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Jimmyjazz
Black Spirit



Registered: 11/18/14
Posts: 58
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Re: Has anyone attempted anaerobic sterilization? [Re: Roostorf]
#24027590 - 01/21/17 08:46 AM (7 years, 9 days ago) |
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Quote:
Roostorf said: Im new to this, but i have some phoenix spawn that will hopefully be ready soon, im gonna try a few methods and see if i can get something to work. I have a barrel of straw & feathers soaking and i added a bucket of ash from the fireplace,
you only need to soak for 8-24 (24 is too long IMO)hours, but you need to make sure that the water reaches around 11pH for it to work, which IME requires SO MUCH ash. Hydrated lime also works, but at that point why not use a more conventional method? The idea when I did this was that the materials were all free and already at hand.
-------------------- "The Meaning of Life is a play on words"
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MycoFlora
Farmer


Registered: 10/06/14
Posts: 309
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Re: Has anyone attempted anaerobic sterilization? [Re: Jimmyjazz]
#24028824 - 01/21/17 06:00 PM (7 years, 9 days ago) |
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Or to do it as cheaply and effectively as possible. For the amount it costs for one propane run to heat pasteurize you could buy a month's supply of hydrated lime. Lime is very effective and cheap!
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Roostorf
Farming Fisherman


Registered: 11/22/16
Posts: 113
Loc: Humboldt
Last seen: 1 year, 6 months
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Re: Has anyone attempted anaerobic sterilization? [Re: MycoFlora]
#24028916 - 01/21/17 06:46 PM (7 years, 9 days ago) |
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That is a great point worth considering and the yeilds are also higher than steam as well
-------------------- Learning something new everyday Would love to trade medicinal and edible vultures and spores
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Jimmyjazz
Black Spirit



Registered: 11/18/14
Posts: 58
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Re: Has anyone attempted anaerobic sterilization? [Re: Roostorf]
#24030132 - 01/22/17 10:26 AM (7 years, 8 days ago) |
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Quote:
MycoFlora said: Or to do it as cheaply and effectively as possible. For the amount it costs for one propane run to heat pasteurize you could buy a month's supply of hydrated lime. Lime is very effective and cheap!
A very good point. The email was by basic definition out of context, but I wasn't misconstruing anything, it was a long thread so I just put the part where he said it was sterilized. Anyway, he described a method where you use the right amount of soaking fluid to rehydrate the substrate, so you don't have any runoff. It made me think of an experiment to try, where you add the right amount to sterilize and hydrate, and then seal the bag, later inoculating it with LC. This would be more for shiitake or lions mane.
-------------------- "The Meaning of Life is a play on words"
Edited by Jimmyjazz (01/22/17 10:27 AM)
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MycoFlora
Farmer


Registered: 10/06/14
Posts: 309
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Re: Has anyone attempted anaerobic sterilization? [Re: Jimmyjazz]
#24032247 - 01/23/17 12:05 AM (7 years, 7 days ago) |
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Yes on the aloha page there is a link to a diagram of the shower/water pump system for lime baths.
As far as hydrating with a precise amount of water/lime Aleon did a nice write up on this quite some time ago. https://www.shroomery.org/forums/showflat.php/Number/19367080/fpart/1/vc/1 I have tried it myself amd it works well, but not very well for slower species like shiitake. You don't need to seal the bag before hand, it won't contaminate in open air because it is not sterile. Just use grain or sawdust spawn no need for the lc. Again, I did 1000's of lbs of sub with lime in open air with 0 contams.
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Jimmyjazz
Black Spirit



Registered: 11/18/14
Posts: 58
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Re: Has anyone attempted anaerobic sterilization? [Re: MycoFlora]
#24033272 - 01/23/17 01:35 PM (7 years, 7 days ago) |
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Yeah right so the purpose of the experiment with the sealed bag/LC is that in theory it should be ok for shiitakes or lions mane given that it should present the mycelium with substrate with 0 competition. I don't know how well it will work but I am going to give it a shot in a few weeks, so I will post results for consideration.
-------------------- "The Meaning of Life is a play on words"
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Roostorf
Farming Fisherman


Registered: 11/22/16
Posts: 113
Loc: Humboldt
Last seen: 1 year, 6 months
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Re: Has anyone attempted anaerobic sterilization? [Re: Jimmyjazz]
#24033434 - 01/23/17 02:47 PM (7 years, 7 days ago) |
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I have been pondering the idea of mixing a sealed bag significantly drier than youd want, then innoculating with a bunch of extra lc to see if that would speed things along, that might be doable with sealed lime bags if it works
-------------------- Learning something new everyday Would love to trade medicinal and edible vultures and spores
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MycoFlora
Farmer


Registered: 10/06/14
Posts: 309
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Re: Has anyone attempted anaerobic sterilization? [Re: Jimmyjazz]
#24038502 - 01/25/17 12:10 PM (7 years, 5 days ago) |
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You would have a much easier time going lc>grain>bulk sub. Ive seen lc>to bulk sub work, but only for faster species like oysters. Adding lime to the mix then inoculating with a slow running species through lc...youd need a lot of lc just to get it to colonize. It just seems lazy. But if it's just for fun, hell, why not.
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Chwyn
Bacteria Rancher


Registered: 08/21/16
Posts: 1,238
Last seen: 10 months, 12 days
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Re: Has anyone attempted anaerobic sterilization? [Re: MycoFlora]
#24072712 - 02/07/17 03:03 PM (6 years, 11 months ago) |
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Just got done with a straw and alfalfa log soaked in ph 13 wood ash water for 2 days. The log is WAY too wet so it will probably trich out. I might make a thread if I get a flush out of it. Ive never made a straw log before and I usually do steam pasteurization on my substrate bags
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theoldman
Stranger
Registered: 03/17/15
Posts: 3
Last seen: 6 years, 8 months
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Re: Has anyone attempted anaerobic sterilization? [Re: Jimmyjazz]
#24168816 - 03/17/17 12:24 AM (6 years, 10 months ago) |
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Quote:
Jimmyjazz said: nice, what kinda spawn is it?
I was able to colonize Agaricus Blazei successfully using your lime pasteurization method. I had to add soap to aid dispersal of the lime into compost. No soak time was utilized. Ratio of spawn was high, but I did not keep tract of the ratio. I thought it would be a failure.
I attempted today to improve on the tek, but failed to bring my compost up to temp. with the winter weather. I had contaminated compost with pest fungi.
Have you tried any other types beside oyster? I have had mixed results with King Oyster and Shiitake, Popular.
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trscstghst
stranger



Registered: 10/14/07
Posts: 786
Loc: here
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Re: Has anyone attempted anaerobic sterilization? [Re: theoldman]
#24245198 - 04/15/17 12:51 AM (6 years, 9 months ago) |
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I have used the other end of the ph scale to ferment kitchen wates. this was used to feed a worm bin.
afer I began feeding "bokashi" to my worms, I did notice an increase in fungal growth in the casting's tea.
I do examine my teas under a microscope.
-------------------- Why use up the forests which were centuries in the making and the mines which required ages to lay down, if we can get the equivalent of forest and mineral products in the annual growth of the hemp fields? o Henry Ford
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Has anyone attempted anaerobic sterilization? [Re: Jimmyjazz]
#25353164 - 07/28/18 11:13 PM (5 years, 5 months ago) |
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Quote:
Jimmyjazz said: .....My question here is if anyone has done this with a substrate other than straw.
Cold pasteurization.
Wood chips:
https://www.milkwood.net/2013/07/10/making-a-wood-chip-mushroom-garden/
https://www.alohamedicinals.com/low-tech-growing.html
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th.maso


Registered: 07/20/18
Posts: 91
Loc: PNW
Last seen: 4 years, 15 days
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Re: Has anyone attempted anaerobic sterilization? [Re: Jimmyjazz]
#25385692 - 08/13/18 01:15 PM (5 years, 5 months ago) |
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Anyone have any knowledge of high pressure CO2 sterilization? I’m at work and haven’t had a chance to read the paper, but does anyone think there’s viability in it?
https://pdfs.semanticscholar.org/0f0f/2ef32f76893b2fb75d105c41920ba2ae6377.pdf
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Has anyone attempted anaerobic sterilization? [Re: th.maso]
#25385742 - 08/13/18 01:39 PM (5 years, 5 months ago) |
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I've read about it being tried with beer but getting back to atmospheric pressure is the hard part. Any fluid gets force carbonated and then foams like mad on the way back. Also makes carbonic acid.
I would say there's no way it will ever be useful for even a farm type situation
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