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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: The Cultivation Tip of the Day thread [Re: Lobi]
#24011201 - 01/15/17 01:14 AM (7 years, 15 days ago) |
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Rolling boil, dump on rye, soak 1hour~ strain, load, and PC 90 mins 16psi
Mush- did you wait a day or so after making those fooman jars for the grains to absorb the moisture?
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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Panarchist
Cyanescendant



Registered: 04/02/16
Posts: 432
Loc: Copeland
Last seen: 7 months, 17 days
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Re: The Cultivation Tip of the Day thread [Re: van hatton] 1
#24013230 - 01/15/17 09:28 PM (7 years, 15 days ago) |
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IME, colonized grain>agar produces much more robust and fast-growing mycelium than MS>agar.
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Lobi
Bushido



Registered: 02/06/16
Posts: 1,231
Loc: PNW
Last seen: 4 months, 29 days
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Re: The Cultivation Tip of the Day thread [Re: Panarchist]
#24013277 - 01/15/17 09:44 PM (7 years, 15 days ago) |
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Quote:
Panarchist said: IME, colonized grain>agar produces much more robust and fast-growing mycelium than MS>agar.
 I like to do ms to small amounts of grain and then shake @ 30%. once recovered you can just take one of the colonizing grains to agar.
-------------------- The bonds and ties of the life we know break easily. But through eternity one bond remains; the bond of fellowship. The fellowship of atoms, of star dust in its endless flight, of suns and worlds, of gods and men. The clasped hands of comradeship unite in a bond eternal; the fellowship of spirit. - My High Quality Lo-Fi Beats - - MushroomCultivation Compendium - - Doing Bulk w/ No PC - more about my music
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Adden

Registered: 06/04/03
Posts: 39,201
Loc:
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Re: The Cultivation Tip of the Day thread [Re: Panarchist]
#24013322 - 01/15/17 10:12 PM (7 years, 15 days ago) |
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Well, yeah. There's no spore germination time.. so you save 3 to 7 days.
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psilly the kid
Hedge wizard/ Cultist



Registered: 05/17/16
Posts: 4,615
Loc: Stoprock City
Last seen: 1 year, 3 months
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Re: The Cultivation Tip of the Day thread [Re: Adden]
#24013935 - 01/16/17 07:49 AM (7 years, 14 days ago) |
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I g2g with sterile hemostats super easy to grab a piece with
-------------------- CA weed @ all times unless specified currently this high pebbles suck its turtles all the way down
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Lobi
Bushido



Registered: 02/06/16
Posts: 1,231
Loc: PNW
Last seen: 4 months, 29 days
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Re: The Cultivation Tip of the Day thread [Re: Adden]
#24014351 - 01/16/17 11:06 AM (7 years, 14 days ago) |
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Quote:
Adden said: Well, yeah. There's no spore germination time.. so you save 3 to 7 days.
Also taking mycelium from a high nutrient medium of grain to a low medium of agar will increase vigor. also from ms you will also be isolating as you do this, which will also increase vigor and health, granted you get a good iso.
-------------------- The bonds and ties of the life we know break easily. But through eternity one bond remains; the bond of fellowship. The fellowship of atoms, of star dust in its endless flight, of suns and worlds, of gods and men. The clasped hands of comradeship unite in a bond eternal; the fellowship of spirit. - My High Quality Lo-Fi Beats - - MushroomCultivation Compendium - - Doing Bulk w/ No PC - more about my music
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Corona11
The New Guy



Registered: 12/09/16
Posts: 151
Last seen: 6 years, 2 days
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Re: The Cultivation Tip of the Day thread [Re: usmc0811]
#24014690 - 01/16/17 01:10 PM (7 years, 14 days ago) |
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Quote:
usmc0811 said: 5 Gallon Elastic Opening Paint Strainer Bags work great for rinsing off your perlite without that much of a mess.

I use the smaller ones to strain my agar before i use pastys prepour tek.
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Qlockwork
Novice


Registered: 04/14/14
Posts: 17
Last seen: 2 years, 7 months
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Re: The Cultivation Tip of the Day thread [Re: Lobi]
#24027544 - 01/21/17 08:25 AM (7 years, 9 days ago) |
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Quote:
Lobi said:
Quote:
Adden said: Well, yeah. There's no spore germination time.. so you save 3 to 7 days.
Also taking mycelium from a high nutrient medium of grain to a low medium of agar will increase vigor. also from ms you will also be isolating as you do this, which will also increase vigor and health, granted you get a good iso.
That makes perfect sense, as the mycelium is obviously going to become more aggressive searching for nutrients. So if if i were to do the opposite like take mycelium from a low nutrient medium of agar to a high nutrient medium of grain, yet it still increases with great vigor & aggression, would that make for a promising iso?
Edited by Qlockwork (01/21/17 08:28 AM)
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Orbit
Stranger


Registered: 12/06/16
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Loc: Greatwhitenorth
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Re: The Cultivation Tip of the Day thread [Re: Qlockwork]
#24038094 - 01/25/17 09:04 AM (7 years, 5 days ago) |
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That makes perfect sense, as the mycelium is obviously going to become more aggressive searching for nutrients. So if if i were to do the opposite like take mycelium from a low nutrient medium of agar to a high nutrient medium of grain, yet it still increases with great vigor & aggression, would that make for a promising iso?
What percentage do you use for this. Malt for instance?
-------------------- For Canadian trades PM me.
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: The Cultivation Tip of the Day thread [Re: Orbit]
#24039525 - 01/25/17 06:42 PM (7 years, 5 days ago) |
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2â„…
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: The Cultivation Tip of the Day thread [Re: van hatton]
#24052501 - 01/30/17 06:19 PM (7 years, 5 hours ago) |
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Ok, reposting this in the appropriate thread, I posted it in "true confessions" by accident.
If you don't own a flowhood but love lc's there's a way I've come up with to noc liquid broth with minimum exposure to air, very very quick 'n clean and far less riskier than noccing with agar wedges. Here it is:
-Sterilize a syringe with some distilled water in it, the thinner the needle the better (I use a 23 G needle for this). -Sterilize the liquid broth of your choice, however many ml you want. -Choose a clean plate, I prefer a plate that's at least a third transfer from ms because that'll allow you to see what the fuck's actually going on in the plate more clearly. Choose the spot that looks the cleanest and with the most organized growth, it doesn't necessarily have to be the leading edge. -Prep your SAB as usual, put all these things in it, pre-open the plate (if you use no-pours like me) and the lc jar. -Flame sterilize needle, grab the plate, squirt some drops of water from the needle in the sab to cool it, pierce only once the spot of agar of your choice until you touch the base of the plate with the needle. Now take the syringe and squirt a small jet of water into the lc broth.
Done.
To avoid confusion, you don't have to squirt any water in the plate, none at all, only pierce it once with the cooled needle, that's it.
Pics, these lc's are 1 week old. The metallic balls you see are from some blender bottles they're nice to break the myc apart to speed things up.


I already tested one that's not pictured and it's very clean. I'd like to stress that you only need a few myc cells to get growth, that's all it takes, so you only need to pierce the agar once with a thin needle. You won't see anything in the lc and you'd probably think it won't work, but it will. You'll start to see a tiny fleck that's like a minuscule scrap of paper, that's what you want.
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Changa Alchemist
Shwift sauce lifeguard



Registered: 03/16/16
Posts: 2,707
Loc: 90+% Rh
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Re: The Cultivation Tip of the Day thread [Re: Josex]
#24052685 - 01/30/17 07:28 PM (7 years, 4 hours ago) |
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Quote:
Josex said: Ok, reposting this in the appropriate thread, I posted it in "true confessions" by accident.
If you don't own a flowhood but love lc's there's a way I've come up with to noc liquid broth with minimum exposure to air, very very quick 'n clean and far less riskier than noccing with agar wedges. Here it is:
-Sterilize a syringe with some distilled water in it, the thinner the needle the better (I use a 23 G needle for this). -Sterilize the liquid broth of your choice, however many ml you want. -Choose a clean plate, I prefer a plate that's at least a third transfer from ms because that'll allow you to see what the fuck's actually going on in the plate more clearly. Choose the spot that looks the cleanest and with the most organized growth, it doesn't necessarily have to be the leading edge. -Prep your SAB as usual, put all these things in it, pre-open the plate (if you use no-pours like me) and the lc jar. -Flame sterilize needle, grab the plate, squirt some drops of water from the needle in the sab to cool it, pierce only once the spot of agar of your choice until you touch the base of the plate with the needle. Now take the syringe and squirt a small jet of water into the lc broth.
Done.
To avoid confusion, you don't have to squirt any water in the plate, none at all, only pierce it once with the cooled needle, that's it.
Pics, these lc's are 1 week old. The metallic balls you see are from some blender bottles they're nice to break the myc apart to speed things up.


I already tested one that's not pictured and it's very clean. I'd like to stress that you only need a few myc cells to get growth, that's all it takes, so you only need to pierce the agar once with a thin needle. You won't see anything in the lc and you'd probably think it won't work, but it will. You'll start to see a tiny fleck that's like a minuscule scrap of paper, that's what you want.
This is pretty cool man, I wanna give this a try.
I've just used LME lc in the past. What do you prefer?
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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I use lme at 0,3% strength. You should be fine with 0,2% lme (1 gram for 500 ml of water) and that'll give you a very clear lc and by the time the myc fully colonizes the lc it should almost be as clear as water.
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Shu
Vote for Humanity



Registered: 11/20/11
Posts: 885
Loc: PA, USA
Last seen: 13 days, 2 hours
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Re: The Cultivation Tip of the Day thread [Re: Josex]
#24052737 - 01/30/17 07:43 PM (7 years, 3 hours ago) |
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Quote:
Josex said:
Pics, these lc's are 1 week old. The metallic balls you see are from some blender bottles they're nice to break the myc apart to speed things up.


Blender balls, brilliant!
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Panarchist
Cyanescendant



Registered: 04/02/16
Posts: 432
Loc: Copeland
Last seen: 7 months, 17 days
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Re: The Cultivation Tip of the Day thread [Re: Shu]
#24052845 - 01/30/17 08:28 PM (7 years, 3 hours ago) |
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my preferred implement for taking agar transfers has become an empty spore syringe. the narrow shape of the needle allows you to make much more precise segments and really target a specific area.
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Lobi
Bushido



Registered: 02/06/16
Posts: 1,231
Loc: PNW
Last seen: 4 months, 29 days
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Re: The Cultivation Tip of the Day thread [Re: Shu]
#24052852 - 01/30/17 08:30 PM (7 years, 2 hours ago) |
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That's exactly what I do with LC and agar plates to avoid having a wedge in my LC. It's also a lot easier to see growth. You can also just use any sterile utensil and just scrape off the myc from the plate and swipe it off on the inside edge of the glass lid.
-------------------- The bonds and ties of the life we know break easily. But through eternity one bond remains; the bond of fellowship. The fellowship of atoms, of star dust in its endless flight, of suns and worlds, of gods and men. The clasped hands of comradeship unite in a bond eternal; the fellowship of spirit. - My High Quality Lo-Fi Beats - - MushroomCultivation Compendium - - Doing Bulk w/ No PC - more about my music
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: The Cultivation Tip of the Day thread [Re: Panarchist]
#24052855 - 01/30/17 08:32 PM (7 years, 2 hours ago) |
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Quote:
Panarchist said: my preferred implement for taking agar transfers has become an empty spore syringe. the narrow shape of the needle allows you to make much more precise segments and really target a specific area.
hole lee shit. nice
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: The Cultivation Tip of the Day thread [Re: mushboy]
#24052884 - 01/30/17 08:41 PM (7 years, 2 hours ago) |
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Well a syringe should work for transfers but the thing is that if you transfer a very small sample it's going to take much longer to colonize the plate. I'd imagine it would very useful if you want to isolate strains. With lc's is doesn't matter so much if you only transfer a few cells because you can greatly speed things up by shaking gently if you're poor like me or by using a magnetic stirrer.
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Panarchist
Cyanescendant



Registered: 04/02/16
Posts: 432
Loc: Copeland
Last seen: 7 months, 17 days
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Re: The Cultivation Tip of the Day thread [Re: mushboy]
#24052943 - 01/30/17 09:01 PM (7 years, 2 hours ago) |
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thanks, mushboy. hope everything is going good with your pan projects.
Josex, it does take a little longer but i don't know if it's that much. here's a 3x pan cyan transfer at 18 days of growth. the transfer on this was smaller than a grain of rice. two other transfers from this round grew out even faster but they wound up showing signs of infection.

i first tried it as part of an attempt to pull some mycelium of an infected plate. it worked out so i've just been using it since but i would agree that as the mycelium gets cleaned up it's not really necessary to take such small segments but i just like to get as many as i can.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: The Cultivation Tip of the Day thread [Re: Panarchist]
#24052951 - 01/30/17 09:03 PM (7 years, 2 hours ago) |
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in a few days ill be spawning this half filled quart to some cvg. ill do a mini tray inside a mini mono. i can really control the conditions with those two working together.
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