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Sundar
Stranger

Registered: 12/15/16
Posts: 12
Loc: Quito - Ecuador
Last seen: 9 months, 20 days
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Growing Psilocybe Caerulescens
#23994709 - 01/09/17 09:46 AM (7 years, 21 days ago) |
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Hi people,
2 days ago I found a beautiful wild patch of Psilocybe Caerulescens (Very powerful species). I putted two caps to drop spores and cloned one nice young fruit into 10 petri dishes.
So, I'll post pictures as soon as there is any growth. Any advice for this specific species? Grain medium?I was thinking to make a casing for fruiting afterwards because the fruits are large
Thanks
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enlightenment
alchemist


Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 8 months, 21 days
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Re: Growing Psilocybe Caerulescens [Re: Sundar]
#23994743 - 01/09/17 10:07 AM (7 years, 21 days ago) |
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You could inoculate some grain jars with your cloned culture and spawn them to straw based substrate (example: 80% straw 20% horse manure).
The search function should give you some references.
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Sundar
Stranger

Registered: 12/15/16
Posts: 12
Loc: Quito - Ecuador
Last seen: 9 months, 20 days
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Re: Growing Psilocybe Caerulescens [Re: Sundar]
#24035213 - 01/24/17 06:06 AM (7 years, 6 days ago) |
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Time for some pictures:
 So this is this first plate. This troubles me. I've no idea whether those bumps are contamination or sclerotia. What do you think?
 And this is the tenth plate. Is looks contamination free apart from the goo growing at the edge of the plate. I'm going to let it grow more and transfer to wheat. Could you recommend a nice grain recipe?
The good news is that plate #3 is growing completely contamination free, pure white mycelium. I didn't take a picture of it to let it grow stress free and let it colonize the entire plate. Then this plate will be transfered to grain and other petri dishes to preserve the fungi. Any other transfer recommended?
Thank you
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Psynonim
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Registered: 01/19/15
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Re: Growing Psilocybe Caerulescens [Re: Sundar]
#24045700 - 01/28/17 04:52 AM (7 years, 2 days ago) |
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Quote:
Sundar said: Time for some pictures:
 So this is this first plate. This troubles me. I've no idea whether those bumps are contamination or sclerotia. What do you think?
 And this is the tenth plate. Is looks contamination free apart from the goo growing at the edge of the plate. I'm going to let it grow more and transfer to wheat. Could you recommend a nice grain recipe?
The good news is that plate #3 is growing completely contamination free, pure white mycelium. I didn't take a picture of it to let it grow stress free and let it colonize the entire plate. Then this plate will be transfered to grain and other petri dishes to preserve the fungi. Any other transfer recommended?
Thank you
Dont wait man. At the beginning transfer as soon as possible coz if there are some bacterias or other contam they will soon catch up with the mycelium. Don't wait till full petri colonisation. Transfer now from the section opposite to the contam. Make 2-4 new dishes from each clean petri. Hell you are so lucky.
If you could add pictures of the mushrooms you have found.
Wicked thread, I'm staying tuned.
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Sundar
Stranger

Registered: 12/15/16
Posts: 12
Loc: Quito - Ecuador
Last seen: 9 months, 20 days
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Re: Growing Psilocybe Caerulescens [Re: Sundar]
#24163882 - 03/15/17 09:32 AM (6 years, 10 months ago) |
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Sorry for the long time away.
So, I transfered the petri dishes to grain jars and it looks good.
I bought morochillo for grain (its a type of corn for feeding ducks and chicken) at 0.25$/lb, cooked it along with gypsum and sterilized. Then I transfered a healthy slice of the agar with the mycelium to the jar. So today its been three weeks since inoculation. let me show you some pictures:

This is the Caerulescens. The mycelium is very slow. Currently the temperatures have dropped a lot than the average in Quito

This is a jar containing Cubensis. The myceliums looks thick and nice.
and I also have jars with a blue Ostreatus, and this mycelium is super fast.
So:
I already did G2G transfer from the blue oyster inside my still-air-box. I don't have oust so I use a sprayer with 5% chlorine to kill everything inside it and let it sit for 10 min. The thing is that after 2 days after the transfer, the grain turned from white to pink, so I think the spray with chlorine might be too strong, as air with chlorine will get trapped inside the jars.
I don't want to kill the psilocybe species so do you think I can bomb the box with just ethanol instead of chlorine? or maybe a mix of both at different proportions.
Please comment on procedures, grain type and possible ways to keep the project
Thank you
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Growing Psilocybe Caerulescens [Re: Sundar]
#24163887 - 03/15/17 09:33 AM (6 years, 10 months ago) |
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Chlorine and alcohol are sanitizer You shouldn't be spraying them in the box unless you love to breathe harsh chemicals for fun
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Sundar
Stranger

Registered: 12/15/16
Posts: 12
Loc: Quito - Ecuador
Last seen: 9 months, 20 days
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Re: Growing Psilocybe Caerulescens [Re: Sundar]
#24163899 - 03/15/17 09:38 AM (6 years, 10 months ago) |
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@Psynonim
I didn't took pictures of the mushrooms when I found them and now I don't think I might found them again. But for your entertainment this is a picture from the internet that look very similar to the ones I found:

I can't be sure that this is the species but it looks the same, had a black spore print, bruised blue when I pressed them and was very psychedelic (I ate just 3/4 of a cap and it was enough). Maybe after fruiting them you can see the mushroom and help me recognize.
Thank you for your interest. I hope you have a very good meal today
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Sundar
Stranger

Registered: 12/15/16
Posts: 12
Loc: Quito - Ecuador
Last seen: 9 months, 20 days
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Re: Growing Psilocybe Caerulescens [Re: Sundar]
#24163901 - 03/15/17 09:40 AM (6 years, 10 months ago) |
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thank you bodhisatta. I hate breathing chlorine, makes me cough like hell. So, I shouldn't spray the inside of the box with anything?
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Growing Psilocybe Caerulescens [Re: Sundar]
#24163911 - 03/15/17 09:43 AM (6 years, 10 months ago) |
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Water or nothing yes.
Look up my SAB tek
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bastard4life
Travel Agent



Registered: 03/12/11
Posts: 955
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Re: Growing Psilocybe Caerulescens [Re: bodhisatta]
#24163932 - 03/15/17 10:00 AM (6 years, 10 months ago) |
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Is dawn in water still a good idea or should i save the soap for cleanup?
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Growing Psilocybe Caerulescens [Re: bastard4life]
#24163937 - 03/15/17 10:02 AM (6 years, 10 months ago) |
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I use a drop of soap into 16.9 oz or 500ml of water.
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Sundar
Stranger

Registered: 12/15/16
Posts: 12
Loc: Quito - Ecuador
Last seen: 9 months, 20 days
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Re: Growing Psilocybe Caerulescens [Re: Sundar]
#24326628 - 05/17/17 09:40 AM (6 years, 8 months ago) |
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Hi people.
So without any Idea I tried to fruit the spawn directly from the jar as in the picture below:

I just opened the fully colonized jar exposing it to fresh air in the room window. Air is pretty much humid right now in Quito so I just filled the jar with water for one day, then drained it leaving 1 cm at the bottom and waited for it to fruit. It's been already to weeks, no signs of pinning an sadly one grain got contaminated with the green devil.
Humbly I accept my ignorance about the subject and I beg wisdom on how to does fruiting work and how to fruit the other jars I have. This Jar was corn inoculated directly from agar. In the other jars it was G2G transfer from one of this jars to wheat and popcorn.
Average temperature right now is 14ºC 57ºF. Maybe that's why the jar did not fruit.
I would be really thankful to any information on how to initiate fruiting
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