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OfflineDabrit
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How much more should I let this plate grow before spawning?
    #23993330 - 01/08/17 06:41 PM (7 years, 22 days ago)

Here's a picture of the most aggressive petri dish I have on the go right now of GT variety.

Just wondering how much further I should let it grow before spawning to two master jars?

It's growing at a rapid rate now something like 20% in a day.



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OfflineKenetic
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Re: How much more should I let this plate grow before spawning? [Re: Dabrit]
    #23993339 - 01/08/17 06:43 PM (7 years, 22 days ago)

bout like that


--------------------
Todo Cambia
   

               
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OfflineDabrit
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Re: How much more should I let this plate grow before spawning? [Re: Kenetic]
    #23993349 - 01/08/17 06:45 PM (7 years, 22 days ago)

Quote:

kenetic said:
bout like that




When I cut the wedges to place in the jars do I just cut to the edge of the mycelium growth or just cut to the edge of the agar?

Might be a dumb question but figured I'd ask anyway


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Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume :wink:




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InvisibleAdden
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Re: How much more should I let this plate grow before spawning? [Re: Kenetic]
    #23993351 - 01/08/17 06:46 PM (7 years, 22 days ago)

Just out of curiosity.. sorry OP.. but where would the best place for transfers be? Between 9 and 1?

Or noon and 3?

Cut a tiny sliver out OP, like the size of a grain of rice. Don't have to dig in deep and get something huge.


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OfflineDabrit
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Re: How much more should I let this plate grow before spawning? [Re: Adden]
    #23993366 - 01/08/17 06:49 PM (7 years, 22 days ago)

Quote:

Adden said:
Just out of curiosity.. sorry OP.. but where would the best place for transfers be? Between 9 and 1?

Or noon and 3?




I'm not planning on doing another transfer the culture is clean and is from MS without knowing the specifics of the any strains on the plate there really isn't any point in isolating any further. I was thinking I cut out the whole thing into sections and spawn master jars then do G2G once they are fully colonized.

This is a 1st transfer plate.


--------------------
Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume :wink:




Edited by Dabrit (01/08/17 06:51 PM)


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Offlinewtfcrazymofo
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Re: How much more should I let this plate grow before spawning? [Re: Dabrit]
    #23993475 - 01/08/17 07:27 PM (7 years, 22 days ago)

It is good practice to get at least 1 plate away from the ms plate then go to grains.  If that is a second transfer plate you could do what you want, but not with all the extra naked agar.

You could also wait and see if the plate pins and take a clone. G2g<LI.  With good LI technique one clean plate can become 30 quart jars in about the same amount of time as your 2 masters will take.


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InvisiblebodhisattaMDiscordReddit
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Re: How much more should I let this plate grow before spawning? [Re: Dabrit]
    #23993480 - 01/08/17 07:29 PM (7 years, 22 days ago)

Quote:

Dabrit said:
Quote:

kenetic said:
bout like that




When I cut the wedges to place in the jars do I just cut to the edge of the mycelium growth or just cut to the edge of the agar?

Might be a dumb question but figured I'd ask anyway





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Offlinewtfcrazymofo
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Re: How much more should I let this plate grow before spawning? [Re: bodhisatta]
    #23993509 - 01/08/17 07:37 PM (7 years, 22 days ago)



Do you shake those?


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




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InvisiblebodhisattaMDiscordReddit
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Re: How much more should I let this plate grow before spawning? [Re: wtfcrazymofo]
    #23993521 - 01/08/17 07:42 PM (7 years, 22 days ago)

You bet.


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OfflineDabrit
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Re: How much more should I let this plate grow before spawning? [Re: wtfcrazymofo]
    #23993941 - 01/08/17 10:05 PM (7 years, 21 days ago)

Quote:

wtfcrazymofo said:
It is good practice to get at least 1 plate away from the ms plate then go to grains.  If that is a second transfer plate you could do what you want, but not with all the extra naked agar.

You could also wait and see if the plate pins and take a clone. G2g<LI.  With good LI technique one clean plate can become 30 quart jars in about the same amount of time as your 2 masters will take.




I already transferred away from the ms inoculated agar plate which also looked clean but I transferred anyway. Looks like just cut to the edge of the mycelium is the plan


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OfflineKenetic
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Re: How much more should I let this plate grow before spawning? [Re: Dabrit]
    #23994430 - 01/09/17 05:44 AM (7 years, 21 days ago)

Thats what I do, similar to what bod has shown.  I just cut out the colonized myc, only takes a moment and one fluid motion


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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OfflineDabrit
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Re: How much more should I let this plate grow before spawning? [Re: Kenetic]
    #23994621 - 01/09/17 08:44 AM (7 years, 21 days ago)

Quote:

kenetic said:
Thats what I do, similar to what bod has shown.  I just cut out the colonized myc, only takes a moment and one fluid motion




Ok sweet. On to spawn jars it is.


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Invisibleazur
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Re: How much more should I let this plate grow before spawning? [Re: Dabrit]
    #23994703 - 01/09/17 09:44 AM (7 years, 21 days ago)

Spawning?
Nomenclature...


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OfflineDabrit
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Re: How much more should I let this plate grow before spawning? [Re: azur]
    #23999830 - 01/10/17 10:59 PM (7 years, 19 days ago)

Quote:

azur said:
Spawning?
Nomenclature...




Ok dude what is the preferred nomenclature? Grain Spawn? Am I out of my element?


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InvisibleAdden
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Re: How much more should I let this plate grow before spawning? [Re: Dabrit]
    #23999837 - 01/10/17 11:02 PM (7 years, 19 days ago)

Inoculating grain jars with an agar wedge, so when they're fully colonized you can spawn them to CVG or whatever.


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OfflineDabrit
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Re: How much more should I let this plate grow before spawning? [Re: Adden]
    #23999860 - 01/10/17 11:15 PM (7 years, 19 days ago)

Quote:

Adden said:
Inoculating grain jars with an agar wedge, so when they're fully colonized you can spawn them to CVG or whatever.




No I get that that's the name of the actual process I am about to endeavor. And I misspoke but I'm sure Azur knew what I meant lol.

I was expecting a Big Lebowski quote retort as the only time I've ever really heard that word used was in that movie.

" DUDE THE CHINA MAN IS NOT THE ISSUE HERE.... Also dude china man is not the preferred nomenclature...Asian American please. "

etc etc fuck I love that movie


--------------------
Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume :wink:




Edited by Dabrit (01/10/17 11:17 PM)


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OfflineOrbit
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Re: How much more should I let this plate grow before spawning? [Re: Dabrit]
    #23999901 - 01/10/17 11:33 PM (7 years, 19 days ago)

The reason to let it grow out is to prove the outer edge is clean. If you are just cutting a wedge out of the middle you should be fine. Let it grow out use all of it later.


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OfflineKenetic
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Re: How much more should I let this plate grow before spawning? [Re: Orbit]
    #24000243 - 01/11/17 05:21 AM (7 years, 19 days ago)

?


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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Offlinesynthetik
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Re: How much more should I let this plate grow before spawning? [Re: Kenetic]
    #24000353 - 01/11/17 06:53 AM (7 years, 19 days ago)

I got the reference... hard to tell jokes though a damn keyboard... question did you decide to to the lc/li route?


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OfflineDabrit
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Re: How much more should I let this plate grow before spawning? [Re: Orbit]
    #24000554 - 01/11/17 09:06 AM (7 years, 19 days ago)

Quote:

synthetik said:
I got the reference... hard to tell jokes though a damn keyboard... question did you decide to to the lc/li route?




Nah right now I can only rock 2-3 monotubs max with the space I have. So I'm just creating 2 master jars per variety then G2G to get 6 quarts per tub. Once done fruiting will clone the best looking specimens and hopefully confirm potency and then clone to Agar and then grow out The best looking sectors on the plate one by one to find the best one and finally hopefully end up with a master culture I can use going forward.

I've heard LC is risky and can be hard to see contaminants like you can on agar to be honest the method hasn't interested me at all.

Quote:

Orbit said:
The reason to let it grow out is to prove the outer edge is clean. If you are just cutting a wedge out of the middle you should be fine. Let it grow out use all of it later.




I'm not sure this really makes sense seeing as the whole point of agar is to isolate mycelium away from bacteria and molds etc as they are easy to see. Also If you are doing a transfer, you would usually cut away a small section of mycelium away from the leading edge of the growth as it's the newest and most aggressive. :shrug:


--------------------
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