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OfflineShroomySeth
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1st Monotub.Golden Teacher.
    #23994580 - 01/09/17 08:17 AM (7 years, 21 days ago)

Just knocked up twelve one liter jars of white millet with 20cc of golden teacher spore solution.

  I used a PC to sterilize the millet, and since I dont have a PC big enough for twelve large jars, I put the millet into a couple spawn bags w/ filter patches.

  Using synthetic filter disc tops on the jars. inoculated through the filter discs and repaired the holes with micropore tape.

Yes, I know I used too much spore solution. Call it an experiment... Not really worried about it as money isn't really an issue, and I have two more spore syringes waiting in the wings (Mazapatec & Penis Envy).  Plus I bought a big enough bag of millet to feed a small nation.

Yes, I also know that you don't generally inoculate through filter discs, but it just turned out to be the easiest way for me.

Also, the pic shows the actual lids on top of the filter discs.  Took the pic before i decided to remove the lids.  Figured the discs and the tape would do the job just fine.



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OfflineKenetic
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Re: 1st Monotub.Golden Teacher. [Re: ShroomySeth]
    #23994597 - 01/09/17 08:26 AM (7 years, 21 days ago)

Why would you ruin a filter like that.  You might as well just have used micropore tape.  To make it worse you put the discs on the wrong side of the jar....

Off to a great start I see


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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Offlineblackout
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Re: 1st Monotub.Golden Teacher. [Re: ShroomySeth]
    #23994631 - 01/09/17 08:50 AM (7 years, 21 days ago)

Quote:

ShroomySeth said:
since I dont have a PC big enough for twelve large jars, I put the millet into a couple spawn bags w/ filter patches.




I don't get it, you have 12 jars pictured. I hope you did not sterilize the grain in bags and then transfer into jars? This can be done successfully if you retreat the jars, they should only need a steaming. Or maybe if done in an SAB or in front of a flow hood, but certainly not advisable, but the empty jars would of course have to be sterile beforehand.

Quote:

ShroomySeth said:
Yes, I know I used too much spore solution. Call it an experiment... Not really worried about it as money isn't really an issue



Money is not the issue about not using too much. People take for granted there will be contams in syringes and so use less decreases the chance of them getting in.


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OfflineGroo
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Re: 1st Monotub.Golden Teacher. [Re: blackout]
    #23994638 - 01/09/17 09:01 AM (7 years, 21 days ago)

Obviously money is not an issue. I love seeing people with money try things its so extensive and such a wonderful failure. Keep up the good work though :-p. You aught to watch the lets grow mushroom series and perhaps you would be less likely to un- nessiairly throw the money you have plenty of out the window. Till then happy mushrooming.


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InvisibleSubnet Mask
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Re: 1st Monotub.Golden Teacher. [Re: Groo]
    #23994696 - 01/09/17 09:41 AM (7 years, 21 days ago)

I used to have my filters under the jar lid.  The filter is never going to dry out and allow contams through.


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OfflineShroomySeth
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Re: 1st Monotub.Golden Teacher. [Re: Groo]
    #23994750 - 01/09/17 10:12 AM (7 years, 21 days ago)

First off, I didn't use too much spore solution on purpose... I used 20cc for my first grow which was 30 PF tek cakes in pint jars, and it worked out great.  So being a noob I thought the same amount of solution for 12 one liter jars was appropriate.  Whats done is done and I'm not too worried about it.  If i get contams or a failed grow, I'll start over.  Simple as that.

Secondly what do you mean i put the discs on the wrong side of the jar? They only fit one way.  As i stated, i decided not to use the metal lids with the filter discs and that pic was taken before i removed the metal lids.. Also, what do you care if i ruin my filter discs by poking holes in them?  I have tons of them and you aren't paying for them.

Thirdly.. Yes i did sterilize the millet in bags then transfer to the jars.  I did soak the jars and rings in a diluted bleach water solution and steamed them. And yes i have watched the Lets Grow Mushrooms series.

Also, constructive criticism is fine, but the rules of this forum clearly state no flaming, and couple of the responses are bordering on just that. 

It's all a learning experience, and If I'm not upset by my mistakes, I'm not sure why anyone else should be.  At the very least, if i should fail, my grow log will be documented and can be used as a "what not to do" example.  Cut me some slack please.

Anyway, heres a pic of what the finished inoculated jars currently look like.



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OfflineKenetic
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Re: 1st Monotub.Golden Teacher. [Re: ShroomySeth]
    #23994782 - 01/09/17 10:26 AM (7 years, 21 days ago)

Your jars are gonna dry out like that....

And like I said, why use an sfd if youre just gonna ruin it? Who cares if you can afford to, thats beside the point....

If yoy want to inject through the lid use polyfil or create a ship


Edited by Kenetic (01/09/17 11:11 AM)


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Offlineblackout
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Re: 1st Monotub.Golden Teacher. [Re: ShroomySeth]
    #23994835 - 01/09/17 10:47 AM (7 years, 21 days ago)

Quote:

ShroomySeth said:
First off, I didn't use too much spore solution on purpose... I used 20cc for my first grow which was 30 PF tek cakes in pint jars, and it worked out great.


That sounds like you did purposely use more than recommended. If you have trouble squirting small amounts let me know and I will post tips.

PF jars are more forgiving than grain jars. Contams are thought to be less likely to grow or take over PF jars, rice is thought to be more selective about what grows on it. PF jars are not shaken so if there is a small spot of contams it cannot readily spread throughout the jar.


Quote:

ShroomySeth said:
Also, what do you care if i ruin my filter discs by poking holes in them?  I have tons of them and you aren't paying for them.



People give a shit about others. I imagine we have had hundreds of potentially good growers who might have had great new ideas put off in the early stages of growing, and so gave up all hope about growing or thought it just wasn't for them.


Quote:

ShroomySeth said:
Thirdly.. Yes i did sterilize the millet in bags then transfer to the jars.  I did soak the jars and rings in a diluted bleach water solution and steamed them. And yes i have watched the Lets Grow Mushrooms series.



How did you transfer the millet to the jars? if done in open air they are definitely contaminated. Also how long did you sterilize the bags? they take a long time.

In lets grow mushrooms he shows how to do the jars with discs, the metal disc with small holes sits on the jar, then the filter disc on top, the the screw on ring. The jars will lose too much moisture as said above, also the discs risk getting wet from the grains and contams growing through the discs.

If you transferred in open air, and/or did not PC your bags long enough I would re-sterilize the jars. I would take the caps off and put the metal lids on underneath and PC them.

I am a big fan of experimentation but if you are planning anything unusual just run it by people first, surely you put some value your own time.


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OfflineThedenthead
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Re: 1st Monotub.Golden Teacher. [Re: blackout]
    #23994884 - 01/09/17 11:09 AM (7 years, 21 days ago)

Hit the reset button bud.


--------------------
Nothing but my meandering thoughts.

An ounce of prevention is worth a pound of cure.


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OfflineShroomySeth
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Re: 1st Monotub.Golden Teacher. [Re: blackout]
    #23994894 - 01/09/17 11:12 AM (7 years, 21 days ago)

Thank you blackout, your response was well thought out and constructive.. I appreciate that.

I sterilizated the millet in the PC for 90 minutes at 15psi. Yes i transfered open air, but with as many precautions as humanly possible, lysol around work area, gloves, no hvac, large sterilized metal spoon, surgical mask, etc.

I know it probably wasn't enough, but I'm takimg the wait and see approach now as it's already done.


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OfflineKenetic
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Re: 1st Monotub.Golden Teacher. [Re: ShroomySeth]
    #23994910 - 01/09/17 11:19 AM (7 years, 21 days ago)

Good luck.  Just for the record Im not being a dick at all, Im at work on a phone so my responses are pretty short....

Youll have much better results if you pick a tek and follow it until you get more experience.  I wouldnt get your hopes up gor this grow though


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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OfflineShroomySeth
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Re: 1st Monotub.Golden Teacher. [Re: Kenetic]
    #23995104 - 01/09/17 12:30 PM (7 years, 21 days ago)

Quote:

kenetic said:
Youll have much better results if you pick a tek and follow it until you get more experience.  I wouldnt get your hopes up gor this grow though




Yeah i hear ya.  I cherry picked certain aspects from a few different teks for this one.  Mostly just an experiment, but you're probably right.


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OfflineGroo
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Re: 1st Monotub.Golden Teacher. [Re: ShroomySeth]
    #23996854 - 01/09/17 10:35 PM (7 years, 20 days ago)

Quote:

ShroomySeth said:
Thank you blackout, your response was well thought out and constructive.. I appreciate that.

I sterilizated the millet in the PC for 90 minutes at 15psi. Yes i transfered open air, but with as many precautions as humanly possible, lysol around work area, gloves, no hvac, large sterilized metal spoon, surgical mask, etc.

I know it probably wasn't enough, but I'm takimg the wait and see approach now as it's already done.




Yes i transfered open air, but with as many precautions as humanly possible Like what spraying a deathcloud of lysol in hte air? Get real build a Still air box https://www.shroomery.org/forums/showflat.php/Number/4359637 Don't do any open air stuff you wasting resources.


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Invisibleberlinmyc
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Re: 1st Monotub.Golden Teacher. [Re: Groo]
    #23997251 - 01/10/17 03:25 AM (7 years, 20 days ago)

I've read some members talk about success in open air but I wouldn't risk it. A still air box is super cheap and easy. But if you want to open air it you should use Ozium cause it's for airborne bacteria. Lysol is just for surfaces.


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Offlinewtfcrazymofo
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Re: 1st Monotub.Golden Teacher. [Re: ShroomySeth]
    #23997285 - 01/10/17 04:04 AM (7 years, 20 days ago)

Op sounds like you got some future contam-ed ms grain jars.(Please post future pics) While your waiting for the contams to show up why don't you just whip up some agar, take a few transfers, get a clean dish, whip up some more grain jars, put the sfd on the top and grain inside about 3/4 full(before pc'ing), and knock each jar up with a wedge(not popping any holes in your sfd's).


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




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Offlinesynthetik
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Re: 1st Monotub.Golden Teacher. [Re: wtfcrazymofo]
    #23997290 - 01/10/17 04:13 AM (7 years, 20 days ago)

^ this... seems like the best way to go bud, isolate what you want to grow


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OfflineShroomySeth
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Re: 1st Monotub.Golden Teacher. [Re: wtfcrazymofo]
    #23997353 - 01/10/17 05:41 AM (7 years, 20 days ago)

Quote:

wtfcrazymofo said:
Op sounds like you got some future contam-ed ms grain jars.(Please post future pics) While your waiting for the contams to show up why don't you just whip up some agar, take a few transfers, get a clean dish, whip up some more grain jars, put the sfd on the top and grain inside about 3/4 full(before pc'ing), and knock each jar up with a wedge(not popping any holes in your sfd's).




Sound advice.  Never tried agar before but I'm willing to give it a shot.  Is there a favorite simple agar tek?


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OfflineTheDuder
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Re: 1st Monotub.Golden Teacher. [Re: ShroomySeth]
    #23997371 - 01/10/17 06:07 AM (7 years, 20 days ago)



--------------------

|-------------------[Ps. Azurescens]------------------------------------------[Ps. Semilanceata]--------------------------------------------[Ps. Allenii]------------------------|


|--------------[Ps. Ovoideocystidiata]------------------------------------------[Ps. Stuntzii]--------------------------------------------[Ps. Baeocystis]----------------------|


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OfflineTh3Issu3
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Re: 1st Monotub.Golden Teacher. [Re: TheDuder]
    #23997400 - 01/10/17 06:35 AM (7 years, 20 days ago)

If money isn't that much of a issue, buy some good stuff. Here's a few things to check out.

Premade flowhood.
Self healing injection ports
All American 930
1000 mediem spawn bags (bulk saves money )
Excalibur dehydrator
Potato dextrose agar
Petri dishes
Staples

I could probably go on for a week, but if you have the money to do it, mite as well do it right!


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OfflineAlCapone2k
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Re: 1st Monotub.Golden Teacher. [Re: Th3Issu3]
    #23997414 - 01/10/17 06:52 AM (7 years, 20 days ago)

You will for sure get a conatmination.

Only 90minutes for PCing + Open Air transfer + wrong lid build + ms spore + no SAB and so on and so on.

Buddy, I don't mean it offensive, but there are so many contamns vectors in your work, it can't go well.

Just start again, throw away the millet before they are so conatminated that you have to throw away the whole jar, clean out the jars well and start over again.


You don't have to sterilize all the jars at the same time. You could start at the morning, sterilze the first batch of jars for 2 hours, then the next batch and so on until all the jars are sterilized. Let them cool for over night and start inoculation the day after.

This will minimize your conatamn risk.

Follow the teks of LGM and start with agar or do PF-Tek until you have learned to work with agar. MS syringes aren't a good idea for grain jars, believe us! :smile:


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


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