow I gone and done [Re: Dactylium]
    #24020356 - 01/18/17 03:17 PM (7 years, 12 days ago)

Dactylium man you fuckin kill me!!
This was such a pleasure to read.

FOUR AT A TIME! :lmafo: I know the struggle. My first pc only fit three quart jars so took me four 1.5 hour cycles for two tubs.

Prestooooooo. Preeeeeeestoooooooo. Twenty three quaaaaaaaaart.... prestooooooooo :lol:


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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Offlineacidninja
student
Male User Gallery


Registered: 09/08/16
Posts: 1,693
Loc: the system Flag
Last seen: 2 months, 10 days
Re: Bottle grow I gone and done [Re: ComebackKid]
    #24020409 - 01/18/17 03:32 PM (7 years, 12 days ago)



--------------------

- DMT
- Liberty Cap Gallery


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OfflineDactylium
Don't touch me I'm sterile


Registered: 08/12/16
Posts: 696
Last seen: 10 months, 13 days
Re: Bottle grow I gone and done [Re: ComebackKid]
    #24020509 - 01/18/17 04:03 PM (7 years, 12 days ago)

I've got a 23qt and it'll fit 6 of my quart grain jars just fine last I checked. But these ziplocs are some fat bastards and I can't lay one on its side if I'm going to leave the lids loose for gas exchange. All I want for my  birthday this year is a couple big ass AAs and some propane burners.
:hank:


Edited by Dactylium (01/18/17 04:05 PM)


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OfflineDactylium
Don't touch me I'm sterile


Registered: 08/12/16
Posts: 696
Last seen: 10 months, 13 days
Re: Bottle grow I gone and done [Re: Dactylium]
    #24030973 - 01/22/17 03:58 PM (7 years, 8 days ago)

Put 5 bottles into fruiting 10 days after inoculation. The wedge bottles are still catching up.


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InvisibleIntelligentxfruit
Earth Hippy


Registered: 01/06/13
Posts: 1,545
Re: Bottle grow I gone and done [Re: Dactylium]
    #24031290 - 01/22/17 05:44 PM (7 years, 8 days ago)

:dancingbacon:


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InvisibleKauaiOrca
Waterman

Registered: 08/12/08
Posts: 3,131
Re: Bottle grow I gone and done [Re: Intelligentxfruit]
    #24031344 - 01/22/17 05:57 PM (7 years, 8 days ago)

Great thread.  Very funny write-up.  I do a lot of those PP5 jars too.  I've got it down to a simpler formula than you ... haha ... RGS cased with verm.  That's it.  No mixing, no fuss.


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

-- Ancient Taoist Master


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OfflineDactylium
Don't touch me I'm sterile


Registered: 08/12/16
Posts: 696
Last seen: 10 months, 13 days
Re: Bottle grow I gone and done [Re: KauaiOrca]
    #24031547 - 01/22/17 07:02 PM (7 years, 8 days ago)

You just fruit right off the grain?


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InvisibleMunchauzen
 User Gallery

Registered: 06/22/11
Posts: 14,342
Trusted Cultivator
Re: Bottle grow I gone and done [Re: wtfcrazymofo]
    #24031569 - 01/22/17 07:08 PM (7 years, 8 days ago)

Quote:

wtfcrazymofo said:
Too bad about the lack of li.



yeah i was wondering about that. doesnt look like any water in the jar?


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OfflineDactylium
Don't touch me I'm sterile


Registered: 08/12/16
Posts: 696
Last seen: 10 months, 13 days
Re: Bottle grow I gone and done [Re: Munchauzen]
    #24031585 - 01/22/17 07:12 PM (7 years, 8 days ago)

Oh that was just a pic of my LI jar after the fact. I decided to document this after I already got going.


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InvisibleKauaiOrca
Waterman

Registered: 08/12/08
Posts: 3,131
Re: Bottle grow I gone and done [Re: Dactylium]
    #24031586 - 01/22/17 07:12 PM (7 years, 8 days ago)

Quote:

Dactylium said:
You just fruit right off the grain?




Yep.  When it's fully colonized just case it with verm ... Fruits really well that way.  I use RGS.  With bottles you can bottom water which makes a huge difference and I think makes the water retention properties of some of the other ingredients less necessary.


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

-- Ancient Taoist Master


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InvisibleMunchauzen
 User Gallery

Registered: 06/22/11
Posts: 14,342
Trusted Cultivator
Re: Bottle grow I gone and done [Re: Dactylium]
    #24031609 - 01/22/17 07:18 PM (7 years, 8 days ago)

Quote:

Dactylium said:
Oh that was just a pic of my LI jar after the fact.




it looks like its still full of all the agar?


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OfflineDactylium
Don't touch me I'm sterile


Registered: 08/12/16
Posts: 696
Last seen: 10 months, 13 days
Re: Bottle grow I gone and done [Re: Munchauzen]
    #24031622 - 01/22/17 07:24 PM (7 years, 8 days ago)

Yeah I didn't bother trying to get any of the wedges into the bottles. The mycelium in the water after a good shake does just fine.


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InvisibleMunchauzen
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Registered: 06/22/11
Posts: 14,342
Trusted Cultivator
Re: Bottle grow I gone and done [Re: Dactylium]
    #24031702 - 01/22/17 07:48 PM (7 years, 8 days ago)

Quote:

Dactylium said:
Yeah I didn't bother trying to get any of the wedges into the bottles. The mycelium in the water after a good shake does just fine.



:facepalm:

the agar is colonized with filamentous mycelium. its also the first to recover as its still got a foodsource. the tek of mine that you referenced specifically replaced an old tek that didn't use the agar for this exact reason. if you are not using the agar, you are not doing yourself any favors and are wasting a considerable amount of your culture.


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